DK2396403T3 - Kold-aktiv beta-galactosidase, fremgangsmåde til fremstilling heraf og anvendelse af et sådant enzym - Google Patents

Kold-aktiv beta-galactosidase, fremgangsmåde til fremstilling heraf og anvendelse af et sådant enzym Download PDF

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Publication number
DK2396403T3
DK2396403T3 DK10703071.0T DK10703071T DK2396403T3 DK 2396403 T3 DK2396403 T3 DK 2396403T3 DK 10703071 T DK10703071 T DK 10703071T DK 2396403 T3 DK2396403 T3 DK 2396403T3
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Denmark
Prior art keywords
beta
galactosidase
lactose
seq
leu
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DK10703071.0T
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English (en)
Inventor
Peter Stougaard
Mariane Schmidt
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Coldzymes Aps
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Publication of DK2396403T3 publication Critical patent/DK2396403T3/da

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1206—Lactose hydrolysing enzymes, e.g. lactase, beta-galactosidase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (16)

  1. KOLD-AKTIV BETA-GALACTOSIDASE, FREMGANGSMÅDE TIL FREMSTILLING HERAF OG ANVENDELSE AF ET SÅDANT ENZYM
    1. Oprenset kold-aktiv beta-galactosidase, der har aminosyresekvensen som defineret i SEQ ID NO: 1 eller har én mindst 80 % homologi med aminosyresekvensen som defineret i SEQ ID NO: 1, hvilken aminosyresekvens er udvalgt, således at enzymet har en stabil enzymatisk aktivitet ved temperaturer på mindre end 8 °C.
  2. 2. Beta-galactosidase ifølge krav 1, hvor aminosyresekvensen har mindst 90 %, fortrinsvis 95 %, homologi med aminosyresekvensen som defineret i SEQ ID NO: 1.
  3. 3. Beta-galactosidase ifølge krav 1 eller 2, hvor den er fremstillet af en stamme af en Alkalilactibacillus.
  4. 4. Beta-galactosidase ifølge krav 1,2 eller 3, hvor den er fremstillet af Alkalilactibacillus ikkense.
  5. 5. Isoleret DNA-sekvens omfattende et gen, der koder for beta-galactosidasen ifølge et hvilket som helst af kravene 1 til 4.
  6. 6. Isoleret DNA-sekvens, der a) koder for et protein med en aminosyresekvens som i SEQ ID NO: 2, eller b) hybridiserer under stringente betingelser til sekvensen af a), eller c) er degenerativ af sekvensen af a) eller b).
  7. 7. DNA-sekvens ifølge krav 6, hvor sekvensen er som i SEQ ID NO: 2.
  8. 8. Rekombinant vektor omfattende en DNA-sekvens ifølge et hvilket som helst af kravene 5 til 7.
  9. 9. Vektor ifølge krav 8, hvor vektoren er en ekspressionsvektor.
  10. 10. Værtscelle transformeret med en vektor ifølge krav 8 eller 9.
  11. 11. Celle ifølge krav 10, hvor cellen er udvalgt fra gruppen bestående af Escherichia, Bacillus, Bifidobacterium, Lactococcus, Lactobacillus, Streptomyces, Leuconostoc, Streptomyces, Saccharomyces, Kluyveromyces, Candida, Torula, Torulopsis og Aspergillus.
  12. 12. Celle ifølge krav 11, hvor cellen er udvalgt fra gruppen bestående af Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium infantis, Bifidobacterium bifidum, Bifidobacterium animalis og Lactococcus lactis.
  13. 13. Anvendelse af en celle ifølge et hvilket som helst af kravene 10 til 12 til fremstilling af et produkt udvalgt fra gruppen bestående af lactosefri mælk, lavlactosemælk, yoghurt, ost, fermenterede mælkeprodukter, kosttilskud og probiotisk spiseligt produkt.
  14. 14. Anvendelse ifølge krav 13 til fremstilling af et mejeriprodukt med en lactosekoncentration på 1 % eller lavere.
  15. 15. Anvendelse ifølge krav 14, hvor lactosekoncentrationen er 0,1 % eller lavere.
  16. 16. Psykrofil bakterie Alkalilactibacillus ikkense BCCM-accessionsnr. LMG P-24866 og varianter og mutanter afledt deraf, der er i stand til at fremstille en kold-aktiv beta-galactosidase defineret i krav 1 eller 2.
DK10703071.0T 2009-02-10 2010-02-09 Kold-aktiv beta-galactosidase, fremgangsmåde til fremstilling heraf og anvendelse af et sådant enzym DK2396403T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15120809P 2009-02-10 2009-02-10
US17695609P 2009-05-11 2009-05-11
PCT/EP2010/051596 WO2010092057A1 (en) 2009-02-10 2010-02-09 Cold-active beta-galactosidase, a method of producing same and use of such enzyme

