DK2374876T3 - Fremgangsmåde til oprensning af alfa-galactosidase A - Google Patents
Fremgangsmåde til oprensning af alfa-galactosidase A Download PDFInfo
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- DK2374876T3 DK2374876T3 DK10181859.9T DK10181859T DK2374876T3 DK 2374876 T3 DK2374876 T3 DK 2374876T3 DK 10181859 T DK10181859 T DK 10181859T DK 2374876 T3 DK2374876 T3 DK 2374876T3
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
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Claims (9)
1. Fremgangsmåde til oprensning af humant α-galactosidase A (α-Gal A) fra en prøve, omfattende at lade prøven passere over en hydrofob interaktionsresin omfattende en butylgruppe som en funktionel enhed.
2. Fremgangsmåden ifølge krav 1, hvor prøven der lades passere over en hydrofob interaktionsresin omfattende en butylgruppe som en funktionel enhed er det første kromatografitrin af fremgangsmåden.
3. Fremgangsmåden ifølge krav 1 eller 2, hvor fremgangsmåden omfatter at lade prøven passere over en hydrofob interaktionsresin og efterfølgende over en immobiliseret heparinresin, en hydroxyapatitsøjle, en anionbytterresin, eller en størrelseseksklusionsresin.
4. Fremgangsmåden ifølge krav 1 eller 2, hvor fremgangsmåden omfatter at lade prøven passere over en hydrofob interaktionsresin og efterfølgende over en immobiliseret heparinresin, en hydroxyapatitsøjle, en anionbytterresin, og en størrelseseksklusionsresin
5. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 4, hvor prøven opnås ved at dyrke en human celle der er blevet genetisk modificeret til at overudtrykke humant α-Gal A under betingelser der tillader overekspression og sekretion af humant α-Gal A og isolere humant α-Gal A fra de dyrkede-celler ved at samle mediet i hvilket-cellerne vokser og/eller ved at lysere cellerne til at frigive deres indhold.
6. Fremgangsmåden ifølge krav 5, hvor de humane celler vælges fra gruppen bestående af en fibroblastcelle, en epitelcelle, en endotelcelle, en lymfocyt, en knoglemarvcelle, en gliacelle, en hepatocyt, en keratinocyt, en muskelcelle, en nervecelle, eller en prækursor af en hvilken som helst af disse celletyper.
7. Fremgangsmåden ifølge krav 5, hvor den humane celle er fra en immortaliseret cellelinje valgt fra gruppen bestående afen Bowes-melanomcelle, en Daudi-celle, en HeLa-celle, en HL-60-celle, en HT1080-celle, en Jurkat-celle, en KB-carcinoma- celle, en K-562-leukæmi-celle, en MCF-7-brystcancer-celle, en MOLT-4-celle, en Namalwa-celle, en Raji-celle, en RPMI 8226-celle, en U-937-celle, en WI-38VA13-underlinje 2R4-celle, og en 2780AD-ovarie-carcinoma-celle.
8. Fremgangsmåden ifølge et hvilket som helst af kravene 5-7, hvor den dyrkede human celle er blevet genetisk modificeret til at indeholde et DNA-molekyle der koder for humant α-Gal A-bundet ved N-terminus til signalpeptidet af humant væksthormon (hGH) med aminosyresekvensen SEQ ID NO:21.
9. Fremgangsmåden ifølge krav 8, hvor humant α-Gal A uden signalpeptidet har aminosyresekvensen SEQ ID NO:26.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
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| US71261496A | 1996-09-13 | 1996-09-13 | |
| EP04030107.9A EP1538202B1 (en) | 1996-09-13 | 1997-09-12 | Production of human alpha-galactosidase A |
Publications (1)
| Publication Number | Publication Date |
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| DK2374876T3 true DK2374876T3 (da) | 2016-05-30 |
Family
ID=24862863
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| Application Number | Title | Priority Date | Filing Date |
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| DK10181859.9T DK2374876T3 (da) | 1996-09-13 | 1997-09-12 | Fremgangsmåde til oprensning af alfa-galactosidase A |
| DK04030107.9T DK1538202T3 (da) | 1996-09-13 | 1997-09-12 | Fremstilling af humant alpha-galactosidase a |
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| Application Number | Title | Priority Date | Filing Date |
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| DK04030107.9T DK1538202T3 (da) | 1996-09-13 | 1997-09-12 | Fremstilling af humant alpha-galactosidase a |
Country Status (20)
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| US6274597B1 (en) | 1998-06-01 | 2001-08-14 | Mount Sinai School Of Medicine Of New York University | Method of enhancing lysosomal α-Galactosidase A |
| AU2012241170B2 (en) * | 1999-03-11 | 2016-07-28 | Shire Human Genetic Therapies, Inc. | Treatment of alpha-Galactosidase A deficiency |
| AU2016250357B2 (en) * | 1999-03-11 | 2018-11-01 | Shire Human Genetic Therapies, Inc. | Treatment of alpha-Galactosidase A deficiency |
| AU2004242550B2 (en) * | 1999-03-11 | 2008-04-03 | Shire Human Genetic Therapies, Inc. | Treatment of alpha-Galactosidase A deficiency |
