DK2310520T3 - Fremgangsmåde til reduktion af mængden af sterylglycosid i en olie eller fedt og/eller et biobrændsel - Google Patents

Fremgangsmåde til reduktion af mængden af sterylglycosid i en olie eller fedt og/eller et biobrændsel Download PDF

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DK2310520T3
DK2310520T3 DK09786077.9T DK09786077T DK2310520T3 DK 2310520 T3 DK2310520 T3 DK 2310520T3 DK 09786077 T DK09786077 T DK 09786077T DK 2310520 T3 DK2310520 T3 DK 2310520T3
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oil
fat
phospholipase
biofuel
steryl
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DK09786077.9T
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Jorn Borch Soe
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Dupont Nutrition Biosci Aps
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
    • C07J17/005—Glycosides
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/006—Refining fats or fatty oils by extraction
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/02—Refining fats or fatty oils by chemical reaction
    • C11B3/04—Refining fats or fatty oils by chemical reaction with acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/02—Refining fats or fatty oils by chemical reaction
    • C11B3/06—Refining fats or fatty oils by chemical reaction with bases
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
    • C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
    • C11C3/003—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fatty acids with alcohols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P30/00—Technologies relating to oil refining and petrochemical industry
    • Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Fats And Perfumes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Edible Oils And Fats (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (23)

  1. FREMGANGSMÅDE TIL REDUKTION AF MÆNGDEN AF STERYLGLYCOSIDI EN OLIE ELLER FEDT OG/ELLER ET BIOBRÆNDSEL
    1. Fremgangsmåde til reduktion af mængden af sterylglycosid i en olie eller fedt og/eller et biobrændsel, hvilken fremgangsmåde omfatter blanding af ét eller flere enzymer med en olie eller fedt, der omfatter sterylglycosid, hvor det ene eller flere enzymer omfatter en amyloglucosidase; således at det ene eller flere enzymer nedbryder sterylglycosidet, og hvor mindst 20 % af sterylglycosidet i olien eller fedtet og/eller biobrændslet fjernes.
  2. 2. Fremgangsmåde ifølge krav 1, hvor olien er en vegetabilsk olie.
  3. 3. Fremgangsmåde ifølge krav 2, hvor olien er udvalgt fra: rapsfrøolie, rapsolie, soja- (sojabønne-) olie, risklidolie, palmeolie, majsolie, bomuldsfrøolie, solsikkeolie, saflorolie, nasturtiumfrøolie, sennepsfrøolie, olivenolie, sesamolie, jordnøddeolie, babassunøddeolie, kastorolie, palmekemeolie, rapsfrøolie med lavt erucasyreindhold, lupinolie, jatrophaolie, kokosolie, linolie, natlysolie, jojobaolie, sheanøddeolie eller sæddodderolie.
  4. 4. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor biobrændslet er en biodiesel.
  5. 5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor fremgangsmåden endvidere omfatter et trin med afkogning.
  6. 6. Fremgangsmåde ifølge krav 5, hvor afkogningen er enzymatisk afkogning.
  7. 7. Fremgangsmåde ifølge krav 6, hvor sterylglycosidet nedbrydes før, under eller efter trinnet med afkogning.
  8. 8. Fremgangsmåde ifølge et hvilket som helst af kravene 5-7, hvor det ene eller flere enzymer blandes med olie eller fedt og vand under trinnet med afkogning.
  9. 9. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor fremgangsmåden endvidere omfatter et trin med interesterificering.
  10. 10. Fremgangsmåde ifølge krav 9, hvor trinnet med interesterificering er et enzymatisk interesterificeringstrin.
  11. 11. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor fremgangsmåden endvidere omfatter et trin med centrifugering.
  12. 12. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor fremgangsmåden endvidere omfatter et enzym, der er udvalgt fra en lipid acyltransferase, en phospholipase eller kombinationer deraf.
  13. 13. Fremgangsmåde ifølge krav 12, hvor phospholipasen er en phospholipase Al, en phospholipase A2, en phospholipase B, en phospholipase C, en phospholipase D eller en kombination deraf.
  14. 14. Anvendelse af ét eller flere enzymer i en olie eller fedt og/eller et biobrændsel til reduktion af mængden af sterylglycosid, hvor det ene eller flere enzymer omfatter en amyloglucosidase, og hvor mindst 20 % af sterylglycosidet i olien eller fedt og/eller biobrændsel er fjernet.
  15. 15. Anvendelse ifølge krav 14, hvor mindst 50 % af sterylglycosidet i olien eller fedt er fjernet.
  16. 16. Anvendelse ifølge krav 14, hvor mindst 80 % af sterylglycosidet i olie eller fedt er fjernet.
  17. 17. Anvendelse ifølge et hvilket som helst af kravene 14-16, hvor olien er en vegetabilsk olie.
  18. 18. Anvendelse ifølge krav 17, hvor den vegetabilske olie er en sojaolie.
  19. 19. Anvendelse ifølge et hvilket som helst af kravene 14-17, hvor biobrændslet er en biodiesel.
  20. 20. Anvendelse ifølge et hvilket som helst af kravene 14-18, hvor anvendelsen endvidere omfatter et enzym, der er udvalgt fra en lipid acyltransferase, en phospholipase eller kombinationer deraf.
  21. 21. Anvendelse ifølge krav 20, hvor phospholipasen er en phospholipase Al, en phospholipase A2, en phospholipase B, en phospholipase C, en phospholipase D eller en kombination deraf.
  22. 22. Anvendelse ifølge et hvilket som helst af kravene 14 til 21, hvor anvendelsen sker i kombination med afkogning.
  23. 23. Anvendelse ifølge krav 22, hvor afkogningen er enzymatisk afkogning.
DK09786077.9T 2008-07-09 2009-07-08 Fremgangsmåde til reduktion af mængden af sterylglycosid i en olie eller fedt og/eller et biobrændsel DK2310520T3 (da)

