DK2814924T3 - Fremgangsmåde til enzymatisk afslimning - Google Patents
Fremgangsmåde til enzymatisk afslimning Download PDFInfo
- Publication number
- DK2814924T3 DK2814924T3 DK13705756.8T DK13705756T DK2814924T3 DK 2814924 T3 DK2814924 T3 DK 2814924T3 DK 13705756 T DK13705756 T DK 13705756T DK 2814924 T3 DK2814924 T3 DK 2814924T3
- Authority
- DK
- Denmark
- Prior art keywords
- oil
- phospholipase
- enzyme
- bacillus
- crude
- Prior art date
Links
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Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/008—Refining fats or fatty oils by filtration, e.g. including ultra filtration, dialysis
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/04—Refining fats or fatty oils by chemical reaction with acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01004—Phospholipase A2 (3.1.1.4)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01032—Phospholipase A1 (3.1.1.32)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/04—Phosphoric diester hydrolases (3.1.4)
- C12Y301/04003—Phospholipase C (3.1.4.3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/04—Phosphoric diester hydrolases (3.1.4)
- C12Y301/04004—Phospholipase D (3.1.4.4)
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
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- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Fats And Perfumes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Edible Oils And Fats (AREA)
Claims (10)
1. Fremgangsmåde til reduktion af emulgerbarheden af planteolie i vandige faser, omfattende trinnene: a) at bringe den rå planteolie i kontakt med en sammensætning omfattende en første enzymkomponent omfattende mindst et phospholipid-spaltende enzym og en anden enzymkomponent omfattende mindst et ikke-phospholipid-spal-tende enzym, hvor den anden enzymkomponent er en a-amylase; b) at separere slimstofferne fra planteolien, hvor den rå planteolie før i-kontakt-bringelsen ifølge trin a) bringes i kontakt med vand og/eller syre, der sker dog ingen separering af den vandige fase før trin a), men den forkonditionerede råolie anvendes umiddelbart i trin a).
2. Fremgangsmåde ifølge et af kravene 1, hvor den første enzymkomponent vælges fra gruppen bestående af phospholipase A1, phospholipase A2, phospholipase C, phospholipase B, phospholipase D og acyltransferase.
3. Fremgangsmåde ifølge krav 2, hvor phospholipase Ai stammer fra Thermo-myces lanuginosus, Fusarium oxysporium, Aspergillus oryzae, Bacillus ce-reus, Bacillus subtilis, Clostridium perfringens, Listeria monocytogenes, Pseudomonas spezies, svinepankreas eller oksepankreas; og/eller phospholipase A2 uafhængigt stammer fra svinepankreas, oksepankreas, Streptomyces vi-olaceoruber, Naja mossambica, Thermomyces lanuginosus, Fusarium oxysporium, Aspergillus oryzae, Bacillus cereus, Bacillus subtilis, Clostridium perfringens, Listeria monocytogenes eller Pseudomonas spezies; og/eller phospholipase C uafhængigt stammer fra Bacillus cereus, Clostridium perfringens, Listeria monocytogenes, Thermomyces lanuginosus, Fusarium oxysporium, Aspergillus oryzae, Bacillus cereus eller Pseudomonas spezies; og/eller phospholipase B uafhængigt stammer fra Thermomyces lanuginosus, Fusarium oxysporium, Aspergillus oryzae, Bacillus cereus, Bacillus subtilis, Clostridium perfringens, Listeria monocytogenes, Pseudomonas spezies, svinepankreas eller oksepankreas.
4. Fremgangsmåde ifølge krav 2 eller 3, hvor phospholipase Ai stammer fra Thermomyces lanuginosus, Fusarium oxysporium, og/eller phospholipase A2 uafhængigt stammer fra svinepankreas, oksepankreas, Streptomyces viola-ceoruber eller Naja mossambica, og/eller phospholipase C uafhængigt stammer fra Bacillus cereus, Clostridium perfringens eller Listeria monocytogenes.
5. Fremgangsmåde ifølge et af kravene 1 -3, hvor alpha-amylasen spalter al-pha(1-4)glykosidiske, alpha(1-2)glykosidiske, alpha(1-6)glykosidiske, beta(1-3)glykosidiske, beta(1-4)glykosidiske og/eller beta(1-6)glykosidiske bindinger.
6. Fremgangsmåde ifølge et af de foregående krav, hvor alpha-amylasen er en alpha-amylase af Bacillus sp., Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus amyloliquefaciens, Bacillus stearothermophilus, Pseudomonas aeroginosus, Pseudomonas fluorescens, Aspergillus oryzae eller Aspergillus niger.
