DK2295556T3 - Lipolytiske enzymvarianter og fremgangsmåde til fremstilling deraf. - Google Patents

Lipolytiske enzymvarianter og fremgangsmåde til fremstilling deraf. Download PDF

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Publication number
DK2295556T3
DK2295556T3 DK10182711.1T DK10182711T DK2295556T3 DK 2295556 T3 DK2295556 T3 DK 2295556T3 DK 10182711 T DK10182711 T DK 10182711T DK 2295556 T3 DK2295556 T3 DK 2295556T3
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DK10182711.1T
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Allan Svendsen
Jesper Vind
Hans Peter Heldt-Hansen
Luise Christiansen
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Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Claims (6)

  1. LIPOLYTISKE ENZYMVARIANTER OG FREMGANGSMÅDE TIL FREMSTILLING DERAF
    1. Lipolytisk enzym, som: a) har en aminosyresekvens, der udviser mindst 90% identitet til den i SEQ ID NO: 7; og b) har phospholipaseaktivitet og c) har et forhold mellem lysophospholipase og phospholipase på mindre end 1000 og d) omfatter mindst én aminosyreændring svarende til position H257W, S142A, V157D+S271P, S80T+A127T, D263G, Y21W, S80T, R274A+275YRSAESVDKR eller R274A+275YRSAESVDKAATMTDAELEKKLNSYVQMDKEYVKNNQARS i SEQ ID NO: 7; og hvor forholdet mellem lysophospholipase og phospholipase er defineret som lysophospholipaseaktiviteten divideret med phospholipaseaktiviteten, hvor phospholipaseaktivitet er bestemt ved 30°C ved anvendelse af 4% (vægt/volumen) lecithin i 50mM natriumacetat, 5mM CaCI2, pH 5,0, og lysophospholipaseaktivitet er bestemt ved 30°C ved anvendelse af 1% (vægt/volumen) lysolecithin i 50mM natriumacetat, 5mM CaCI2, pH 5,0.
  2. 2. Lipolytisk enzym ifølge krav 1, som har et forhold mellem lysophospholipase og phospholipase på mindre end 1000, og som omfatter en aminosyresekvens, der er afledt fra en Fusarium-lipase der omfatter mindst én aminosyreændring svarende til SEQ ID NO: 7: a. H257W; b. S142A; c. V157D+S271P; d. S80T+A127T; e. D263G; f. Y21W; g. S80T; h. R274A+275YRSAESVDKR; i. R274A+275YRSAESVDKAATMTDAELEKKLNSYVQMDKEYVKNNQARS; og hvor forholdet mellem lysophospholipase og phospholipase er defineret som lysop-hospholipaseaktiviteten divideret med phospholipaseaktiviteten, hvor phospholipase-aktivitet er bestemt ved 30°C ved anvendelse af 4% (vægt/volumen) lecithin i 50mM natriumacetat, 5mM CaC^, pH 5,0, og lysophospholipaseaktivitet er bestemt ved 30°C ved anvendelse af 1% (vægt/volumen) lysolecithin i 50mM natriumacetat, 5mM CaCI2, pH 5,0.
  3. 3. Fremgangsmåde til fremstilling af en dej eller et bagt produkt fremstillet af dej, hvilken fremgangsmåde omfatter tilsætning af det lipolytiske enzym ifølge et hvilket som helst af kravene 1-2 til dejen.
  4. 4. Anvendelse af det lipolytiske enzym ifølge et hvilket som helst af kravene 1-2 ved fremstilling af pasta.
  5. 5. Anvendelse af det lipolytiske enzym ifølge et hvilket som helst af kravene 1-2 ved fremstilling af nudler.
  6. 6. Anvendelse af det lipolytiske enzym ifølge et hvilket som helst af kravene 1-2 ved ostefremstilling.
DK10182711.1T 2002-01-16 2003-01-16 Lipolytiske enzymvarianter og fremgangsmåde til fremstilling deraf. DK2295556T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200200074 2002-01-16
EP03701473A EP1468084A1 (en) 2002-01-16 2003-01-16 Lipolytic enzyme variants and method for their production

Publications (1)

