DK3498865T3 - Påvisning og kvantificering af enkeltmolekyler ved hjælp af DNA-nanoteknologi i microwells - Google Patents
Påvisning og kvantificering af enkeltmolekyler ved hjælp af DNA-nanoteknologi i microwells Download PDFInfo
- Publication number
- DK3498865T3 DK3498865T3 DK17207476.7T DK17207476T DK3498865T3 DK 3498865 T3 DK3498865 T3 DK 3498865T3 DK 17207476 T DK17207476 T DK 17207476T DK 3498865 T3 DK3498865 T3 DK 3498865T3
- Authority
- DK
- Denmark
- Prior art keywords
- microwells
- quantification
- detection
- single molecules
- dna nanotechnology
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Rigid containers without fluid transport within
- B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
- B01L3/50853—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates with covers or lids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/157—Nanotubes or nanorods
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/102—Multiple non-interacting labels
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17207476.7A EP3498865B1 (de) | 2017-12-14 | 2017-12-14 | Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie in mikro-wells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3498865T3 true DK3498865T3 (da) | 2020-12-21 |
Family
ID=60937499
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17207476.7T DK3498865T3 (da) | 2017-12-14 | 2017-12-14 | Påvisning og kvantificering af enkeltmolekyler ved hjælp af DNA-nanoteknologi i microwells |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20200354777A1 (da) |
| EP (2) | EP3805405A1 (da) |
| JP (1) | JP2021509013A (da) |
| KR (1) | KR20200098624A (da) |
| CN (1) | CN111479930A (da) |
| AU (1) | AU2018382598A1 (da) |
| CA (1) | CA3087850A1 (da) |
| DK (1) | DK3498865T3 (da) |
| IL (1) | IL275101A (da) |
| RU (1) | RU2020123137A (da) |
| WO (1) | WO2019115801A1 (da) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111566261B (zh) | 2017-08-18 | 2025-03-25 | 诺迪勒思附属公司 | 选择结合试剂的方法 |
| EP3884048A4 (en) | 2018-11-20 | 2022-08-17 | Nautilus Biotechnology, Inc. | DESIGN AND SELECTION OF AFFINITY REAGENTS |
| US11976269B2 (en) * | 2019-03-18 | 2024-05-07 | Cellular Research, Inc. | Precise delivery of components into fluids |
| CA3135206A1 (en) | 2019-04-29 | 2020-11-05 | Pierre Indermuhle | Methods and systems for integrated on-chip single-molecule detection |
| CN112301162B (zh) * | 2020-10-22 | 2023-09-22 | 清华-伯克利深圳学院筹备办公室 | 同时检测dna病毒和rna病毒的病毒核酸检测方法 |
| CN113049552B (zh) * | 2021-03-07 | 2022-08-05 | 天津大学 | 基于外泌体检测和单分子荧光漂白技术的muc1蛋白定量检测方法 |
| IL305336B2 (en) | 2021-03-11 | 2025-09-01 | Nautilus Subsidiary Inc | Systems and methods for preserving biomolecules |
| KR102462745B1 (ko) * | 2021-04-29 | 2022-11-04 | 재단법인 아산사회복지재단 | 형광 상관 분광법을 이용한 세포외소포체에 표지된 형광 염료의 정량 분석 방법 및 이의 용도 |
| CA3222949A1 (en) * | 2021-06-14 | 2022-12-22 | Mbiomics Gmbh | System and method for performing a microbiome-analysis |
| KR102712830B1 (ko) * | 2021-11-30 | 2024-10-02 | 주식회사 바이오티엔에스 | 표적 유전자의 정량 분석 방법 및 이를 이용한 디바이스 |
| US20230227892A1 (en) * | 2022-01-14 | 2023-07-20 | Mbiomics Gmbh | Method of Determining a Quantitative Fingerprint of a Subset of Bacteria in a Person's Gastrointestinal Microbiome |
| DE102022131446A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131444A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren zum Identifizieren von Analyten in einer Bildfolge |
| DE102022131447A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131448A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131449A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131445A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131450A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131451A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge |
| CN116832178A (zh) * | 2023-02-23 | 2023-10-03 | 上海交通大学医学院附属第九人民医院 | 向听觉细胞递送dna的方法、载体及制取方法和应用 |
| DE102023122213A1 (de) * | 2023-08-18 | 2025-02-20 | Carl Zeiss Microscopy Gmbh | Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005253412A (ja) * | 2004-03-15 | 2005-09-22 | Masayasu Suzuki | マイクロウェルアレイチップ、その製造方法及び被検体の活性検定方法 |
| GB201103665D0 (en) * | 2011-03-04 | 2011-04-13 | Univ Cardiff | Correlative microscopy |
| US9796748B2 (en) * | 2011-05-02 | 2017-10-24 | President And Fellows Of Harvard College | Spatial sequestration of dynamic nucleic acid circuits |
| US20160145679A1 (en) * | 2013-03-01 | 2016-05-26 | Arizona Board Of Regents On Behalf Of Arizona State University | Oligonucleotide functionalized quantum dots |
| WO2014145620A2 (en) * | 2013-03-15 | 2014-09-18 | The Broad Institute, Inc. | Novel hybridization probes and uses thereof |
