DK2970878T3 - Fremgangsmåder og reagenser til opretholdelse af levedygtigheden af cancerceller i operativt fjernet væv - Google Patents
Fremgangsmåder og reagenser til opretholdelse af levedygtigheden af cancerceller i operativt fjernet væv Download PDFInfo
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- DK2970878T3 DK2970878T3 DK14763797.9T DK14763797T DK2970878T3 DK 2970878 T3 DK2970878 T3 DK 2970878T3 DK 14763797 T DK14763797 T DK 14763797T DK 2970878 T3 DK2970878 T3 DK 2970878T3
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- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- A—HUMAN NECESSITIES
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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Claims (17)
1. Reagens til en operativt fjernet cancervævsprøve til muliggørelse af genekspressionsanalyse, hvilket reagens omfatter bestanddelene: mindst én chaotrop; mindst én kosmotrop; en chelator; en buffer; et apoptosesubstrat og en metabolisk modulator; hvor apoptosesubstratet er leptin; og hvor den mindst ene kosmotrop er α,α-trehalose.
2. Reagens ifølge krav 1, hvor slutkoncentrationen af den mindst ene chaotrop er fra 0,1 M til 2 M, slutkoncentrationen af den mindst ene kosmotrop er fra 0,1 M til 2 M, slutkoncentrationen af chelatoren er fra 0,1 M til 2 M, og slutkoncentrationen af apoptosesubstratet er fra 0,001 M til 0,5 M.
3. Reagens ifølge krav 1, hvor chelatoren findes ved en slutkoncentration på mindst 0,01 M.
4. Reagens ifølge krav 2, hvor den mindst ene chaotrop er natriumthiocyanat, den mindst ene kosmotrop er en kombination af glycerol og α,α-trehalose, chelatoren er EDTA, bufferen er natriumphosphat, og den metaboliske modulator er DMSO.
5. Reagens ifølge krav 1, hvor chaotropen er valgt fra gruppen, der består af natriumthiocyanat, H2PO4“, HCO3“, I“, Cl“, NO3-, NH4-, Cs, K+, (NH4)C+ guanidinium, alle salte af guanidinium, Br og Rb.
6. Reagens ifølge krav 1, hvor en yderligere kosmotrop er valgt fra gruppen, der består af glycerol, trimethylamin-N-oxid, ectoin, 3-dimethylsulfoniopropionat, glucose, dextran og D-lactose.
Ί. Reagens ifølge krav 1, hvor chelatoren er valgt fra gruppen, der består af EDTA, EGTA og BABTA.
8. Reagens ifølge krav 1, hvor bufferen er valgt fra gruppen, der består af monobasisk kaliumphosphat og tribasisk kaliumphosphat, BIS-TRIS, BIS-TRIS-propan, HEPES, HEPES-natriumsalt, MES, MES-natriumsalt, MOPS, MOPS-natriumsalt, natriumsalt og natriumphosphatbuffer.
9. Reagens ifølge krav 1, hvor den metaboliske modulator er valgt fra gruppen, der består af polære aprotiske opløsningsmidler, DMSO, acetone, N,N-dimethylformamid og acetonitril.
10. Fremgangsmåde til fremstilling af et reagens til stabilisering, konservering og forbedring af levedygtigheden af cancervæv, der er blevet operativt fjernet, hvilken fremgangsmåde omfatter: tilvejebringelse af mindst én chaotrop; tilvejebringelse af mindst én kosmotrop; tilvejebringelse af en chelator; tilvejebringelse af en buffer; tilvejebringelse af et apoptosesubstrat; tilvejebringelse af en metabolisk modulator; og blanding af chaotropen, kosmotropen, chelatoren, bufferen, apoptosesubstratet og den metaboliske modulator i en specifik rækkefølge til muliggørelse af genekspressionsanalyse af den operativt fjernede vævsprøve, der er anbragt i reagenset; hvor apoptosesubstratet er leptin; og hvor den mindst ene kosmotrop er α,α-trehalose.
