DK3237636T3 - Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer - Google Patents

Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer Download PDF

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Publication number
DK3237636T3
DK3237636T3 DK15820430.5T DK15820430T DK3237636T3 DK 3237636 T3 DK3237636 T3 DK 3237636T3 DK 15820430 T DK15820430 T DK 15820430T DK 3237636 T3 DK3237636 T3 DK 3237636T3
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double
nucleic acids
target nucleic
multiplex detection
quenching analysis
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DK15820430.5T
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Søren Morgenthaler Echwald
Nikolaj Dam Mikkelsen
Uffe Vest Schneider
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Anapa Biotech As
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK15820430.5T 2014-12-22 2015-12-22 Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer DK3237636T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201470813 2014-12-22
PCT/DK2015/050412 WO2016101959A1 (en) 2014-12-22 2015-12-22 Dual quenching assay for multiplex detection of target nucleic acids

Publications (1)

Publication Number Publication Date
DK3237636T3 true DK3237636T3 (da) 2021-01-25

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ID=55070628

Family Applications (1)

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DK15820430.5T DK3237636T3 (da) 2014-12-22 2015-12-22 Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer

Country Status (7)

Country Link
US (4) US20180355413A1 (da)
EP (1) EP3237636B1 (da)
JP (1) JP2018500932A (da)
CN (1) CN107109492B (da)
CA (1) CA2970966A1 (da)
DK (1) DK3237636T3 (da)
WO (1) WO2016101959A1 (da)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201501603D0 (en) 2015-01-30 2015-03-18 Norwegian University Of Life Sciences And Hedmark University College Method and product
CN108368547B (zh) 2015-12-15 2022-04-05 Seegene株式会社 与靶核酸序列有关的信号提取
US11034997B2 (en) * 2016-09-15 2021-06-15 Roche Molecular Systems, Inc. Methods for performing multiplexed real-time PCR
KR20200115552A (ko) * 2018-01-22 2020-10-07 루미넥스 코포레이션 별개의 용융 분석을 위한 방법 및 조성물
US11371080B2 (en) 2018-11-30 2022-06-28 Luminex Corporation Methods and probes for performing PCR with melt analysis for increased multiplexing
CN112592964B (zh) * 2020-12-17 2024-09-27 厦门大学 用于进行核酸多重检测的方法
WO2023043280A1 (ko) 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
US20240327899A1 (en) 2021-09-30 2024-10-03 Gen-Probe Incorporated Temperature-selectable fret cassette signaling
CN113846187B (zh) * 2021-10-24 2023-07-18 北京宏微特斯生物科技有限公司 利用荧光探针熔解曲线检测待测目标核酸序列的方法及其试剂盒
WO2024080818A1 (ko) 2022-10-14 2024-04-18 주식회사 씨젠 상이한 tm 값을 가지는 2개의 프로브를 이용하여 샘플 내 타겟 핵산을 검출하는 방법
WO2024106890A1 (ko) * 2022-11-16 2024-05-23 주식회사 씨젠 퀀칭-pto 절단 및 연장(quenching-pto cleavage and extension; q-ptoce) 어세이에 의한 타겟 핵산의 검출
WO2024234030A1 (en) * 2023-05-15 2024-11-21 Speedx Pty Ltd Target detection using temperature controlled probes
CZ2024298A3 (cs) * 2024-08-02 2026-03-11 Hola Biolabs s.r.o. Detekce alespoň jedné cílové sekvence nukleové kyseliny a kit pro detekci

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US6582908B2 (en) * 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
DE69734576T2 (de) * 1996-03-01 2006-08-03 E.I. Dupont De Nemours And Co., Wilmington Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
AU9063398A (en) 1997-09-12 1999-04-05 Exiqon A/S Oligonucleotide analogues
US7824859B2 (en) * 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7534568B2 (en) * 1999-10-29 2009-05-19 Hologic Inc. Methods for detection of a target nucleic acid by forming a cleavage structure with a cleavage resistant probe
US7585632B2 (en) * 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US7829313B2 (en) * 2000-03-24 2010-11-09 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
EP1509624A2 (en) * 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands
US7582470B2 (en) * 2002-07-31 2009-09-01 Gen-Probe Incorporated Device for amplifying and detecting a target nucleic acid
US20070092880A1 (en) * 2003-07-16 2007-04-26 Crothers Donald M Invasive cleavage reaction with electrochemical readout
AU2006251496B2 (en) 2005-05-25 2012-11-22 Tina Holding Aps Stable and selective formation of hoogsteen-type triplexes and duplexes using twisted intercalating nucleic acids (TINA) and process for the preparation of TINA
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
EP2014774A1 (en) 2007-07-11 2009-01-14 Pathofinder B.V. Assay for the simulataneous detection of multiple nucleic acid sequences in a sample
SG10201605049QA (en) * 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
HRP20181564T1 (hr) * 2011-06-30 2018-11-30 Arrowhead Pharmaceuticals, Inc. Pripravci i postupci za inhibiciju ekspresije gena virusa hepatitisa b
KR102041832B1 (ko) * 2011-08-04 2019-11-08 도레이 카부시키가이샤 췌장암의 치료 및/또는 예방용 의약 조성물
GB201116131D0 (en) * 2011-09-19 2011-11-02 Epistem Ltd Probe
JP6045500B2 (ja) * 2011-10-13 2016-12-14 国立研究開発法人農業・食品産業技術総合研究機構 チトクロームp450をコードする遺伝子及びその利用
CN103975061A (zh) * 2011-10-31 2014-08-06 荣研化学株式会社 靶核酸的检测方法
FR2983005B1 (fr) * 2011-11-18 2015-08-07 Airbus Operations Sas Dispositif d'alimentation a decoupage et aeronef comprenant au moins un tel dispositif
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Pto 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
JP5976849B2 (ja) * 2012-03-05 2016-08-24 シージーン アイエヌシー PTO切断及び伸長アッセイによるターゲット核酸配列でのヌクレオチド変異検出{DetectionofNucleotideVariationonTargetNucleicAcidSequencebyPTOCleavageandExtensionAssay}
US20140038182A1 (en) * 2012-07-17 2014-02-06 Dna Logix, Inc. Cooperative primers, probes, and applications thereof
US20140215653A1 (en) * 2012-10-24 2014-07-31 Targeted Growth, Inc. Identification and use of early embryo and/or early endosperm specific promoters for gene expression in maize
EP2971147A4 (en) * 2013-03-15 2016-08-31 Abbott Molecular Inc MULTIPLEX ALLELNACHWEIS
JP6967006B2 (ja) * 2016-01-15 2021-11-17 クアンタポール, インコーポレイテッド 低減されたバックグラウンドの光学に基づくナノポア分析

Also Published As

Publication number Publication date
EP3237636A1 (en) 2017-11-01
JP2018500932A (ja) 2018-01-18
CA2970966A1 (en) 2016-06-30
US20240344114A1 (en) 2024-10-17
WO2016101959A1 (en) 2016-06-30
US20240336955A1 (en) 2024-10-10
US20210189468A1 (en) 2021-06-24
US20180355413A1 (en) 2018-12-13
CN107109492A (zh) 2017-08-29
CN107109492B (zh) 2021-02-26
EP3237636B1 (en) 2020-10-21

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