| AU2014296253A1 (en) * | 2013-07-30 | 2016-02-04 | President And Fellows Of Harvard College | Quantitative DNA-based imaging and super-resolution imaging |
| ES2711168T3 (es) * | 2013-08-28 | 2019-04-30 | Becton Dickinson Co | Análisis masivo en paralelo de células individuales |
| WO2016140726A2 (en) | 2014-12-16 | 2016-09-09 | President And Fellows Of Harvard College | Triggered assembly of metafluorophores |
| EP3248018B1 (en) * | 2015-01-22 | 2020-01-08 | Becton, Dickinson and Company | Devices and systems for molecular barcoding of nucleic acid targets in single cells |
| CN105032512B (zh) * | 2015-08-25 | 2017-01-04 | 辽宁中医药大学 | 用于药物配伍筛选的集成化微流控芯片、制备方法及应用 |
| EP3353323B1 (en) * | 2015-09-22 | 2022-03-23 | Life Technologies Corporation | Systems and methods for analysis of nucleic acids |
| JP6763127B2 (ja) * | 2015-10-07 | 2020-09-30 | 凸版印刷株式会社 | マイクロウェルアレイ、マイクロ流体デバイス、マイクロウェルアレイのウェル内に水性液体を封入する方法及びマイクロウェルアレイの製造方法 |
| EP3472351B1 (de) * | 2016-06-15 | 2020-10-28 | Ludwig-Maximilians-Universität München | Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie |
-
2017
- 2017-12-14 DK DK17207476.7T patent/DK3498865T3/da active
- 2017-12-14 EP EP20200076.6A patent/EP3805405A1/de not_active Withdrawn
- 2017-12-14 EP EP17207476.7A patent/EP3498865B1/de active Active
-
2018
- 2018-12-14 KR KR1020207020165A patent/KR20200098624A/ko not_active Withdrawn
- 2018-12-14 WO PCT/EP2018/085046 patent/WO2019115801A1/de not_active Ceased
- 2018-12-14 RU RU2020123137A patent/RU2020123137A/ru unknown
- 2018-12-14 CN CN201880080718.2A patent/CN111479930A/zh active Pending
- 2018-12-14 AU AU2018382598A patent/AU2018382598A1/en not_active Abandoned
- 2018-12-14 US US16/771,612 patent/US20200354777A1/en not_active Abandoned
- 2018-12-14 CA CA3087850A patent/CA3087850A1/en active Pending
- 2018-12-14 JP JP2020532783A patent/JP2021509013A/ja active Pending
-
2020
- 2020-06-03 IL IL275101A patent/IL275101A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3805405A1 (de) | 2021-04-14 |
| IL275101A (en) | 2020-07-30 |
| CN111479930A (zh) | 2020-07-31 |
| EP3498865A1 (de) | 2019-06-19 |
| US20200354777A1 (en) | 2020-11-12 |
| AU2018382598A1 (en) | 2020-06-18 |
| JP2021509013A (ja) | 2021-03-18 |
| WO2019115801A1 (de) | 2019-06-20 |
| KR20200098624A (ko) | 2020-08-20 |
| RU2020123137A (ru) | 2022-01-14 |
| CA3087850A1 (en) | 2019-06-20 |
| EP3498865B1 (de) | 2020-10-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK3498865T3 (da) | Påvisning og kvantificering af enkeltmolekyler ved hjælp af DNA-nanoteknologi i microwells | |
| IL283416A (en) | Genomic infrastructure for on-site or cloud-based dna and rna processing and analysis | |
| IL261942A (en) | Bcma binding molecules and methods of use thereof | |
| IL272463A (en) | Nucleic acid molecules and uses thereof | |
| DK3981792T3 (da) | PD-1-bindende molekyler og fremgangsmåder til anvendelse deraf | |
| DK3380522T5 (da) | Antistofmolekyler til april og anvendelser deraf | |
| DK3673081T3 (da) | Nøjagtig og massivt parallel kvantificering af nukleinsyre | |
| EP3559259A4 (en) | DECONVOLUTION AND DETECTION OF RARE DNA IN PLASMA | |
| DK3516055T3 (da) | Skalerbar bioteknologisk fremstilling af enkeltstrengede dna-molekyler med defineret sekvens og længde | |
| DK3701959T3 (da) | RNA-Replikon til alsidig og effektiv genekspression | |
| DK3116909T3 (da) | Antistofmolekyler til lag-3 og anvendelser deraf | |
| DK3882357T3 (da) | Fremgangsmåder og sammensætninger til analyse af nukleinsyrer | |
| DK3289362T3 (da) | Detektering og sortering af mikrofluid-smådråber | |
| DK3114225T3 (da) | Sammensætning og fremgangsmåde til stabilisering af nukleinsyrer i biologiske prøver | |
| IL259036B (en) | Binding molecules specific for asct2 and uses thereof | |
| DK3411151T3 (da) | Mikrofluidt assaysystem og fremgangsmåde til at udføre et assay | |
| IL261653A (en) | Ilt7 binding molecules and methods of using the same | |
| DK3433273T3 (da) | Binding af molekyler til cd38 og pd-l1 | |
| DK3254088T3 (da) | Fotoakustisk sensorsystem og metodologi til detektering af målmolekyler | |
| DK3268315T3 (da) | System og fremgangsmåde til behandling af fluider ved hjælp af sonoelektrokemi | |
| DK3562946T3 (da) | Oncolytiske virusser og terapeutiske molekyler | |
| DK3298162T3 (da) | Detektering af målnukleinsyre og varianter | |
| PL3443115T3 (pl) | Sposób i zestaw do generowania bibliotek DNA do masywnego równoległego sekwencjonowania | |
| DK3417058T3 (da) | Sortase-modificerede molekyler og anvendelser deraf | |
| EP3094767A4 (en) | Use of nucleic acid agents for ultra-sensitive digital detection and quantification of target molecules |