DK2778231T3 - Fremgangsmåde til observering af stamceller, fremgangsmåde til fjernelse af celleområde i tilstand, der tenderer mod differentiering, og anordning til observering af stamceller - Google Patents
Fremgangsmåde til observering af stamceller, fremgangsmåde til fjernelse af celleområde i tilstand, der tenderer mod differentiering, og anordning til observering af stamceller Download PDFInfo
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- DK2778231T3 DK2778231T3 DK12847822.9T DK12847822T DK2778231T3 DK 2778231 T3 DK2778231 T3 DK 2778231T3 DK 12847822 T DK12847822 T DK 12847822T DK 2778231 T3 DK2778231 T3 DK 2778231T3
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- stem cells
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/088—Condensers for both incident illumination and transillumination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
- G01N2201/0446—Multicell plate, sequential
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Optics & Photonics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Microscoopes, Condenser (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Claims (8)
- FREMGANGSMÅDE TIL OBSERVERING AF STAMCELLER, FREMGANGSMÅDE TIL FJERNELSE AF CELLEOMRÅDE I TILSTAND, DER TENDERER MOD DIFFERENTIERING, OG ANORDNING TIL OBSERVERING AF STAMCELLER1. Fremgangsmåde til observering af stamceller, der omfatter: placering (SI) af stamceller (C) i et transparent element (11); montering (S2) af det transparente element (11) på et lysføringselement (21) via et optisk medium; udsendelse (S5) af belysningslys (LI) ind i lysføringselementet (21) og udsendelse af belysningslyset til stamcellerne i det transparente element, der indeholder en opløsning (12), via det optiske medium; og detektering (S6) af spredt lys (L2), hvor det spredte lys er belysningslyset udsendt til stamcellerne, der er spredt af stamcellerne og har passeret gennem lysføringselementet (21).
- 2. Fremgangsmåde til observering af stamceller ifølge krav 1, hvor, ved udsendelse (S5) af belysningslyset (LI) til stamcellerne, belysningslyset, der bestråler stamcellerne, udsendes diagonalt i forhold til en retning for montering af det transparente element på lysføringselementet.
- 3. Fremgangsmåde til observering af stamceller ifølge krav 2, hvor en udsendelsesvinkel for belysningslyset (LI), der er udsendt diagonalt til stamcellerne, er mellem 53° og 90° i forhold til monteringsretningen.
- 4. Fremgangsmåde til observering af stamceller ifølge et hvilket som helst af kravene 1 til 3, hvor, ved detektering (S6) af det spredte lys (L2), et billede af det spredte lys, der er spredt af stamcellerne og har passeret gennem lysføringselementet, opfanges af en billeddannende enhed.
- 5. Fremgangsmåde til observering af stamceller ifølge krav 4, der endvidere omfatter: at holde (S2) det transparente element med en holdemekanisme; visning (S5) af det spredte lysbillede, der er opnået af billeddannelsesenheden af en visningsenhed; modtagelse (S6) af en valgindlæsning i forhold til et vist celleområde i det spredte lysbillede vist i visningen, og visning af en markør svarende til det således valgte område på billedet; fjernelse (S7) af lysføringselementet fra under det transparente element efter opfangning af det spredte lysbillede af stamcellerne; opnåelse (S8) af et transmitteret lysbillede af stamcellerne ved hjælp af billeddannelsesenheden og visning af det transmitterede lysbillede ved hjælp af visningsenheden; og visning (S9) af markøren over det transmitterede lysbillede vist i visningen af det transmitterede lysbillede.
- 6. Fremgangsmåde til observering af stamceller ifølge krav 5, hvor, i visningen (S9) af markøren over det transmitterede lysbillede, et vist område af det transparente element svarende til markøren på det transmitterede lysbillede markeres.
- 7. Fremgangsmåde til fjernelse, der omfatter observering af stamceller i det transparente element ved hjælp af fremgangsmåden til observering af stamceller ifølge et hvilket som helst af kravene 1 til 6 og fjernelse, fra blandt stamcellerne, et celleområde, der er identificeret som værende i en tilstand, der tenderer mod differentiering ved observationen.
