DK3198003T3 - Podning af en adhærent celle-bioreaktor med ikke-adhærente celler øger podningsdensitetsgrænsen og reducerer nødvendig opformeringstid - Google Patents

Podning af en adhærent celle-bioreaktor med ikke-adhærente celler øger podningsdensitetsgrænsen og reducerer nødvendig opformeringstid Download PDF

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Publication number
DK3198003T3
DK3198003T3 DK15845303.5T DK15845303T DK3198003T3 DK 3198003 T3 DK3198003 T3 DK 3198003T3 DK 15845303 T DK15845303 T DK 15845303T DK 3198003 T3 DK3198003 T3 DK 3198003T3
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Denmark
Prior art keywords
scaling
adherent
propagation time
reduce required
cells increase
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DK15845303.5T
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English (en)
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Nigel Parker
Hanna P Lesch
Minna Karhinen
Minna Hassinen
Robert Shaw
Seppo Yla-Herttuala
Joonas Malinen
Eevi Lipponen
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Trizell Ltd
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Publication of DK3198003T3 publication Critical patent/DK3198003T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/21—Interferons [IFN]
    • A61K38/212—IFN-alpha
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Manufacturing & Machinery (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK15845303.5T 2014-09-29 2015-08-26 Podning af en adhærent celle-bioreaktor med ikke-adhærente celler øger podningsdensitetsgrænsen og reducerer nødvendig opformeringstid DK3198003T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1417042.7A GB201417042D0 (en) 2014-09-29 2014-09-29 Method
PCT/US2015/046927 WO2016048556A1 (en) 2014-09-25 2015-08-26 Seeding an adherent cell bioreactor with non-adherent cells increases seeding density limit and reduces required expansion time

Publications (1)

Publication Number Publication Date
DK3198003T3 true DK3198003T3 (da) 2024-09-09

Family

ID=51901197

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15845303.5T DK3198003T3 (da) 2014-09-29 2015-08-26 Podning af en adhærent celle-bioreaktor med ikke-adhærente celler øger podningsdensitetsgrænsen og reducerer nødvendig opformeringstid

Country Status (15)

Country Link
US (2) US20170051309A1 (da)
EP (1) EP3198003B1 (da)
KR (1) KR20170076679A (da)
DK (1) DK3198003T3 (da)
ES (1) ES2987311T3 (da)
FI (1) FI3198003T3 (da)
GB (1) GB201417042D0 (da)
HR (1) HRP20241176T1 (da)
HU (1) HUE068587T2 (da)
LT (1) LT3198003T (da)
PL (1) PL3198003T3 (da)
PT (1) PT3198003T (da)
RS (1) RS66028B1 (da)
SI (1) SI3198003T1 (da)
WO (1) WO2016048556A1 (da)

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WO2015148704A1 (en) 2014-03-25 2015-10-01 Terumo Bct, Inc. Passive replacement of media
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
EP3604547A1 (en) * 2016-04-14 2020-02-05 Trizell Ltd. Fixed-bed bioreactor with constant-flow pump/tubing system
CN116144500A (zh) 2016-04-14 2023-05-23 崔泽尔有限公司 具有恒流泵/管道系统的固定床生物反应器
CN109069559A (zh) * 2016-04-14 2018-12-21 崔泽尔有限公司 病毒载体稳定化
HUE055862T2 (hu) 2016-04-20 2021-12-28 Centro De Investig Energeticas Készítmények és eljárások PKLR fokozott génexpressziójára
JP6987080B2 (ja) 2016-05-05 2021-12-22 サウスウェスト リサーチ インスティテュート 細胞増殖のための三次元バイオリアクター及び関連用途
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
CN117247899A (zh) 2017-03-31 2023-12-19 泰尔茂比司特公司 细胞扩增
US10851350B1 (en) 2017-06-27 2020-12-01 Genelux Corporation Bioreactor production of virus from adherent cells
SG11202002263TA (en) 2017-10-16 2020-04-29 Centro De Investigaciones Energeticas Medioambientales Y Tecnologicas Lentiviral vectors for delivery of pklr to treat pyruvate kinase deficiency
US11149244B2 (en) 2018-04-04 2021-10-19 Southwest Research Institute Three-dimensional bioreactor for T-cell activation and expansion for immunotherapy
WO2019200167A1 (en) 2018-04-11 2019-10-17 Rocket Pharmaceuticals, Ltd. Compositions and methods for stem cell transplant
BR112021002765A2 (pt) * 2018-08-16 2021-07-20 Spacecraft Seven, Llc métodos de produção para vetores virais
JP7376576B2 (ja) 2018-09-24 2023-11-08 サウスウェスト リサーチ インスティテュート 三次元バイオリアクター
WO2021041322A1 (en) 2019-08-23 2021-03-04 Lonza Walkersville, Inc. Methods and constructs for production of lentiviral vector
US20230272418A1 (en) 2020-05-04 2023-08-31 Agc Biologics S.P.A. Lentiviral vector manufacturing process in packed bed bioreactor
EP4222250A1 (en) * 2020-10-02 2023-08-09 Genelux Corporation Bioreactor production of virus from adherent cells
AU2022377625B2 (en) 2021-10-28 2025-01-02 Southwest Research Institute Devices for cell separation
EP4619499A2 (en) * 2022-11-18 2025-09-24 Univercells Technologies SA Systems and methods for recovering target biomolecules from fixed bed bioreactors
CN116574671B (zh) * 2023-07-11 2023-10-27 苏州依科赛生物科技股份有限公司 一种hek293细胞培养基及其应用
TW202548026A (zh) 2024-05-16 2025-12-16 瑞士商輝凌國際中心公司 用於純化重組腺病毒載體之方法
WO2025238194A1 (en) 2024-05-16 2025-11-20 Ferring International Center S.A. Method for the manufacture of recombinant adenovirus vectors
WO2026013235A1 (en) 2024-07-11 2026-01-15 Ferring International Center S.A. Methods of producing recombinant adenovirus vectors
WO2026037882A2 (en) * 2024-08-14 2026-02-19 Univercells Technologies Sa Apparatus for culturing shear sensitive cells and related methods

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US4833083A (en) * 1987-05-26 1989-05-23 Sepragen Corporation Packed bed bioreactor
JPH07507921A (ja) * 1992-06-12 1995-09-07 ディーエヌエックス　コーポレーション トランスジェニックブタにおけるヒトヘモグロビンの生産
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Also Published As

Publication number Publication date
EP3198003A4 (en) 2018-08-08
EP3198003B1 (en) 2024-07-17
KR20170076679A (ko) 2017-07-04
US20170051309A1 (en) 2017-02-23
HUE068587T2 (hu) 2025-01-28
SI3198003T1 (sl) 2025-02-28
ES2987311T3 (es) 2024-11-14
RS66028B1 (sr) 2024-11-29
GB201417042D0 (en) 2014-11-12
WO2016048556A1 (en) 2016-03-31
HRP20241176T1 (hr) 2024-11-22
LT3198003T (lt) 2024-10-25
FI3198003T3 (fi) 2024-09-03
US20190276846A1 (en) 2019-09-12
EP3198003A1 (en) 2017-08-02
PT3198003T (pt) 2024-09-04
PL3198003T3 (pl) 2025-02-24

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