Publications (1)

Publication Number Publication Date
DK2396403T3 true DK2396403T3 (da) 2016-01-11

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DK10703071.0T DK2396403T3 (da) 2009-02-10 2010-02-09 Kold-aktiv beta-galactosidase, fremgangsmåde til fremstilling heraf og anvendelse af et sådant enzym

Country Status (6)

Country Link
US (1) US8288143B2 (da)
EP (1) EP2396403B8 (da)
CN (1) CN102361974B (da)
CA (1) CA2751957C (da)
DK (1) DK2396403T3 (da)
WO (1) WO2010092057A1 (da)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102220301B (zh) * 2011-05-30 2012-10-24 云南师范大学 一种耐碱性低温α-半乳糖苷酶AgaAJB13及基因
EP2530148B1 (en) 2011-05-30 2015-04-29 B.R.A.I.N. Biotechnology Research And Information Network AG Novel beta-galactosidases useful for the production of lactose depleted milk products
KR101619863B1 (ko) * 2014-05-23 2016-05-24 주식회사 제노포커스 신규 베타-갈락토시다아제
BR112017011607A2 (pt) 2014-12-05 2018-01-09 Godo Shusei Kk solução de lactase e produto lácteo usando a mesma
CN110678479A (zh) 2017-04-11 2020-01-10 科·汉森有限公司 性能改善的乳糖酶
ES3057273T3 (en) 2017-04-11 2026-02-27 Kerry Group Services Int Ltd Lactase enzymes with improved properties
US12435326B2 (en) * 2017-04-11 2025-10-07 Kerry Group Services International Ltd Lactase enzymes with improved properties
FI3609909T3 (fi) 2017-04-11 2025-09-03 Kerry Group Services Int Ltd Laktaasientsyymit, joilla on parannettu aktiivisuus matalissa lämpötiloissa
EP3778625A4 (en) * 2018-04-05 2022-02-09 Godo Shusei Co., Ltd. ENZYME DERIVED FROM PAENIBACILLUS PABULI FOR THE PRODUCTION OF GALACTOOLIGOSACCHARIDE AND PROCESS FOR THE PRODUCTION OF GALACTOOLIGOSACCHARIDE
BR112021007244A2 (pt) * 2018-10-17 2021-08-10 Chr. Hansen A/S enzimas lactase com propriedades melhoradas em ph ácido
WO2021037986A1 (en) 2019-08-30 2021-03-04 Chr. Hansen A/S Production of lactase enzymes using altered regulation strains
CN112626052B (zh) * 2020-12-09 2021-11-19 山东隆科特酶制剂有限公司 一种热稳定性提高的多聚半乳糖醛酸酶突变体及应用
CN113637660B (zh) * 2021-08-05 2023-09-08 云南师范大学 一种β-半乳糖苷酶GalNC3-89及其制备方法和应用
CN113621550B (zh) * 2021-09-23 2022-06-21 武汉瀚海新酶生物科技有限公司 用于发酵产碱性磷酸酶的培养基和碱性磷酸酶的制备方法

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JP2003504048A (ja) * 1999-07-09 2003-02-04 ユニベルシテ ドゥ リエージュ 低温活性ベータ・ガラクトシダーゼ、その製法、及びその使用

Also Published As

Publication number Publication date
CN102361974B (zh) 2013-11-06
EP2396403B8 (en) 2015-11-18
US8288143B2 (en) 2012-10-16
CA2751957C (en) 2018-07-24
US20120058223A1 (en) 2012-03-08
EP2396403B1 (en) 2015-09-23
EP2396403A1 (en) 2011-12-21
CN102361974A (zh) 2012-02-22
CA2751957A1 (en) 2010-08-19
WO2010092057A1 (en) 2010-08-19

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