DK2479271T3 - Glycerol-3-phosphat-acyltransferase - Google Patents

Glycerol-3-phosphat-acyltransferase Download PDF

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DK2479271T3
DK2479271T3 DK10817312.1T DK10817312T DK2479271T3 DK 2479271 T3 DK2479271 T3 DK 2479271T3 DK 10817312 T DK10817312 T DK 10817312T DK 2479271 T3 DK2479271 T3 DK 2479271T3
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amino acid
seq
acid sequence
polynucleotide
glycerol
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DK10817312.1T
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Misa Ochiai
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Suntory Holdings Ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D7/00—Edible oil or fat compositions containing an aqueous phase, e.g. margarines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A23L31/10—Yeasts or derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
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    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
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    • C12P7/00—Preparation of oxygen-containing organic compounds
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    • C12P7/6409—Fatty acids
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01015—Glycerol-3-phosphate O-acyltransferase (2.3.1.15)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
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Claims (14)

1. Polynucleotid ifølge et vilkårligt udvalgt fra gruppen bestående af (a) til (d) nedenfor: (a) et polynucleotid, som omfatter nucleotidsekvensen ifølge SEQ ID NO:1 eller 4, og som koder for et protein som har en glycerol-3-phosphat-acyltransferase-aktivitet; (b) et polynucleotid, som koder for et protein, som består af aminosyresekvensen ifølge SEQ ID NO:2, og som haren glycerol-3-phosphat-acyltransferase-aktivitet; (c) et polynucleotid, som koder for et protein, som består af en aminosyresekvens, hvor 1 til 70 aminosyrer er deleterede, substituerede, indsat og/eller tilføjet i aminosyresekvensen ifølge SEQ ID NO:2, og som har en glycerol-3-phosphat-acyltrans-ferase-aktivitet; og (d) et polynucleotid, som koder for et protein, som har en aminosyresekvens med i det mindste 90% homologi med aminosyresekvensen ifølge SEQ ID NO:2, og som har en glycerol-3-phosphat-acyltransferase-aktivitet.
2. Polynucleotid ifølge krav 1, hvor polynucleotidet koder for et protein, som består af aminosyresekvens, hvor 1 til 10 aminosyrer er deleterede, substituerede, indsat og/eller tilføjet i aminosyresekvensen ifølge SEQ ID NO:2, og som har en glycerol-3-phosphat-acyltransferase-aktivitet.
3. Polynucleotid ifølge krav 1, som omfatter nucleotidsekvensen ifølge SEQ ID NO:1 eller 4.
4. Polynucleotid ifølge krav 1, som koder for et protein, som består af aminosyresekvensen ifølge SEQ ID NO:2.
5. Polynucleotid ifølge ethvert af kravene 1 til 4, som er et DNA.
6. Protein valgt fra gruppen bestående af (a) til (c) nedenfor: (a) et protein, som omfatter aminosyresekvensen ifølge SEQ ID NO:2, og som har en glycerol-3-phosphat-acyltransferase-aktivitet; (b) et protein, som indeholder en aminosyresekvens, hvor 1 til 70 aminosyrer er deleterede, substituerede, indsat og/eller tilføjet i aminosyresekvensen ifølge SEQ ID NO:2, og som har en glycerol-3-phosphat-acyltransferase-aktivitet; og (c) et protein, som har en aminosyresekvens, som har i det mindste 90% homologi med aminosyresekvensen ifølge SEQ ID NO:2, og som har en glycerol-3-phosphat-acyltransferase-aktivitet.
7. Vektor omfattende polynucleotidet ifølge ethvert af kravene 1 til 5.
8. Ikke-human transformant, i hvilken polynucleotidet ifølge ethvert af kravene 1 til 5 er indført, som indeholder det nævnte polynucleotid og eksprimerer dette.
9. Ikke-human transformant, i hvilken vektoren ifølge krav 7 er indført, og som indeholder den nævnte vektor og som eksprimerer det nævnte polynucleotid omfattet i vektoren.
10. Transformant ifølge krav 8 eller 9, hvor transformanten er en lipidproducerende svamp.
11. Transformant ifølge krav 10, hvor den lipidproducerende svamp er Mortierella alpina.
12. Transformant ifølge krav 8 eller 9, som er en gær.
13. Fremgangsmåde til fremstilling af en lipid- eller fedtsyresammensætning, som omfatter udvinding af lipid- eller fedtsyresammensætningen fra kulturen af transformanten i overensstemmelse med ethvert af kravene 10 til 12.
14. Fremgangsmåde til fremstilling af en fødevare, et lægemiddel, et kosmetikum eller en sæbe, som omfatter trinnet med at udføre fremgangsmåden ifølge krav 13 til fremstilling af en lipid- eller fedtsyresammensætning, og yderligere omfattende trinnet med at anvende den således producerede lipid- eller fedtsyresammensætning til fremstilling af en fødevare, et lægemiddel, et kosmetikum eller en sæbe.
DK10817312.1T 2009-09-18 2010-09-21 Glycerol-3-phosphat-acyltransferase DK2479271T3 (da)

