DK2631336T3 - DNA-bibliotek og fremgangsmåde til fremstilling heraf samt fremgangsmåde og anordning til detektering af SNP - Google Patents
DNA-bibliotek og fremgangsmåde til fremstilling heraf samt fremgangsmåde og anordning til detektering af SNP Download PDFInfo
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- DK2631336T3 DK2631336T3 DK11843141.0T DK11843141T DK2631336T3 DK 2631336 T3 DK2631336 T3 DK 2631336T3 DK 11843141 T DK11843141 T DK 11843141T DK 2631336 T3 DK2631336 T3 DK 2631336T3
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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Claims (8)
1. Fremgangsmåde til fremstilling af et DNA-bibliotek, hvilken fremgangsmåde omfatter følgende trin: fordøjelse af en genomisk DNA-prøve ved anvendelse af en restriktionsendonuklease udvalgt fra: (1) Mbo II (2) Mbo II og Hind III, (3) Mbo II og Bcc I eller (4) Tsp 451 for at opnå et fordøjet produkt; fraseparering af det fordøjede produkt for at opnå DNA-fragmenter med en længde på 100 bp til 1000 bp\ ende-reparation af DNA-fragmenteme for at opnå ende-reparerede DNA-fragmenter; tilføjelse af en base A til enden af de ende-reparerede DNA-fragmenter for at opnå DNA-fragmenter med en terminal base A; og ligering af DNA-fragmenteme med den terminale base A med sekvenseringsadaptere for at opnå DNA-biblioteket.
2. Fremgangsmåde ifølge krav 1, hvor det fordøjede produkt frasepareres ved hjælp af agarose-gelelektroforese og genvinding ved gelskæring.
3. Fremgangsmåde ifølge krav 1, hvor DNA-fragmenteme har en længde på 200 bp til 700 bp.
4. Fremgangsmåde til opnåelse af genomiske sekvensinformationer fra en prøve af genomisk DNA, hvilken fremgangsmåde omfatter opbygning af et DNA-bibliotek i henhold til en fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3 og sekvensering af DNA-biblioteket for at opnå DNA-sekvensinformationeme.
5. Fremgangsmåde ifølge krav 4, hvor DNA-biblioteket sekvenseres på en sekvenseringsplatform udvalgt fra Illumina, Roche 454 og SOLID.
6. Fremgangsmåde ifølge krav 4 eller krav 5, hvilken fremgangsmåde endvidere omfatter et trin med udsættelse af DNA-sekvensinformationer for SNP-dataanalyse for at opnå SNP-informationer.
7. Fremgangsmåde ifølge krav 6, hvilken fremgangsmåde endvidere omfatter genotypebestemmelse af prøven baseret på SNP-informationeme.
8. Kit til detektering af SNP omfattende restriktionsendonukleaser udvalgt fra (1) Mbo II og Hind III eller (2) Mbo II og Bcc I.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010555192.4A CN102061526B (zh) | 2010-11-23 | 2010-11-23 | 一种DNA文库及其制备方法、以及一种检测SNPs的方法和装置 |
| PCT/CN2011/079971 WO2012068919A1 (zh) | 2010-11-23 | 2011-09-21 | DNA文库及其制备方法、以及检测SNPs的方法和装置 |
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| Publication Number | Publication Date |
|---|---|
| DK2631336T3 true DK2631336T3 (da) | 2015-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11843141.0T DK2631336T3 (da) | 2010-11-23 | 2011-09-21 | DNA-bibliotek og fremgangsmåde til fremstilling heraf samt fremgangsmåde og anordning til detektering af SNP |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9493821B2 (da) |
| EP (1) | EP2631336B1 (da) |
| CN (1) | CN102061526B (da) |
| DK (1) | DK2631336T3 (da) |
| WO (1) | WO2012068919A1 (da) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061526B (zh) * | 2010-11-23 | 2014-04-30 | 深圳华大基因科技服务有限公司 | 一种DNA文库及其制备方法、以及一种检测SNPs的方法和装置 |
| CN102242222B (zh) * | 2011-07-21 | 2013-05-15 | 中国水产科学研究院珠江水产研究所 | 基于est-ssr标记的草鱼分类方法 |
| CN102952854B (zh) * | 2011-08-25 | 2015-01-14 | 深圳华大基因科技有限公司 | 单细胞分类和筛选方法及其装置 |
| CN103160937B (zh) * | 2011-12-15 | 2015-02-18 | 深圳华大基因科技服务有限公司 | 对高等植物复杂基因组基因进行富集建库和snp分析的方法 |
