DK2370112T3 - Implanterbar liposomindlejret matrixsammensætning og anvendelser deraf - Google Patents

Implanterbar liposomindlejret matrixsammensætning og anvendelser deraf Download PDF

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DK2370112T3
DK2370112T3 DK09828393.0T DK09828393T DK2370112T3 DK 2370112 T3 DK2370112 T3 DK 2370112T3 DK 09828393 T DK09828393 T DK 09828393T DK 2370112 T3 DK2370112 T3 DK 2370112T3
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matrix
liposomes
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monocytes
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Shai Yehoshua Schubert
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Bonus Cellora Ltd
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    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
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    • A61L27/3804Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
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    • A61L27/40Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
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Claims (23)

  1. IMPLANTERE AR LIPOSOMINDLEJRET MATRIXS AMMEN SÆTNIN G OG ANVENDELSER DERAF
    1. Implanterbar, halvfast matrix omfattende et hydrogelmateriale og liposomer indlejret deri, hvori liposomeme indeholder mindst ét biologisk aktivt materiale, og hvori matrixen er permeabeS for infiltration af cel ler fra et implantationsmiljø og for produkter udskilt fra cellerne til miljøet, hvori det biologisk aktive materiale er udvalgt fra gruppen bestående af et protein, et peptid, et nukleotid, et DMA, et RNA, et siRNA og et cDNA, eller en kombination deraf, eller er et lille molekyle, et lægemiddel eller en kombination deraf; og hvori matrixen inkluderer en eller flere kemoattraktanter for monocytiske celler; og hvori liposomeme har en størrelse i området 25 til 400 nm.
  2. 2. Matrix ifølge krav 1, hvori det mindst ene biologisk aktive materiale er i stand til at fremkalde en angiogen fænotype i infiltrerende celler, der fagocyterer liposomeme efter implantation inden i et subjekt.
  3. 3. Matrix ifølge krav 1 eller 2, hvori liposomeme bærer det biologisk aktive materiale i den hydrofile liposomkeme, i membranen, på liposomoverfladen eller i en kombination deraf.
  4. 4. Matrix ifølge et hvilket som helst af kravene 1-3, hvori kemoattraktanten er monoeyt-kemotaktisk protein-1 eller granulocyt-makrofagkolonistimulerende faktor (GM-CSF).
  5. 5. Matrix ifølge et hvilket som helst af kravene 1-4, hvori liposomeme er forbundet med hydrogelmatrixen med en kemisk binding udvalgt fra gruppen bestående af en peptidbinding eller en ionbinding.
  6. 6. Matrix ifølge et hvilket søm helst af kravene 1-5, hvori hydrogelmateriaiet indeholder celler tilsat til hydrogelmateriaiet, før dets implantation i kroppen.
  7. 7. Matrix ifølge et hvilket som helst af de foregående krav, hvori hydrogelen omfatter polyhydroxysyrer, polyortoestere, polyanhydrider, gelatine, kollagen, cellulose, chitosan, aiginat, hyaluronan, thiol-modificeret hyaluronan og kombinationer eller copolymerer deraf.
  8. 8. Matrix ifølge et hvilket som helst af de foregående krav endvidere omfattende et tværbindingsmiddel.
  9. 9. Matrix ifølge et hvilket som helst af de foregående krav, hvori det biologisk aktive materiale indeholdt i liposomeme er et tumorantigen, eller hvori det biologisk aktive materiale indeholdt i liposomerne er et nukiemsyreniolekyle, der koder for et tumorantigen.
  10. 10. Matrix ifølge et hvilket som helst af kravene 1-8, hvori det biologisk aktive materiale, der er indeholdt i liposomerne, er et middel, der fremmer differentiering af monocytiske celler til dendritiske celler, udvalgt fra gruppen bestående af granulocyt-makrofagkolonistirnulerende faktor (GM-CSF), interleukin-4, TNF-alfa og interleukin-15 og kombinationer deraf.
