DK3407733T3 - Fremgangsmåde til ekstracellulær udskillelse af brazzein - Google Patents

Fremgangsmåde til ekstracellulær udskillelse af brazzein Download PDF

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Publication number
DK3407733T3
DK3407733T3 DK17743868.6T DK17743868T DK3407733T3 DK 3407733 T3 DK3407733 T3 DK 3407733T3 DK 17743868 T DK17743868 T DK 17743868T DK 3407733 T3 DK3407733 T3 DK 3407733T3
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DK
Denmark
Prior art keywords
brazzine
extra
procedure
cellular separation
cellular
Prior art date
Application number
DK17743868.6T
Other languages
English (en)
Inventor
Abhiram Dukkipati
Aakruthi Vasihnavi Balagangadhar
Original Assignee
Magellan Life Sciences Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Magellan Life Sciences Ltd filed Critical Magellan Life Sciences Ltd
Application granted granted Critical
Publication of DK3407733T3 publication Critical patent/DK3407733T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • A23L27/31Artificial sweetening agents containing amino acids, nucleotides, peptides or derivatives
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43Sweetening agents, e.g. thaumatin, monellin

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DK17743868.6T 2016-01-27 2017-01-27 Fremgangsmåde til ekstracellulær udskillelse af brazzein DK3407733T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN201641002922 2016-01-27
PCT/IN2017/050039 WO2017130222A1 (en) 2016-01-27 2017-01-27 Process for extracellular secretion of brazzein

Publications (1)

Publication Number Publication Date
DK3407733T3 true DK3407733T3 (da) 2022-01-24

Family

ID=59397630

Family Applications (1)

Application Number Title Priority Date Filing Date
DK17743868.6T DK3407733T3 (da) 2016-01-27 2017-01-27 Fremgangsmåde til ekstracellulær udskillelse af brazzein

Country Status (6)

Country Link
US (2) US11613757B2 (da)
EP (1) EP3407733B1 (da)
CN (1) CN109310119A (da)
DK (1) DK3407733T3 (da)
ES (1) ES2904642T3 (da)
WO (1) WO2017130222A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017130222A1 (en) * 2016-01-27 2017-08-03 Magellan Life Sciences Private Limited Process for extracellular secretion of brazzein
CN112300256A (zh) * 2020-10-22 2021-02-02 珠海中科先进技术研究院有限公司 一种神秘果素重组蛋白及其表达与纯化方法
CN114196699B (zh) * 2021-12-02 2022-12-20 猎境(嘉兴)生物科技有限公司 一种增加植物细胞重组蛋白胞外分泌的方法
CN116042502B (zh) * 2023-02-27 2026-02-06 南京工业大学 一种胞外分泌贻贝蛋白的基因工程菌及其构建方法与应用
WO2025147505A1 (en) * 2024-01-04 2025-07-10 Oobli, Inc. Compositions and methods for stabilized brazzein polypeptides

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6592876B1 (en) * 1993-04-20 2003-07-15 Uab Research Foundation Pneumococcal genes, portions thereof, expression products therefrom, and uses of such genes, portions and products
US6274707B1 (en) * 1999-04-14 2001-08-14 Wisconsin Alumni Research Foundation Protein sweetener
US20030219854A1 (en) * 2002-03-21 2003-11-27 Micrologix Biotech Inc. Methods for producing modified anti-infective peptides
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
CN101570754A (zh) * 2008-10-31 2009-11-04 刘松财 一种高甜度的甜味蛋白基因及合成方法
US8592181B2 (en) * 2009-08-28 2013-11-26 Chung-Ang University Industry-Academy Cooperation Foundation Brazzein variant having higher sweetness and method for preparing multi-variant
WO2012127002A1 (en) * 2011-03-23 2012-09-27 Novozymes A/S Sweet-tasting polypeptide from gram-positive bacteria
WO2017130222A1 (en) * 2016-01-27 2017-08-03 Magellan Life Sciences Private Limited Process for extracellular secretion of brazzein

Also Published As

Publication number Publication date
WO2017130222A1 (en) 2017-08-03
CN109310119A (zh) 2019-02-05
EP3407733A4 (en) 2019-07-03
EP3407733A1 (en) 2018-12-05
US20190153455A1 (en) 2019-05-23
ES2904642T3 (es) 2022-04-05
US11613757B2 (en) 2023-03-28
EP3407733B1 (en) 2021-10-20
US20230235340A1 (en) 2023-07-27
WO2017130222A9 (en) 2017-09-08
US12606836B2 (en) 2026-04-21

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