DK2682476T3 - Fremgangsmåde til kvantificering af kolesterol i højdensitetslipoprotein 2 og anvendelse af et reagenskit til fremgangsmåden - Google Patents

Fremgangsmåde til kvantificering af kolesterol i højdensitetslipoprotein 2 og anvendelse af et reagenskit til fremgangsmåden Download PDF

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Publication number
DK2682476T3
DK2682476T3 DK12752957.6T DK12752957T DK2682476T3 DK 2682476 T3 DK2682476 T3 DK 2682476T3 DK 12752957 T DK12752957 T DK 12752957T DK 2682476 T3 DK2682476 T3 DK 2682476T3
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DK
Denmark
Prior art keywords
cholesterol
phospholipase
hdl2
hdl
reagent
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DK12752957.6T
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English (en)
Inventor
Maiko Higuchi
Yasuki Itoh
Original Assignee
Denka Seiken Kk
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Application filed by Denka Seiken Kk filed Critical Denka Seiken Kk
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Publication of DK2682476T3 publication Critical patent/DK2682476T3/da

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (5)

1. Fremgangsmåde til kvantificering af kolesterol i højdensitetslipoprotein 2, hvilken fremgangsmåde omfatter at lade phospholipase indvirke på højdensitetslipoprotein og at kvantificere kolesterol, hvor phospholipasen er phospholipase C og/eller phospholipase D.
2. Fremgangsmåde ifølge krav 1, hvor phospholipasen er reaktiv med mindst phosphatidylcholin.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor koncentrationen af phospholipasen er 0,1 til 200 U/ml.
4. Anvendelse af et kit omfattende phospholipase C og/eller phospholipase D til kvantificering af kolesterol i højdensitetslipoprotein 2 ved hjælp af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 3.
5. Fremgangsmåde til kvantificering af højdensitetslipoprotein 2 kolesterol i en testprøve, hvilken fremgangsmåde omfatter: et første trin med at overføre kolesteroler, som ikke er højdensitetslipoproteiner, i en testprøve til ydersiden af reaktionssystemet; og et andet trin med at kvantificere højdensitetslipoprotein 2 kolesterol blandt de højdensitetslipoproteiner, der er tilbage i reaktionssystemet; hvor det andet trin udføres ved hjælp af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 3.
DK12752957.6T 2011-02-28 2012-02-27 Fremgangsmåde til kvantificering af kolesterol i højdensitetslipoprotein 2 og anvendelse af et reagenskit til fremgangsmåden DK2682476T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2011042374 2011-02-28
PCT/JP2012/054797 WO2012118017A1 (ja) 2011-02-28 2012-02-27 高密度リポタンパク質２中のコレステロールの定量方法およびそのための試薬キット

Publications (1)

Publication Number Publication Date
DK2682476T3 true DK2682476T3 (da) 2017-04-24

Family

ID=46757951

Family Applications (1)

Application Number Title Priority Date Filing Date
DK12752957.6T DK2682476T3 (da) 2011-02-28 2012-02-27 Fremgangsmåde til kvantificering af kolesterol i højdensitetslipoprotein 2 og anvendelse af et reagenskit til fremgangsmåden

Country Status (7)

Country Link
US (2) US10031145B2 (da)
EP (1) EP2682476B1 (da)
JP (1) JP5956420B2 (da)
KR (1) KR101871209B1 (da)
CN (1) CN103608465B (da)
DK (1) DK2682476T3 (da)
WO (1) WO2012118017A1 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101598597B1 (ko) * 2014-03-14 2016-03-02 연세대학교 산학협력단 혈장 대사체를 이용한 고-ldl-콜레스테롤 질환 진단 장치 및 방법
JP6582388B2 (ja) * 2014-10-23 2019-10-02 東洋紡株式会社 高密度リポ蛋白コレステロールの測定方法
US20170010290A1 (en) * 2015-07-07 2017-01-12 Mohmed E. Ashmaig Methods of determining a high density lipoprotein phospholipid level in a sample
CN113528609B (zh) * 2021-06-22 2024-11-01 美康生物科技股份有限公司 谷胱甘肽还原酶检测试剂盒及其制备方法和应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2653755B2 (ja) * 1994-03-08 1997-09-17 協和メデックス株式会社 高密度リポ蛋白中のコレステロールの定量法
JP3288033B2 (ja) 1996-12-09 2002-06-04 デンカ生研株式会社 高密度リポ蛋白中のコレステロール定量用試薬
JP4708531B2 (ja) 2000-06-07 2011-06-22 シスメックス株式会社 Ｈｄｌ亜画分中のコレステロールの測定法
EP1477570B1 (en) * 2002-01-30 2008-01-16 DENKA SEIKEN Co., Ltd. Method of quantifying cholesterol in high density lipoprotein and reagent compositions
US7799537B2 (en) * 2004-11-29 2010-09-21 Otsuka Pharmaceutical Co., Ltd. Cholesterol measuring reagent containing a cholesterol esterase
TWI379904B (en) * 2005-02-14 2012-12-21 Kyowa Medex Co Ltd A method for quantifying cholesterol of remnant-like particles lipoprotein, reagent and kit
EP1930443A4 (en) * 2005-09-30 2008-10-29 Denka Seiken Kk METHOD FOR SELECTIVE AND SIMULTANEOUS QUANTIFICATION OF TWO SUBSTANCES IN A BIOLOGICAL SAMPLE
EP1959021A4 (en) * 2005-12-09 2009-07-22 Kyowa Medex Co Ltd METHOD FOR DETERMINING CHOLESTEROLEMIA IN LIPOPROTEIN OF THE REMNANT TYPE, REAGENT AND KIT
KR101394802B1 (ko) * 2007-02-28 2014-05-15 덴카 세이켄 가부시키가이샤 소립자 저비중 리포 단백의 정량 시약
KR101586505B1 (ko) * 2007-10-10 2016-01-18 덴카 세이켄 가부시키가이샤 소입자 고비중 ｌｄｌ 콜레스테롤의 정량 방법 및 키트
JP5500777B2 (ja) 2008-03-06 2014-05-21 デンカ生研株式会社 Ｈｄｌ３−ｃの測定方法及びｈｄｌ３−ｃ測定用試薬
US20130171674A1 (en) 2010-07-23 2013-07-04 Denka Seiken Co., Ltd. Method for quantifying the amount of cholesterol in high-density lipoprotein 3

Also Published As

Publication number Publication date
JPWO2012118017A1 (ja) 2014-07-07
WO2012118017A1 (ja) 2012-09-07
KR20140027108A (ko) 2014-03-06
CN103608465A (zh) 2014-02-26
CN103608465B (zh) 2017-05-10
US20180306823A1 (en) 2018-10-25
US10955426B2 (en) 2021-03-23
US10031145B2 (en) 2018-07-24
US20140045201A1 (en) 2014-02-13
EP2682476A1 (en) 2014-01-08
JP5956420B2 (ja) 2016-07-27
KR101871209B1 (ko) 2018-06-27
EP2682476B1 (en) 2017-02-01
EP2682476A4 (en) 2015-06-17

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