DK3495498T3 - Genekspressionsanalyse i enkeltceller - Google Patents

Genekspressionsanalyse i enkeltceller Download PDF

Info

Publication number
DK3495498T3
DK3495498T3 DK18201978.6T DK18201978T DK3495498T3 DK 3495498 T3 DK3495498 T3 DK 3495498T3 DK 18201978 T DK18201978 T DK 18201978T DK 3495498 T3 DK3495498 T3 DK 3495498T3
Authority
DK
Denmark
Prior art keywords
gene expression
single cells
expression analysis
analysis
cells
Prior art date
Application number
DK18201978.6T
Other languages
English (en)
Inventor
Sten Linnarsson
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42936790&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK3495498(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Inc filed Critical Illumina Inc
Application granted granted Critical
Publication of DK3495498T3 publication Critical patent/DK3495498T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK18201978.6T 2009-03-30 2010-03-23 Genekspressionsanalyse i enkeltceller DK3495498T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16475909P 2009-03-30 2009-03-30
EP15190426.5A EP3002337B1 (en) 2009-03-30 2010-03-23 Gene expression analysis in single cells

Publications (1)

Publication Number Publication Date
DK3495498T3 true DK3495498T3 (da) 2022-01-17

Family

ID=42936790

Family Applications (3)

Application Number Title Priority Date Filing Date
DK15190426.5T DK3002337T3 (da) 2009-03-30 2010-03-23 Analyse af udtryk af gener i enkeltceller
DK18201978.6T DK3495498T3 (da) 2009-03-30 2010-03-23 Genekspressionsanalyse i enkeltceller
DK10762102.1T DK2414548T3 (da) 2009-03-30 2010-03-23 Genekspressionsanalyse i enkeltceller

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK15190426.5T DK3002337T3 (da) 2009-03-30 2010-03-23 Analyse af udtryk af gener i enkeltceller

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK10762102.1T DK2414548T3 (da) 2009-03-30 2010-03-23 Genekspressionsanalyse i enkeltceller

Country Status (5)

Country Link
US (1) US20120010091A1 (da)
EP (4) EP3002337B1 (da)
DK (3) DK3002337T3 (da)
ES (3) ES2555389T3 (da)
WO (1) WO2010117620A2 (da)