11. Fremgangsmåde ifølge krav 10, hvor slutkoncentrationen af chaotropen er fra 0,1 M til 2 M, slutkoncentrationen af kosmotropen er fra 0,1 M til 2 M, slutkoncentrationen af chelatoren er fra 0,1 M til 2 M, og slutkoncentrationen af apoptosesubstratet er fra 0,001 M til 0,5 M.
12. Fremgangsmåde ifølge krav 10, hvor slutkoncentrationen af chelatoren er mindst 0,01 M.
13. Fremgangsmåde ifølge krav 10, hvor chaotropen er valgt fra gruppen, der består af natriumthiocyanat, fhPCh”, ΗΟΟ3“, I“, Cl“, NO3-, NH4-, Cs, K+, (NH4)C+ guanidinium, alle salte af guanidinium, Br og Rb.
14. Fremgangsmåde ifølge krav 10, hvor en yderligere kosmotrop er valgt fra gruppen, der består af glycerol, trimethylamin-N-oxid, ectoin, 3-dimethylsulfoniopropionat, glucose, dextran og D-lactose.
15. Fremgangsmåde ifølge krav 10, hvor chelatoren er valgt fra gruppen, der består af EDTA, EGTA og BABTA.
16. Fremgangsmåde ifølge krav 10, hvor bufferen er valgt fra gruppen, der består af monobasisk kaliumphosphat og tribasisk kaliumphosphat, BIS-TRIS, BIS-TRIS-propan, HEPES, HEPES-natriumsalt, MES, MES-natriumsalt, MOPS, MOPS-natriumsalt, natriumsalt og natriumphosphatbuffer.
17. Fremgangsmåde ifølge krav 10, hvor den metaboliske modulator er valgt fra gruppen, der består af polære aprotiske opløsningsmidler, DMSO, acetone, N,N-dimethylformamid og acetonitril.
Applications Claiming Priority (2)
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| US201361798627P | 2013-03-15 | 2013-03-15 | |
| PCT/US2014/029198 WO2014144682A1 (en) | 2013-03-15 | 2014-03-14 | Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue |
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| DK2970878T3 true DK2970878T3 (da) | 2018-09-03 |
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| EP (2) | EP2970878B1 (da) |
| JP (1) | JP6465857B2 (da) |
| CN (2) | CN112806352A (da) |
| CA (1) | CA2941929C (da) |
| CY (1) | CY1120801T1 (da) |
| DK (1) | DK2970878T3 (da) |
| ES (1) | ES2684582T3 (da) |
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| HU (1) | HUE039933T2 (da) |
| LT (1) | LT2970878T (da) |
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| SI (1) | SI2970878T1 (da) |
| SM (1) | SMT201800457T1 (da) |
| WO (1) | WO2014144682A1 (da) |
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| LT2970878T (lt) | 2013-03-15 | 2018-11-12 | Truckee Applied Genomics, Llc | Vėžio ląstelių chirurginiu būdu pašalintame audinyje gyvybingumo palaikymo būdai ir reagenatai |
| US10053676B2 (en) | 2014-11-25 | 2018-08-21 | Bio-Rad Laboratories, Inc. | Arginine improves polymerase storage stability |
| CN107333750A (zh) * | 2017-06-11 | 2017-11-10 | 成都吱吖科技有限公司 | 一种长时血液细胞稳定剂 |
| WO2021055170A1 (en) * | 2019-09-17 | 2021-03-25 | Longhorn Vaccines And Diagnostics, Llc | Multipurpose compositions for collecting and transporting biological material |
| WO2022010916A1 (en) * | 2020-07-07 | 2022-01-13 | Truckee Applied Genomics, Llc | Cell preservation reagents and methods of use |
| CN117063915B (zh) * | 2023-08-17 | 2026-02-24 | 镜像绮点(上海)细胞技术有限公司 | 原代肿瘤细胞冻存液 |
