DK2806022T3 - Rekombinant mikroorganisme med forbedret putrescinproduktivitet og fremgangsmåde til fremstilling af putrescin under anvendelse af samme - Google Patents
Rekombinant mikroorganisme med forbedret putrescinproduktivitet og fremgangsmåde til fremstilling af putrescin under anvendelse af samme Download PDFInfo
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- DK2806022T3 DK2806022T3 DK13738280.0T DK13738280T DK2806022T3 DK 2806022 T3 DK2806022 T3 DK 2806022T3 DK 13738280 T DK13738280 T DK 13738280T DK 2806022 T3 DK2806022 T3 DK 2806022T3
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/001—Amines; Imines
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/15—Corynebacterium
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
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- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (8)
1. Rekombinant mikroorganisme af Corynebacterium glutamicum, som har evne til at producere putrescin, hvor mikroorganismen er blevet modificeret således, at et protein med en aminosyresekvens repræsenteret ved SEQ ID NO: 17 eller SEQ ID NO: 19, eller ekspressionen deraf, modificeres i mikroorganismen for at svække eller fjerne aktiviteten af proteinet og øge produktionen af putrescin sammenlignet med aktiviteten af proteinet og produktionen af putrescin i den samme, men umodificerede mikroorganismestamme, hvor proteinets aktivitet svækkes ved 1) en delvis eller hel deletion af et polynukieotid, som koder for proteinet, 2) en reduktion af polynukleotidekspressionen, 3) en modifikation af polynukleotidsekvensen på kromosomet for at svække proteinets aktivitet eller 4) en kombination deraf.
2. Mikroorganisme ifølge krav 1, hvor aktiviteten af ornithin-decarboxylase (SpeC) yderligere er indført dertil.
3. Mikroorganisme ifølge krav 2, hvor ornithin-decarboxylasen har aminosyresekven-sen repræsenteret ved SEQ ID NO: 22.
4. Mikroorganisme ifølge krav 1, hvor aktiviteterne i den rekombinante mikroorganisme af én eller flere valgt fra gruppen bestående af ornithin-carbamoyltransferase (ArgF) og glutamateksportør (NCgll 221) er yderligere svækket sammenlignet med aktiviteten af det samme protein i den samme, men umodificerede stamme.
5. Mikroorganisme ifølge krav 4, hvor ArgF har aminosyresekvensen repræsenteret ved SEQ ID NO: 20, og NCgl1221 har aminosyresekvensen repræsenteret ved SEQ ID NO: 21.
6. Mikroorganisme ifølge krav 1, hvor aktiviteterne i den rekombinante mikroorganisme af én eller flere valgt fra gruppen bestående af acetyl-gamma-glutamylphosphat-reduktase (ArgC), acetylglutamatsynthase eller ornithinacetyltransferase (ArgJ), ace-tylglutamatkinase (ArgB) og acetylornithin-aminotransferase (ArgD) er yderligere forbedret sammenlignet med aktiviteten af det samme protein i den samme, men umodificerede stamme.
7. Mikroorganisme ifølge krav 6, hvor ArgC, ArgJ, ArgB og ArgD har aminosyresekven-serne repræsenteret ved henholdsvis SEQ ID NO: 23, 24, 25 og 26.
8. Fremgangsmåde til fremstilling af putrescin omfattende dyrkning, for at fremstille en celledyrkningsvæske, af en modificeret mikroorganisme af Corynebacterium glutamicum, som har evne til at producere putrescin, hvor mikroorganismen er blevet modificeret således, at et protein med en aminosyresekvens repræsenteret ved SEQ ID NO: 17 eller SEQ ID NO: 19, eller ekspression deraf, modificeres i mikroorganismen for at svække eller fjerne aktiviteten af proteinet og øge produktionen af putrescin sammenlignet med aktiviteten af proteinet og produktionen af putrescin i den samme, men umodificerede mikroorganismestamme, hvor proteinets aktivitet svækkes ved 1) en delvis eller hel deletion af et poiynukleotid, som koder for proteinet, 2) en reduktion af polynukleotidekspressionen, 3) en modifikation af polynukleotidsekvensen på kromosomet for at svække proteinets aktivitet eller 4) en kombination deraf; og isolering af putrescin fra den opnåede celledyrkningsvæske.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20120007004 | 2012-01-20 | ||
| PCT/KR2013/000487 WO2013109121A1 (ko) | 2012-01-20 | 2013-01-21 | 퓨트레신 생산능이 향상된 재조합 미생물 및 이를 이용하여 퓨트레신을 생산하는 방법 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2806022T3 true DK2806022T3 (da) | 2018-08-13 |