7. Fremgangsmåde ifølge et af de foregående krav, hvor forholdet mellem den første enzymkomponents enzymaktivitet og den anden enzymkomponents enzymaktivitet ligger i området fra 0,01 : 6 enheder/g olie til 6 : 0,01 enheder/g olie.
8. Fremgangsmåde ifølge et af de foregående krav, hvor den første og/eller anden enzymkomponent forekommer i understøttet form.
9. Fremgangsmåde ifølge et af de foregående krav, hvor også den første og/eller anden enzymkomponent separeres fra planteolien samtidig med slimstofferne.
10. Fremgangsmåde ifølge et af de foregående krav, hvor der i stedet for planteolie anvendes planteolieslim.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102012003031 | 2012-02-17 | ||
| PCT/EP2013/053199 WO2013121047A1 (de) | 2012-02-17 | 2013-02-18 | Verfahren für die enzymatische ölentschleimung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2814924T3 true DK2814924T3 (da) | 2018-07-16 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK13705756.8T DK2814924T3 (da) | 2012-02-17 | 2013-02-18 | Fremgangsmåde til enzymatisk afslimning |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US9677027B2 (da) |
| EP (1) | EP2814924B1 (da) |
| CN (1) | CN104245908B (da) |
| BR (1) | BR112014020280A8 (da) |
| CA (1) | CA2864776C (da) |
| DK (1) | DK2814924T3 (da) |
| ES (1) | ES2676455T3 (da) |
| HU (1) | HUE038858T2 (da) |
| IN (1) | IN2014KN01679A (da) |
| PL (1) | PL2814924T3 (da) |
| PT (1) | PT2814924T (da) |
| RU (1) | RU2582044C2 (da) |
| TR (1) | TR201810285T4 (da) |
| WO (1) | WO2013121047A1 (da) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104245908B (zh) | 2012-02-17 | 2017-03-01 | 科莱恩产品（德国）有限公司 | 用于酶法油脱胶的方法 |
| EP2792735A1 (de) * | 2013-04-16 | 2014-10-22 | Clariant Produkte (Deutschland) GmbH | Verfahren zur Verbesserung der wässrigen enzymatischen Entschleimung von Pflanzenölen |
| CN105349255A (zh) * | 2015-11-06 | 2016-02-24 | 华南理工大学 | 一种酶法脱胶方法 |
| CN106590920A (zh) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | 一种植物油脱胶剂的制备方法 |
| US20200010778A1 (en) * | 2017-03-20 | 2020-01-09 | Novozymes A/S | Method for Degumming and Refining of Vegetable Oil |
| EP3401383A1 (en) | 2017-05-08 | 2018-11-14 | Bunge Oils, Inc. | Process for enzymatic degumming |
| CN107699343A (zh) * | 2017-08-28 | 2018-02-16 | 浙江海洋大学 | 一种金枪鱼油的脱胶工艺 |
| CN107916256B (zh) * | 2017-12-07 | 2021-06-11 | 王艺璇 | 一种磷脂酶 |
| WO2019215078A1 (en) | 2018-05-07 | 2019-11-14 | Dsm Ip Assets B.V. | Process for enzymatic oil degumming |
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| GB1440462A (en) * | 1973-06-29 | 1976-06-23 | Stork Amsterdam | Process for the clarification and/or recovery of vegetable oils |
| LU83441A1 (fr) | 1981-06-19 | 1983-04-06 | Tirtiaux Sa | Procede de traitement des huiles et graisses et produits ainsi obtenus |
| EP0162404B1 (en) | 1984-05-15 | 1988-08-24 | Seitetsu Kagaku Co., Ltd. | Process for preparing 2-alkyl-5-haloacetylbenzenesulfonamide |
| US4803016A (en) | 1986-08-08 | 1989-02-07 | Central Soya Company, Inc. | Method of deoiling crude lecithin |
| DE4115938A1 (de) | 1991-05-16 | 1992-11-19 | Metallgesellschaft Ag | Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen |
| US5175210A (en) | 1991-06-14 | 1992-12-29 | Shell Oil Company | Polymer blends |
| US7226771B2 (en) | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| US20050059567A1 (en) | 2003-09-11 | 2005-03-17 | The Procter & Gamble Company | Methods of formulating enzyme cocktails, enzyme cocktails for the removal of egg-based and grass-based stains and/or soils, compositions and products comprising same |
