DK2562247T3 - PCR-indretning med to varmeblokke - Google Patents
PCR-indretning med to varmeblokke Download PDFInfo
- Publication number
- DK2562247T3 DK2562247T3 DK11772265.2T DK11772265T DK2562247T3 DK 2562247 T3 DK2562247 T3 DK 2562247T3 DK 11772265 T DK11772265 T DK 11772265T DK 2562247 T3 DK2562247 T3 DK 2562247T3
- Authority
- DK
- Denmark
- Prior art keywords
- pcr
- chip
- plate
- approx
- pcr device
- Prior art date
Links
- 238000010438 heat treatment Methods 0.000 title claims description 106
- 238000003752 polymerase chain reaction Methods 0.000 claims description 193
- 230000003321 amplification Effects 0.000 claims description 49
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 49
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
- B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
- C12M1/38—Temperature-responsive control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Clinical Laboratory Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (14)
1. Polymerasekædereaktion (PCR)-indretning (1) omfattende: en første varmeblok (100), der er anbragt på et substrat (400); en anden varmeblok (200), der er anbragt adskilt fra den første varmeblok på substratet (400); en chipholder (300), der kan bevæges venstre-højre og/eller op-ned ved hjælp af et drivmiddel (500) over den første og anden varmeblok og er installeret med en chip (900) til PCR, hvor chippen til PCR kan aftages fra chipholderen (300), hvor chippen (900) til PCR er fremstillet til at komme i kontakt med en overflade af den første varmeblok (100) eller den anden varmeblok (200) og til at indeholde en prøveopløsning, der indeholder nukleinsyre, der som amplifice-res, kendetegnet ved, at en lyskilde (700) endvidere er indført mellem den første og anden varmeblok, og en lysdetekteringsenhed (800) til detektering af lyset, der udsendes fra lyskilden (700), endvidere er anbragt over chipholderen (300); eller lysdetekteringsenheden (800) til detektering af lyset, der udsendes fra lyskilden (700), endvidere er indført mellem den første og anden varmeblok, og lyskilden (700) endvidere er anbragt over chipholderen (300).
2. PCR-indretning (1) ifølge krav 1, hvor en varmetråd er anbragt i hver af den første og anden varmeblok.
3. PCR-indretning (1) ifølge krav 2, hvor varmetråden er anbragt symmetrisk i op-ned- og/eller venstre-højre-retninger i forhold til et centrum af hver overflade af den første og anden varmeblok for jævnt at opretholde en samlet temperatur inden i den første eller anden varmeblok.
4. PCR-indretning (1) ifølge krav 1, hvor den første og anden varmeblok (100, 200) er fremstillet til at opretholde en denatureringstemperatur af PCRen.
5. PCR-indretning (1) ifølge krav 4, hvor denatureringstemperaturen er fra ca. 90 til ca. 100 °C.
6. PCR-indretning (1) ifølge krav 1, hvor den første og anden varmeblok (100, 200) er fremstillet til at opretholde en udglødnings- og forlængelses-(eller forstærknings-)temperatur af PCRen.
7. PCR-indretning (1) ifølge krav 6, hvor udglødnings- og forlængelses- (eller forstærknings-)temperaturen er fra ca. 55 til ca. 75 °C.
8. PCR-indretning (1) ifølge krav 1, hvor den første og anden varmeblok (100, 200) er anbragt adskilt i en forudbestemt afstand, således at der ikke sker nogen varmeudveksling derimellem.
9. PCR-indretning (1) ifølge krav 1, hvor drivmidlet (500) omfatter; en skinne (510), der strækker sig i venstre-højre-retningen; og et forbindelseselement (520), der er anbragt for at muliggøre en glidende bevægelse i venstre-højre-retningen via skinnen (510) og muliggør en glidende bevægelse i op-ned-retningen, hvor chipholderen (300) er anbragt i en ende af forbindelseselementet (520).
10. PCR-indretning (1) ifølge krav 1, hvor chippen (900) til PCR er et lysoverførende plastmateriale.
11. PCR-indretning (1) ifølge krav 1, hvor PCR-chippen (900) omfatter: en første plade (910); en anden plade (920), der er anbragt på den første plade (910) og omfatter gennemgangsåbningskanaler (921); og en tredje plade (930), der er anbragt på den anden plade (920) og omfatter en gennemgangsåbningsindgangsenhed (931), der er udformet i forhold til en del af den udformede gennemgangsåbningskanal, og en gennemgangsåbningsudgangsenhed (932), der er udformet i forhold til den anden del af gennemgangsåbningskanalen.
12. PCR-indretning (1) ifølge krav 11, hvor den første plade (910) og tredje plade (930) omfatter et materiale, der er valgt fra gruppen bestående af po-lydimethylsiloxan (PDMS), cyklisk olefincopolymer (COC), polymethyl-methacrylat (PMMA), polycarbonat (PC), polypropylencarbonat (PPC), poly- ethersulfon (PES), polyethylenterephthalat (PET) og en kombination deraf, og den anden plade omfatter et termoplastisk resin eller termohærdende re-sinmateriale, der er valgt fra gruppen bestående af polymethylmethacrylat (PMMA), polycarbonat (PC), cyklisk olefincopolymer (COC), polyamid (PA), polyethylen (PE), polypropylen (PP), polyphenylenether (PPE), polystyren (PS), polyoxymethylen (POM), polyetheretherketon (PEEK), polytetrafluo-rethylen (PTFE), polyvinylchlorid (PVC), polyvinylidenfluorid (PVDF), poly-butylenterephthalat (PBT), fluorineret ethylenpropylen (FEP), perfluoral-koxyalkan (PFA) og en kombination deraf.
