DK2976418T3 - Fremgangsmåde til fremstilling af et produkt (f.eks. polypeptid) i en kontinuerlig cellekulturfermenteringsproces - Google Patents

Fremgangsmåde til fremstilling af et produkt (f.eks. polypeptid) i en kontinuerlig cellekulturfermenteringsproces Download PDF

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Publication number
DK2976418T3
DK2976418T3 DK14708915.5T DK14708915T DK2976418T3 DK 2976418 T3 DK2976418 T3 DK 2976418T3 DK 14708915 T DK14708915 T DK 14708915T DK 2976418 T3 DK2976418 T3 DK 2976418T3
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Denmark
Prior art keywords
polypeptide
producing
product
cell culture
continuous cell
Prior art date
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DK14708915.5T
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English (en)
Inventor
Mads Laustsen
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Cmc Biologics As
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/58Reaction vessels connected in series or in parallel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/14Bioreactors or fermenters specially adapted for specific uses for producing enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02Membranes; Filters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10Separation or concentration of fermentation products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK14708915.5T 2013-03-19 2014-03-11 Fremgangsmåde til fremstilling af et produkt (f.eks. polypeptid) i en kontinuerlig cellekulturfermenteringsproces DK2976418T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13160023 2013-03-19
PCT/EP2014/054721 WO2014146933A1 (en) 2013-03-19 2014-03-11 A method for producing a product (e.g. polypeptide) in a continuous cell culture fermentation process.

Publications (1)

Publication Number Publication Date
DK2976418T3 true DK2976418T3 (da) 2021-01-11

Family

ID=47997060

Family Applications (1)

Application Number Title Priority Date Filing Date
DK14708915.5T DK2976418T3 (da) 2013-03-19 2014-03-11 Fremgangsmåde til fremstilling af et produkt (f.eks. polypeptid) i en kontinuerlig cellekulturfermenteringsproces

Country Status (5)

Country Link
US (2) US10316282B2 (da)
EP (1) EP2976418B1 (da)
DK (1) DK2976418T3 (da)
ES (1) ES2842350T3 (da)
WO (1) WO2014146933A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10202113019XA (en) * 2014-06-04 2021-12-30 Amgen Inc Methods for harvesting mammalian cell cultures
WO2016161305A1 (en) 2015-04-02 2016-10-06 Scarab Genomics, Llc Materials and methods for extended continuous flow fermentation of reduced genome bacteria
FR3056225B1 (fr) * 2016-09-21 2021-02-12 Inria Inst Nat Rech Informatique & Automatique Bioreacteur pour la selection de microalgues
WO2019156768A1 (en) * 2018-02-06 2019-08-15 Repligen Corporation Single-use process vessel with integrated filter module
CN111868249B (zh) * 2018-03-19 2024-06-18 富士胶片株式会社 生产物的制造方法
DE102018210370A1 (de) * 2018-06-26 2020-01-02 Irubis Gmbh Einweg-Bioreaktor und dessen Verwendung
WO2021143699A1 (en) * 2020-01-15 2021-07-22 Wuxi Biologics (Shanghai) Co. Ltd. An apparatus and a method for continuously harvesting a biological substance produced by a cultured cell
TWI823252B (zh) * 2022-02-09 2023-11-21 國立臺灣大學 連續型細胞生產系統及方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT370128B (de) * 1981-03-25 1983-03-10 Steyrermuehl Papier Verfahren zur gewinnung von cellulase und anlage zur durchfuehrung des verfahrens
DE3515650A1 (de) * 1985-05-02 1986-11-06 Biochemie GmbH, Kundl, Tirol Verfahren zur abtrennung von biotechnologisch hergestellten wertstoffen durch querstrom-mikrofiltration
DE3641824A1 (de) * 1986-12-06 1988-06-16 Bayer Ag Verfahren und vorrichtung zur kultivierung immobilisierter oder adhaerierter saeugerzellen sowie dessen verwendung bei der herstellung von proteinen
CA2441774A1 (en) * 2001-04-20 2002-10-31 E.I. Du Pont De Nemours And Company A product removal process for use in a biofermentation system
DE10153805A1 (de) * 2001-11-05 2003-05-15 Messo Chemietechnik Gmbh Verfahren und Vorrichtung zur Abtrennung von niedermolekularen Vergärungsprodukten aus Stoffgemischen
EP1498475A1 (en) 2003-07-18 2005-01-19 Meristem Therapeutics S.A. Continuous plant cell bioreactor and method for continuous plant cell culture
NZ591884A (en) 2004-02-23 2012-07-27 Crecy Eudes Francois Marie De Continuous culture apparatus including movable flexible tubing divided into chambers by clamps
US20050197496A1 (en) * 2004-03-04 2005-09-08 Gtc Biotherapeutics, Inc. Methods of protein fractionation using high performance tangential flow filtration
EP1720972B1 (en) 2004-03-05 2014-01-08 DSM IP Assets B.V. Process for cell culturing by continuous perfusion and alternating tangential flow
BRPI0714347A2 (pt) 2006-07-14 2013-05-07 Dsm Ip Assets Bv processo aperfeiÇoado para o cultivo de cÉlulas
DK1903115T3 (da) * 2006-09-22 2011-05-23 Wacker Chemie Ag Fremgangsmåde til fermentativ fremstilling af antistoffer
EP2014760A1 (en) * 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
EP2188371B1 (en) 2007-08-09 2017-12-20 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
US8580554B2 (en) * 2009-07-31 2013-11-12 Baxter International Inc. Method of producing a polypeptide or virus of interest in a continuous cell culture

Also Published As

Publication number Publication date
EP2976418B1 (en) 2020-09-30
US20190264154A1 (en) 2019-08-29
US10717958B2 (en) 2020-07-21
US10316282B2 (en) 2019-06-11
US20160281044A1 (en) 2016-09-29
WO2014146933A1 (en) 2014-09-25
EP2976418A1 (en) 2016-01-27
ES2842350T3 (es) 2021-07-13

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