  11. 11. Matrix ifølge et hvilket som helst af kravene 1 -8, hvori det biologisk aktive materiale er en ekspressionsvektor, der koder for en transskriptionsfaktor.
  12. 12. Matrix ifølge krav 11, hvori transskriptionsfaktoren er i stand til at differentiere cellen til en insulinproducerende celle, eller hvori transskriptionsfaktoren er udvalgt fra gruppen bestående afNGN-3, PDX- 1, MAFA, Oct4, Sox2, c-Myc, klf-4, Nanog, Polycomb-gruppe-(PcG)-proteiner, PAX6, MEIS1, QTX1, ATBF1, DLX5, HANDI, Nuklear faktor kappa-B og OENCUT1.
  13. 13. Matrix ifølge et hvilket som helst af de foregående krav, hvori det biologisk aktive materiale, der er indeholdt i liposomeme, er udvalgt fra gruppen bestående af adenosin, IL-4, IL-10, IL-13, en IL-1-receptorligand, PGE2, TGF-beta, TNF-alfa, mælkesyre, lipoteichoinsyre, NADH-dehydrogenase 5, subunit 1, poly-(adenosindiphosphat-ribose)-polymerase, pyruvat, kolonistimulerende faktor-1, NECA, EPS, Pam3CSK4, E. coli LPS, R848, imiquimod, ikkc-mcthylcrct CpG-DNA, ODN2006, thiorcdoxin-peroxidase, Trapidil, en TLR2-agonist, en TLR3-agonist, en TLR4-agonist, en TLR7-agonist, en TLR8-agonist, en TLR9-agonist, adenosin Al-agonister, adenosin A2-agonister, adenosin A3-agonistser, alendronat, et polypeptid-tumorantigen afledt af tumorceller eller tumorvæv og kombinationer deraf.
  14. 14. Matrix ifølge et hvilket som helst af kravene 1-13 til anvendelse i modulering af en monocytisk celles adfærd på eller i nærheden af implantationsstedet hos et subjekt.
  15. 15. Matrix ifølge krav 14, hvori adfærden er en angiogen aktivitet, en immunsuppressiv aktivitet, en inflammatorisk aktivitet, en antigen-præsenterende aktivitet, celledifferentiering eller celle-omprogrammering.
  16. 16. Matrix ifølge krav 34 eller 15, hvori cellen er en monocyt.
  17. 17. Matrix ifølge et hvilket søm helst af kravene 1-13 til anvendelse i til førsel af et udskilt produkt til et lokaliseret sted inden i et iskæmisk væv hos et subjekt.
  18. 18. Fremgangsmåde til programmering af den implanterbare matrix ifølge krav 1 til at modulere en infiltrerende monocytisk celles adfærd, omfattende formulering inden i liposomerne af et eller flere biologisk aktive materialer, der er i stand til at modulere cellens adfærd, og indlejring af liposomerne i en matrix, der inkluderer et eller flere midler til rekruttering af celler til matrixen.
  19. 19. Matrix ifølge et hvilket som helst af kravene 1-13 til anvendelse i præsentation af et tumorantigen, hvori liposomerne omfatter et tumorantigen.
  20. 20. Matrix ifølge et hvilket som helst af kravene 14-16, hvori adfærden er differentiering til insulinproducerende celler eller differentiering til dopaminproducerende celler.
  21. 21. Fremgangsmåde ifølge krav 18, hvori implantationsstedet er inden i pancreas.
  22. 22. Matrix ifølge et hvilket som helst af kravene 1-13 til anvendelse til fremme af insulinudskillelse på et lokaliseret sted hos et subjekt med insulinresistens eller insulinmangel, hvori liposomerne omfatter en ekspression s vektor, der koder for en transskriptionsfaktor, der er i stand til at differentiere en celle til en insulinproducerende celle.
  23. 23. Matrix ifølge et hvilket som helst af kravene 1-22, hvori liposomerne er sterisk beskyttede, kolcstcrol-modificcrcdc, PEGylcrcdc eller ovcrfladcmodificcrcdc.
DK09828393.0T 2008-11-24 2009-11-24 Implanterbar liposomindlejret matrixsammensætning og anvendelser deraf DK2370112T3 (da)

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US20021308P 2008-11-24 2008-11-24
US20020708P 2008-11-24 2008-11-24
PCT/US2009/065797 WO2010060104A2 (en) 2008-11-24 2009-11-24 Implantable liposome embedded matrix composition, uses thereof, and polycaprolactone praticles as scaffolds for tissue regeneration

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