DK3354746T3 - Nye spike-in-nukleotider til normalisering af sekvensdata - Google Patents
Nye spike-in-nukleotider til normalisering af sekvensdata Download PDFInfo
- Publication number
- DK3354746T3 DK3354746T3 DK17153689.9T DK17153689T DK3354746T3 DK 3354746 T3 DK3354746 T3 DK 3354746T3 DK 17153689 T DK17153689 T DK 17153689T DK 3354746 T3 DK3354746 T3 DK 3354746T3
- Authority
- DK
- Denmark
- Prior art keywords
- nucleotides
- sequence data
- normalizing sequence
- new spike
- spike
- Prior art date
Links
- 239000002773 nucleotide Substances 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/207—Modifications characterised by siRNA, miRNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2545/00—Reactions characterised by their quantitative nature
- C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
- C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17153689.9A EP3354746B1 (en) | 2017-01-30 | 2017-01-30 | Novel spike-in oligonucleotides for normalization of sequence data |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3354746T3 true DK3354746T3 (da) | 2019-09-02 |
Family
ID=57965671
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17153689.9T DK3354746T3 (da) | 2017-01-30 | 2017-01-30 | Nye spike-in-nukleotider til normalisering af sekvensdata |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20200040393A1 (da) |
| EP (1) | EP3354746B1 (da) |
| JP (1) | JP7044270B2 (da) |
| CN (1) | CN110446788B (da) |
| CA (1) | CA3051720A1 (da) |
| DK (1) | DK3354746T3 (da) |
| WO (1) | WO2018138334A1 (da) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020041449A1 (en) * | 2018-08-21 | 2020-02-27 | Zymo Research Corporation | Methods and compositions for tracking sample quality |
| US20230304084A1 (en) * | 2019-02-28 | 2023-09-28 | Inivata Ltd. | Method for quantifying the amount of a target sequence in a sample |
| CN111235244A (zh) * | 2019-11-28 | 2020-06-05 | 广州微远基因科技有限公司 | 测序内标分子及其制备方法和应用 |
| EP3836148A1 (en) * | 2019-12-09 | 2021-06-16 | Lexogen GmbH | Index sequences for multiplex parallel sequencing |
| CN111607639B (zh) * | 2020-05-08 | 2024-10-18 | 深圳华大因源医药科技有限公司 | 基于内参进行宏基因组病原定量检测的方法和装置 |
| WO2022029449A1 (en) * | 2020-08-07 | 2022-02-10 | Oxford Nanopore Technologies Limited | Methods of identifying nucleic acid barcodes |
| WO2024017613A1 (en) * | 2022-07-20 | 2024-01-25 | Hummingbird Diagnostics Gmbh | Artificial small rna spike-in cocktail for process control and normalization |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5637459A (en) * | 1990-06-11 | 1997-06-10 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: chimeric selex |
| JP3102800B2 (ja) | 1994-08-19 | 2000-10-23 | パーキン−エルマー コーポレイション | 増幅及び連結反応の共役法 |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| AU7537200A (en) | 1999-09-29 | 2001-04-30 | Solexa Ltd. | Polynucleotide sequencing |
| CN101525660A (zh) | 2000-07-07 | 2009-09-09 | 维西根生物技术公司 | 实时序列测定 |
| EP1394251A1 (en) * | 2002-08-23 | 2004-03-03 | Direvo Biotech AG | Method for the selective randomization of polynucleotides |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| US7170050B2 (en) | 2004-09-17 | 2007-01-30 | Pacific Biosciences Of California, Inc. | Apparatus and methods for optical analysis of molecules |
| JP2008513782A (ja) | 2004-09-17 | 2008-05-01 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | 分子解析のための装置及び方法 |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| US20070130653A1 (en) * | 2005-06-17 | 2007-06-07 | Pioneer Hi-Bred International, Inc. | Methods and compositions for gene silencing |
| US7282337B1 (en) | 2006-04-14 | 2007-10-16 | Helicos Biosciences Corporation | Methods for increasing accuracy of nucleic acid sequencing |
| WO2008040355A2 (en) * | 2006-10-06 | 2008-04-10 | Exiqon A/S | Novel methods for quantification of micrornas and small interfering rnas |
| JP2013527769A (ja) | 2010-05-06 | 2013-07-04 | アイビス バイオサイエンシズ インコーポレイティッド | 統合された試料調製システムおよび安定化酵素混合物 |
| GB201113214D0 (en) * | 2011-07-29 | 2011-09-14 | Univ East Anglia | Analysing sequencing bias |
| US10155941B2 (en) | 2012-08-01 | 2018-12-18 | Bernhard Suter | High throughput yeast two-hybrid screening method and reagent kit |
| CA3043574C (en) * | 2012-11-26 | 2023-09-19 | The University Of Toledo | Methods for standardized sequencing of nucleic acids and uses thereof |
| GB201402249D0 (en) * | 2014-02-10 | 2014-03-26 | Vela Operations Pte Ltd | NGS systems control and methods involving the same |
| GB201411603D0 (en) * | 2014-06-30 | 2014-08-13 | Vela Operations Pte Ltd | Compositions for quantitative and/or semiquantitative mutation detection methods |
| WO2016007951A1 (en) * | 2014-07-11 | 2016-01-14 | KARNAKIS, Jennifer A. | Compositions and methods for detecting rare sequence variants in nucleic acid sequencing |
| JP6705171B2 (ja) * | 2014-11-26 | 2020-06-03 | 東レ株式会社 | 小型ｒｎａの発現量の補正方法及び装置 |
| CN105648078A (zh) * | 2016-03-01 | 2016-06-08 | 浙江农林大学 | 一种山核桃嫁接过程中小rna测序分析方法 |
-
2017
- 2017-01-30 DK DK17153689.9T patent/DK3354746T3/da active
- 2017-01-30 EP EP17153689.9A patent/EP3354746B1/en active Active
-
2018
- 2018-01-29 WO PCT/EP2018/052127 patent/WO2018138334A1/en not_active Ceased
- 2018-01-29 CN CN201880012023.0A patent/CN110446788B/zh active Active
- 2018-01-29 US US16/481,283 patent/US20200040393A1/en active Pending
- 2018-01-29 CA CA3051720A patent/CA3051720A1/en active Pending
- 2018-01-29 JP JP2019561352A patent/JP7044270B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110446788B (zh) | 2024-02-23 |
| WO2018138334A1 (en) | 2018-08-02 |
| EP3354746A1 (en) | 2018-08-01 |
| CN110446788A (zh) | 2019-11-12 |
| CA3051720A1 (en) | 2018-08-02 |
| EP3354746B1 (en) | 2019-05-29 |
| US20200040393A1 (en) | 2020-02-06 |
| JP2020519256A (ja) | 2020-07-02 |
| JP7044270B2 (ja) | 2022-03-30 |
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