DK3697804T3 - REKOMBINANT EKSPRESSION AF PCV2b-ORF2-PROTEIN I INSEKTCELLER - Google Patents

REKOMBINANT EKSPRESSION AF PCV2b-ORF2-PROTEIN I INSEKTCELLER Download PDF

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Publication number
DK3697804T3
DK3697804T3 DK18783522.8T DK18783522T DK3697804T3 DK 3697804 T3 DK3697804 T3 DK 3697804T3 DK 18783522 T DK18783522 T DK 18783522T DK 3697804 T3 DK3697804 T3 DK 3697804T3
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Prior art keywords
pcv2b
recombinant expression
insect cells
orf2 protein
orf2
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DK18783522.8T
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English (en)
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DK3697804T5 (da
Inventor
Paulus Jacobus Antonius Sondermeijer
Lisette Sanders
Der Heijden - Liefkens Karin Huberdina A Van
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Intervet Int Bv
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/39Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/20Antivirals for DNA viruses
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0601Invertebrate cells or tissues, e.g. insect cells; Culture media therefor
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    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/51Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/515Animal cells
    • A61K2039/5156Animal cells expressing foreign proteins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/51Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525Virus
    • A61K2039/5258Virus-like particles
    • AHUMAN NECESSITIES
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/545Medicinal preparations containing antigens or antibodies characterised by the dose, timing or administration schedule
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/55Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
    • A61K2039/552Veterinary vaccine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/555Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55505Inorganic adjuvants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/555Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511Organic adjuvants
    • A61K2039/55566Emulsions, e.g. Freund's adjuvant, MF59
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    • C12N2710/00011Details
    • C12N2710/14011Baculoviridae
    • C12N2710/14041Use of virus, viral particle or viral elements as a vector
    • C12N2710/14043Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vectore
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2750/00011Details
    • C12N2750/10011Circoviridae
    • C12N2750/10022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
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    • C12N2750/10011Circoviridae
    • C12N2750/10023Virus like particles [VLP]
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    • C12N2750/00011Details
    • C12N2750/10011Circoviridae
    • C12N2750/10034Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2750/10051Methods of production or purification of viral material
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    • C12N2799/00Uses of viruses
    • C12N2799/02Uses of viruses as vector
    • C12N2799/021Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Communicable Diseases (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK18783522.8T 2017-10-17 2018-10-16 REKOMBINANT EKSPRESSION AF PCV2b-ORF2-PROTEIN I INSEKTCELLER DK3697804T5 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17196768 2017-10-17
PCT/EP2018/078189 WO2019076864A1 (en) 2017-10-17 2018-10-16 RECOMBINANT EXPRESSION OF PCV2B ORF2 PROTEIN IN INSECT CELLS

Publications (2)

Publication Number Publication Date
DK3697804T3 true DK3697804T3 (da) 2023-11-13
DK3697804T5 DK3697804T5 (da) 2024-08-26

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ID=60186018

Family Applications (1)

Application Number Title Priority Date Filing Date
DK18783522.8T DK3697804T5 (da) 2017-10-17 2018-10-16 REKOMBINANT EKSPRESSION AF PCV2b-ORF2-PROTEIN I INSEKTCELLER

Country Status (13)