Publication Number Publication Date
DK2295556T3 true DK2295556T3 (da) 2015-01-26

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US (2) US20050255544A1 (da)
EP (2) EP2295556B1 (da)
AU (1) AU2003203139B2 (da)
CA (1) CA2467415C (da)
DK (1) DK2295556T3 (da)
WO (1) WO2003060112A1 (da)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
EP1433852B1 (en) 1997-04-09 2006-06-28 Danisco A/S Use of lipase for improving doughs and baked products
EP2278000A1 (en) * 1999-03-31 2011-01-26 Novozymes A/S Lipase variant
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
MXPA05007654A (es) 2003-01-17 2005-09-30 Danisco Metodo.
EP3323887A3 (en) 2003-01-17 2018-09-12 DuPont Nutrition Biosciences ApS Method for the in situ production of an emulsifier in a foodstuff
EP2290057A3 (en) 2003-05-09 2011-08-03 Novozymes A/S Variant lipolytic enzymes
US7754460B2 (en) * 2003-12-03 2010-07-13 Danisco Us Inc. Enzyme for the production of long chain peracid
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
EA014985B1 (ru) 2004-07-16 2011-04-29 Даниско А/С Способ ферментативного рафинирования пищевых масел гидратацией и применение липидацилтрансферазы для осуществления способа
EP2298872A3 (en) 2004-09-30 2011-08-10 Novozymes A/S Polypeptides having lipase activity and polynucleotides encoding same
EP1976967A2 (en) * 2006-01-23 2008-10-08 The Procter and Gamble Company Detergent compositions
US20120141630A1 (en) * 2006-02-06 2012-06-07 Novozymes A/S Screening Method
DE102006046857A1 (de) 2006-10-02 2008-04-03 Ab Enzymes Gmbh Klonierung, Expression und Verwendung saurer Lysophospholipasen
DE102006046719A1 (de) 2006-10-02 2008-04-03 Ab Enzymes Gmbh Klonierung, Expression und Verwendung saurer Phospholipasen
US8273348B2 (en) * 2006-12-21 2012-09-25 Novozymes A/S Lipase variants for pharmaceutical use
WO2008142433A1 (en) * 2007-05-24 2008-11-27 Loders Croklaan B.V. Process for producing compositions comprising tocoherols and tocotrienols
DE102009051013A1 (de) 2009-10-28 2011-06-09 Ab Enzymes Gmbh Klonierung, Expression und Verwendung saurer Phospholipasen
ES2546630T3 (es) 2012-01-30 2015-09-25 Dsm Ip Assets B.V. Alfa-amilasa
DK3211074T3 (da) * 2012-02-03 2020-06-02 Novozymes As Lipasevarianter og polynukleotider, som koder for dem
CN110093330B (zh) * 2012-10-12 2023-11-28 丹尼斯科美国公司 包含脂解酶变体的组合物和方法
JP6548679B2 (ja) 2014-07-08 2019-07-24 キャラヴァン イングリーディエンツ インク． 糖を生成しかつテクスチャーを改善するベーカリー方法およびそれから形成される製品
BE1022042B1 (nl) 2014-09-29 2016-02-08 Puratos Nv Verbeterde cakebeslagsoorten
EP3280818A2 (en) 2015-04-07 2018-02-14 Novozymes A/S Methods for selecting enzymes having lipase activity
CN110129299B (zh) * 2016-06-02 2021-08-03 天津科技大学 磷脂酶d及其应用
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
US10918113B2 (en) 2016-12-21 2021-02-16 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114940A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
AU2018387151B2 (en) * 2017-12-19 2024-05-02 International N&H Denmark Aps Improved enzymatic modification of phospholipids in food
CN111378585B (zh) * 2018-12-28 2023-06-16 丰益(上海)生物技术研发中心有限公司 用于表达外源基因的毕赤酵母突变株
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
US20250304938A1 (en) * 2022-05-16 2025-10-02 Dsm Ip Assets B.V. Lipolytic enzyme variants