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| US5409826A (en) * | 1993-06-08 | 1995-04-25 | Coulter Corporation | Preserved, non-infectious control cells prepared by the modulation or modification of normal cells |
| CA2250570A1 (en) * | 1996-03-26 | 1997-10-02 | Eli Lilly And Company | Formulations of ob protein |
| US20010007673A1 (en) | 1999-11-12 | 2001-07-12 | Merrill Seymour Goldenberg | Sustained-release delayed gels |
| US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
| US20080064108A1 (en) | 1997-12-10 | 2008-03-13 | Tony Baker | Urine Preservation System |
| WO1999029904A2 (en) | 1997-12-10 | 1999-06-17 | Sierra Diagnostics, Inc. | Methods and reagents for preservation of dna in bodily fluids |
| AU1848602A (en) * | 2000-12-04 | 2002-06-18 | Lymphotec Inc. | Cell-preservation liquid and method of preserving cells by using the liquid |
| KR20020066778A (ko) | 2001-02-13 | 2002-08-21 | 한국과학기술연구원 | 체내 난흡수 물질의 흡수촉진용 조성물과 제형 및 그의제조방법 |
| WO2008111981A1 (en) | 2007-03-14 | 2008-09-18 | Sierra Molecular Corporation | Compositions, systems, and methods for preservation of macromolecules |
| WO2004052184A2 (en) * | 2002-12-12 | 2004-06-24 | Oncotech, Inc. | Genes related to sensitivity and resistance to chemotherapeutic drug treatment |
| US8793895B2 (en) * | 2006-02-10 | 2014-08-05 | Praxair Technology, Inc. | Lyophilization system and method |
| US8652782B2 (en) * | 2006-09-12 | 2014-02-18 | Longhorn Vaccines & Diagnostics, Llc | Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids |
| US8080645B2 (en) * | 2007-10-01 | 2011-12-20 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection/transport compositions and methods |
| WO2008033936A2 (en) | 2006-09-12 | 2008-03-20 | Sierra Molecular Corporation | Removal of molecular assay interferences for nucleic acids employing buffered solutions of chaotropes |
| WO2008052170A2 (en) | 2006-10-27 | 2008-05-02 | Sierra Molecular Corporation | Penetratable septum cap |
| WO2008071384A1 (en) | 2006-12-13 | 2008-06-19 | Roche Diagnostics Gmbh | Use of tde for the isolation of nucleic acids |
| KR20100015578A (ko) | 2007-03-14 | 2010-02-12 | 시에라 몰레큘러 코포레이션 | 세포 및/또는 거대분자의 보존 및/또는 안정화를 위한 조성물, 시스템 및 방법 |
| EP2217244A4 (en) * | 2007-11-12 | 2011-08-31 | Bipar Sciences Inc | TREATMENT OF NUTRITIONAL CANCER AND EGG CANCER WITH A PARP INHIBITOR ALONE OR IN COMBINATION WITH ANTITUMOROUS MEDICINES |
| US20100003748A1 (en) | 2008-07-03 | 2010-01-07 | Tony Baker | Compositions, systems, and methods for stabilization of a cell and/or macromolecule |
| WO2010028156A2 (en) | 2008-09-04 | 2010-03-11 | Board Of Regents, The University Of Texas System | Dual modality detection of apoptosis |
| US9133343B2 (en) * | 2009-11-30 | 2015-09-15 | Enzo Biochem, Inc. | Dyes and compositions, and processes for using same in analysis of protein aggregation and other applications |
| CN102318597B (zh) * | 2011-08-19 | 2013-04-03 | 张雪云 | 一种液基细胞保存液组合物及其制备方法 |
| LT2970878T (lt) | 2013-03-15 | 2018-11-12 | Truckee Applied Genomics, Llc | Vėžio ląstelių chirurginiu būdu pašalintame audinyje gyvybingumo palaikymo būdai ir reagenatai |
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