Family
ID=48799483
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK13738280.0T DK2806022T3 (da) | 2012-01-20 | 2013-01-21 | Rekombinant mikroorganisme med forbedret putrescinproduktivitet og fremgangsmåde til fremstilling af putrescin under anvendelse af samme |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US9290771B2 (da) |
| EP (1) | EP2806022B1 (da) |
| JP (2) | JP6251187B2 (da) |
| KR (1) | KR101493586B1 (da) |
| AU (1) | AU2013210136B2 (da) |
| BR (1) | BR112014017910B1 (da) |
| DK (1) | DK2806022T3 (da) |
| ES (1) | ES2682696T3 (da) |
| IN (1) | IN2014MN01621A (da) |
| MY (1) | MY173018A (da) |
| PL (1) | PL2806022T3 (da) |
| RU (1) | RU2603089C2 (da) |
| TR (1) | TR201810509T4 (da) |
| WO (1) | WO2013109121A1 (da) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101607741B1 (ko) | 2013-03-20 | 2016-03-31 | 씨제이제일제당 (주) | 퓨트레신 생산 재조합 미생물 및 이를 이용한 퓨트레신 생산방법 |
| AU2015250431B2 (en) * | 2014-04-25 | 2017-09-28 | Cj Cheiljedang Corporation | Microorganisms for Producing Putrescine and Process for Producing Putrescine Using Them |
| KR101773135B1 (ko) * | 2015-05-08 | 2017-08-30 | 씨제이제일제당 주식회사 | 1,4-디아미노부탄의 정제방법 |
| US12139733B2 (en) | 2018-12-27 | 2024-11-12 | Cj Cheiljedang Corporation | Ornithine decarboxylase variant and method for producing putrescine by using same |
| WO2020138543A1 (ko) * | 2018-12-27 | 2020-07-02 | 씨제이제일제당 (주) | 오르니틴 탈탄산 효소의 변이주 및 그의 응용 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR920007401B1 (ko) | 1990-06-21 | 1992-08-31 | 제일제당 주식회사 | 다발성 절단부위를 지닌 대장균과 코리네형 세균에서 발현 가능한 신규 셔틀벡터 |
| CN101010433B (zh) | 2004-07-15 | 2012-03-07 | 帝斯曼知识产权资产管理有限公司 | 1,4-丁二胺的生物化学合成 |
| KR100620092B1 (ko) | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
| CN101523679B (zh) | 2006-09-30 | 2012-06-13 | 欧司朗股份有限公司 | 具有辅助保护电路的电源和电子镇流器 |
| KR100860932B1 (ko) | 2007-02-08 | 2008-09-29 | 씨제이제일제당 (주) | 신규한 프로모터 및 이의 용도 |
| MY175283A (en) * | 2008-04-10 | 2020-06-18 | Korea Advanced Inst Sci & Tech | Mutant microorganisms having high ability to produce putrescine and method for producing putrescine using the same |
| KR101188432B1 (ko) * | 2008-04-10 | 2012-10-08 | 한국과학기술원 | 퓨트레신 고생성능을 가지는 변이 미생물 및 이를 이용한 퓨트레신의 제조방법 |
| KR101348461B1 (ko) | 2010-12-08 | 2014-01-08 | 씨제이제일제당 (주) | 퓨트레신을 생산하는 미생물 및 이를 이용하여 퓨트레신을 생산하는 방법 |
-
2013
- 2013-01-21 PL PL13738280T patent/PL2806022T3/pl unknown
- 2013-01-21 RU RU2014133769/10A patent/RU2603089C2/ru active
- 2013-01-21 KR KR20130006707A patent/KR101493586B1/ko active Active
- 2013-01-21 JP JP2014553263A patent/JP6251187B2/ja active Active
- 2013-01-21 MY MYPI2014002114A patent/MY173018A/en unknown
- 2013-01-21 BR BR112014017910-7A patent/BR112014017910B1/pt active IP Right Grant
- 2013-01-21 AU AU2013210136A patent/AU2013210136B2/en active Active
- 2013-01-21 WO PCT/KR2013/000487 patent/WO2013109121A1/ko not_active Ceased
- 2013-01-21 DK DK13738280.0T patent/DK2806022T3/da active
- 2013-01-21 TR TR2018/10509T patent/TR201810509T4/tr unknown
- 2013-01-21 US US14/373,265 patent/US9290771B2/en active Active
- 2013-01-21 EP EP13738280.0A patent/EP2806022B1/en active Active
- 2013-01-21 IN IN1621MUN2014 patent/IN2014MN01621A/en unknown
- 2013-01-21 ES ES13738280.0T patent/ES2682696T3/es active Active
-
2016
- 2016-07-29 JP JP2016150247A patent/JP2017006133A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015506686A (ja) | 2015-03-05 |
| AU2013210136A1 (en) | 2014-09-11 |
| WO2013109121A1 (ko) | 2013-07-25 |
| US9290771B2 (en) | 2016-03-22 |
| AU2013210136B2 (en) | 2016-07-07 |
| PL2806022T3 (pl) | 2018-12-31 |
| JP2017006133A (ja) | 2017-01-12 |
| BR112014017910A2 (pt) | 2017-11-28 |
| KR101493586B1 (ko) | 2015-02-16 |
| RU2014133769A (ru) | 2016-03-20 |
| BR112014017910B1 (pt) | 2022-04-26 |
| EP2806022A1 (en) | 2014-11-26 |
| ES2682696T3 (es) | 2018-09-21 |
| IN2014MN01621A (da) | 2015-07-03 |
| CN104136600A (zh) | 2014-11-05 |
| EP2806022B1 (en) | 2018-05-23 |
| KR20130086010A (ko) | 2013-07-30 |
| EP2806022A4 (en) | 2015-10-07 |
| US20140363859A1 (en) | 2014-12-11 |
| JP6251187B2 (ja) | 2017-12-20 |
| MY173018A (en) | 2019-12-19 |
| RU2603089C2 (ru) | 2016-11-20 |
| TR201810509T4 (tr) | 2018-08-27 |
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