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3368903A (en) * 1966-02-18 1968-02-13 Vanderbilt Co R T Baked goods dough and method
JPS6030488B2 (ja) 1982-11-10 1985-07-17 協和醗酵工業株式会社 生地の改良剤およびそれを含有してなる生地
US5352594A (en) * 1984-05-29 1994-10-04 Genecor, Inc. Selection and method of making enzymes for perhydrolysis system and for altering substrate specificity, specific activity and catalytic efficiency
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
JPS644288A (en) 1987-06-25 1989-01-09 Nippon Electric Kagoshima Ltd Preparation of pure water
DE3854249T2 (de) 1987-08-28 1996-02-29 Novonordisk As Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen.
ES2032000T3 (es) 1987-12-03 1993-01-01 Unilever Nv Un procedimiento para la preparacion de una emulsion de agua y aceite.
JP2794574B2 (ja) 1988-08-11 1998-09-10 昭和産業株式会社 リゾレシチンの製造方法
JP2877439B2 (ja) 1989-05-17 1999-03-31 協和醗酵工業株式会社 卵の改質方法
DK46693D0 (da) 1993-04-23 1993-04-23 Novo Nordisk As
DK0548228T3 (da) 1990-09-13 1999-05-10 Novo Nordisk As Lipasevarianter
US5892013A (en) 1990-09-13 1999-04-06 Novo Nordisk A/S Lipase variants
US5869438A (en) 1990-09-13 1999-02-09 Novo Nordisk A/S Lipase variants
JPH0687751B2 (ja) 1990-09-26 1994-11-09 辻製油株式会社 高濃度のリゾホスファチジルコリンを含むリゾレシチンを採取する方法
GB9114734D0 (en) 1991-07-09 1991-08-28 Univ London Process for modifying proteins
PT575133E (pt) 1992-06-16 2002-11-29 Sankyo Co Nova fosfolipase a1 processo para a sua preparacao e sua utilizacao
ES2087646T3 (es) 1992-07-27 1996-07-16 Gist Brocades Nv Producto enzimatico y metodo para mejorar la calidad del pan.
GB9216387D0 (en) 1992-07-31 1992-09-16 Unilever Plc Enzymatic detergent compositions
DK104592D0 (da) 1992-08-21 1992-08-21 Novo Nordisk As Fremgangsmaade
JPH06113845A (ja) 1992-10-06 1994-04-26 Asahi Chem Ind Co Ltd 高モノグリセリド分解性を有する改良リパーゼ及びその製造法
WO1994014964A1 (en) 1992-12-23 1994-07-07 Unilever N.V. Modified cutinases, dna, vector and host
DE69313102T2 (de) 1993-06-11 1997-12-11 Nestle Sa Zusammensetzung für Wärmestabilisierung von Proteinen und Produkt daraus
DK76893D0 (da) 1993-06-28 1993-06-28 Novo Nordisk As
JP4068142B2 (ja) 1995-08-11 2008-03-26 ノボザイムス アクティーゼルスカブ 新規の脂肪分解酵素
JP3791058B2 (ja) 1996-07-30 2006-06-28 日本油脂株式会社 リゾレシチンの製造方法
CA2267647C (en) 1996-09-30 2007-04-10 Genencor International, Inc. Esterases, dna encoding therefor and vectors and host cells incorporating same
DE69716711T3 (de) 1996-12-09 2010-06-10 Novozymes A/S Verringerung von phosphorhaltigen Substanzen in Speiseölen mit hohem Anteil an nicht-hydratisierbarem Phosphor unter Verwendung einer Phospholipase, eine Phospholipase aus fädenförmigen Pilz, welche eine Phospholipase A und/oder B-Aktivität aufweist
US6103505A (en) 1996-12-09 2000-08-15 Novo Nordisk A/S Method for reducing phosphorus content of edible oils
DE19701348A1 (de) 1997-01-16 1998-07-23 Roehm Gmbh Protein mit Phospholipaseaktivität
US5821102A (en) 1997-01-21 1998-10-13 Novo Nordisk Biotech Inc. Nucleic acids encoding polyeptides having absidia lipase activity
JP3853464B2 (ja) 1997-04-08 2006-12-06 辻製油株式会社 植物性リゾレシチンの製造法
EP1433852B1 (en) 1997-04-09 2006-06-28 Danisco A/S Use of lipase for improving doughs and baked products
DK1073339T4 (da) 1998-04-20 2008-08-18 Novozymes As Fremstilling af dej og bagte produkter
US6265197B1 (en) * 1998-07-02 2001-07-24 Novozymes A/S Krogshoejvej Starch debranching enzymes
EP1131416B1 (en) 1998-11-27 2009-09-02 Novozymes A/S Lipolytic enzyme variants
CA2715086A1 (en) * 1998-11-27 2000-06-08 Novozymes A/S Lipolytic enzyme variants
US7312062B2 (en) * 1998-11-27 2007-12-25 Novozymes A/S Lipolytic enzyme variants
DE60018267T3 (de) 1999-03-16 2014-02-06 Novozymes A/S Verfahren zur herstellung von käse
EP1057415A1 (en) 1999-06-04 2000-12-06 Societe Des Produits Nestle S.A. Lipase-treated pasta and manufacturing process
WO2001018163A1 (en) * 1999-09-03 2001-03-15 The Procter & Gamble Company Detergent composition having granular cyclodextrin
US6146869A (en) 1999-10-21 2000-11-14 Novo Nordisk Biotech, Inc. Polypeptides having phospholipase B activity and nucleic acids encoding same
BR0016068A (pt) 1999-12-03 2002-08-06 Danisco Método para melhorar a qualidade da massa e do pão
CA2406621A1 (en) 2000-04-28 2001-11-08 Novozymes A/S Protein variants having modified immunogenicity
EP2119773A1 (en) 2000-06-26 2009-11-18 Novozymes A/S Lipolytic enzymes from strains of fusarium and acremonium

Also Published As

Publication number Publication date
EP2295556A3 (en) 2011-06-29
AU2003203139B2 (en) 2007-11-08
AU2003203139A1 (en) 2003-07-30
US20050255544A1 (en) 2005-11-17
US9115346B2 (en) 2015-08-25
WO2003060112A1 (en) 2003-07-24
CA2467415A1 (en) 2003-07-24
US20110165655A1 (en) 2011-07-07
EP2295556A2 (en) 2011-03-16
EP1468084A1 (en) 2004-10-20
CA2467415C (en) 2014-03-11
EP2295556B1 (en) 2014-10-29

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