13. PCR-indretning (1) ifølge krav 11, en diameter af gennemgangsåbningsindgangsenheden (931) af den tredje plade (930) er valgt fra ca. 1,0 mm til ca. 3,0 mm, en diameter af gennemgangsåbningsudgangsenhederne (932) er valgt fra ca. 1,0 mm til ca. 1,5 mm, en tykkelse af den tredje plade (930) er valgt fra ca. 0,1 mm til ca. 2 mm, en tykkelse af den anden pladen (920) er valgt fra ca. 100 pm til ca. 200 pm, en bredde af gennemgangsåbningskanalerne (921) er valgt fra ca. 0,5 mm til ca. 3 mm, og en længde af gennemgangsåbningskanalen (921) er valgt fra ca. 20 mm til ca. 40 mm.
14. PCR-indretning (1) ifølge krav 10, hvor en overføringsenhed (530) til at overføre lyset, der udsendes fra lyskilden (700), er anbragt i drivmidlet (500).
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20100037960 | 2010-04-23 | ||
| KR20110037352A KR101368463B1 (ko) | 2010-04-23 | 2011-04-21 | 2개의 열 블록을 포함하는 ｐｃｒ 장치 |
| PCT/KR2011/002911 WO2011132977A2 (ko) | 2010-04-23 | 2011-04-22 | 2개의 열 블록을 포함하는 pcr 장치 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2562247T3 true DK2562247T3 (da) | 2016-04-18 |
Family
ID=45032143
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11772265.2T DK2562247T3 (da) | 2010-04-23 | 2011-04-22 | PCR-indretning med to varmeblokke |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US9061285B2 (da) |
| EP (1) | EP2562247B1 (da) |
| JP (1) | JP5661918B2 (da) |
| KR (1) | KR101368463B1 (da) |
| CN (1) | CN102985527B (da) |
| DK (1) | DK2562247T3 (da) |
| ES (1) | ES2563805T3 (da) |
| HU (1) | HUE027355T2 (da) |
| RS (1) | RS54641B1 (da) |
| WO (1) | WO2011132977A2 (da) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101456646B1 (ko) * | 2011-12-09 | 2014-11-03 | 나노바이오시스 주식회사 | 식중독균 검출용 키트 및 이를 이용한 식중독균 검출 방법 |
| KR101969076B1 (ko) * | 2012-02-01 | 2019-08-13 | 주식회사 미코바이오메드 | 광 노이즈 제거 수단이 구현된 실시간 ｐｃｒ 칩 및 이를 포함하는 ｐｃｒ 장치 |
| KR101404455B1 (ko) * | 2012-07-04 | 2014-06-10 | 나노바이오시스 주식회사 | 전기화학적 신호를 검출하기 위한 실시간 ｐｃｒ 장치, 및 이를 이용한 실시간 ｐｃｒ 방법 |
| KR102003784B1 (ko) * | 2012-10-19 | 2019-07-25 | 주식회사 미코바이오메드 | 마이크로 ｐｃｒ 칩 및 이를 포함하는 실시간 ｐｃｒ 장치 |
| KR102028381B1 (ko) * | 2012-12-27 | 2019-10-04 | 주식회사 미코바이오메드 | 식중독 검출용 프라이머 세트를 이용한 pcr 장치, 및 이를 이용한 식중독 검출 방법 |
| KR101427038B1 (ko) * | 2013-03-04 | 2014-08-06 | 이현영 | 핵산 증폭장치 |
| KR101483493B1 (ko) * | 2013-03-22 | 2015-01-19 | 나노바이오시스 주식회사 | 식중독 검출용 프라이머 세트, 이를 이용한 pcr 장치, 및 이를 이용한 식중독 검출 방법 |
| KR102078085B1 (ko) * | 2013-12-31 | 2020-02-17 | 주식회사 미코바이오메드 | 농식품의 식중독균 검출용 랩온어칩 기반의 초고속 실시간 pcr 장치, 및 이를 이용한 식중독 검출방법 |
| KR101618113B1 (ko) * | 2014-02-10 | 2016-05-09 | 나노바이오시스 주식회사 | 일 방향 슬라이딩 구동 수단을 구비하는 pcr 장치 및 이를 이용하는 pcr 방법 |
| CN105316226A (zh) * | 2014-05-31 | 2016-02-10 | 捷普科技（上海）有限公司 | 升降机构及具备该升降机构的生物芯片检测装置 |
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| US9297040B2 (en) | 2016-03-29 |
| JP5661918B2 (ja) | 2015-01-28 |
| KR20110118572A (ko) | 2011-10-31 |
| CN102985527B (zh) | 2015-11-25 |
| ES2563805T3 (es) | 2016-03-16 |
| HUE027355T2 (en) | 2016-09-28 |
| EP2562247A4 (en) | 2014-03-05 |
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