DK2336370T3 - Systemer til ekspression af rekombinant Newcastle disease-virus-RNA og vacciner - Google Patents
Systemer til ekspression af rekombinant Newcastle disease-virus-RNA og vacciner Download PDFInfo
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- DK2336370T3 DK2336370T3 DK10012455.1T DK10012455T DK2336370T3 DK 2336370 T3 DK2336370 T3 DK 2336370T3 DK 10012455 T DK10012455 T DK 10012455T DK 2336370 T3 DK2336370 T3 DK 2336370T3
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Claims (22)
1. Rekombinant RNA-molekyle omfattende en heterolog RNA-sekvens, der er flankeret af de 3'- og 5'-ikke-kodende regioner af et Newcastle disease-virus (NDV)-RNA-genom, hvilke regioner indeholder et bindingssted, der er specifikt for en RNA-polymerase af en Newcastle disease-virus, og signaler, der er påkrævet for NDV-medieret replikation og transkription, hvor den heterologe RNA-sekvens er heterolog for NDV-RNA-genomet.
2. Rekombinant RNA-molekyle ifølge krav 1, som er et hybridmolekyle, i hvilket de 3'- og 5'-ikke-kodende regioner er ligerede til den heterologe RNA-sekvens.
3. Rekombinant RNA-molekyle ifølge krav 1, hvor den heterologe RNA-sekvens er insereret i en NDV-genkodende sekvens, således at, når et kimært genprodukt eksprimeres, indeholder det kimære genprodukt den heterologe peptidsekvens i NDV-virusproteinet.
4. Rekombinant RNA-molekyle ifølge krav 1, hvor den heterologe RNA-sekvens fuldstændigt eller delvist erstatter et NDV-gens viruskodende region.
5. Rekombinant RNA-molekyle ifølge et hvilket som helst af kravene 1 eller 3, hvor den heterologe RNA-sekvens er insereret i den HN-, P-, NP-, M, F- eller L-genkodende sekvens.
6. Rekombinant RNA-molekyle ifølge krav 5, hvor den heterologe RNA-sekvens er insereret i HN- eller F-genkodningssekvensen.
7. Rekombinant RNA-molekyle ifølge et hvilket som helst af kravene 1 til 6, hvor den heterologe sekvens er et gen af en anden virus, et gen af et patogen, et cellulært gen eller et antigen, såsom et antigen, der er karakteristisk for et patogen, en autoimmun sygdom, et allergen eller en tumor.
8. Rekombinant RNA-molekyle ifølge krav 7, hvor den heterologe sekvens er et antigen, der er karakteristisk for en autoimmun sygdom eller en tumor.
9. Rekombinant RNA-molekyle ifølge krav 7, hvor den heterologe sekvens er et glycoprotein, fortrinsvis et influenza-glycoprotein eller et glycoprotein af herpesvirus, Epstein-virus, human papillomavirus eller respiratorisk syncyti-alvirus.
10. Rekombinant RNA-molekyle ifølge krav 7, i hvilket genet af en anden virus stammer fra human immundefektvirus, influenzavirus, respiratorisk syn-cytialvirus, Mareks syge-virus, Gumboro-syge-virus, infektiøs bronchitis-virus, infektiøs bursitis-virus, kyllingeanæmivirus, infektiøs laryngotracheitis-virus, aviær leukæmi-virus, retikuloendoteliosevirus, fugleinfluenzavirus, rabiesvirus, kattesygevirus, vesikulær stomatitisvirus, kvægpestvirus eller svinekop-pervirus.
11. Rekombinant RNA-molekyle ifølge et hvilket som helst af kravene 1 til 10, hvor det rekombinante RNA-molekyle har negativ polaritet.
12. Rekombinant RNA-molekyle ifølge et hvilket som helst af kravene 1 til 10, hvor det rekombinante RNA-molekyle har positiv polaritet.
13. Rekombinant celle omfattende nukleotidsekvenser, der koder for et rekombinant RNA-molekyle ifølge et hvilket som helst af kravene 1 til 12 og NDV-RNA-polymeraseproteiner P og L.
14. Kimær negativstrenget RNA-virus omfattende det rekombinante RNA-molekyle ifølge et hvilket som helst af kravene 1 til 11.
15. Fremgangsmåde til fremstilling af en kimær negativstrenget RNA-virus, omfattende at transficere en værtscelle med nukleotidsekvenser, der koder for det rekombinante RNA-molekyle ifølge et hvilket som helst af kravene 1 til 12 og de virale funktioner, der er påkrævet for replikation og transkription, og at udvinde den kimære virus fra kulturen.
16. Vaccineformulering, der omfatter en genmodificeret kimær negativstrenget RNA-virus ifølge krav 14, hvis genom koder for en heterolog epitop.
17. Vaccineformulering ifølge krav 16, i hvilken den heterologe epitop er et immunpotenserende protein.
18. Vaccineformulering ifølge krav 16 eller krav 17 til anvendelse som et lægemiddel.
19. DNA-molekyle, der koder for det rekombinante RNA-molekyle ifølge et hvilket som helst af kravene 1 til 12.
20. Kimært negativstrenget RNA-virus genereret ved anvendelse af omvendt genetik, hvilken virus omfatter et rekombinant RNA-molekyle omfattende en RNA-sekvens, der er flankeret af de 3'- og 5'-ikke-kodende regioner af et NDV-genom, hvilke regioner indeholder et bindingssted, der er specifikt for en RNA-polymerase af en Newcastle disease-virus, og signaler, der er påkrævet for NDV-medieret replikation og transkription.
21. DNA-molekyle, der koder for et rekombinant RNA-molekyle omfattende en RNA-sekvens, der er flankeret af de 3'- og 5'-ikke-kodende regioner af et NDV-genom, hvilke regioner indeholder et bindingssted, der er specifikt for en RNA-polymerase af en NDV, og signaler, der er påkrævet for NDV-medieret replikation og transkription.
22. Fremgangsmåde til fremstilling af et rekombinant RNA-molekyle, omfattende at transficere en værtscelle med DNA-molekylet ifølge krav 21 og de virale funktioner, der er påkrævet for replikation og transkription, og at udvinde det rekombinante RNA-molekyle fra kulturen.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/152,845 US6146642A (en) | 1998-09-14 | 1998-09-14 | Recombinant new castle disease virus RNA expression systems and vaccines |
| EP09015510A EP2213752A1 (en) | 1998-09-14 | 1999-09-14 | Recombinant Newcastle disease virus RNA expression systems and vaccines |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2336370T3 true DK2336370T3 (da) | 2016-02-15 |
Family
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK99948214.4T DK1114189T3 (da) | 1998-09-14 | 1999-09-14 | Rekombinante Newcastle disease virus RNA ekspressionssystemer og vacciner |
| DK10012455.1T DK2336370T3 (da) | 1998-09-14 | 1999-09-14 | Systemer til ekspression af rekombinant Newcastle disease-virus-RNA og vacciner |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
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| DK99948214.4T DK1114189T3 (da) | 1998-09-14 | 1999-09-14 | Rekombinante Newcastle disease virus RNA ekspressionssystemer og vacciner |
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| AT (1) | ATE452210T1 (da) |
| AU (1) | AU772649B2 (da) |
| BR (1) | BRPI9913721B8 (da) |
| CA (1) | CA2343653C (da) |
| CY (1) | CY1110704T1 (da) |
| DE (1) | DE69941820D1 (da) |
| DK (2) | DK1114189T3 (da) |
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