Families Citing this family (227)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060030036A1 (en) 2004-05-28 2006-02-09 Victor Joseph Chips for multiplex analyses
ES2990227T3 (es) 2008-01-17 2024-11-29 Sequenom Inc Composiciones y procedimientos de análisis de ácido nucleico de secuencia única
DK3002337T3 (da) 2009-03-30 2019-02-18 Illumina Inc Analyse af udtryk af gener i enkeltceller
KR101829182B1 (ko) 2009-04-02 2018-03-29 플루이다임 코포레이션 표적 핵산의 바코딩을 위한 멀티 프라이머 증폭 방법
EP2467479B1 (en) 2009-08-20 2016-01-06 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US9650629B2 (en) * 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
EP2625320B1 (en) 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
JP6069224B2 (ja) * 2011-01-31 2017-02-01 アプライズ バイオ, インコーポレイテッド 細胞において複数のエピトープを同定する方法
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
ES2625288T3 (es) 2011-04-15 2017-07-19 The Johns Hopkins University Sistema de secuenciación segura
PT3415619T (pt) * 2011-04-28 2021-03-22 Univ Leland Stanford Junior Identificação de polinucleótidos associados a uma amostra
SG10201605049QA (en) * 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
EP2739587B1 (en) 2011-08-01 2020-05-27 Denovo Sciences Cell capture system
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
WO2013096839A1 (en) 2011-12-22 2013-06-27 Somagenics, Inc. Methods of constructing small rna libraries and their use for expression profiling of target rnas
US10941396B2 (en) * 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
WO2013134261A1 (en) * 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
SG10201610861XA (en) * 2012-07-03 2017-02-27 Integrated Dna Tech Inc Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA3216609C (en) 2012-08-14 2024-05-14 10X Genomics, Inc. Microcapsule compositions and methods
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
EP2898096B1 (en) 2012-09-21 2024-02-14 The Broad Institute, Inc. Methods for labeling of rnas
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
EP2912197B1 (en) 2012-10-24 2019-08-07 Takara Bio USA, Inc. Template switch-based methods for producing a product nucleic acid
AU2013338393C1 (en) 2012-10-29 2024-07-25 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9752181B2 (en) 2013-01-26 2017-09-05 Denovo Sciences, Inc. System and method for capturing and analyzing cells
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
US9233305B2 (en) * 2013-02-13 2016-01-12 Unity Technologies Finland Oy System and method for managing game-playing experiences
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
JP6453300B2 (ja) * 2013-03-14 2019-01-16 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 細胞内分析のためのナノピペット装置および方法
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
US20160122753A1 (en) * 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
CN105579587A (zh) * 2013-08-23 2016-05-11 惠氏公司 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
EP4389888A3 (en) 2013-10-17 2024-09-04 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
EP3058100A4 (en) 2013-10-18 2017-04-19 California Institute of Technology Enhanced nucleic acid identification and detection
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015094861A1 (en) 2013-12-17 2015-06-25 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
SG11201609053YA (en) 2014-04-29 2016-11-29 Illumina Inc Multiplexed single cell gene expression analysis using template switch and tagmentation
US10975371B2 (en) * 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US20150360226A1 (en) 2014-06-12 2015-12-17 Wafergen, Inc. Single cell capture with polymer capture films
US9757707B2 (en) 2014-06-12 2017-09-12 Takara Bio Usa, Inc. Single cell capture with capture chips
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016904A (es) 2014-06-26 2017-03-27 10X Genomics Inc Analisis de secuencias de acidos nucleicos.
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
ES2895750T3 (es) * 2014-09-15 2022-02-22 Abvitro Llc Secuenciación de alto rendimiento de colecciones de nucleótidos
MX2017005267A (es) 2014-10-29 2017-07-26 10X Genomics Inc Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana.
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
HK1243466A1 (zh) 2014-11-05 2018-07-13 加州理工学院 生物体对药物的响应的微流体测量
CN112782140A (zh) 2014-12-03 2021-05-11 伊索普莱西斯公司 细胞分泌特征的分析和筛选
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
US10697010B2 (en) 2015-02-19 2020-06-30 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
JP6620160B2 (ja) 2015-02-20 2019-12-11 タカラ バイオ ユーエスエー, インコーポレイテッド 単一細胞の迅速かつ正確な分注、視覚化及び解析のための方法
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
BR112017018054A2 (pt) 2015-02-24 2018-07-24 10X Genomics Inc métodos para a cobertura de sequências de ácidos nucleicos direcionadas
JP2018509896A (ja) 2015-02-27 2018-04-12 フリューダイム・コーポレイション 高処理能力研究用の単一細胞核酸
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
WO2016168584A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
EP3341478B1 (en) * 2015-08-24 2019-11-06 Qiagen GmbH Method for generating a rna-sequencing library
EP4368715A3 (en) 2015-08-28 2024-07-24 Illumina, Inc. Nucleic acid sequence analysis from single cells
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
SG11201803646YA (en) 2015-11-04 2018-05-30 Atreca Inc Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017106777A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
US11014957B2 (en) * 2015-12-21 2021-05-25 Realseq Biosciences, Inc. Methods of library construction for polynucleotide sequencing
JP6735348B2 (ja) 2016-02-11 2020-08-05 10エックス ジェノミクス, インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
US10633648B2 (en) 2016-02-12 2020-04-28 University Of Washington Combinatorial photo-controlled spatial sequencing and labeling
ES2956757T3 (es) 2016-05-02 2023-12-27 Becton Dickinson Co Codificación con códigos de barras moleculares precisa
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (en) 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CA3020629A1 (en) 2016-07-21 2018-01-25 Takara Bio Usa, Inc. Multi-z imaging and dispensing with multi-well devices
EP3510398B1 (en) 2016-09-12 2026-02-25 Isoplexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
