DK2552943T3 - Effektiv fremstilling af rekombinante autonome parvovira ved brug af en fra adenovirus afledt hjælper-vektor - Google Patents
Effektiv fremstilling af rekombinante autonome parvovira ved brug af en fra adenovirus afledt hjælper-vektor Download PDFInfo
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- DK2552943T3 DK2552943T3 DK11716475.6T DK11716475T DK2552943T3 DK 2552943 T3 DK2552943 T3 DK 2552943T3 DK 11716475 T DK11716475 T DK 11716475T DK 2552943 T3 DK2552943 T3 DK 2552943T3
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Claims (6)
1. Fremgangsmåde til fremstilling af en rekombinant autonom parvovirus kendetegnet ved, at 5 (i) HEK 293T-celler undergår transfektion med (a) en rekombinant parvovirus-vektor afledt fra rotte-parvovirus H-1 (H-1) eller muse-minute-virus (MVM), idet der mangler Kapsid-virus- 10 proteinets VP1- og VP2-gener, men hvor der er bevaret det ikke-strukturelle proteins NSl/2-kodende sekvenser, (b) en vektor, som indeholder generne, der koder parvovirus-VPl- og -VP2-Kapsid-proteiner, og (c) en fra adenovirus afledt hjælper-vektor omfattende de adenovirale Ad5 15 DNA-sekvenser fra E2a-genet, E4(orf6)-genet og et VAI RNA-gen; og (ii) den rekombinante parvovirus, som mangler VP1- og VP2-generne, men bevarer de NSl/2-kodende sekvenser, efter celledyrkningen befinder sig isoleret fra HEK 293T-celleme eller mediet. 20
2. Fremgangsmåde ifølge krav 1, hvor i den rekombinante parvovirus-vektor VP1- og VP2-geneme er erstattet af et transgen.
3. Fremgangsmåde ifølge krav 2, hvor expressionen af transgenet står under kontrol af 25 P38-promoteren.
4. Fremgangsmåde ifølge krav 2 eller 3, hvor transgenet er et gen, som koder et markør-protein.
5. Fremgangsmåde ifølge krav 2 eller 3, hvor transgenet er et gen, som koder en tera peutisk eller immunogenisk polypeptid.
6. Fremgangsmåde ifølge krav 5, hvor det terapeutiske protein er en cytotoksisk polypeptid, cytokin og/eller chemokin. 35
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10003296.0A EP2368903B1 (en) | 2010-03-26 | 2010-03-26 | Adenovirus derived helper virus for enhancing recombinant parvovirus production |
| PCT/EP2011/001493 WO2011116974A2 (en) | 2010-03-26 | 2011-03-24 | Adenovirus derived helper virus for enhancing recombinant parvovirus production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2552943T3 true DK2552943T3 (da) | 2018-08-13 |
Family
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10003296.0T DK2368903T3 (da) | 2010-03-26 | 2010-03-26 | Adenovirusafledt hjælpevirus til fremme af produktion af rekombinant parvovirus |
| DK11716475.6T DK2552943T3 (da) | 2010-03-26 | 2011-03-24 | Effektiv fremstilling af rekombinante autonome parvovira ved brug af en fra adenovirus afledt hjælper-vektor |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
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| DK10003296.0T DK2368903T3 (da) | 2010-03-26 | 2010-03-26 | Adenovirusafledt hjælpevirus til fremme af produktion af rekombinant parvovirus |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9803219B2 (da) |
| EP (2) | EP2368903B1 (da) |
| JP (1) | JP5829265B2 (da) |
| AU (1) | AU2011231998B2 (da) |
| CA (1) | CA2794383C (da) |
| DK (2) | DK2368903T3 (da) |
| ES (2) | ES2461147T3 (da) |