Country Link
US (1) US11279952B2 (da)
EP (1) EP3697804B1 (da)
JP (2) JP7062760B2 (da)
KR (1) KR102480771B1 (da)
CN (1) CN111212847B (da)
BR (1) BR112020007498A2 (da)
CA (1) CA3078281C (da)
DK (1) DK3697804T5 (da)
ES (1) ES2962449T3 (da)
HU (1) HUE064720T2 (da)
MX (1) MX385295B (da)
PL (1) PL3697804T3 (da)
WO (1) WO2019076864A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111212847B (zh) * 2017-10-17 2024-01-30 英特维特国际股份有限公司 PCV2b ORF2蛋白在昆虫细胞中的重组表达
CN115960259B (zh) * 2022-10-07 2023-11-24 浙江大学 一种模块化组装双组分纳米颗粒制备方法及其应用
CN115645417A (zh) * 2022-10-17 2023-01-31 武汉轻工大学 鹅去氧胆酸在制备治疗或预防猪病毒性腹泻的药物或饲料添加剂中的应用
CN117143888B (zh) * 2023-08-29 2024-08-13 上海杰威医药科技有限公司 一种猪圆环病毒2a、2b、2d型三价病毒样颗粒疫苗及其制备方法和应用
CN117965585B (zh) * 2024-03-29 2024-07-16 中国农业科学院哈尔滨兽医研究所(中国动物卫生与流行病学中心哈尔滨分中心) 猪圆环病毒2d型佐剂嵌合病毒样颗粒疫苗及其制备方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030096377A1 (en) * 2001-06-28 2003-05-22 Virginia Tech Intellectual Properties, Inc. Differential PCR-RFLP assay for detecting and distinguishing between nonpathogenic PCV-1 and pathogenic PCV-2
US7833707B2 (en) * 2004-12-30 2010-11-16 Boehringer Ingelheim Vetmedica, Inc. Methods of overexpression and recovery of porcine circovirus type 2 ORF2
TWI398272B (zh) 2005-03-08 2013-06-11 Intervet Int Bv 化學定義的安定劑
ES2425228T3 (es) 2005-09-09 2013-10-14 Intervet International B.V. Vacuna contra el PCV-2
GB0712160D0 (en) * 2007-06-22 2007-08-01 Univ Ghent Methods and compositions in the treatment of procine circoviral infection
US20100150959A1 (en) 2008-12-15 2010-06-17 Vectogen Pty Ltd. PCV 2-Based Methods and Compositions for the Treatment of Pigs
TW201043267A (en) 2009-03-19 2010-12-16 Intervet Int Bv In situ constituting a vaccine for administration to a predetermined herd of animals
EP3587439A3 (en) * 2013-03-01 2020-03-11 Boehringer Ingelheim Animal Health USA Inc. Improved quantification of vaccine compositions
CN105579060B (zh) * 2013-09-25 2021-03-16 硕腾服务有限责任公司 Pcv2b趋异株疫苗组合物以及使用方法
MX373884B (es) * 2013-10-02 2020-03-26 Boehringer Ingelheim Animal Health Usa Inc Variante de la proteína pcv2 orf2 y partículas similares al virus compuestas por ésta.
BR112017012220A2 (pt) 2014-12-11 2018-01-30 Intervet Int Bv processos para preparar uma composição de antígeno e uma vacina, composição de antígeno, vacina, usos de uma composição de antígeno e de uma vacina, e, método para prevenção ou redução de infecção por um vírus em suíno e sinais associados à doença.
CN104984335A (zh) * 2015-07-14 2015-10-21 浙江诺倍威生物技术有限公司 猪圆环病毒双亚型orf2共表达载体构建及疫苗制备
US11077181B2 (en) 2017-08-03 2021-08-03 Intervet Inc. Vaccine comprising a PCV2 ORF2 protein of genotype 2b
CN111212847B (zh) * 2017-10-17 2024-01-30 英特维特国际股份有限公司 PCV2b ORF2蛋白在昆虫细胞中的重组表达

Also Published As

Publication number Publication date
ES2962449T3 (es) 2024-03-19
CN111212847B (zh) 2024-01-30
CA3078281C (en) 2023-02-21
US20200299726A1 (en) 2020-09-24
JP2020537651A (ja) 2020-12-24
CN111212847A (zh) 2020-05-29
DK3697804T5 (da) 2024-08-26
US11279952B2 (en) 2022-03-22
CA3078281A1 (en) 2019-04-25
BR112020007498A2 (pt) 2020-10-06
RU2020115868A3 (da) 2021-11-18
PL3697804T3 (pl) 2024-01-03
EP3697804A1 (en) 2020-08-26
EP3697804B1 (en) 2023-08-23
KR102480771B1 (ko) 2022-12-23
HUE064720T2 (hu) 2024-04-28
MX385295B (es) 2025-03-18
JP2022065059A (ja) 2022-04-26
MX2020003626A (es) 2021-08-12
RU2020115868A (ru) 2021-11-18
WO2019076864A1 (en) 2019-04-25
KR20200066700A (ko) 2020-06-10
JP7422795B2 (ja) 2024-01-26
JP7062760B2 (ja) 2022-05-06

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