DK2862933T3 - Bidirektionel promotor - Google Patents

Bidirektionel promotor Download PDF

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Publication number
DK2862933T3
DK2862933T3 DK14175932T DK14175932T DK2862933T3 DK 2862933 T3 DK2862933 T3 DK 2862933T3 DK 14175932 T DK14175932 T DK 14175932T DK 14175932 T DK14175932 T DK 14175932T DK 2862933 T3 DK2862933 T3 DK 2862933T3
Authority
DK
Denmark
Prior art keywords
bidirectional promoter
bidirectional
promoter
Prior art date
Application number
DK14175932T
Other languages
English (en)
Inventor
Thomas Kickenweiz
Lukas Sturmberger
Anton Glieder
Thomas Vogl
Original Assignee
Bisy E U
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bisy E U filed Critical Bisy E U
Application granted granted Critical
Publication of DK2862933T3 publication Critical patent/DK2862933T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK14175932T 2013-07-05 2014-07-07 Bidirektionel promotor DK2862933T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP13175416 2013-07-05

Publications (1)

Publication Number Publication Date
DK2862933T3 true DK2862933T3 (da) 2019-11-18

Family

ID=48747980

Family Applications (1)

Application Number Title Priority Date Filing Date
DK14175932T DK2862933T3 (da) 2013-07-05 2014-07-07 Bidirektionel promotor

Country Status (3)

Country Link
US (1) US9822357B2 (da)
EP (1) EP2862933B8 (da)
DK (1) DK2862933T3 (da)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3839055B1 (en) 2013-03-08 2023-08-23 Biogrammatics, Inc. Yeast promoters for protein expression
US10619164B2 (en) 2013-03-08 2020-04-14 Keck Graduate Institute Of Applied Life Sciences Yeast promoters from Pichia pastoris
DK3212786T3 (da) * 2014-10-28 2019-07-01 Acib Gmbh Kompakt og optimeret metaboliseringsvejsdesign i pichia pastoris
EP3217807A4 (en) 2014-11-11 2018-09-12 Clara Foods Co. Methods and compositions for egg white protein production
US11155824B2 (en) * 2015-09-29 2021-10-26 Bisy e.U. Episomal plasmid vectors
EP3184642B1 (de) 2015-12-22 2019-05-08 bisy e.U. Hefezelle
CA3008850A1 (en) 2017-06-29 2018-12-29 Modern Meadow, Inc. Yeast strains and methods for producing collagen
US11655476B2 (en) * 2017-09-29 2023-05-23 Victoria Link Llimited Modular DNA assembly system
WO2019203769A1 (en) * 2018-04-16 2019-10-24 Akdeniz Universitesi Determination of regulatory dna regions of alcohol dehydrogenase 3 (adh3) promoter and design of synthetic promoters for recombinant protein production
US11866714B2 (en) 2018-06-07 2024-01-09 Basf Se Promoter for yeast
SI3802820T1 (sl) * 2018-06-07 2023-05-31 Basf Se Postopek za prepoznavanje promotorjev za proizvodnjo proteinov v kvasovkah
WO2020068019A2 (en) * 2018-09-24 2020-04-02 Orta Dogu Teknik Universitesi Design of alcohol dehydrogenase 2 (adh2) promoter variants by promoter engineering
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
SI3997118T1 (sl) 2019-07-11 2025-06-30 Clara Foods Co. Sestavek napitka, ki obsega rekombinantni ovomukoidni protein
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
CN115678899B (zh) * 2021-07-23 2025-05-30 中国科学院青岛生物能源与过程研究所 一种来源于肠杆菌的双向启动子及其应用
WO2025247966A1 (en) 2024-05-29 2025-12-04 Mybios Gmbh Process for protein production in methylotrophic yeast host cell.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6630324B1 (en) * 1999-07-29 2003-10-07 Baylor College Of Medicine Aldehyde reductase bidirectional promoter and its use
EP1711612B1 (de) * 2003-07-22 2007-07-25 SunGene GmbH Expressionskassetten zur bidirektionalen transgenen expression von nukleinsäuren in pflanzen
US20130157308A1 (en) 2011-12-15 2013-06-20 University Of South Carolina Bidirectional promoter reporter vector for the analysis of dual regulatory elements

Also Published As

Publication number Publication date
EP2862933B1 (en) 2019-09-04
US9822357B2 (en) 2017-11-21
EP2862933A3 (en) 2015-07-22
US20150011407A1 (en) 2015-01-08
EP2862933B8 (en) 2019-12-11
EP2862933A2 (en) 2015-04-22

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