| WO (1) | WO2011116974A2 (da) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2368903T3 (da) * | 2010-03-26 | 2014-05-05 | Deutsches Krebsforsch | Adenovirusafledt hjælpevirus til fremme af produktion af rekombinant parvovirus |
| DK2620503T3 (da) * | 2012-01-27 | 2014-12-15 | Deutsches Krebsforsch | Modificeret parvovirus anvendelig til genslukning |
| EP3327124A1 (en) * | 2016-11-28 | 2018-05-30 | Deutsches Krebsforschungszentrum | H-1 pv expressing rnai effectors targeting cdk9 |
| EP3754015A1 (en) * | 2019-06-17 | 2020-12-23 | Deutsches Krebsforschungszentrum, Stiftung des öffentlichen Rechts | Optimized parvovirus h-1 production |
| WO2022007800A1 (zh) * | 2020-07-06 | 2022-01-13 | 嘉兴安宇生物科技有限公司 | 一种非洲猪瘟的重组腺病毒疫苗及其构建方法 |
| WO2022250491A1 (en) * | 2021-05-27 | 2022-12-01 | Neuracle Genetics Inc. | Novel dual helper plasmid |
| US12305188B2 (en) | 2021-05-27 | 2025-05-20 | Neuracle Genetics Inc. | Dual helper plasmid |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6004797A (en) * | 1995-11-09 | 1999-12-21 | Avigen, Inc. | Adenovirus helper-free recombinant AAV Virion production |
| ATE329046T1 (de) * | 1998-10-14 | 2006-06-15 | Deutsches Krebsforsch | Parvovirus-vektoren und deren verwendung |
| CA2385823A1 (en) * | 1999-10-01 | 2001-04-12 | Haifeng Chen | Production of recombinant aav using adenovirus comprising aav rep/cap genes |
| US7115391B1 (en) | 1999-10-01 | 2006-10-03 | Genovo, Inc. | Production of recombinant AAV using adenovirus comprising AAV rep/cap genes |
| CA2369985A1 (en) * | 2002-01-18 | 2003-07-18 | Duke University | Generation of recombinant adeno-associated viral vectors by a complete adenovirus-mediated approach |
| DK2368903T3 (da) * | 2010-03-26 | 2014-05-05 | Deutsches Krebsforsch | Adenovirusafledt hjælpevirus til fremme af produktion af rekombinant parvovirus |
-
2010
- 2010-03-26 DK DK10003296.0T patent/DK2368903T3/da active
- 2010-03-26 EP EP10003296.0A patent/EP2368903B1/en active Active
- 2010-03-26 ES ES10003296.0T patent/ES2461147T3/es active Active
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2011
- 2011-03-24 AU AU2011231998A patent/AU2011231998B2/en not_active Ceased
- 2011-03-24 CA CA2794383A patent/CA2794383C/en active Active
- 2011-03-24 EP EP11716475.6A patent/EP2552943B1/en active Active
- 2011-03-24 JP JP2013501673A patent/JP5829265B2/ja not_active Expired - Fee Related
- 2011-03-24 WO PCT/EP2011/001493 patent/WO2011116974A2/en not_active Ceased
- 2011-03-24 ES ES11716475.6T patent/ES2683002T3/es active Active
- 2011-03-24 DK DK11716475.6T patent/DK2552943T3/da active
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2012
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Also Published As
| Publication number | Publication date |
|---|---|
| ES2683002T3 (es) | 2018-09-24 |
| EP2368903B1 (en) | 2014-03-05 |
| US9803219B2 (en) | 2017-10-31 |
| AU2011231998B2 (en) | 2015-07-02 |
| CA2794383A1 (en) | 2011-09-29 |
| JP5829265B2 (ja) | 2015-12-09 |
| US20130089523A1 (en) | 2013-04-11 |
| US20170073704A9 (en) | 2017-03-16 |
| EP2552943B1 (en) | 2018-05-09 |
| CA2794383C (en) | 2015-12-01 |
| DK2368903T3 (da) | 2014-05-05 |
| ES2461147T3 (es) | 2014-05-16 |
| JP2013539957A (ja) | 2013-10-31 |
| WO2011116974A3 (en) | 2012-01-05 |
| AU2011231998A1 (en) | 2012-10-25 |
| EP2552943A2 (en) | 2013-02-06 |
| EP2368903A1 (en) | 2011-09-28 |
| WO2011116974A2 (en) | 2011-09-29 |
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