DK3068217T3 - Genmålrettet vektor for rekombinationsaktiverende gen 2, produktion af SCID-lignende miniaturesvin ved TALEN-medieret gentargeting og anvendelse deraf - Google Patents

Genmålrettet vektor for rekombinationsaktiverende gen 2, produktion af SCID-lignende miniaturesvin ved TALEN-medieret gentargeting og anvendelse deraf Download PDF

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Publication number
DK3068217T3
DK3068217T3 DK14862841.5T DK14862841T DK3068217T3 DK 3068217 T3 DK3068217 T3 DK 3068217T3 DK 14862841 T DK14862841 T DK 14862841T DK 3068217 T3 DK3068217 T3 DK 3068217T3
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Prior art keywords
gene targeting
gene
talen
scid
production
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DK14862841.5T
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DK3068217T5 (da
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Jin-Hoi Kim
Deug-Nam Kwon
Randall S Prather
Kiho Lee
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Kim Jin Hoi
Univ Missouri
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/054—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
    • A01K2267/0387—Animal model for diseases of the immune system
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8527—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic for producing animal models, e.g. for tests or diseases
    • C12N2015/8536—Animal models for genetic diseases
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C12N2506/025—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells from extra-embryonic cells, e.g. trophoblast, placenta
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  • Animal Husbandry (AREA)
  • Mycology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK14862841.5T 2013-11-13 2014-11-13 Genmålrettet vektor for rekombinationsaktiverende gen 2, produktion af SCID-lignende miniaturesvin ved TALEN-medieret gentargeting og anvendelse deraf DK3068217T5 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020130137677A KR101554166B1 (ko) 2013-11-13 2013-11-13 재조합 활성 유전자 2 유전자 적중벡터, 그 벡터가 도입된 면역세포 결핍 형질전환 미니 복제돼지 생산과 그 제조방법 및 활용
PCT/KR2014/010896 WO2015072760A1 (en) 2013-11-13 2014-11-13 Recombination activating gene 2 gene targeting vector, production of scid-like miniature pigs by talen-mediated gene targeting and use thereof

Publications (2)

Publication Number Publication Date
DK3068217T3 true DK3068217T3 (da) 2023-01-09
DK3068217T5 DK3068217T5 (da) 2024-10-14

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DK14862841.5T DK3068217T5 (da) 2013-11-13 2014-11-13 Genmålrettet vektor for rekombinationsaktiverende gen 2, produktion af SCID-lignende miniaturesvin ved TALEN-medieret gentargeting og anvendelse deraf

Country Status (9)

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US (1) US9807987B2 (da)
EP (1) EP3068217B1 (da)
JP (1) JP6216456B2 (da)
KR (1) KR101554166B1 (da)
CN (1) CN105813456B (da)
DK (1) DK3068217T5 (da)
ES (1) ES2935401T3 (da)
PL (1) PL3068217T3 (da)
WO (1) WO2015072760A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101566498B1 (ko) 2013-11-13 2015-11-06 건국대학교 산학협력단 인터루킨 2 수용체 감마 유전자 적중벡터, 그 벡터가 도입된 면역세포 결핍 형질전환 미니 복제돼지 생산과 그 제조방법 및 활용
EP3612631A4 (en) 2017-04-20 2021-01-13 Ramot at Tel-Aviv University Ltd. RECOMBINATION ACTIVATING GENE (RAG) INDUCED V (D) J GENE TARGETING
CN112522257B (zh) * 2020-09-10 2023-06-20 南京启真基因工程有限公司 用于制备rrip四个基因联合敲除的重症免疫缺陷猪源重组细胞的系统
KR102421847B1 (ko) 2020-11-03 2022-07-18 한국생명공학연구원 Jak3 유전자 돌연변이 중증복합면역결핍 동물모델 및 이의 제조방법
CN114107517B (zh) * 2021-11-10 2024-02-23 扬州大学 一种与猪流感病毒抗性相关的snp标记及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040074108A (ko) 2004-06-29 2004-08-21 황우석 지에프피 유전자가 발현되는 돼지, 또는 지티 유전자가제거된 복제돼지 및 이들의 생산방법
BR112013021785A8 (pt) * 2011-02-25 2018-07-03 Recombinetics Inc animais geneticamente modificados e métodos para fazer os mesmos
CN103255115B (zh) * 2013-04-10 2015-10-28 中国科学院广州生物医药与健康研究院 一种穿透型tat-talen蛋白及内源性基因被敲除的细胞的制备方法和应用

Also Published As

Publication number Publication date
DK3068217T5 (da) 2024-10-14
EP3068217A1 (en) 2016-09-21
JP2016536999A (ja) 2016-12-01
US20160366860A1 (en) 2016-12-22
KR101554166B1 (ko) 2015-09-22
CN105813456A (zh) 2016-07-27
ES2935401T3 (es) 2023-03-06
US9807987B2 (en) 2017-11-07
CN105813456B (zh) 2019-05-28
JP6216456B2 (ja) 2017-10-18
EP3068217A4 (en) 2017-04-26
PL3068217T3 (pl) 2023-03-13
WO2015072760A1 (en) 2015-05-21
EP3068217B1 (en) 2023-01-04
KR20150055665A (ko) 2015-05-22

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