| CN102534814B (zh) * | 2012-01-18 | 2013-08-28 | 首都医科大学 | 全细胞水平高效捕获染色质转录调控区的方法及其用途 |
| CN102691111B (zh) * | 2012-03-29 | 2014-11-26 | 首都医科大学 | 高通量全基因组水平捕获染色质核小体空缺区的方法 |
| WO2014019180A1 (zh) * | 2012-08-01 | 2014-02-06 | 深圳华大基因研究院 | 确定异常状态生物标记物的方法及系统 |
| CN103627710B (zh) * | 2012-08-22 | 2016-08-03 | 中国人民解放军总医院 | Spg11基因突变体及其应用 |
| CN106029899B (zh) * | 2013-09-30 | 2021-08-03 | 深圳华大基因股份有限公司 | 确定染色体预定区域中snp信息的方法、系统和计算机可读介质 |
| CN103572378B (zh) * | 2013-10-28 | 2015-12-02 | 博奥生物集团有限公司 | 基于Ion ProtonTM 测序平台的小片段DNA文库的构建方法及其应用 |
| CN104005090B (zh) * | 2014-05-28 | 2016-08-17 | 北京诺禾致源生物信息科技有限公司 | 低质量样本dna高通量测序文库的构建方法 |
| CN105506748B (zh) * | 2016-01-18 | 2018-11-27 | 北京百迈客生物科技有限公司 | 一种dna高通量测序建库方法 |
| CN105603535B (zh) * | 2016-01-27 | 2018-11-27 | 北京诺禾致源科技股份有限公司 | 构建dna文库的试剂盒和方法 |
| CN106148513A (zh) * | 2016-06-22 | 2016-11-23 | 杭州杰毅麦特医疗器械有限公司 | 一种游离dna文库构建方法及试剂盒 |
| CN108300764B (zh) * | 2016-08-30 | 2021-11-09 | 武汉康昕瑞基因健康科技有限公司 | 一种建库方法及snp分型方法 |
| CN108300773A (zh) * | 2016-08-30 | 2018-07-20 | 广州康昕瑞基因健康科技有限公司 | 一种建库方法及snp分型方法 |
| CN108179174A (zh) * | 2018-01-15 | 2018-06-19 | 武汉爱基百客生物科技有限公司 | 一种高通量简化基因组测序文库的构建方法 |
| CN109610011A (zh) * | 2018-12-28 | 2019-04-12 | 厦门胜芨科技有限公司 | 一种NanoDNA超长伴随建库试剂盒及其使用方法 |
| CN111199773B (zh) * | 2020-01-20 | 2023-03-28 | 中国农业科学院北京畜牧兽医研究所 | 一种精细定位性状关联基因组纯合片段的评估方法 |
| CN113337590B (zh) * | 2021-06-03 | 2024-07-09 | 深圳华大基因股份有限公司 | 一种二代测序方法和文库构建方法 |
| CN114356222B (zh) * | 2021-12-13 | 2022-08-19 | 深圳先进技术研究院 | 数据存储方法、装置、终端设备及计算机可读存储介质 |
Family Cites Families (12)
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|---|---|---|---|---|
| US5710000A (en) * | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
| CN1188526C (zh) | 2001-09-30 | 2005-02-09 | 湖北大学 | 一种基因克隆的高效方法 |
| CN103937899B (zh) * | 2005-12-22 | 2017-09-08 | 凯津公司 | 用于基于aflp的高通量多态性检测的方法 |
| US7579155B2 (en) | 2007-09-12 | 2009-08-25 | Transgenomic, Inc. | Method for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule |
| KR20090033307A (ko) | 2007-09-29 | 2009-04-02 | 재단법인서울대학교산학협력재단 | Ｂ형 간염 유래 간질환 진단용 키트 |
| CN101230490B (zh) | 2008-02-26 | 2013-01-30 | 北京林业大学 | 一种染色体步移文库的构建方法 |
| CN101343667A (zh) | 2008-07-11 | 2009-01-14 | 中国水产科学研究院黄海水产研究所 | 一种水产动物snp标记筛选方法 |
| WO2010085774A1 (en) * | 2009-01-26 | 2010-07-29 | Board Of Regents, The University Of Texas System | Digital restriction enzyme analysis of methylation |
| US8685648B2 (en) | 2009-02-03 | 2014-04-01 | Biohelix Corporation | Endonuclease-enhanced helicase-dependent amplification |
| EP2248914A1 (en) * | 2009-05-05 | 2010-11-10 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | The use of class IIB restriction endonucleases in 2nd generation sequencing applications |
| CN101845489A (zh) * | 2009-12-03 | 2010-09-29 | 中国海洋大学 | 一种大规模筛查扇贝snp的方法 |
| CN102061526B (zh) | 2010-11-23 | 2014-04-30 | 深圳华大基因科技服务有限公司 | 一种DNA文库及其制备方法、以及一种检测SNPs的方法和装置 |
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2010
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2011
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- 2011-09-21 US US13/989,031 patent/US9493821B2/en active Active
- 2011-09-21 EP EP11843141.0A patent/EP2631336B1/en active Active
- 2011-09-21 DK DK11843141.0T patent/DK2631336T3/da active
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| US20130288907A1 (en) | 2013-10-31 |
| EP2631336A4 (en) | 2013-10-16 |
| CN102061526B (zh) | 2014-04-30 |
| CN102061526A (zh) | 2011-05-18 |
| EP2631336B1 (en) | 2014-11-19 |
| US9493821B2 (en) | 2016-11-15 |
| EP2631336A1 (en) | 2013-08-28 |
| WO2012068919A1 (zh) | 2012-05-31 |
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