DK3466978T3 - Fusionsprotein til forbedring af proteinekspression fra mål-mRNA - Google Patents

Fusionsprotein til forbedring af proteinekspression fra mål-mRNA Download PDF

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Publication number
DK3466978T3
DK3466978T3 DK17806675.9T DK17806675T DK3466978T3 DK 3466978 T3 DK3466978 T3 DK 3466978T3 DK 17806675 T DK17806675 T DK 17806675T DK 3466978 T3 DK3466978 T3 DK 3466978T3
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DK
Denmark
Prior art keywords
target mrna
enhance
fusion protein
protein
protein expression
Prior art date
Application number
DK17806675.9T
Other languages
English (en)
Inventor
Takahiro Nakamura
Yusuke Yagi
Original Assignee
Univ Kyushu Nat Univ Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kyushu Nat Univ Corp filed Critical Univ Kyushu Nat Univ Corp
Priority claimed from PCT/JP2017/020076 external-priority patent/WO2017209122A1/ja
Application granted granted Critical
Publication of DK3466978T3 publication Critical patent/DK3466978T3/da

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/06Fusion polypeptide containing a localisation/targetting motif containing a lysosomal/endosomal localisation signal
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK17806675.9T 2016-06-03 2017-05-30 Fusionsprotein til forbedring af proteinekspression fra mål-mRNA DK3466978T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662345252P 2016-06-03 2016-06-03
JP2016120524 2016-06-17
PCT/JP2017/020076 WO2017209122A1 (ja) 2016-06-03 2017-05-30 標的mRNAからのタンパク質発現量を向上させるための融合タンパク質

Publications (1)

Publication Number Publication Date
DK3466978T3 true DK3466978T3 (da) 2021-04-26

Family

ID=65463402

Family Applications (1)

Application Number Title Priority Date Filing Date
DK17806675.9T DK3466978T3 (da) 2016-06-03 2017-05-30 Fusionsprotein til forbedring af proteinekspression fra mål-mRNA

Country Status (8)

Country Link
US (1) US11136361B2 (da)
EP (1) EP3466978B1 (da)
JP (1) JP6928386B2 (da)
CN (1) CN109415446B (da)
BR (1) BR112018075010A2 (da)
DK (1) DK3466978T3 (da)
ES (1) ES2874230T3 (da)
PT (1) PT3466978T (da)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025106522A1 (en) * 2023-11-13 2025-05-22 Massachusetts Institute Of Technology Tuning chloroplast transgene expression from a synthetic operon

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005029067A2 (en) * 2003-09-24 2005-03-31 Oncotherapy Science, Inc. Method of diagnosing breast cancer
JP4665190B2 (ja) * 2005-02-10 2011-04-06 学校法人東京理科大学 遺伝子の転写調節方法
JP5083750B2 (ja) 2005-11-16 2012-11-28 独立行政法人産業技術総合研究所 細胞内発光イメージングのために最適化されたルシフェラーゼ遺伝子
CA2658683A1 (en) * 2006-07-21 2008-01-24 Centre National De La Recherche Scientifique (Cnrs) Positive cytomodulines to improve bioreactor productivity
WO2011111828A1 (ja) 2010-03-11 2011-09-15 ライオン株式会社 化粧料組成物
DK2784157T3 (da) 2011-10-21 2019-10-21 Univ Kyushu Nat Univ Corp Designfremgangsmåde til et rna-bindende protein under anvendelse af ppr-motiv og anvendelse deraf
AU2014258386B2 (en) * 2013-04-22 2018-10-25 Hiroshima University DNA binding protein using PPR motif, and use thereof
US10214766B2 (en) 2013-10-18 2019-02-26 National Institute Of Advanced Industrial Science And Technology Luminescent substrate for use in artificial bioluminescent enzyme
JP2015221026A (ja) * 2014-05-23 2015-12-10 公立大学法人名古屋市立大学 人工合成mRNAの翻訳効率化方法

Also Published As

Publication number Publication date
EP3466978A1 (en) 2019-04-10
US20190309029A1 (en) 2019-10-10
ES2874230T3 (es) 2021-11-04
BR112018075010A2 (pt) 2019-03-12
JP6928386B2 (ja) 2021-09-01
CN109415446A (zh) 2019-03-01
JPWO2017209122A1 (ja) 2019-04-04
US11136361B2 (en) 2021-10-05
PT3466978T (pt) 2021-05-07
CN109415446B (zh) 2022-07-19
EP3466978A4 (en) 2020-01-29
EP3466978B1 (en) 2021-04-07

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