US11168347B2 (en) 2016-09-23 2021-11-09 California Institute Of Technology Digital quantification of DNA replication and/or chromosome segregation based determination of antimicrobial susceptibility
CA3034924C (en) 2016-09-26 2025-11-18 Becton, Dickinson And Company MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES
EP3518974A4 (en) * 2016-09-29 2020-05-27 Myriad Women's Health, Inc. NON-INVASIVE PRENATAL SCREENING USING DYNAMIC ITERATIVE DEEP OPTIMIZATION
CN117056774B (zh) 2016-11-08 2026-01-27 贝克顿迪金森公司 用于细胞标记分类的方法
CN109952612B (zh) 2016-11-08 2023-12-01 贝克顿迪金森公司 用于表达谱分类的方法
EP4036578A1 (en) 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法,以及其制造方法
EP4357455A3 (en) * 2016-12-12 2024-07-24 Grail, LLC Methods for tagging and amplifying rna template molecules for preparing sequencing libraries
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN110573253B (zh) 2017-01-13 2021-11-02 赛卢拉研究公司 流体通道的亲水涂层
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
CN110214186B (zh) 2017-01-30 2023-11-24 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
CN107083423B (zh) * 2017-03-27 2022-01-28 北京极客基因科技有限公司 一种药物靶点预测和药物全方面评价方法
US11028435B2 (en) 2017-05-01 2021-06-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
ES2989056T3 (es) * 2017-05-08 2024-11-25 Illumina Inc Método de secuenciación usando adaptadores cortos universales para la indexación de muestras de polinucleótidos
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
ES2926722T3 (es) 2017-05-26 2022-10-27 Abvitro Llc Secuenciación de alto rendimiento de biblioteca de polinucleótidos y análisis de transcriptoma
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
US10636512B2 (en) 2017-07-14 2020-04-28 Cofactor Genomics, Inc. Immuno-oncology applications using next generation sequencing
IL319255A (en) 2017-08-07 2025-04-01 Univ Johns Hopkins Methods and materials for cancer assessment and treatment
WO2019046307A1 (en) 2017-08-29 2019-03-07 Celsee Diagnostics, Inc. SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019075264A1 (en) 2017-10-11 2019-04-18 California Institute Of Technology ANTIBIOSENSITIVITY OF MICROORGANISMS AND COMPOSITIONS, METHODS AND ASSOCIATED SYSTEMS
US12448655B2 (en) 2017-10-11 2025-10-21 California Institute Of Technology Antibiotic susceptibility of microorganisms and related methods and systems
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN114525273B (zh) 2017-10-27 2025-01-28 10X基因组学有限公司 用于样品制备和分析的方法和系统
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN108048915A (zh) * 2017-12-01 2018-05-18 北京科迅生物技术有限公司 用于ctDNA文库构建的接头混合物、包括其的试剂盒及应用
WO2019113457A1 (en) 2017-12-07 2019-06-13 Massachusetts Institute Of Technology Single cell analyses
CN118818037A (zh) 2017-12-12 2024-10-22 10X基因组学有限公司 用于单细胞处理的系统和方法
CN111492068B (zh) 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
JP2021509024A (ja) 2018-01-02 2021-03-18 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン 多数の液滴の捕捉
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3830289B1 (en) 2018-08-03 2026-02-11 10X Genomics, Inc. Methods to minimize barcode exchange
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
ES2992135T3 (es) * 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
JP7618548B2 (ja) * 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
US12545950B2 (en) * 2018-12-07 2026-02-10 Qiagen Sciences, Llc Methods for preparing CDNA samples for RNA sequencing, and CDNA samples and uses thereof
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
WO2020142768A1 (en) 2019-01-04 2020-07-09 Northwestern University Storing temporal data into dna
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113767178A (zh) 2019-03-11 2021-12-07 10X基因组学有限公司 用于处理光学标签化珠粒的系统和方法
CN113811619A (zh) 2019-03-27 2021-12-17 10X基因组学有限公司 用于处理来自细胞的rna的系统和方法
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
EP3966307B1 (en) 2019-05-07 2024-11-20 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
SG11202112898WA (en) 2019-06-14 2021-12-30 Bio Rad Laboratories System and method for automated single cell processing and analyses
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
US11827936B2 (en) 2020-01-13 2023-11-28 Fluent Biosciences Inc. Methods and systems for single cell gene profiling
WO2021146184A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Single cell sequencing
WO2021146187A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Emulsion based drug screening
EP4090764A1 (en) 2020-01-13 2022-11-23 Becton, Dickinson and Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US20210238661A1 (en) * 2020-01-31 2021-08-05 Becton, Dickinson And Company Mesophilic dna polymerase extension blockers
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
JP2023513606A (ja) 2020-02-14 2023-03-31 ザ・ジョンズ・ホプキンス・ユニバーシティー 核酸を評価するための方法および材料
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
EP4121016A4 (en) 2020-03-16 2024-09-11 Fluent Biosciences Inc. MULTI-OMICS ANALYSIS IN MONODISPERSED DROPLETS
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4157300A4 (en) * 2020-05-29 2024-07-17 BioHSV Holdings, Inc. MASS NUCLEIC ACID TEST
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12241059B2 (en) 2020-07-15 2025-03-04 Illumina, Inc. Tiered ligation oligos
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
JP7839145B2 (ja) 2020-08-06 2026-04-01 イルミナ インコーポレイテッド ビーズ連結トランスポソームを使用するrna及びdna配列決定ライブラリの調製
WO2022069039A1 (en) * 2020-09-30 2022-04-07 Ecole Polytechnique Federale De Lausanne (Epfl) METHOD OF PREPARATION OF cDNA LIBRARY USEFUL FOR EFFICIENT mRNA SEQUENCING AND USES THEREOF
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4263859A1 (en) 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
CN120158497A (zh) 2021-02-23 2025-06-17 10X基因组学有限公司 基于探针的核酸和蛋白质分析
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
WO2023107539A1 (en) 2021-12-10 2023-06-15 Bio-Rad Laboratories, Inc. Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles
US12534721B2 (en) 2022-10-06 2026-01-27 Illumina, Inc. Quantitative detection and analysis of moleculesip
CN116200474A (zh) * 2022-12-30 2023-06-02 阅尔基因技术(苏州)有限公司 一种分析颗粒细胞基因表达的方法
EP4435114A1 (en) 2023-03-24 2024-09-25 Miltenyi Biotec B.V. & Co. KG Syntheis of highly multiplexed oligo-barcoded fluorescent beads for single cell seq applications

