DK2443236T3 - Fremgangsmåder og sammensætninger til sekventering ved lang fragmentlæsning - Google Patents
Fremgangsmåder og sammensætninger til sekventering ved lang fragmentlæsning Download PDFInfo
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- DK2443236T3 DK2443236T3 DK10725582.0T DK10725582T DK2443236T3 DK 2443236 T3 DK2443236 T3 DK 2443236T3 DK 10725582 T DK10725582 T DK 10725582T DK 2443236 T3 DK2443236 T3 DK 2443236T3
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- adaptor
- nucleic acid
- fragments
- dna
- target nucleic
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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Claims (12)
1. Fremgangsmåde til fragmentering af en dobbeltstrenget målnukleinsyre, hvilken fremgangsmåde omfatter: (a) tilvejebringelse af genomisk DNA, (b) opdeling af DNAet i en første række af separate portioner, (c) opformering af DNAet i de separate portioner til frembringelse af et antal amplikoner, hvor opformeringen udføres med en population af dNTPer, hvilken population af dNTPer omfatter: (i) et forudbestemt forhold mellem dUTP og dTTP, således at et antal thyminer i DNAet erstattes med uraciler, (ii) et forudbestemt forhold mellem 5-methyl-dCTP og dCTP, således at et antal cytosiner erstattes med 5-methylcytosiner, (d) fjernelse af uracilerne og 5-methylcytosinerne fra amplikonerne til frembringelse af gap-holdigt DNA, (e) behandling af det gap-holdige DNA til translation af gaps, indtil gaps på modsatte strenge løber sammen til derved frembringelse af stump-endede DNA-fragmenter.
2. Fremgangsmåde ifølge krav 1, hvor fremgangsmåden yderligere omfatter opnåelse af et antal sekvenslæsninger fra fragmenter fra hver separate portion i den første række.
3. Fremgangsmåde ifølge krav 1-2, hvor fremgangsmåden yderligere omfatter opdeling af fragmenterne i en anden række af separate portioner.
4. Fremgangsmåde ifølge krav 3, hvor fremgangsmåden yderligere omfatter opnåelse af et antal sekvenslæsninger fra fragmenter fra hver separate portion i den anden række.
5. Fremgangsmåde ifølge krav 1-4, hvor de separate portioner har et volumen på mindre end 1 yL, 100 nL, 10 nL, 1 nL eller 100 pL.
6. Fremgangsmåde ifølge krav 1-5, hvor opformeringstrinet (b) udføres i nærvær af et element, der er udvalgt blandt glycogen, DMSO, ET SSB, betain og en hvilken som helst kombination deraf.
7. Fremgangsmåde ifølge krav 1-6, hvor de stump-endede DNA-fragmenter har længder på ca. 100 kb til ca. 1 mb.
8. Fremgangsmåde til fragmentering af en dobbeltstrenget målnukleinsyre, hvilken fremgangsmåde omfatter: (a) tilvejebringelse af genomisk DNA, (b) opdeling af DNAet i separate portioner, (c) opformering af DNAet i de separate portioner til frembringelse af et antal amplikoner, hvor (i) opformeringen udføres med en population af dNTPer, der omfatter dNTP-analoger, således at et antal nukleotider i DNAet erstattes med dNTP-analogerne, og (ii) opformeringen udføres i nærvær af et additiv, der er udvalgt blandt glycogen, DMSO, ET SSB, betain og en hvilken som helst kombination deraf, (c) fjernelse af dNTP-analogerne fra amplikonerne til frembringelse af gap-holdigt DNA, (d) behandling af den gap-holdige DNA-syre til translation af gaps, indtil gaps på modsatte strenge løber sammen til derved frembringelse af stump-endede DNA-fragmenter, hvor dNTP-analogerne inkluderer både deoxyuracil og 5-methylcytosin.
9. Fremgangsmåde ifølge krav 8, hvor fremgangsmåden yderligere omfatter opnåelse af et antal sekvenslæsninger fra fragmenter fra hver separate portion.
10. Fremgangsmåde ifølge krav 9, hvor fragmenterne fra hver separate portion før denne opnåelse opformeres en anden gang.
11. Fremgangsmåde ifølge krav 8-10, hvor fragmenterne har længder på fra ca. 10.000 til ca. 200.000 bp.
12. Fremgangsmåde ifølge krav 8-11, hvor fragmenterne har længder på ca. 100.000 bp.
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| PCT/US2010/038741 WO2010148039A2 (en) | 2009-06-15 | 2010-06-15 | Methods and compositions for long fragment read sequencing |
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| HK1169679A1 (en) | 2013-02-01 |
| AU2010260088B2 (en) | 2016-02-11 |
| EP2977455B1 (en) | 2020-04-15 |
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