DK3056082T3 - Genmodificerede mus og indpodning - Google Patents
Genmodificerede mus og indpodning Download PDFInfo
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- DK3056082T3 DK3056082T3 DK16157878.6T DK16157878T DK3056082T3 DK 3056082 T3 DK3056082 T3 DK 3056082T3 DK 16157878 T DK16157878 T DK 16157878T DK 3056082 T3 DK3056082 T3 DK 3056082T3
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- A01K2267/0331—Animal model for proliferative diseases
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- A—HUMAN NECESSITIES
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Claims (11)
1. Genmodificeret Rag2’/’IL-2rY’/’-mus, der omfatter en substitution af et muse-trombopoietin (TPO)-gen med et humant TPO-gen på et muse-TPO-genlocus, hvor musen omfatter humane hæmatopoietiske celler, og musen er inficeret med et humant patogen, der ikke inficerer vildtype-mus.
2. Mus ifølge krav 1, hvor musen omfatter en substitution af hvert allel af muse-TPO-genet med et humant TPO-gen.
3. Mus ifølge krav 1, yderligere omfattende en substitution af et muse-IL-3-gen med et humant IL-3-gen på et muse-IL-3-genlocus.
4. Mus ifølge krav 1 eller 3, yderligere omfattende en substitution af et muse-GM-CSF-gen med et humant GM-CSF-gen på et muse-GM-CSF-genlocus.
5. Fremgangsmåde til fremstilling af en mus omfattende humane hæmatopoietiske celler og inficeret med et humant patogen, hvilken fremgangsmåde omfatter: indpodning af humane hæmatopoietiske celler i en genmodificeret Rag2’/’IL-2ry/’-mus, hvor den genmodificerede mus omfatter en substitution af et muse-trombopoietin (TPO)-gen med et humant TPO-gen på et muse-TPO-genlocus; inficering af den genmodificerede mus med et humant patogen, der ikke inficerer vildtype-mus.
6. Fremgangsmåde ifølge krav 5, hvor musen omfatter en substitution af hvert allel af muse-TPO-genet med et humant TPO-gen.
7. Fremgangsmåde ifølge krav 5, hvor de humane hæmatopoietiske celler omfatter humane navlestrengsblodlegemer eller humane føtale leverceller.
8. Fremgangsmåde ifølge krav 5, hvor de humane hæmatopoietiske celler omfatter humane CD34+ celler.
9. Fremgangsmåde ifølge krav 5, yderligere omfattende: bestråling af den genmodificerede mus før indpodning.
10. Fremgangsmåde ifølge krav 5, hvor den genmodificerede mus yderligere omfatter en substitution af et muse-IL-3 gen med et humant IL-3-gen på et muse-IL-3-genlocus.
11. Fremgangsmåde ifølge krav 5 eller 10, hvor musen yderligere omfatter en substitution af et muse-GM-CSF-gen med et humant GM-CSF gen på et muse-GM-CSF-genested.
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| EP10822486.6A EP2485583B1 (en) | 2009-10-06 | 2010-10-04 | Genetically modified mice and engraftment |
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| DK10822486.6T DK2485583T3 (da) | 2009-10-06 | 2010-10-04 | Genmodificerede mus og indpodning |
| DK16157878.6T DK3056082T3 (da) | 2009-10-06 | 2010-10-04 | Genmodificerede mus og indpodning |
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| DK10822486.6T DK2485583T3 (da) | 2009-10-06 | 2010-10-04 | Genmodificerede mus og indpodning |
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