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5681702A (en) 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6480791B1 (en) * 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US6197554B1 (en) * 1998-11-20 2001-03-06 Shi-Lung Lin Method for generating full-length cDNA library from single cells
WO2002008461A2 (en) * 2000-07-21 2002-01-31 Global Genomics Ab A METHOD AND AN ALGORITHM FOR mRNA EXPRESSION ANALYSIS
US6706476B1 (en) 2000-08-22 2004-03-16 Azign Bioscience A/S Process for amplifying and labeling single stranded cDNA by 5′ ligated adaptor mediated amplification
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
AU2003299541A1 (en) 2002-10-02 2004-05-25 California Institute Of Technology Microfluidic nucleic acid analysis
GB0319332D0 (en) 2003-08-16 2003-09-17 Astrazeneca Ab Amplification
DE602004026077D1 (de) 2003-09-10 2010-04-29 Althea Technologies Inc Erstellung von expressionsprofilen unter verwendung von mikroarrays
EP1776458A2 (en) 2004-07-30 2007-04-25 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
JP2009508495A (ja) * 2005-09-16 2009-03-05 454 ライフ サイエンシーズ コーポレイション cDNAライブラリー調製
JP5073967B2 (ja) 2006-05-30 2012-11-14 株式会社日立製作所 単一細胞の遺伝子発現定量方法
WO2007147079A2 (en) * 2006-06-14 2007-12-21 Living Microsystems, Inc. Rare cell analysis using sample splitting and dna tags
US20080108804A1 (en) 2006-11-02 2008-05-08 Kabushiki Kaisha Dnaform Method for modifying RNAS and preparing DNAS from RNAS
EP2164985A4 (en) 2007-06-01 2014-05-14 454 Life Sciences Corp SYSTEM AND METHOD FOR IDENTIFYING INDIVIDUAL SAMPLES FROM A MULTIPLEX MIXTURE
US8039214B2 (en) 2007-06-29 2011-10-18 Cellscript, Inc. Synthesis of tagged nucleic acids
US20100035249A1 (en) 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
DK3002337T3 (da) 2009-03-30 2019-02-18 Illumina Inc Analyse af udtryk af gener i enkeltceller