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Application Number Priority Date Filing Date Title
GBGB0812559.3A GB0812559D0 (en) 2008-07-09 2008-07-09 Method
PCT/IB2009/006379 WO2010004423A2 (en) 2008-07-09 2009-07-08 Method

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US (1) US9321801B2 (da)
EP (1) EP2310520B1 (da)
CN (1) CN102083995A (da)
AR (1) AR072866A1 (da)
AU (1) AU2009269689B2 (da)
BR (1) BRPI0914135A8 (da)
CA (1) CA2732246A1 (da)
DK (1) DK2310520T3 (da)
EA (1) EA201100139A1 (da)
ES (1) ES2615271T3 (da)
GB (1) GB0812559D0 (da)
WO (1) WO2010004423A2 (da)
ZA (1) ZA201008728B (da)

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Publication number Priority date Publication date Assignee Title
WO2010102952A1 (en) * 2009-03-09 2010-09-16 Novozymes A/S Enzymatic removal of steryl glycosides in fatty acid alkyl esters
DE102010055159A1 (de) * 2010-12-18 2012-06-21 Lurgi Gmbh Verfahren zur enzymatischen Reinigung von Ölen pflanzlicher oder tierischer Herkunft
AR091994A1 (es) * 2012-03-16 2015-03-18 Keclon S A Metodo de remocion de esteril glicosidos
US9957453B2 (en) 2013-08-16 2018-05-01 Eth Zurich Enzymatic hydrolysis of acylated steryl glycosides and method for treating biofuel
EP3087163B1 (en) * 2013-12-26 2019-01-30 ExxonMobil Research and Engineering Company Methods of inhibiting precipitation of biodiesel fuel components
EP3404082A1 (de) 2017-05-19 2018-11-21 GEA Mechanical Equipment GmbH Verfahren zur verringerung des gehalts an monoglyceriden (mg), insbesondere an gesättigten monoglyceriden (gmg), in einem roh-biodiesel

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US6827749B2 (en) * 1999-07-07 2004-12-07 The Lubrizol Corporation Continuous process for making an aqueous hydrocarbon fuel emulsions
DE10353150A1 (de) * 2003-11-14 2005-06-16 Cognis Deutschland Gmbh & Co. Kg Verfahren zur gleichzeitigen Herstellung von Sterolen und polaren Lipiden
NZ547082A (en) 2003-12-24 2009-07-31 Danisco Enzymatic treatment of oils
CN101052702B (zh) * 2004-07-16 2013-01-09 杜邦营养生物科学有限公司 脂肪分解酶及其在食品工业中的应用
US20070151146A1 (en) * 2005-12-29 2007-07-05 Inmok Lee Processes of Producing Biodiesel and Biodiesel Produced Therefrom
EP2098585A1 (de) * 2008-02-28 2009-09-09 Süd-Chemie Ag Verfahren zur Reinigung von Biodiesel oder Biodiesel-Vorstufen

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EA201100139A1 (ru) 2011-12-30
GB0812559D0 (en) 2008-08-13
ES2615271T3 (es) 2017-06-06
AU2009269689A1 (en) 2010-01-14
US9321801B2 (en) 2016-04-26
EP2310520A2 (en) 2011-04-20
ZA201008728B (en) 2011-07-27
WO2010004423A2 (en) 2010-01-14
CN102083995A (zh) 2011-06-01
BRPI0914135A2 (pt) 2015-08-25
US20110173876A1 (en) 2011-07-21
WO2010004423A3 (en) 2010-04-22
AR072866A1 (es) 2010-09-29
AU2009269689B2 (en) 2014-11-27
EP2310520B1 (en) 2016-11-23
BRPI0914135A8 (pt) 2016-02-10
CA2732246A1 (en) 2010-01-14

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