DK3061817T3 - Alkaliske proteasevarianter - Google Patents
Alkaliske proteasevarianter Download PDFInfo
- Publication number
- DK3061817T3 DK3061817T3 DK16163320.1T DK16163320T DK3061817T3 DK 3061817 T3 DK3061817 T3 DK 3061817T3 DK 16163320 T DK16163320 T DK 16163320T DK 3061817 T3 DK3061817 T3 DK 3061817T3
- Authority
- DK
- Denmark
- Prior art keywords
- amino acid
- dna
- oligonucleotide
- artificial
- pcr primer
- Prior art date
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- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical class OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 108010051242 phenylalanylserine Proteins 0.000 description 1
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- 229920001223 polyethylene glycol Polymers 0.000 description 1
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- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 description 1
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- 229920001296 polysiloxane Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011164 primary particle Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000006965 reversible inhibition Effects 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 108010026333 seryl-proline Proteins 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical class O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- RYMZZMVNJRMUDD-HGQWONQESA-N simvastatin Chemical compound C([C@H]1[C@@H](C)C=CC2=C[C@H](C)C[C@@H]([C@H]12)OC(=O)C(C)(C)CC)C[C@@H]1C[C@@H](O)CC(=O)O1 RYMZZMVNJRMUDD-HGQWONQESA-N 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 238000012868 site-directed mutagenesis technique Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910000031 sodium sesquicarbonate Inorganic materials 0.000 description 1
- 235000018341 sodium sesquicarbonate Nutrition 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- MWNQXXOSWHCCOZ-UHFFFAOYSA-L sodium;oxido carbonate Chemical compound [Na+].[O-]OC([O-])=O MWNQXXOSWHCCOZ-UHFFFAOYSA-L 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical compound C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 1
- 235000021286 stilbenes Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 108010075550 termamyl Proteins 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- WCTAGTRAWPDFQO-UHFFFAOYSA-K trisodium;hydrogen carbonate;carbonate Chemical compound [Na+].[Na+].[Na+].OC([O-])=O.[O-]C([O-])=O WCTAGTRAWPDFQO-UHFFFAOYSA-K 0.000 description 1
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38618—Protease or amylase in liquid compositions only
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38681—Chemically modified or immobilised enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Detergent Compositions (AREA)
Claims (11)
1. Alkalisk proteasevariant, hvor den alkaliske proteasevariant (i) består af aminosyresekvensen ifølge SEQ ID NO: 2, hvor aminosyreresten ved position 83 i aminosyresekvensen ifølge SEQ ID NO: 2 er substitueret med følgende aminosyrerester: alanin, serin eller cystein, eller (ii) har en identitet på 90% eller mere med SEQ ID NO: 2, hvor aminosyreresten ved positionen svarende til position 83 i aminosyresekvensen ifølge SEQ ID NO: 2 er substitueret med følgende aminosyrerester: alanin, serin eller cystein, og hvor den alkaliske proteasevariant udviser aktivitet i et alkalisk pH område.
2. Alkalisk proteasevariant ifølge krav 1, hvor yderligere én eller flere aminosyrerester med positioner valgt blandt (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 204, (h) position 319 og (i) position 337 i aminosyresekvensen ifølge SEQ ID NO: 2, eller ved positioner svarende dertil, er substituerede med følgende aminosyrerester: (a) tryptophan, leucin, valin, isoleucin, methionin, tyrosin, glutamin, lysin, threonin, phenylalanin, arginin, serin, cystein, alanin eller histidin; (b) glutaminsyre, methionin, aspartansyre, valin, glutamin, arginin, cystein, tryptophan, alanin eller phenylalanin; (c) methionin, glutaminsyre, arginin, valin, lysin, phenylalanin, tyrosin, isoleucin, histidin, aspartansyre eller cystein; (d) tryptophan; (e) histidin, tryptophan, serin eller leucin; (f) alanin, glutaminsyre, glutamin, serin, cystein, glycin, histidin, lysin, arginin, methionin eller asparagin; (g) glutaminsyre, aspartansyre, cystein, valin, threonin, prolin, histidin, isoleucin, tryptophan, serin, asparagin, lysin eller arginin; (h) tryptophan, valin, threonin, leucin, isoleucin, cystein, glutaminsyre, lysin, tyrosin, arginin, phenylalanin, glutamin, methionin, prolin, aspartansyre, asparagin, histidin eller serin; og (i) arginin, glycin, serin, lysin, glutamin, threonin, histidin, alanin, cystein eller valin.