Also Published As

Publication number Publication date
WO2010117620A3 (en) 2011-02-17
EP3495498B1 (en) 2021-10-27
EP2414548B1 (en) 2015-10-21
DK2414548T3 (da) 2015-12-21
ES2903425T3 (es) 2022-04-01
EP3495498A1 (en) 2019-06-12
HK1221266A1 (en) 2017-05-26
WO2010117620A2 (en) 2010-10-14
ES2706227T3 (es) 2019-03-27
DK3002337T3 (da) 2019-02-18
US20120010091A1 (en) 2012-01-12
EP2414548A2 (en) 2012-02-08
EP3998346A1 (en) 2022-05-18
EP2414548A4 (en) 2012-10-10
EP3002337B1 (en) 2018-10-24
ES2555389T3 (es) 2015-12-30
EP3002337A1 (en) 2016-04-06

Similar Documents

Publication Publication Date Title
DK3495498T3 (da) Genekspressionsanalyse i enkeltceller
IL254144A0 (en) Methods for reprogramming cells and uses thereof
BRPI1010035A2 (pt) Células deficientes em fucosilação
BRPI0920956A2 (pt) Cultura de células-tronco pluripotentes em microveículos
DK2344682T3 (da) Elektrode for elektrolysecelle
PL2634242T3 (pl) Ulepszony sposób hodowli komórek
BR112012002974A2 (pt) Produção de proteínas em meio de cultura de célula livre de glutamina
DE102008055028A8 (de) Solarzelle
DK3442233T3 (da) Nedarvning i samplearraymultitræsunderinddeling
PL2396036T3 (pl) Immunokoniugaty z połączeniem rozszczepialnym wewnątrzkomórkowo
DK2574677T3 (da) Fremgangsmåder til celledyrkning
FI20106121A7 (fi) Soluviljelymateriaali
EP2391711A4 (en) NEW CELL LINES AND METHOD
BR112012014158A2 (pt) "célula eletroquímica"
DK3192865T3 (da) Enkelt pluripotent stamcelle-kultur
EP2128242A4 (en) CELL CULTURE MODULE
DK2274430T3 (da) Nerveregenererende celler med ændringer i dna-methylering
EP2467882A4 (en) SOLAR CELL
BRPI0907886A2 (pt) Célula de lítio
EP2362470A4 (en) FUEL CELL
BRPI0923585A2 (pt) célula de pesagem
DE102008055036A8 (de) Solarzelle
EP2386124A4 (en) SOLAR CELL
EP2395565A4 (en) SOLAR CELL
EP2429022A4 (en) FUEL CELL SYSTEM