DK3207164T3 - Halvkontinuerlige dyrkningsfremgangsmåder - Google Patents

Halvkontinuerlige dyrkningsfremgangsmåder Download PDF

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Publication number
DK3207164T3
DK3207164T3 DK15850279.9T DK15850279T DK3207164T3 DK 3207164 T3 DK3207164 T3 DK 3207164T3 DK 15850279 T DK15850279 T DK 15850279T DK 3207164 T3 DK3207164 T3 DK 3207164T3
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DK
Denmark
Prior art keywords
semi
continuous cultivation
cultivation procedures
procedures
continuous
Prior art date
Application number
DK15850279.9T
Other languages
English (en)
Inventor
Zhiyong Sun
Michael Milway
Kevin Berryman
Mercia Valentine
Roberto E Armenta
Laura Purdue
Original Assignee
Mara Renewables Corp
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Filing date
Publication date
Application filed by Mara Renewables Corp filed Critical Mara Renewables Corp
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Publication of DK3207164T3 publication Critical patent/DK3207164T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK15850279.9T 2014-10-16 2015-10-12 Halvkontinuerlige dyrkningsfremgangsmåder DK3207164T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462064668P 2014-10-16 2014-10-16
PCT/IB2015/057807 WO2016059540A1 (en) 2014-10-16 2015-10-12 Semi-continuous culture methods

Publications (1)

Publication Number Publication Date
DK3207164T3 true DK3207164T3 (da) 2021-02-08

Family

ID=55746207

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15850279.9T DK3207164T3 (da) 2014-10-16 2015-10-12 Halvkontinuerlige dyrkningsfremgangsmåder

Country Status (16)

Country Link
US (4) US10676775B2 (da)
EP (2) EP3207164B1 (da)
JP (1) JP6806297B2 (da)
KR (1) KR102501031B1 (da)
CN (1) CN106687605B (da)
AU (3) AU2015332094B2 (da)
BR (1) BR112017003556B1 (da)
CA (1) CA2959860C (da)
CL (1) CL2017000891A1 (da)
DK (1) DK3207164T3 (da)
ES (1) ES2856838T3 (da)
IL (1) IL250275A0 (da)
MX (1) MX381412B (da)
NZ (1) NZ728653A (da)
PT (1) PT3207164T (da)
WO (1) WO2016059540A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2959860C (en) 2014-10-16 2023-04-18 MARA Renewables Corporation Semi-continuous culture methods
US10851395B2 (en) * 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
KR101956440B1 (ko) * 2017-02-10 2019-06-24 부경대학교 산학협력단 반연속 배양 방법을 이용한 바이러스의 생산 방법
CN107805649A (zh) 2017-11-10 2018-03-16 浙江帝斯曼中肯生物科技有限公司 一种生产结冷胶的新型发酵工艺
CN109371071B (zh) * 2018-05-17 2022-11-04 梁云 Dha微生物油脂中脂肪酸组合物成分调整的方法
CN109136308B (zh) * 2018-09-26 2021-01-26 武汉中科光谷绿色生物技术有限公司 一种提高发酵产聚唾液酸的方法及发酵液
CN109161570B (zh) * 2018-09-26 2020-12-18 武汉中科光谷绿色生物技术有限公司 一种提高发酵产n-乙酰神经氨酸的方法及发酵液
CN116064409A (zh) * 2023-01-06 2023-05-05 四川阿思科力生物科技有限公司 一种大规模高产量细胞连续培养和收获的生产工艺

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US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5130242A (en) * 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340594A (en) 1988-09-07 1994-08-23 Omegatech Inc. Food product having high concentrations of omega-3 highly unsaturated fatty acids
EP1707055A3 (en) 2000-01-28 2009-01-14 Martek Biosciences Corporation Enhanced production of lipids containing polyenoic fatty acids by very high density cultures of eukaryotic microbes in fermentors
US7314209B2 (en) 2004-08-06 2008-01-01 Cooper Standard Automotive, Inc. Stub out fluid quick connector with shut off valve interface
ES2909600T3 (es) 2005-06-07 2022-05-09 Dsm Nutritional Products Ag Microorganismos eucarióticos para producir lípidos y antioxidantes
US9023616B2 (en) * 2006-08-01 2015-05-05 Dsm Nutritional Products Ag Oil producing microbes and method of modification thereof
EA019387B1 (ru) * 2007-09-12 2014-03-31 Мартек Биосайнсиз Корпорейшн Способ получения биологического масла и его использование в способе получения биодизеля
AU2009206463A1 (en) * 2008-01-25 2009-07-30 Aquatic Energy Llc Algal culture production, harvesting, and processing
BRPI0917961A2 (pt) * 2008-08-04 2017-04-11 Kai Bioenergy cultivo, colheita e extração contínua de óleo de culturas fotossintéticas
CN108034680A (zh) * 2009-02-25 2018-05-15 V.B.医疗私人有限公司 发酵生产二十二碳六烯酸的改进方法
DK2580342T3 (da) * 2010-06-09 2019-07-01 Fermentalg Sammensætninger omfattende eicosapentaensyre egnet til høj oprensning
US9133463B2 (en) * 2011-03-07 2015-09-15 Dsm Nutritional Products Ag Engineering microorganisms
EP2791314A1 (en) * 2011-12-14 2014-10-22 CO.MA.SE. S.r.l. Process for production of algal biomass
CN102888348B (zh) * 2012-07-12 2014-12-10 中国科学院青岛生物能源与过程研究所 一种裂殖壶菌及利用其高密度发酵生产dha油脂的方法
CN112961785A (zh) 2014-10-16 2021-06-15 玛拉可再生能源公司 重复补料分批培养方法
CA2959860C (en) 2014-10-16 2023-04-18 MARA Renewables Corporation Semi-continuous culture methods

Also Published As

Publication number Publication date
IL250275A0 (en) 2017-03-30
KR102501031B1 (ko) 2023-02-16
AU2015332094A1 (en) 2017-02-16
US10676775B2 (en) 2020-06-09
US20220356506A1 (en) 2022-11-10
AU2023219925B2 (en) 2025-10-02
CA2959860A1 (en) 2016-04-21
CL2017000891A1 (es) 2017-11-03
JP2017530699A (ja) 2017-10-19
EP3839041A1 (en) 2021-06-23
CN106687605B (zh) 2022-02-01
EP3839041B1 (en) 2026-08-05
US20160108452A1 (en) 2016-04-21
CA2959860C (en) 2023-04-18
PT3207164T (pt) 2021-02-05
AU2021218000B2 (en) 2023-06-15
BR112017003556A2 (pt) 2018-02-27
US20200332338A1 (en) 2020-10-22
NZ728653A (en) 2023-07-28
AU2021218000A1 (en) 2021-09-02
JP6806297B2 (ja) 2021-01-06
EP3207164A1 (en) 2017-08-23
WO2016059540A1 (en) 2016-04-21
KR20170068461A (ko) 2017-06-19
MX381412B (es) 2025-03-12
EP3207164B1 (en) 2021-01-20
MX2017002234A (es) 2017-09-15
EP3207164A4 (en) 2018-04-18
BR112017003556B1 (pt) 2023-10-03
AU2023219925A1 (en) 2023-09-14
US12410458B2 (en) 2025-09-09
AU2015332094B2 (en) 2021-05-20
ES2856838T3 (es) 2021-09-28
US20250154552A1 (en) 2025-05-15
CN106687605A (zh) 2017-05-17
US11345943B2 (en) 2022-05-31

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