- 8. Anordning (1) til observering af stamceller anbragt i et transparent element, hvilken anordning omfatter: et lysføringselement (21) med en overflade, hvorpå det transparente element kan monteres; en første lyskilde (22), der er udformet til at udsende belysningslys (LI) ind i lysføringselementet (21) og udsende belysningslyset til stamcellerne i det transparente element, der indeholder en opløsning (12), via et optisk medium placeret mellem det transparente element og lysføringselementet, en bevægelsesenhed (23), der er udformet til at bevæge lysføringselementet; en holdeenhed (10), der er udformet til at holde det transparente element, når lysføringselementet bevæges af bevægelsesenheden; en anden lyskilde (20B), der er udformet til at udsende belysningslys (L3) til stamcellerne, der er anbragt i det transparente element holdt af holdeenheden; og en billeddannelsesenhed (30), der er udformet til at afbilde hvert af spredt lys (L2), hvor det spredte lys er belysningslyset fra den første lyskilde, der er spredt af stamcellerne og har passeret gennem lysføringselementet, og det transmitterede lys (L4), hvor det transmitterede lys er belysningslyset fra den anden lyskilde, der har passeret gennem stamcellerne.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011244867 | 2011-11-08 | ||
| PCT/JP2012/077365 WO2013069452A1 (ja) | 2011-11-08 | 2012-10-23 | 幹細胞の観察方法、分化傾向状態の細胞領域の除去方法、及び、幹細胞の観察装置 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2778231T3 true DK2778231T3 (da) | 2019-04-15 |
Family
ID=48289835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12847822.9T DK2778231T3 (da) | 2011-11-08 | 2012-10-23 | Fremgangsmåde til observering af stamceller, fremgangsmåde til fjernelse af celleområde i tilstand, der tenderer mod differentiering, og anordning til observering af stamceller |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US9134296B2 (da) |
| EP (1) | EP2778231B1 (da) |
| JP (2) | JP5955855B2 (da) |
| DK (1) | DK2778231T3 (da) |
| WO (1) | WO2013069452A1 (da) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105283754A (zh) * | 2013-06-03 | 2016-01-27 | 文塔纳医疗系统公司 | 用于组织检测的荧光成像系统 |
| DE102013107297B4 (de) * | 2013-07-10 | 2025-04-24 | Carl Zeiss Microscopy Gmbh | Anordnung zur Lichtblattmikroskopie |
| JP5775550B2 (ja) * | 2013-08-29 | 2015-09-09 | 株式会社Ｓｃｒｅｅｎホールディングス | 撮像装置および撮像方法 |
| JP2015146747A (ja) * | 2014-02-05 | 2015-08-20 | 浜松ホトニクス株式会社 | 細胞判定方法 |
| JP6301194B2 (ja) | 2014-05-26 | 2018-03-28 | 浜松ホトニクス株式会社 | 光学プレート、光照射装置、光測定装置、光照射方法、及び、光測定方法 |
| JP6284832B2 (ja) * | 2014-06-20 | 2018-02-28 | 富士フイルム株式会社 | 細胞評価装置および方法並びにプログラム |
| JP6391340B2 (ja) * | 2014-07-22 | 2018-09-19 | オリンパス株式会社 | 顕微鏡 |
| JP6543954B2 (ja) * | 2015-02-24 | 2019-07-17 | シンフォニアテクノロジー株式会社 | 除去領域設定方法、除去領域設定装置およびプログラム |
| US11371071B2 (en) | 2017-08-08 | 2022-06-28 | International Business Machines Corporation | Cell culturing structure including growth medium and non-growth medium |