Applications Claiming Priority (2)

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JP2009217646 2009-09-18
PCT/JP2010/066280 WO2011034199A1 (ja) 2009-09-18 2010-09-21 グリセロール-3-リン酸アシル基転移酵素

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DK2479271T3 true DK2479271T3 (da) 2017-01-16

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EP (1) EP2479271B1 (da)
JP (1) JP5193371B2 (da)
KR (1) KR101399242B1 (da)
CN (1) CN102753685B (da)
AU (1) AU2010296296B2 (da)
BR (1) BR112012005913A2 (da)
CA (1) CA2774408C (da)
DK (1) DK2479271T3 (da)
RU (1) RU2499835C1 (da)
WO (1) WO2011034199A1 (da)

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WO2011096481A1 (ja) 2010-02-03 2011-08-11 サントリーホールディングス株式会社 グリセロール－３－リン酸アシル基転移酵素ホモログとその利用
CN112852647B (zh) * 2021-02-23 2022-06-07 广东省科学院微生物研究所(广东省微生物分析检测中心) 一种适用于拟茎点霉fs508的过表达载体及其构建方法和应用

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ATE356213T1 (de) * 1999-04-01 2007-03-15 Basf Plant Science Gmbh Enzyme des triacylglycerol-biosynthesewegs und dafür kodierende rekombinante dna moleküle
AU5666500A (en) * 1999-06-21 2001-01-09 National Research Council Of Canada Overexpression in yeast and plants of a gene encoding glycerol 3-phosphate acyltransferase
JP4036595B2 (ja) * 2000-03-03 2008-01-23 サントリー株式会社 ｎ−４系及び／又はｎ−７系高度不飽和脂肪酸を含有する脂質及びその製造方法
EP1613746B1 (de) * 2003-03-31 2013-03-06 University Of Bristol Neue pflanzliche acyltransferasen spezifisch für langkettige, mehrfach ungesättigte fettsäuren
JP4481702B2 (ja) 2004-03-31 2010-06-16 サントリーホールディングス株式会社 脂質生産菌の育種方法およびその利用
US7550651B2 (en) 2004-06-25 2009-06-23 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7192762B2 (en) 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7550286B2 (en) * 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
WO2008146745A1 (ja) 2007-05-25 2008-12-04 Suntory Holdings Limited 新規なリゾホスファチジン酸アシル基転移酵素遺伝子
JP5451387B2 (ja) * 2007-06-18 2014-03-26 サントリーホールディングス株式会社 グリセロール−３−リン酸アシル基転移酵素（ｇｐａｔ）ホモログとその利用
JP2009217646A (ja) 2008-03-11 2009-09-24 Ricoh Co Ltd 回路最適化方法
EP2676554B1 (en) 2009-03-26 2014-12-31 Suntory Holdings Limited Novel lysophospholipid acyltransferase

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WO2011034199A1 (ja) 2011-03-24
EP2479271B1 (en) 2016-11-02
KR101399242B1 (ko) 2014-05-27
AU2010296296A1 (en) 2012-04-12
RU2499835C1 (ru) 2013-11-27
CA2774408A1 (en) 2011-03-24
CN102753685A (zh) 2012-10-24
CN102753685B (zh) 2014-05-21
BR112012005913A2 (pt) 2015-09-08
EP2479271A4 (en) 2013-04-10
EP2479271A1 (en) 2012-07-25
US8980607B2 (en) 2015-03-17
CA2774408C (en) 2016-03-15
JP5193371B2 (ja) 2013-05-08
US20130252308A1 (en) 2013-09-26
RU2012115455A (ru) 2013-10-27
AU2010296296B2 (en) 2014-04-17
KR20120048023A (ko) 2012-05-14

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