DK3674404T3 - Målsekvensspecifik ændringsteknologi under anvendelse af nukleotidmålgenkendelse - Google Patents

Målsekvensspecifik ændringsteknologi under anvendelse af nukleotidmålgenkendelse

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Publication number
DK3674404T3
DK3674404T3 DK18847639.4T DK18847639T DK3674404T3 DK 3674404 T3 DK3674404 T3 DK 3674404T3 DK 18847639 T DK18847639 T DK 18847639T DK 3674404 T3 DK3674404 T3 DK 3674404T3
Authority
DK
Denmark
Prior art keywords
specific modification
modification technology
nucleotide
target
target sequence
Prior art date
Application number
DK18847639.4T
Other languages
English (en)
Inventor
Keishi Osakabe
Yuriko Osakabe
Original Assignee
Univ Tokushima
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Tokushima filed Critical Univ Tokushima
Application granted granted Critical
Publication of DK3674404T3 publication Critical patent/DK3674404T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111General methods applicable to biologically active non-coding nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Discharge Of Articles From Conveyors (AREA)
  • Control Of Conveyors (AREA)
  • Branching, Merging, And Special Transfer Between Conveyors (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK18847639.4T 2017-08-21 2018-08-20 Målsekvensspecifik ændringsteknologi under anvendelse af nukleotidmålgenkendelse DK3674404T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2017158876 2017-08-21
JP2017236518 2017-12-08
PCT/JP2018/030607 WO2019039417A1 (ja) 2017-08-21 2018-08-20 ヌクレオチド標的認識を利用した標的配列特異的改変技術

Publications (1)

Publication Number Publication Date
DK3674404T3 true DK3674404T3 (da) 2025-12-15

Family

ID=65438898

Family Applications (1)

Application Number Title Priority Date Filing Date
DK18847639.4T DK3674404T3 (da) 2017-08-21 2018-08-20 Målsekvensspecifik ændringsteknologi under anvendelse af nukleotidmålgenkendelse

Country Status (12)

Country Link
US (3) US12012596B2 (da)
EP (1) EP3674404B1 (da)
JP (2) JP7017259B2 (da)
KR (1) KR102626503B1 (da)
CN (1) CN111247247A (da)
AU (1) AU2018321021B2 (da)
BR (1) BR112020003439A2 (da)
DK (1) DK3674404T3 (da)
IL (1) IL272688B2 (da)
MX (1) MX2020001998A (da)
SG (1) SG11202001471SA (da)
WO (1) WO2019039417A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3636753T5 (da) * 2017-06-08 2024-08-26 Univ Osaka Fremgangsmåde til fremstilling af DNA-redigeret eukaryotisk celle
WO2020122104A1 (ja) * 2018-12-11 2020-06-18 国立大学法人京都大学 ゲノムdnaに欠失を誘導する方法
WO2020184723A1 (ja) * 2019-03-14 2020-09-17 国立大学法人徳島大学 Crisprタイプi-dシステムを利用した標的配列改変技術
CN113646429B (zh) 2019-04-05 2025-07-11 国立大学法人大阪大学 敲入细胞的制作方法
KR102550308B1 (ko) * 2020-09-29 2023-06-30 경상국립대학교산학협력단 SlHKT1;2 유전자 교정에 의해 내염성이 증가된 유전체 교정 토마토 식물체의 제조 방법 및 상기 방법에 의해 제조된 내염성이 증가된 유전체 교정 토마토 식물체
JP7454881B2 (ja) * 2020-10-08 2024-03-25 国立大学法人徳島大学 Crisprタイプi-dシステムを利用した標的ヌクレオチド配列改変技術
CN112921038B (zh) * 2021-02-23 2022-08-02 安徽农业大学 同源重组机制介导的精准序列替换基因编辑方法及其元件结构
JPWO2024204287A1 (da) 2023-03-27 2024-10-03

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100102580A1 (en) 2008-10-23 2010-04-29 Brooks Ryan J Energy absorber with differentiating angled walls
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
JP2017512481A (ja) * 2014-04-08 2017-05-25 ノースカロライナ ステート ユニバーシティーNorth Carolina State University Crispr関連遺伝子を用いた、rna依存性の転写抑制のための方法および組成物
HRP20200529T1 (hr) * 2014-06-06 2020-09-04 Regeneron Pharmaceuticals, Inc. Postupci i pripravci za modificiranje ciljnog lokusa
ES2781323T3 (es) * 2014-06-23 2020-09-01 Regeneron Pharma Montaje de ADN mediado por nucleasas
EP3348636B1 (en) 2015-09-09 2021-12-01 National University Corporation Kobe University Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method
EP4089175A1 (en) * 2015-10-13 2022-11-16 Duke University Genome engineering with type i crispr systems in eukaryotic cells
DK3636753T5 (da) * 2017-06-08 2024-08-26 Univ Osaka Fremgangsmåde til fremstilling af DNA-redigeret eukaryotisk celle

Also Published As

Publication number Publication date
AU2018321021A1 (en) 2020-03-26
SG11202001471SA (en) 2020-03-30
JP2022009293A (ja) 2022-01-14
AU2018321021B2 (en) 2022-10-06
WO2019039417A1 (ja) 2019-02-28
JP7017259B2 (ja) 2022-02-08
KR20200039775A (ko) 2020-04-16
US12012596B2 (en) 2024-06-18
EP3674404A1 (en) 2020-07-01
KR102626503B1 (ko) 2024-01-17
NZ762361A (en) 2024-03-22
IL272688B2 (en) 2025-03-01
US20240327828A1 (en) 2024-10-03
MX2020001998A (es) 2020-10-05
JP7054283B2 (ja) 2022-04-13
IL272688B1 (en) 2024-11-01
US20210363520A1 (en) 2021-11-25
EP3674404B1 (en) 2025-10-15
CA3073372A1 (en) 2019-02-28
US20240392288A1 (en) 2024-11-28
EP3674404A4 (en) 2021-05-26
CN111247247A (zh) 2020-06-05
JPWO2019039417A1 (ja) 2020-11-26
BR112020003439A2 (pt) 2020-08-25
IL272688A (en) 2020-04-30

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