3. Gen, som koder for den alkaliske proteasevariant ifølge krav 1 eller 2.
4. Rekombinant vektor omfattende genet ifølge krav 3.
5. Ikke-human transformant, omfattende den rekombinante vektor ifølge krav 4.
6. Den ikke-humane transformant ifølge krav 5, hvis vært er en mikroorganisme.
7. Vaskemiddelsammensætning omfattende den alkaliske proteasevariant ifølge krav 1 eller 2.
8. Vaskemiddelsammensætning ifølge krav 7, som omfatter et anionisk overfladeaktivt stof.
9. Fremgangsmåde til at fremme stabiliteten af en alkalisk protease i et flydende vaskemiddel, hvilken fremgangsmåde omfatter (i) i en alkalisk protease bestående af aminosyresekvensen ifølge SEQ ID NO: 2, at substituere aminosyreresten ved position 83 i aminosyresekvensen ifølge SEQ ID NO: 2 med følgende aminosyrerester: alanin, serin eller cystein; eller (ii) i en alkalisk protease med en identitet på 90% eller mere med SEQ ID NO: 2, at substituere aminosyreresten ved positionen svarende til position 83 i aminosyresekvensen ifølge SEQ ID NO: 2 med følgende aminosyrerester: alanin, serin eller cystein, hvor den fremstillede alkaliske proteasevariant udviser aktivitet i et alkalisk pH område.
10. Fremgangsmåde ifølge krav 9, hvor, i den alkaliske protease, én eller flere aminosyrerester yderligere er substituerede ved positioner valgt blandt (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 204, (h) position 319, and (i) position 337 i aminosyresekvensen ifølge SEQ ID NO: 2, eller ved positioner svarende dertil, med følgende aminosyrerester: (a) tryptophan, leucin, valin, isoleucin, methionin, tyrosin, glutamin, lysin, threonin, phenylalanin, arginin, serin, cystein, alanin eller histidin; (b) glutaminsyre, methionin, aspartansyre, valin, glutamin, arginin, cystein, tryptophan, alanin eller phenylalanin; (c) methionin, glutaminsyre, arginin, valin, lysin, phenylalanin, tyrosin, isoleucin, histidin, aspartansyre eller cystein; (d) tryptophan; (e) histidin, tryptophan, serin eller leucin; (f) alanin, glutaminsyre, glutamin, serin, cystein, glycin, histidin, lysin, arginin, methionin eller asparagin; (g) glutaminsyre, aspartansyre, cystein, valin, threonin, prolin, histidin, isoleucin, tryptophan, serin, asparagin, lysin eller arginin; (h) tryptophan, valin, threonin, leucin, isoleucin, cystein, glutaminsyre, lysin, tyrosin, arginin, phenylalanin, glutamin, methionin, prolin, aspartansyre, asparagin, histidin eller serin; og (i) arginin, glycin, serin, lysin, glutamin, threonin, histidin, alanin, cystein eller valin.
11. Fremgangsmåde til fremstilling af den alkaliske proteasevariant ifølge krav 1 eller 2, hvilken fremgangsmåde omfatter dyrkning af transformanten ifølge krav 5.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009110792 | 2009-04-30 | ||
| JP2009110793 | 2009-04-30 | ||
| JP2010093307A JP2010273672A (ja) | 2009-04-30 | 2010-04-14 | アルカリプロテアーゼ変異体 |
| JP2010093339A JP2010273673A (ja) | 2009-04-30 | 2010-04-14 | アルカリプロテアーゼ変異体 |
| EP10719131A EP2424981A2 (en) | 2009-04-30 | 2010-04-26 | Alkaline protease variants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3061817T3 true DK3061817T3 (da) | 2019-03-04 |
Family
ID=45541551
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16163320.1T DK3061817T3 (da) | 2009-04-30 | 2010-04-26 | Alkaliske proteasevarianter |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8778650B2 (da) |
| EP (2) | EP3061817B1 (da) |
| CN (1) | CN102421893B (da) |
| DK (1) | DK3061817T3 (da) |
| WO (1) | WO2010126156A2 (da) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703482B (zh) * | 2011-08-01 | 2014-08-06 | 南京工业大学 | 一种耐有机溶剂碱性蛋白酶 |
| CN103275958B (zh) * | 2011-08-01 | 2014-07-16 | 南京工业大学 | 一种耐有机溶剂碱性蛋白酶 |
| JP6067409B2 (ja) * | 2012-04-10 | 2017-01-25 | 花王株式会社 | アルカリプロテアーゼの溶解性向上方法 |
| CN103409398B (zh) * | 2013-09-03 | 2014-12-24 | 广州立白企业集团有限公司 | 一种蛋白酶及突变体及其应用 |
