DK3498865T3 - Påvisning og kvantificering af enkeltmolekyler ved hjælp af DNA-nanoteknologi i microwells - Google Patents

Påvisning og kvantificering af enkeltmolekyler ved hjælp af DNA-nanoteknologi i microwells Download PDF

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DK3498865T3
DK3498865T3 DK17207476.7T DK17207476T DK3498865T3 DK 3498865 T3 DK3498865 T3 DK 3498865T3 DK 17207476 T DK17207476 T DK 17207476T DK 3498865 T3 DK3498865 T3 DK 3498865T3
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microwells
quantification
detection
single molecules
dna nanotechnology
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DK17207476.7T
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Johannes Benedikt Wöhrstein
Heinrich Ernst Paul Grabmayr
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Univ Muenchen Ludwig Maximilians
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Rigid containers without fluid transport within
    • B01L3/5085Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B01L3/50853Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates with covers or lids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/157Nanotubes or nanorods
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10Detection mode being characterised by the assay principle
    • C12Q2565/102Multiple non-interacting labels

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK17207476.7T 2017-12-14 2017-12-14 Påvisning og kvantificering af enkeltmolekyler ved hjælp af DNA-nanoteknologi i microwells DK3498865T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP17207476.7A EP3498865B1 (de) 2017-12-14 2017-12-14 Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie in mikro-wells

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DK3498865T3 true DK3498865T3 (da) 2020-12-21

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DK17207476.7T DK3498865T3 (da) 2017-12-14 2017-12-14 Påvisning og kvantificering af enkeltmolekyler ved hjælp af DNA-nanoteknologi i microwells

Country Status (11)

Country Link
US (1) US20200354777A1 (da)
EP (2) EP3805405A1 (da)
JP (1) JP2021509013A (da)
KR (1) KR20200098624A (da)
CN (1) CN111479930A (da)
AU (1) AU2018382598A1 (da)
CA (1) CA3087850A1 (da)
DK (1) DK3498865T3 (da)
IL (1) IL275101A (da)
RU (1) RU2020123137A (da)
WO (1) WO2019115801A1 (da)

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EP3884048A4 (en) 2018-11-20 2022-08-17 Nautilus Biotechnology, Inc. DESIGN AND SELECTION OF AFFINITY REAGENTS
US11976269B2 (en) * 2019-03-18 2024-05-07 Cellular Research, Inc. Precise delivery of components into fluids
CA3135206A1 (en) 2019-04-29 2020-11-05 Pierre Indermuhle Methods and systems for integrated on-chip single-molecule detection
CN112301162B (zh) * 2020-10-22 2023-09-22 清华-伯克利深圳学院筹备办公室 同时检测dna病毒和rna病毒的病毒核酸检测方法
CN113049552B (zh) * 2021-03-07 2022-08-05 天津大学 基于外泌体检测和单分子荧光漂白技术的muc1蛋白定量检测方法
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
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US20230227892A1 (en) * 2022-01-14 2023-07-20 Mbiomics Gmbh Method of Determining a Quantitative Fingerprint of a Subset of Bacteria in a Person's Gastrointestinal Microbiome
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DE102022131448A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
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DE102022131445A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131450A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131451A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge
CN116832178A (zh) * 2023-02-23 2023-10-03 上海交通大学医学院附属第九人民医院 向听觉细胞递送dna的方法、载体及制取方法和应用
DE102023122213A1 (de) * 2023-08-18 2025-02-20 Carl Zeiss Microscopy Gmbh Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen

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Also Published As

Publication number Publication date
EP3805405A1 (de) 2021-04-14
IL275101A (en) 2020-07-30
CN111479930A (zh) 2020-07-31
EP3498865A1 (de) 2019-06-19
US20200354777A1 (en) 2020-11-12
AU2018382598A1 (en) 2020-06-18
JP2021509013A (ja) 2021-03-18
WO2019115801A1 (de) 2019-06-20
KR20200098624A (ko) 2020-08-20
RU2020123137A (ru) 2022-01-14
CA3087850A1 (en) 2019-06-20
EP3498865B1 (de) 2020-10-07

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