DK3407733T3 - Fremgangsmåde til ekstracellulær udskillelse af brazzein - Google Patents
Fremgangsmåde til ekstracellulær udskillelse af brazzein Download PDFInfo
- Publication number
- DK3407733T3 DK3407733T3 DK17743868.6T DK17743868T DK3407733T3 DK 3407733 T3 DK3407733 T3 DK 3407733T3 DK 17743868 T DK17743868 T DK 17743868T DK 3407733 T3 DK3407733 T3 DK 3407733T3
- Authority
- DK
- Denmark
- Prior art keywords
- brazzine
- extra
- procedure
- cellular separation
- cellular
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/30—Artificial sweetening agents
- A23L27/31—Artificial sweetening agents containing amino acids, nucleotides, peptides or derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C07K14/43—Sweetening agents, e.g. thaumatin, monellin
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN201641002922 | 2016-01-27 | ||
| PCT/IN2017/050039 WO2017130222A1 (en) | 2016-01-27 | 2017-01-27 | Process for extracellular secretion of brazzein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3407733T3 true DK3407733T3 (da) | 2022-01-24 |
Family
ID=59397630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17743868.6T DK3407733T3 (da) | 2016-01-27 | 2017-01-27 | Fremgangsmåde til ekstracellulær udskillelse af brazzein |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US11613757B2 (da) |
| EP (1) | EP3407733B1 (da) |
| CN (1) | CN109310119A (da) |
| DK (1) | DK3407733T3 (da) |
| ES (1) | ES2904642T3 (da) |
| WO (1) | WO2017130222A1 (da) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017130222A1 (en) * | 2016-01-27 | 2017-08-03 | Magellan Life Sciences Private Limited | Process for extracellular secretion of brazzein |
| CN112300256A (zh) * | 2020-10-22 | 2021-02-02 | 珠海中科先进技术研究院有限公司 | 一种神秘果素重组蛋白及其表达与纯化方法 |
| CN114196699B (zh) * | 2021-12-02 | 2022-12-20 | 猎境(嘉兴)生物科技有限公司 | 一种增加植物细胞重组蛋白胞外分泌的方法 |
| CN116042502B (zh) * | 2023-02-27 | 2026-02-06 | 南京工业大学 | 一种胞外分泌贻贝蛋白的基因工程菌及其构建方法与应用 |
| WO2025147505A1 (en) * | 2024-01-04 | 2025-07-10 | Oobli, Inc. | Compositions and methods for stabilized brazzein polypeptides |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6592876B1 (en) * | 1993-04-20 | 2003-07-15 | Uab Research Foundation | Pneumococcal genes, portions thereof, expression products therefrom, and uses of such genes, portions and products |
| US6274707B1 (en) * | 1999-04-14 | 2001-08-14 | Wisconsin Alumni Research Foundation | Protein sweetener |
| US20030219854A1 (en) * | 2002-03-21 | 2003-11-27 | Micrologix Biotech Inc. | Methods for producing modified anti-infective peptides |
| US20070059713A1 (en) * | 2005-09-09 | 2007-03-15 | Lee Jun E | SSB-DNA polymerase fusion proteins |
| CN101570754A (zh) * | 2008-10-31 | 2009-11-04 | 刘松财 | 一种高甜度的甜味蛋白基因及合成方法 |
| US8592181B2 (en) * | 2009-08-28 | 2013-11-26 | Chung-Ang University Industry-Academy Cooperation Foundation | Brazzein variant having higher sweetness and method for preparing multi-variant |
| WO2012127002A1 (en) * | 2011-03-23 | 2012-09-27 | Novozymes A/S | Sweet-tasting polypeptide from gram-positive bacteria |
| WO2017130222A1 (en) * | 2016-01-27 | 2017-08-03 | Magellan Life Sciences Private Limited | Process for extracellular secretion of brazzein |
-
2017
- 2017-01-27 WO PCT/IN2017/050039 patent/WO2017130222A1/en not_active Ceased
- 2017-01-27 EP EP17743868.6A patent/EP3407733B1/en active Active
- 2017-01-27 US US16/073,702 patent/US11613757B2/en active Active
- 2017-01-27 ES ES17743868T patent/ES2904642T3/es active Active
- 2017-01-27 DK DK17743868.6T patent/DK3407733T3/da active
- 2017-01-27 CN CN201780020439.2A patent/CN109310119A/zh active Pending
-
2023
- 2023-02-14 US US18/109,759 patent/US12606836B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017130222A1 (en) | 2017-08-03 |
| CN109310119A (zh) | 2019-02-05 |
| EP3407733A4 (en) | 2019-07-03 |
| EP3407733A1 (en) | 2018-12-05 |
| US20190153455A1 (en) | 2019-05-23 |
| ES2904642T3 (es) | 2022-04-05 |
| US11613757B2 (en) | 2023-03-28 |
| EP3407733B1 (en) | 2021-10-20 |
| US20230235340A1 (en) | 2023-07-27 |
| WO2017130222A9 (en) | 2017-09-08 |
| US12606836B2 (en) | 2026-04-21 |
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