| DE10353150A1 (de) | 2003-11-14 | 2005-06-16 | Cognis Deutschland Gmbh & Co. Kg | Verfahren zur gleichzeitigen Herstellung von Sterolen und polaren Lipiden |
| WO2005063950A1 (en) * | 2003-12-19 | 2005-07-14 | Bunge Oils, Inc. | Process for improving enzymatic degumming of vegetable oils and reducing fouling of downstream processing equipment |
| CN101052702B (zh) | 2004-07-16 | 2013-01-09 | 杜邦营养生物科学有限公司 | 脂肪分解酶及其在食品工业中的应用 |
| ATE342951T1 (de) * | 2004-08-06 | 2006-11-15 | Smet Engineering N V De | Verfahren zum rückgewinnen von öl |
| WO2007103005A1 (en) * | 2006-03-01 | 2007-09-13 | Cargill, Incorporated | Method for degumming triglyceride oils |
| WO2008024840A2 (en) | 2006-08-22 | 2008-02-28 | Dow Agrosciences Llc | Aqueous processing of oilseed press cake |
| EP2057274B1 (en) | 2006-09-21 | 2013-12-11 | DSM IP Assets B.V. | Phospholipases, nucleic acids encoding them and methods for making and using them |
| CN100513541C (zh) | 2006-10-18 | 2009-07-15 | 江南大学 | 一种水酶法提取亚麻籽油的方法 |
| DK2118248T3 (da) * | 2007-01-30 | 2014-11-03 | Bunge Oils Inc | Enzymatisk degummiering under anvendelse af en blanding af PLA- og PLC-phospholipaser |
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| UA111708C2 (uk) * | 2009-10-16 | 2016-06-10 | Бандж Ойлз, Інк. | Спосіб рафінування олії |
| DE102010055159A1 (de) | 2010-12-18 | 2012-06-21 | Lurgi Gmbh | Verfahren zur enzymatischen Reinigung von Ölen pflanzlicher oder tierischer Herkunft |
| CN104245908B (zh) | 2012-02-17 | 2017-03-01 | 科莱恩产品（德国）有限公司 | 用于酶法油脱胶的方法 |
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2013
- 2013-02-18 CN CN201380020328.3A patent/CN104245908B/zh not_active Expired - Fee Related
- 2013-02-18 RU RU2014137464/13A patent/RU2582044C2/ru not_active IP Right Cessation
- 2013-02-18 HU HUE13705756A patent/HUE038858T2/hu unknown
- 2013-02-18 PT PT137057568T patent/PT2814924T/pt unknown
- 2013-02-18 TR TR2018/10285T patent/TR201810285T4/tr unknown
- 2013-02-18 US US14/379,010 patent/US9677027B2/en not_active Expired - Fee Related
- 2013-02-18 BR BR112014020280A patent/BR112014020280A8/pt not_active Application Discontinuation
- 2013-02-18 EP EP13705756.8A patent/EP2814924B1/de not_active Not-in-force
- 2013-02-18 IN IN1679KON2014 patent/IN2014KN01679A/en unknown
- 2013-02-18 CA CA2864776A patent/CA2864776C/en not_active Expired - Fee Related
- 2013-02-18 PL PL13705756T patent/PL2814924T3/pl unknown
- 2013-02-18 DK DK13705756.8T patent/DK2814924T3/da active
- 2013-02-18 WO PCT/EP2013/053199 patent/WO2013121047A1/de not_active Ceased
- 2013-02-18 ES ES13705756.8T patent/ES2676455T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US9677027B2 (en) | 2017-06-13 |
| ES2676455T3 (es) | 2018-07-19 |
| EP2814924A1 (de) | 2014-12-24 |
| HUE038858T2 (hu) | 2018-12-28 |
| CN104245908A (zh) | 2014-12-24 |
| BR112014020280A2 (da) | 2017-06-20 |
| PT2814924T (pt) | 2018-07-10 |
| CA2864776A1 (en) | 2013-08-22 |
| TR201810285T4 (tr) | 2018-08-27 |
| IN2014KN01679A (da) | 2015-10-23 |
| CA2864776C (en) | 2016-10-18 |
| PL2814924T3 (pl) | 2018-11-30 |
| RU2014137464A (ru) | 2016-04-10 |
| US20150017708A1 (en) | 2015-01-15 |
| EP2814924B1 (de) | 2018-04-25 |
| BR112014020280A8 (pt) | 2017-07-11 |
| WO2013121047A1 (de) | 2013-08-22 |
| CN104245908B (zh) | 2017-03-01 |
| RU2582044C2 (ru) | 2016-04-20 |
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