| EP2275559A3 (en) * | 1999-09-28 | 2011-03-23 | Shire Human Genetic Therapies, Inc. | Optimized messenger RNA |
| WO2002064799A2 (en) | 1999-09-28 | 2002-08-22 | Transkaryotic Therapies, Inc. | Optimized messenger rna |
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| DE60138535D1 (de) | 2000-02-04 | 2009-06-10 | Children S Hospital Res Founda | Verwendung von lysosomal acid lipase zur behandlung von atherosklerose und ähnlichen krankheiten |
| US20020095135A1 (en) | 2000-06-19 | 2002-07-18 | David Meeker | Combination enzyme replacement, gene therapy and small molecule therapy for lysosomal storage diseases |
| US7138262B1 (en) * | 2000-08-18 | 2006-11-21 | Shire Human Genetic Therapies, Inc. | High mannose proteins and methods of making high mannose proteins |
| IL140110A0 (en) | 2000-12-05 | 2002-02-10 | Applied Research Systems | Improvement of homogeneity and secretion of recombinant proteins in mammalian systems |
| DK1503788T3 (da) | 2002-04-25 | 2011-10-17 | Shire Human Genetic Therapies | Behandling af alpha-galactosidase A-deficiens |
| PT1587923E (pt) | 2003-01-22 | 2011-12-07 | Univ Duke | Construções melhoradas para a expressão de polipéptidos lisossomais |
| JP4368925B2 (ja) | 2005-11-18 | 2009-11-18 | 財団法人 東京都医学研究機構 | 基質特異性を変換した新規高機能酵素 |
| AR059089A1 (es) * | 2006-01-20 | 2008-03-12 | Genzyme Corp | Administracion intraventricular de una enzima para enfermedades de almacenamiento lisosomal |
| ES2391657T3 (es) | 2006-02-07 | 2012-11-28 | Shire Human Genetic Therapies, Inc. | Composiciones estabilizadas de proteínas que tienen un resto tiol libre |
| CN101404927A (zh) | 2006-02-09 | 2009-04-08 | 建新公司 | 慢速脑室内递送 |
| US8569032B2 (en) | 2007-05-18 | 2013-10-29 | Tokyo Metropolitan Institute Of Medical Science | Proteins having acquired A-galactosidase activity |
| EP3608330B1 (en) | 2008-12-16 | 2022-11-09 | Genzyme Corporation | Synthetic intermediates for preparing oligosaccharide-protein conjugates |
| AU2010281403B2 (en) | 2009-07-28 | 2016-03-10 | Takeda Pharmaceutical Company Limited | Compositions and methods for treating Gaucher disease |
| KR101742346B1 (ko) | 2010-04-23 | 2017-06-15 | 시나게바 바이오파르마, 코포레이션 | 리소솜 축적병 효소 |
| WO2012012461A2 (en) | 2010-07-19 | 2012-01-26 | Shire Human Genetic Therapies, Inc. | Mannose receptor c type 1 (mrc1) codon optimized cell line and uses thereof |
| BR122019021757B1 (pt) | 2010-09-09 | 2020-12-08 | Alexion Pharmaceuticals, Inc | composição farmacêutica para tratamento de um paciente humano sofrendo de uma deficiência de lipase ácida lisossomal (lal) |
| EP2675472A4 (en) | 2011-02-15 | 2014-09-17 | Synageva Biopharma Corp | METHOD FOR THE TREATMENT OF LACK OF LYSOSOMAL SAURER LIPASE |
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| WO2013130963A1 (en) | 2012-03-02 | 2013-09-06 | Shire Human Genetic Therapies, Inc. | Compositions and methods for treating type iii gaucher disease |
| BR112014032919B1 (pt) * | 2012-06-29 | 2023-03-28 | Universitat De Barcelona | Conjugado e seu método de produção, lipossomas, uso e método de produção dos mesmos, bem como composição farmacêutica |
| WO2014016873A1 (en) | 2012-07-26 | 2014-01-30 | Jcr Pharmaceuticals Co., Ltd. | Method for production of recombinant human alpha-galactosidase a |
| MX379269B (es) | 2012-10-24 | 2025-03-11 | Genzyme Corp | Elucion de biomoleculas de resinas multimodales utilizando mes y mops como modificadores de fase movil. |
| TWI793159B (zh) | 2013-10-23 | 2023-02-21 | 美商健臻公司 | 重組醣蛋白及其用途 |
| CA2970638A1 (en) | 2014-12-22 | 2016-06-30 | Codexis, Inc. | Human alpha-galactosidase variants |
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| WO2019004446A1 (ja) | 2017-06-30 | 2019-01-03 | 富士フイルム株式会社 | ライソゾーム病処置剤 |
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| MY207075A (en) | 2018-12-20 | 2025-01-28 | Codexis Inc | Human alpha-galactosidase variants |
| CN111308095A (zh) * | 2020-03-04 | 2020-06-19 | 北京师范大学 | 用于诊断前列腺癌的尿液蛋白标记物 |
| TW202423959A (zh) * | 2022-08-24 | 2024-06-16 | 美商步行魚醫療公司 | 用於治療法布瑞氏症(fabry disease)之組成物及方法 |
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| JPH06503479A (ja) * | 1991-10-16 | 1994-04-21 | オラージェン， インコーポレイテッド | 動物体細胞の粒子−媒介トランスフォーメーション |
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1999
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-
2009
- 2009-02-06 NO NO20090583A patent/NO330687B1/no not_active IP Right Cessation
-
2011
- 2011-03-31 NO NO20110496A patent/NO340408B1/no not_active IP Right Cessation
- 2011-09-06 JP JP2011193635A patent/JP2012019793A/ja not_active Withdrawn
-
2014
- 2014-09-29 NO NO20141168A patent/NO20141168L/no not_active Application Discontinuation
- 2014-10-15 JP JP2014210566A patent/JP2015044830A/ja active Pending
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