DK3262182T3 - Modificeret creatinase - Google Patents

Modificeret creatinase Download PDF

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Publication number
DK3262182T3
DK3262182T3 DK16705950.0T DK16705950T DK3262182T3 DK 3262182 T3 DK3262182 T3 DK 3262182T3 DK 16705950 T DK16705950 T DK 16705950T DK 3262182 T3 DK3262182 T3 DK 3262182T3
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DK
Denmark
Prior art keywords
creatinase
modified
modified creatinase
Prior art date
Application number
DK16705950.0T
Other languages
English (en)
Inventor
Palle Schneider
Thomas Steen Hansen
Jens Østergaard
Original Assignee
Radiometer Medical Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Radiometer Medical Aps filed Critical Radiometer Medical Aps
Application granted granted Critical
Publication of DK3262182T3 publication Critical patent/DK3262182T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • C12N9/0034—Sarcosine oxidase (1.5.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/03—Oxidoreductases acting on the CH-NH group of donors (1.5) with oxygen as acceptor (1.5.3)
    • C12Y105/03001—Sarcosine oxidase (1.5.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/0201—Creatininase (3.5.2.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/03—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amidines (3.5.3)
    • C12Y305/03003—Creatinase (3.5.3.3), i.e. creatine amidinohydrolase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK16705950.0T 2015-02-27 2016-02-23 Modificeret creatinase DK3262182T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201500114 2015-02-27
PCT/EP2016/053760 WO2016135136A1 (en) 2015-02-27 2016-02-23 Modified creatinase

Publications (1)

Publication Number Publication Date
DK3262182T3 true DK3262182T3 (da) 2020-03-16

Family

ID=55409841

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16705950.0T DK3262182T3 (da) 2015-02-27 2016-02-23 Modificeret creatinase

Country Status (6)

Country Link
US (1) US10597695B2 (da)
EP (1) EP3262182B1 (da)
JP (1) JP6636533B2 (da)
CN (2) CN107257855B (da)
DK (1) DK3262182T3 (da)
WO (1) WO2016135136A1 (da)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10597695B2 (en) * 2015-02-27 2020-03-24 Radiometer Medical Aps Modified creatinase
CN106544292B (zh) * 2016-09-27 2019-08-23 广东省生态环境技术研究所 一株耐盐硝酸盐还原菌及其应用
EP3661420B1 (en) 2017-07-31 2023-08-23 Siemens Healthcare Diagnostics Inc. Scavenger protein(s) for improved preservation of analyte detection sensor(s) and method(s) of use thereof
GB201712592D0 (en) * 2017-08-04 2017-09-20 Imp Innovations Ltd Novel compositions and uses thereof
CN110029094B (zh) * 2019-03-01 2020-11-17 武汉生之源生物科技股份有限公司 一种突变的肌氨酸氧化酶及其在肌酐检测中的应用
US20220256849A1 (en) * 2019-07-31 2022-08-18 Siemens Healthcare Diagnostics Inc. Improved biocide formulations for the preservation of analyte detection sensor(s) and method(s) of use and thereof
US12139741B2 (en) 2019-10-25 2024-11-12 Instrumentation Laboratory Company Biocide compositions compatible with enzyme biosensors and methods of use thereof
CN116716281B (zh) * 2020-08-28 2024-10-29 上海瀚诺威生物科技有限公司 一种热稳定性提高的肌酸脒基水解酶突变体
CN111979217A (zh) * 2020-08-28 2020-11-24 上海瀚诺威生物科技有限公司 一种低米氏常数的肌酸脒基水解酶及其制备方法
CN112011529B (zh) * 2020-08-28 2022-11-29 上海瀚诺威生物科技有限公司 一种活性提高的肌酸脒基水解酶突变体
AU2024316973A1 (en) * 2023-07-31 2026-01-22 Abbott Diabetes Care Inc. Background interference mitigation for high sensitivity creatinine sensing
CN117448306B (zh) * 2023-10-27 2025-10-31 上海天鹜科技有限公司 一种应用于肌酐检测的肌酸脒基水解酶突变体及其突变位点选择、纯化和检测方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3325128B2 (ja) * 1994-09-29 2002-09-17 キッコーマン株式会社 新規なクレアチンアミジノハイドロラーゼ遺伝子、新規な組み換え体ｄｎａ及びクレアチンアミジノハイドロラーゼの製造法
JP3075390B2 (ja) * 1996-02-13 2000-08-14 東洋紡績株式会社 新規なクレアチンアミジノヒドロラーゼ、その製法およびその用途
JP3422197B2 (ja) * 1996-12-17 2003-06-30 東洋紡績株式会社 安定なクレアチンアミジノヒドロラーゼ
JP2000157279A (ja) * 1998-11-25 2000-06-13 Kikkoman Corp クレアチンアミジノハイドロラーゼ及びその製造法
CA2404293C (en) * 2001-09-20 2007-05-15 F. Hoffmann-La Roche Ag Variants of an erwinia-type creatinase
WO2006042001A2 (en) * 2004-10-05 2006-04-20 Bayer Healthcare Llc Stable three enzyme creatinine biosensor
CN102375066B (zh) * 2011-09-20 2013-10-16 天津希恩思生化科技有限公司 一种肌酸酐含量检测试剂和试剂盒，及试剂盒的制作和使用方法
US10597695B2 (en) * 2015-02-27 2020-03-24 Radiometer Medical Aps Modified creatinase

Also Published As

Publication number Publication date
US20180340207A1 (en) 2018-11-29
EP3262182A1 (en) 2018-01-03
CN114958814A (zh) 2022-08-30
US10597695B2 (en) 2020-03-24
JP2018509142A (ja) 2018-04-05
CN107257855B (zh) 2022-04-29
CN107257855A (zh) 2017-10-17
EP3262182B1 (en) 2019-12-11
WO2016135136A1 (en) 2016-09-01
JP6636533B2 (ja) 2020-01-29

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