DK34191A - STEPS IN SITU HYBRIDIZATION ANALYSIS - Google Patents

STEPS IN SITU HYBRIDIZATION ANALYSIS Download PDF

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Publication number
DK34191A
DK34191A DK034191A DK34191A DK34191A DK 34191 A DK34191 A DK 34191A DK 034191 A DK034191 A DK 034191A DK 34191 A DK34191 A DK 34191A DK 34191 A DK34191 A DK 34191A
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DK
Denmark
Prior art keywords
situ hybridization
steps
hybridization analysis
assay
copies
Prior art date
Application number
DK034191A
Other languages
Danish (da)
Other versions
DK34191D0 (en
Inventor
Mary Jean Evinger-Hodges
Joel Bresser
Original Assignee
Res Dev Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Dev Foundation filed Critical Res Dev Foundation
Publication of DK34191D0 publication Critical patent/DK34191D0/en
Publication of DK34191A publication Critical patent/DK34191A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • AIDS & HIV (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Inorganic Insulating Materials (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Television Systems (AREA)
  • Stereo-Broadcasting Methods (AREA)
  • Valve Device For Special Equipments (AREA)

Abstract

A quantitative, sensitive, One-Step In Situ hybridization assay is provided which will detect as few as 1-5 copies of target biopolymer per cell and may be accomplished in 5 minutes to 4 hours. There is provided a simultaneous assay for detecting multiple biopolymers within the same cell.
DK034191A 1988-08-31 1991-02-27 STEPS IN SITU HYBRIDIZATION ANALYSIS DK34191A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23910688A 1988-08-31 1988-08-31
PCT/US1989/003580 WO1990002173A1 (en) 1988-08-31 1989-08-18 One-step in situ hybridization assay

Publications (2)

Publication Number Publication Date
DK34191D0 DK34191D0 (en) 1991-02-27
DK34191A true DK34191A (en) 1991-04-30

Family

ID=22900643

Family Applications (1)

Application Number Title Priority Date Filing Date
DK034191A DK34191A (en) 1988-08-31 1991-02-27 STEPS IN SITU HYBRIDIZATION ANALYSIS

Country Status (12)

Country Link
EP (2) EP0432221B1 (en)
JP (1) JPH04502552A (en)
AT (1) ATE153705T1 (en)
AU (1) AU636854B2 (en)
DE (1) DE68928080T2 (en)
DK (1) DK34191A (en)
FI (1) FI910957A7 (en)
IL (1) IL91405A (en)
NZ (1) NZ230389A (en)
PT (1) PT91589B (en)
WO (1) WO1990002173A1 (en)
ZA (1) ZA896490B (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5985549A (en) * 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
WO1990002173A1 (en) * 1988-08-31 1990-03-08 Research Development Foundation One-step in situ hybridization assay
US6242184B1 (en) 1988-10-18 2001-06-05 University Of Massachusetts In-situ hybridization of single-copy and multiple-copy nucleic acid sequences
US6203977B1 (en) 1988-11-15 2001-03-20 Yale University Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization
JPH04505556A (en) * 1989-03-07 1992-10-01 シンジェン，インコーポレイテッド In situ hybridization in suspension for cell detection or isolation
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
IL99025A0 (en) * 1990-08-15 1992-07-15 Astra Ab Novel process for the detection of pathogens using dna probes
US5521061A (en) * 1992-07-17 1996-05-28 Aprogenex, Inc. Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies
US5629147A (en) * 1992-07-17 1997-05-13 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
JPH07509136A (en) * 1992-07-17 1995-10-12 バイシス，インク． Enrichment and identification of fetal cells in maternal blood for in situ (IN SITU) hybridization
WO1997005282A2 (en) * 1995-07-28 1997-02-13 Rijksuniversiteit Groningen Methods and materials for determining relative abundance of microorganisms in mixed populations
JP2001509662A (en) * 1996-04-19 2001-07-24 ファルマシア・アンド・アップジョン・カンパニー High-capacity in-situ mRNA hybridization method for quantification and discovery of disease-specific genes
DE69942309D1 (en) * 1998-05-18 2010-06-10 Igenex Inc IN SITU HYBRIDIZATION PROCEDURE FOR THE DISCOVERY OF TARGET NUCLEIC ACIDS
JP4557347B2 (en) * 2000-01-30 2010-10-06 株式会社カイノス Method for detecting a target nucleic acid having a specific base sequence or measuring the amount of the nucleic acid
US20040115692A1 (en) * 2000-04-03 2004-06-17 Cytyc Corporation Methods, compositions and apparatuses for detecting a target in a preservative solution
EP2563935B1 (en) 2010-04-30 2014-04-16 Exiqon A/S In situ hybridization method and buffer.
CN115768780A (en) * 2020-04-07 2023-03-07 领先细胞医疗诊断有限公司 Method for detecting target nucleic acid by in situ hybridization and kit therefor
CN120948678B (en) * 2025-10-17 2026-02-03 苏州药明康德新药开发有限公司 RNA drug stabilizer suitable for mass spectrum and fluorescence detection and application thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002173A1 (en) * 1988-08-31 1990-03-08 Research Development Foundation One-step in situ hybridization assay
JPH04505556A (en) * 1989-03-07 1992-10-01 シンジェン，インコーポレイテッド In situ hybridization in suspension for cell detection or isolation

Also Published As

Publication number Publication date
EP0357436A2 (en) 1990-03-07
EP0357436A3 (en) 1991-06-12
ATE153705T1 (en) 1997-06-15
EP0432221B1 (en) 1997-05-28
FI910957A0 (en) 1991-02-27
ZA896490B (en) 1990-06-27
EP0432221A4 (en) 1992-01-15
DK34191D0 (en) 1991-02-27
PT91589B (en) 1995-08-09
AU4343989A (en) 1990-03-23
JPH04502552A (en) 1992-05-14
DE68928080D1 (en) 1997-07-03
IL91405A (en) 1996-03-31
IL91405A0 (en) 1990-04-29
EP0432221A1 (en) 1991-06-19
DE68928080T2 (en) 1998-01-15
PT91589A (en) 1990-03-08
FI910957A7 (en) 1991-02-27
NZ230389A (en) 1991-11-26
WO1990002173A1 (en) 1990-03-08
AU636854B2 (en) 1993-05-13

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