DK3066219T3 - Fremgangsmåder til påvisning af oligonukleotider - Google Patents
Fremgangsmåder til påvisning af oligonukleotider Download PDFInfo
- Publication number
- DK3066219T3 DK3066219T3 DK14860026.5T DK14860026T DK3066219T3 DK 3066219 T3 DK3066219 T3 DK 3066219T3 DK 14860026 T DK14860026 T DK 14860026T DK 3066219 T3 DK3066219 T3 DK 3066219T3
- Authority
- DK
- Denmark
- Prior art keywords
- certain embodiments
- oligonucleotide
- modified
- sugar
- nucleosides
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Claims (17)
1. Fremgangsmåde til påvisning eller kvantificering af et måloligonukleotid i en legemsvæske eller -ekstrakt hvor måloligonukleotidet er 14 til 40 nukleosider i længde og omfatter mindst ét modificeret nukleosid omfattende en modificeret sukkergruppe der giver øget bindingsaffinitet til en målnukleinsyre, omfattende: at danne en testprøve ved at bringe legemsvæsken eller -ekstrakt i kontakt med en indfangningsprobe, hvor: indfangningsproben er komplementær med en første del af måloligonukleotidet; og indfangningsproben omfatter en bindingsgruppe, hvor bindingsgruppen er kovalent bundet til indfangningsproben; testprøven bringes i kontakt med en fast bærer, hvor den faste bærer omfatter en bindingspartner af bindingsgruppen af indfangningsproben; testprøven bringes i kontakt med en påvisningsprobe, hvor: påvisningsproben er komplementær med en anden del af måloligonukleotidet, hvor den første del og den anden del af måloligonukleotidet ikke overlapper; og påvisningsproben omfatter en elektrochemiluminescerende gruppe, hvor den elektrochemiluminescerende gruppe er kovalent bundet til påvisningsproben; vaskning af testprøven for at fjerne ubundet probe; detektere tilstedeværelsen eller mængde af den elektrochemiluminescerende gruppe i testprøven; og derved identificere eller kvantificere måloligonukleotidet i legemsvæsken eller -ekstrakt.
2. Fremgangsmåden ifølge krav 1, hvor det mindst ene modificerede nukleosid omfattende en modificeret sukkergruppe der giver øget bindingsaffinitet til en målnukleinsyre omfatter en 2'-O-methoxyethylmodifikation.
3. Fremgangsmåden ifølge krav 1 eller 2, hvor det mindst ene modificerede nukleosid omfattende en modificeret sukkergruppe der giver øget bindingsaffinitet til en målnukleinsyre omfatter et bicyklisk nukleosid.
4. Fremgangsmåden ifølge et hvilket som helst af kravene 1-3, hvor indfangningsproben omfatter mindst én sukkermodifikation.
5. Fremgangsmåden ifølge et hvilket som helst af kravene 1-4, hvor påvisningsproben omfatter mindst én sukkermodifikation.
6. Fremgangsmåden ifølge et hvilket som helst af kravene 1-5, hvor indfangningsproben omfatter mindst én 2'-O-methoxyethyl sukkermodifikation.
7. Fremgangsmåden ifølge et hvilket som helst af kravene 1-6, hvor indfangningsproben omfatter mindst én cEt-sukkermodifikation.
8. Fremgangsmåden ifølge et hvilket som helst af kravene 1-7, hvor indfangningsproben omfatter mindst én 2'-F-sukkermodifikation.
9. Fremgangsmåden ifølge et hvilket som helst af kravene 1-8, hvor påvisningsproben omfatter mindst én 2'-O-methoxyethyl-sukkermodifikation.
10. Fremgangsmåden ifølge et hvilket som helst af kravene 1-9, hvor påvisningsproben omfatter mindst én cEt-sukkermodifikation.
11. Fremgangsmåden ifølge et hvilket som helst af kravene 1-10, hvor påvisningsproben omfatter mindst én 2'-F sukkermodifikation.
12. Fremgangsmåden ifølge et hvilket som helst af kravene 1-11, hvor legemsvæsken er cerebrospinalvæske.
13. Fremgangsmåden ifølge et hvilket som helst af kravene 1-12, hvor den elektrochemiluminescerende gruppe er tris(2,2-bipyridin) Ruthenium (II) tag.
