DK3237636T3 - Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer - Google Patents

Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer Download PDF

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Publication number
DK3237636T3
DK3237636T3 DK15820430.5T DK15820430T DK3237636T3 DK 3237636 T3 DK3237636 T3 DK 3237636T3 DK 15820430 T DK15820430 T DK 15820430T DK 3237636 T3 DK3237636 T3 DK 3237636T3
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Prior art keywords
double
nucleic acids
target nucleic
multiplex detection
quenching analysis
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DK15820430.5T
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English (en)
Inventor
Søren Morgenthaler Echwald
Nikolaj Dam Mikkelsen
Uffe Vest Schneider
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Anapa Biotech As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK15820430.5T 2014-12-22 2015-12-22 Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer DK3237636T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201470813 2014-12-22
PCT/DK2015/050412 WO2016101959A1 (en) 2014-12-22 2015-12-22 Dual quenching assay for multiplex detection of target nucleic acids

Publications (1)

Publication Number Publication Date
DK3237636T3 true DK3237636T3 (da) 2021-01-25

Family

ID=55070628

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15820430.5T DK3237636T3 (da) 2014-12-22 2015-12-22 Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer

Country Status (7)

Country Link
US (4) US20180355413A1 (da)
EP (1) EP3237636B1 (da)
JP (1) JP2018500932A (da)
CN (1) CN107109492B (da)
CA (1) CA2970966A1 (da)
DK (1) DK3237636T3 (da)
WO (1) WO2016101959A1 (da)

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EP3743211A4 (en) * 2018-01-22 2021-11-24 Luminex Corporation METHODS AND COMPOSITIONS FOR THE ANALYSIS OF DISCRETE MELT
US11371080B2 (en) 2018-11-30 2022-06-28 Luminex Corporation Methods and probes for performing PCR with melt analysis for increased multiplexing
CN112592964B (zh) * 2020-12-17 2024-09-27 厦门大学 用于进行核酸多重检测的方法
KR20240058112A (ko) 2021-09-17 2024-05-03 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
EP4700133A3 (en) 2021-09-30 2026-04-29 Gen-Probe Incorporated Temperature-selectable fret cassette signaling
CN113846187B (zh) * 2021-10-24 2023-07-18 北京宏微特斯生物科技有限公司 利用荧光探针熔解曲线检测待测目标核酸序列的方法及其试剂盒
KR20250086619A (ko) 2022-10-14 2025-06-13 주식회사 씨젠 상이한 tm 값을 가지는 2개의 프로브를 이용하여 샘플 내 타겟 핵산을 검출하는 방법
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Also Published As

Publication number Publication date
WO2016101959A1 (en) 2016-06-30
EP3237636A1 (en) 2017-11-01
JP2018500932A (ja) 2018-01-18
US20240336955A1 (en) 2024-10-10
EP3237636B1 (en) 2020-10-21
US20180355413A1 (en) 2018-12-13
CN107109492B (zh) 2021-02-26
US20210189468A1 (en) 2021-06-24
CA2970966A1 (en) 2016-06-30
US20240344114A1 (en) 2024-10-17
CN107109492A (zh) 2017-08-29

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