DK2417153T3 - Filtrering af en væske, der omfatter et stressprotein fra en plante - Google Patents
Filtrering af en væske, der omfatter et stressprotein fra en plante Download PDFInfo
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- DK2417153T3 DK2417153T3 DK10713337.3T DK10713337T DK2417153T3 DK 2417153 T3 DK2417153 T3 DK 2417153T3 DK 10713337 T DK10713337 T DK 10713337T DK 2417153 T3 DK2417153 T3 DK 2417153T3
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- Prior art keywords
- stress protein
- liquid
- filtration
- filter aid
- plant
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- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 206010040560 shock Diseases 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- JVBXVOWTABLYPX-UHFFFAOYSA-L sodium dithionite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])=O JVBXVOWTABLYPX-UHFFFAOYSA-L 0.000 description 1
- CSRCBLMBBOJYEX-UHFFFAOYSA-M sodium;2-morpholin-4-ylethanesulfonic acid;hydroxide Chemical compound [OH-].[Na+].OS(=O)(=O)CCN1CCOCC1 CSRCBLMBBOJYEX-UHFFFAOYSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
Claims (15)
1. Metode til at genindvinde et stressprotein fra en plante, hvor metoden omfatter at indvinde, fra ovennævnte plante, en væske, der omfatter stressproteinet; at udskille, fra ovennævnte væske, en eller flere komponenter ud over stressproteinet; at tilføje en fibrøs filterhjælp og et mineralsk pulver til væsken; derefter udsætte væsken, der omfatter stressproteinet, samt de(n) udskilte komponent(er), den fibrøse filterhjælp og det mineralske pulver, for et første filtreringstrin over et filter, og derved separere den udskilte, fibrøse filterhjælp og det mineralske pulver fra filtratet, der omfatter stressproteinet; derefter udsætte filtratet for et yderligere filtreringstrin; og derefter rense stressproteinet.
2. Metode, ifølge krav 1, hvor, i det første filtreringstrin, klorofyl og/eller rubisco ijemes fra væsken.
3. Metode, ifølge krav 1 eller 2, hvor det mineralske pulver omfatter mindst ét komet mineral.
4. Metode, ifølge krav 3, der omfatter mindst ét komet mineral, udvalgt fra gruppen diatoméjord og perlit.
5. Metode, ifølge et hvilket som helst af ovenstående krav, hvor den fibrøse filterhjælp omfatter cellulosefibre.
6. Metode, ifølge et hvilket som helst af ovenstående krav, hvor den fibrøse filterhjælp er en blanding af fibre med forskellige længder, og hvor blandingen leverer i det mindste fibre med en længde i et omfang, der strækker sig fra cirka 20 pm til cirka 130 μηι.
7. Metode, ifølge et hvilket som helst af ovenstående krav, hvor det yderligere filtreringstrin, som filtratet er udsat for, er et dybdefiltreringstrin.
8. Metode, ifølge et hvilket som helst af ovenstående krav, hvor, efter det yderligere filtreringstrin, en fraktion, der omfatter stressproteinet, er udsat for et væskefjemelsestrin, hvorved stressproteinet koncentreres i fraktionen, helst ved omvendt osmose, ultrafiltrering eller nanofiltrering, og/eller for et saltijcrnclsestrin, helst ved dialyse.
9. Metode, ifølge et hvilket som helst af ovenstående krav, hvor stressproteinet renses ved kromatografi.
10. Metode, ifølge krav 9, hvor kromatografien omfatter et første stærkt anionbytningstrin, derefter et affinitetskromatografi-adskillelsestrin, og derefter et andet stærkt anionbytningstrin.
11. Metode, ifølge krav 10, hvor den gennemsnitlige partikelstørrelse af bytningsmaterialet, der anvendes i den første anionbytning, er større end den gennemsnitlige partikelstørrelse af bytningsmaterialet, der anvendes i den anden anionbytning.
12. Metode, ifølge et hvilket som helst af ovenstående krav, hvor stressproteinet er et stressprotein fra en plante, udvalgt fra gruppen Fabaceae, Chenopodiaceae, Solanaceae og Poaceae, især fra gruppen lucerne, hvede, majs, ris, soja, kartoffel, bede, mere især fra lucerne.
13. Metode, ifølge et hvilket som helst af ovenstående krav, hvor stressproteinet er udvalgt fra gruppen HSP40, HSP60, HSP70 og HSP90.
14. Metode, ifølge et hvilket som helst af ovenstående krav, hvor filtratet fra det første filtreringstrin er udsat for ovennævnte yderligere filtreringstrin uden først at være blevet udsat for centrifugering.
15. Anvendelse af mindst ét komet mineral, udvalgt fra gruppen diatoméjord og perlit, til at fjerne klorofyl og/eller carotenoid fra en væske, især en plantesaft, der indeholder et varmechokprotein.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09157774 | 2009-04-09 | ||
| PCT/EP2010/054723 WO2010115990A1 (en) | 2009-04-09 | 2010-04-09 | Filtration of a liquid comprising a plant stress protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2417153T3 true DK2417153T3 (da) | 2014-12-01 |
Family
ID=40943897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10713337.3T DK2417153T3 (da) | 2009-04-09 | 2010-04-09 | Filtrering af en væske, der omfatter et stressprotein fra en plante |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8933200B2 (da) |
| EP (1) | EP2417153B1 (da) |
| DK (1) | DK2417153T3 (da) |
| ES (1) | ES2524541T3 (da) |
| WO (1) | WO2010115990A1 (da) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10159709B2 (en) | 2012-12-07 | 2018-12-25 | Alfa Biogene International B.V. | HSP for use in treatment for imiquimod related side effects |
| CN109535240B (zh) * | 2018-11-02 | 2022-05-20 | 广东海洋大学 | 一种改善鱼肉肌球蛋白性能的方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3872016A (en) * | 1974-03-25 | 1975-03-18 | Johns Manville | Filter aid materials dispersible in organic liquids |
| DE19804882A1 (de) | 1998-02-09 | 1999-08-12 | Herzog Stefan | Filterhilfsmittel |
| GEP20002333B (en) | 1999-05-19 | 2000-09-10 | Method for Inducing Synthesis of Heat Stress Proteins in Herbal Plants | |
| NL1018211C2 (nl) | 2001-06-05 | 2002-12-10 | Alfa Biogene Internat B V | Het winnen van een stress-eiwit. |
| EP1531160B1 (en) | 2003-11-12 | 2010-12-29 | Alfa Biogene International B.V. | Recovery of a heat shock protein |
| CA2615372A1 (en) | 2007-07-13 | 2009-01-13 | Marc-Andre D'aoust | Influenza virus-like particles (vlps) comprising hemagglutinin |
-
2010
- 2010-04-09 EP EP10713337.3A patent/EP2417153B1/en not_active Not-in-force
- 2010-04-09 DK DK10713337.3T patent/DK2417153T3/da active
- 2010-04-09 ES ES10713337.3T patent/ES2524541T3/es active Active
- 2010-04-09 WO PCT/EP2010/054723 patent/WO2010115990A1/en not_active Ceased
- 2010-04-09 US US13/263,445 patent/US8933200B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010115990A1 (en) | 2010-10-14 |
| EP2417153A1 (en) | 2012-02-15 |
| US8933200B2 (en) | 2015-01-13 |
| EP2417153B1 (en) | 2014-11-05 |
| ES2524541T3 (es) | 2014-12-10 |
| US20120095189A1 (en) | 2012-04-19 |
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