14. Fremgangsmåden ifølge et hvilket som helst af kravene 1-13, hvor fremgangsmåden har en sensitivitet på mindre end 1 ng/ml af måloligonukleotidet.
15. Fremgangsmåden ifølge et hvilket som helst af kravene 1-14, yderligere omfattende at bringe testprøven i kontakt med en protease, eventuelt hvor proteasen er Proteinase K.
16. Fremgangsmåden ifølge et hvilket som helst af kravene 1-15, hvor indfangnings- og påvisningsproberne hver er 7-10 nukleotider i længde.
17. Fremgangsmåden ifølge et hvilket som helst af kravene 1-16, hvor måloligonukleotidet er 18-40, 20-40, 10-30, 14-30, 18-30, 20-30, 10-22, 14-22, 18-22 eller 20-22 nukleosider i længde.
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| US20160319278A1 (en) | 2015-04-03 | 2016-11-03 | University Of Massachusetts | Fully stabilized asymmetric sirna |
| WO2017030973A1 (en) | 2015-08-14 | 2017-02-23 | University Of Massachusetts | Bioactive conjugates for oligonucleotide delivery |
| US10478503B2 (en) | 2016-01-31 | 2019-11-19 | University Of Massachusetts | Branched oligonucleotides |
| CA3033368A1 (en) | 2016-08-12 | 2018-02-15 | University Of Massachusetts | Conjugated oligonucleotides |
| CA3043768A1 (en) | 2016-11-29 | 2018-06-07 | PureTech Health LLC | Exosomes for delivery of therapeutic agents |
| JP7406793B2 (ja) | 2017-06-23 | 2023-12-28 | ユニバーシティー オブ マサチューセッツ | ２テイル自己デリバリー型ｓｉＲＮＡおよび関連方法 |
| WO2020033899A1 (en) | 2018-08-10 | 2020-02-13 | University Of Massachusetts | Modified oligonucleotides targeting snps |
| US11279930B2 (en) | 2018-08-23 | 2022-03-22 | University Of Massachusetts | O-methyl rich fully stabilized oligonucleotides |
| CN113614232A (zh) | 2019-01-18 | 2021-11-05 | 马萨诸塞大学 | 动态药代动力学修饰锚 |
| KR20220047989A (ko) | 2019-08-09 | 2022-04-19 | 유니버시티 오브 매사추세츠 | Snp를 표적화하는 화학적으로 변형된 올리고뉴클레오타이드 |
| US12365894B2 (en) | 2019-09-16 | 2025-07-22 | University Of Massachusetts | Branched lipid conjugates of siRNA for specific tissue delivery |
| WO2021087164A1 (en) * | 2019-10-30 | 2021-05-06 | Shire Human Genetic Therapies, Inc. | Methods for detecting oligonucleotides |
| EP4090744A4 (en) * | 2020-01-17 | 2024-04-10 | Anastasia Khvorova | UNIVERSAL DYNAMIC PHARMACOKINETIC MODIFYING ANCHORS |
| WO2021242883A1 (en) | 2020-05-26 | 2021-12-02 | University Of Massachusetts | Synthetic oligonucleotides having regions of block and cluster modifications |
| CN117677699A (zh) | 2021-06-23 | 2024-03-08 | 马萨诸塞大学 | 用于治疗先兆子痫和其他血管生成病症的优化抗flt1寡核苷酸化合物 |
| CN118922555A (zh) * | 2021-12-30 | 2024-11-08 | 中尺度技术有限责任公司 | 用于电化学发光检测的方法 |
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-
2014
- 2014-11-10 EP EP14860026.5A patent/EP3066219B1/en active Active
- 2014-11-10 US US15/034,508 patent/US10752940B2/en active Active
- 2014-11-10 WO PCT/US2014/064874 patent/WO2015070173A1/en not_active Ceased
- 2014-11-10 DK DK14860026.5T patent/DK3066219T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3066219A4 (en) | 2017-06-14 |
| US10752940B2 (en) | 2020-08-25 |
| WO2015070173A1 (en) | 2015-05-14 |
| US20160281148A1 (en) | 2016-09-29 |
| EP3066219B1 (en) | 2018-12-26 |
| EP3066219A1 (en) | 2016-09-14 |
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