| JP6541085B1 (ja) | 2018-06-29 | 2019-07-10 | 株式会社片岡製作所 | 細胞処理装置 |
| JP7318632B2 (ja) * | 2020-12-25 | 2023-08-01 | 横河電機株式会社 | 培養容器及び観察システム |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3049254B2 (ja) | 1990-02-08 | 2000-06-05 | シスメックス株式会社 | ２種類の光源を備えた光学式粒子分析装置 |
| JP3460673B2 (ja) * | 2000-02-04 | 2003-10-27 | 浜松ホトニクス株式会社 | 特定の遺伝子を発現した生細胞の選択的分離方法 |
| JP4084135B2 (ja) | 2002-09-04 | 2008-04-30 | 浜松ホトニクス株式会社 | 植物性細胞の育成状態測定装置および育成状態測定方法 |
| WO2005059091A1 (ja) | 2003-12-18 | 2005-06-30 | Hitachi Medical Corporation | 細胞培養装置 |
| US7564546B2 (en) * | 2004-06-30 | 2009-07-21 | Chemimage Corporation | Dynamic imaging of biological cells and other subjects |
| JP5032792B2 (ja) * | 2006-05-22 | 2012-09-26 | 浜松ホトニクス株式会社 | 細胞選別装置 |
| KR100768038B1 (ko) * | 2006-10-24 | 2007-10-17 | 한국전기연구원 | 균일한 내부 전반사 조명에 의한 바이오칩 측정 장치 및방법 |
| US8642306B2 (en) | 2007-06-14 | 2014-02-04 | Mitsui Engineering & Shipbuilding Co., Ltd. | Flow cytometer having cell-sorting function and method of separating living cells |
| WO2009036418A1 (en) * | 2007-09-13 | 2009-03-19 | Duke University | Apparatuses, systems, and methods for low-coherence interferometry (lci) |
| WO2009157385A1 (ja) * | 2008-06-27 | 2009-12-30 | 古河電気工業株式会社 | 細胞の識別およびソーティング方法およびその装置 |
| US8795981B2 (en) * | 2008-08-08 | 2014-08-05 | Molecular Devices, Llc | Cell detection |
| DK200801722A (en) * | 2008-12-05 | 2010-06-06 | Unisensor As | Optical sectioning of a sample and detection of particles in a sample |
| KR101747983B1 (ko) * | 2009-08-22 | 2017-06-15 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | 배아, 난모세포, 그리고 줄기 세포의 이미징과 평가 |
| EP2487249B1 (en) * | 2009-10-09 | 2018-06-13 | Kawasaki Jukogyo Kabushiki Kaisha | Method and apparatus for discrimination of undifferentiated pluripotent stem cells, and automatic culture method and apparatus |
| CN102712890B (zh) * | 2010-01-20 | 2016-03-30 | 株式会社尼康 | 细胞观察装置和细胞培养方法 |
-
2012
- 2012-10-23 JP JP2013542915A patent/JP5955855B2/ja active Active
- 2012-10-23 WO PCT/JP2012/077365 patent/WO2013069452A1/ja not_active Ceased
- 2012-10-23 US US14/356,487 patent/US9134296B2/en active Active
- 2012-10-23 DK DK12847822.9T patent/DK2778231T3/da active
- 2012-10-23 EP EP12847822.9A patent/EP2778231B1/en active Active
-
2015
- 2015-07-23 US US14/806,999 patent/US9435734B2/en active Active
-
2016
- 2016-06-15 JP JP2016119014A patent/JP6134422B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP5955855B2 (ja) | 2016-07-20 |
| EP2778231A1 (en) | 2014-09-17 |
| WO2013069452A1 (ja) | 2013-05-16 |
| US20140287452A1 (en) | 2014-09-25 |
| US9134296B2 (en) | 2015-09-15 |
| US20150323454A1 (en) | 2015-11-12 |
| JP6134422B2 (ja) | 2017-05-24 |
| JP2016168052A (ja) | 2016-09-23 |
| EP2778231B1 (en) | 2019-01-23 |
| JPWO2013069452A1 (ja) | 2015-04-02 |
| EP2778231A4 (en) | 2015-08-12 |
| US9435734B2 (en) | 2016-09-06 |
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