| JP2018521653A (ja) * | 2015-07-29 | 2018-08-09 | シージェイ チェルジェダン コーポレイション | 転換活性が高められたヘキスロン酸ｃ４−エピメラーゼ変異体及びこれを用いたｄ−タガトースの製造方法 |
| JP6289711B2 (ja) | 2016-06-09 | 2018-03-07 | 花王株式会社 | 変異アルカリプロテアーゼ |
| DK3470517T5 (da) * | 2016-06-09 | 2024-09-09 | Kao Corp | Alkalisk protease-variant |
| KR101955103B1 (ko) * | 2016-07-29 | 2019-03-06 | 씨제이제일제당 (주) | D-타가토스 전환 활성이 향상된 헥수론산 c4-에피머화 효소 변이체 및 이를 이용한 d-타가토스의 제조 방법 |
| JP7245676B2 (ja) | 2019-03-11 | 2023-03-24 | 花王株式会社 | 変異プロテアーゼ |
| EP3711772A1 (en) | 2019-03-20 | 2020-09-23 | Oslo Universitetssykehus HF | Recombinant proteins and fusion proteins |
| CN116199746B (zh) * | 2022-11-29 | 2024-08-20 | 厦门大学 | 高亲和力靶向Trop2的多元环肽分子框架 |
| KR102888405B1 (ko) * | 2023-03-27 | 2025-11-21 | 씨제이제일제당 주식회사 | 프로테아제 활성을 갖는 변이형 폴리펩티드 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0349293A (ja) | 1989-07-17 | 1991-03-04 | Toshiba Corp | 配線基板用印刷装置 |
| DK99492D0 (da) | 1992-08-07 | 1992-08-07 | Novo Nordisk As | Nyt enzym |
| JP2635288B2 (ja) | 1993-03-11 | 1997-07-30 | 花王株式会社 | 漂白剤組成物及び漂白洗浄剤組成物 |
| MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| CN1113953A (zh) * | 1994-06-01 | 1995-12-27 | 中国科学院生物物理研究所 | 枯草杆菌碱性蛋白酶第118位的突变及其热稳定酶 |
| DE69621131T2 (de) | 1995-06-13 | 2002-11-28 | Novozymes A/S, Bagsvaerd | 4-substituierte-phenylboronsäuren als enzymstabilisatoren |
| BR9712115A (pt) | 1996-09-24 | 1999-08-31 | Procter & Gamble | Detergentes líquidos contendo enzima proteolítica, aldeído peptídeo e uma fonte de ácido bórico. |
| JP3512981B2 (ja) | 1997-05-19 | 2004-03-31 | 花王株式会社 | 耐熱性アルカリセルラ−ゼ、それを生産する微生物及びその製造方法 |
| US5891701A (en) | 1997-06-12 | 1999-04-06 | Novo Nordisk Biotech Inc. | Nucleic acids encoding a polypeptide having protease activity |
| JPH1143690A (ja) | 1997-07-28 | 1999-02-16 | Kao Corp | 自動食器洗浄機用洗浄剤組成物 |
| KR100601189B1 (ko) | 1997-10-07 | 2006-07-13 | 카오 가부시키가이샤 | 알카리 프로테아제 |
| JP2002218989A (ja) | 2000-11-22 | 2002-08-06 | Kao Corp | アルカリプロテアーゼ |
| JP4143274B2 (ja) | 2001-04-12 | 2008-09-03 | 花王株式会社 | アルカリプロテアーゼ |
| US6803222B2 (en) | 2000-11-22 | 2004-10-12 | Kao Corporation | Alkaline proteases |
| JP4225721B2 (ja) | 2001-10-26 | 2009-02-18 | 花王株式会社 | アルカリプロテアーゼ |
| JP4324363B2 (ja) | 2002-06-06 | 2009-09-02 | 花王株式会社 | 変異アルカリプロテアーゼ |
| JP2004000122A (ja) | 2002-03-22 | 2004-01-08 | Kao Corp | アルカリプロテアーゼ |
| US7368273B2 (en) * | 2002-03-22 | 2008-05-06 | Kao Corporation | Alkaline protease |
| JP3872373B2 (ja) | 2002-04-18 | 2007-01-24 | 花王株式会社 | 粉末洗浄剤組成物 |
| US7996021B2 (en) | 2002-06-28 | 2011-08-09 | Hewlett-Packard Development Company, L.P. | Location determination in a wireless communication network |
| US7727756B2 (en) * | 2003-03-21 | 2010-06-01 | Novozymes A/S | Subtilases |
| JP2004305176A (ja) | 2003-04-10 | 2004-11-04 | Kao Corp | アルカリプロテアーゼ |
| JP2004305175A (ja) * | 2003-04-10 | 2004-11-04 | Kao Corp | アルカリプロテアーゼ |
| WO2006032279A1 (en) * | 2004-09-21 | 2006-03-30 | Novozymes A/S | Subtilases |
| EP2261329A3 (en) * | 2004-09-21 | 2011-01-19 | Novozymes A/S | Subtilases |
-
2010
- 2010-04-26 DK DK16163320.1T patent/DK3061817T3/da active
- 2010-04-26 EP EP16163320.1A patent/EP3061817B1/en active Active
- 2010-04-26 US US13/266,525 patent/US8778650B2/en active Active
- 2010-04-26 WO PCT/JP2010/057838 patent/WO2010126156A2/en not_active Ceased
- 2010-04-26 EP EP10719131A patent/EP2424981A2/en not_active Withdrawn
- 2010-04-26 CN CN201080019156.4A patent/CN102421893B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010126156A2 (en) | 2010-11-04 |
| CN102421893B (zh) | 2014-10-29 |
| US20120058928A1 (en) | 2012-03-08 |
| EP3061817A1 (en) | 2016-08-31 |
| EP2424981A2 (en) | 2012-03-07 |
| EP3061817B1 (en) | 2018-12-12 |
| WO2010126156A3 (en) | 2011-01-06 |
| CN102421893A (zh) | 2012-04-18 |
| US8778650B2 (en) | 2014-07-15 |
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