DK2550366T3 - Fremgangsmåder til at identificere inhibitorer af typen iii sekretionssystem - Google Patents
Fremgangsmåder til at identificere inhibitorer af typen iii sekretionssystem Download PDFInfo
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- DK2550366T3 DK2550366T3 DK11710752.4T DK11710752T DK2550366T3 DK 2550366 T3 DK2550366 T3 DK 2550366T3 DK 11710752 T DK11710752 T DK 11710752T DK 2550366 T3 DK2550366 T3 DK 2550366T3
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Claims (8)
1. Fremgangsmåde til at bestemme hvorvidt en testforbindelse har evnen til at inhibere funktionen af typen 3 sekretionssystem T3SS, hvor en testforbindelse bringes i kontakt med bakterieceller der har et T3SS og testes, a. i et første trin, for dets evne til at inhibere sekretion af et effektorprotein i bakteriecellerne ved bestemmelse af mængden af et udskilt effektorprotein på grund af dets binding til dets beslægtede chaperon-molekyle, hvor en reduceret mængde effektorprotein bundet til chaperonen som sammenlignet med mængden i en kontrolprøve fra cellerne der ikke er blevet behandlet med testforbindelsen er indikativ for en inhibitorisk virkning af forbindelsen på sekretion af effektorproteinet, og b. i et andet trin, for dets evne til at inhibere samlingen af strukturkomponenterne for at danne T3SS-nålekomplekset.
2. Fremgangsmåden ifølge krav 1, hvor effektoren er valgt fra SptP- og SipA-Flag og chaperonen er valgt fra henholdsvis SicP og InvB.
3. Fremgangsmåden ifølge kravene 1 eller 2, hvor mængden af udskilt effektor- eller translokatorprotein bestemmes ved et ELISA.
4. Fremgangsmåden ifølge krav 3, hvor ELISA konverteres til et Amplified Luminescent Proximity Homogeneous Assay og løber i højgennemløbsformat.
5. Fremgangsmåde til overvågning af samlingen strukturkomponenter for at danne T3SS-nålekomplekset, hvor samlingen af nålekomplekset bestemmes ved at detektere en senere-fastgjort strukturalkomponent [x] når den er forbundet med henholdsvis en forud-eksisterende strukturalkomponent [x-1], eller med et forud-dannet kompleks der indeholder komponent [x-1].
6. Fremgangsmåden ifølge krav 1, hvor i det andet trin, samlingen af nålekomplekset bestemmes ved at detektere en senere-fastgjort strukturalkomponent [x] når den er forbundet med henholdsvis en forud-eksisterende strukturkomponent [x-1], eller med et forud-dannet kompleks der indeholder komponent [x-1].
7. Fremgangsmåden ifølge krav 5 eller 6, hvor fastgørelsen af komponent [x-1] til komponent [x] bestemmes i et ELISA, hvor komponent [x-1] eller et kompleks indeholdende det konjugeres på en fast bærer og komponent [x] er detekteret når fastgjort til [x-1].
8. Fremgangsmåde ifølge et hvilket som helst af kravene 5 til 7, hvor Prgi fra Salmonella typhimurium eller en homolog deraf fra en anden gram-negativ bakterie er komponent [x] og hvor PrgH fra Salmonella typhimurium eller en homolog deraf fra en anden gramnegativ bakterie er komponent [x-1].
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10157397 | 2010-03-23 | ||
| PCT/EP2011/054379 WO2011117259A1 (en) | 2010-03-23 | 2011-03-22 | Methods for identifying inhibitors of the type iii secretion system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2550366T3 true DK2550366T3 (da) | 2014-12-15 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11710752.4T DK2550366T3 (da) | 2010-03-23 | 2011-03-22 | Fremgangsmåder til at identificere inhibitorer af typen iii sekretionssystem |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20130130283A1 (da) |
| EP (1) | EP2550366B1 (da) |
| JP (1) | JP5714692B2 (da) |
| CA (1) | CA2793586A1 (da) |
| DK (1) | DK2550366T3 (da) |
| ES (1) | ES2526316T3 (da) |
| WO (1) | WO2011117259A1 (da) |
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| US5581476A (en) | 1993-01-28 | 1996-12-03 | Amgen Inc. | Computer-based methods and articles of manufacture for preparing G-CSF analogs |
| ES2389625T3 (es) * | 1996-04-23 | 2012-10-29 | The University Of British Columbia | Vacuna que comprende la proteína EspA asociada a Escherichia coli patógena |
| AU3243199A (en) | 1998-03-05 | 1999-09-20 | University Of British Columbia, The | Methods for assaying type iii secretion inhibitors |
| US6248546B1 (en) * | 1998-03-09 | 2001-06-19 | Diagnostic Systems Laboratories, Inc. | Assay of IGFBP complex |
| GB0112687D0 (en) * | 2001-05-24 | 2001-07-18 | Microbiological Res Authority | Pharmaceutical use of secreted bacterial effector proteins |
| GB0304993D0 (en) * | 2003-03-05 | 2003-04-09 | Univ Nottingham Trent | Novel screening method |
| WO2005113791A2 (en) | 2004-05-07 | 2005-12-01 | Johnson & Johnson Product Research And Development | Methods of identifying modulators of bacterial type iii protein secretion system |
| EP1795609A1 (en) * | 2005-12-06 | 2007-06-13 | Sanofi-Aventis Deutschland GmbH | Method for the diagnosis and treatment of cardiovascular diseases |
| CA2563214A1 (en) * | 2006-10-12 | 2008-04-12 | Institut Pasteur | Shigella ipad protein and its use as a potential vaccine against shigella infection |
| WO2009061491A2 (en) | 2007-11-07 | 2009-05-14 | University Of Massachusetts | Type iii secretion inhibitors and uses thereof |
| WO2009145829A1 (en) | 2008-04-01 | 2009-12-03 | University Of Massachusetts | Type iii secretion inhibitors, analogs and uses thereof |
| WO2009137133A2 (en) | 2008-02-14 | 2009-11-12 | University Of Washington | 5-substituted-2-imino-thiazolidinone compounds and their use as inhibitors of bacterial infection |
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- 2011-03-22 ES ES11710752.4T patent/ES2526316T3/es active Active
- 2011-03-22 CA CA2793586A patent/CA2793586A1/en not_active Abandoned
- 2011-03-22 US US13/636,567 patent/US20130130283A1/en not_active Abandoned
- 2011-03-22 WO PCT/EP2011/054379 patent/WO2011117259A1/en not_active Ceased
- 2011-03-22 DK DK11710752.4T patent/DK2550366T3/da active
- 2011-03-22 EP EP11710752.4A patent/EP2550366B1/en not_active Not-in-force
Also Published As
| Publication number | Publication date |
|---|---|
| JP5714692B2 (ja) | 2015-05-07 |
| WO2011117259A9 (en) | 2011-11-24 |
| WO2011117259A1 (en) | 2011-09-29 |
| US20130130283A1 (en) | 2013-05-23 |
| EP2550366A1 (en) | 2013-01-30 |
| JP2013522638A (ja) | 2013-06-13 |
| ES2526316T3 (es) | 2015-01-09 |
| CA2793586A1 (en) | 2011-09-29 |
| EP2550366B1 (en) | 2014-11-12 |
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