DK3733865T3 - Fremgangsmåde til opnåelse af encellet mrna-sekvens - Google Patents
Fremgangsmåde til opnåelse af encellet mrna-sekvensInfo
- Publication number
- DK3733865T3 DK3733865T3 DK18896527.1T DK18896527T DK3733865T3 DK 3733865 T3 DK3733865 T3 DK 3733865T3 DK 18896527 T DK18896527 T DK 18896527T DK 3733865 T3 DK3733865 T3 DK 3733865T3
- Authority
- DK
- Denmark
- Prior art keywords
- mrna sequence
- obtaining single
- cell mrna
- cell
- obtaining
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2017119342 | 2017-12-28 | ||
| PCT/CN2018/124293 WO2019129133A1 (zh) | 2017-12-28 | 2018-12-27 | 一种获得单细胞mRNA序列的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3733865T3 true DK3733865T3 (da) | 2026-01-12 |
Family
ID=67066601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18896527.1T DK3733865T3 (da) | 2017-12-28 | 2018-12-27 | Fremgangsmåde til opnåelse af encellet mrna-sekvens |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US11718872B2 (da) |
| EP (1) | EP3733865B1 (da) |
| CN (1) | CN111801428B (da) |
| DK (1) | DK3733865T3 (da) |
| WO (1) | WO2019129133A1 (da) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114958996B (zh) * | 2021-05-12 | 2022-12-20 | 浙江大学 | 一种超高通量单细胞测序试剂组合 |
| CN114854827B (zh) * | 2022-05-18 | 2026-02-06 | 苏州极客基因科技有限公司 | 一种用于富集mRNA的磁珠复合物及其应用 |
| CN115198000B (zh) * | 2022-07-21 | 2023-07-21 | 北京寻因生物科技有限公司 | 一种单细胞全序列转录组文库构建的方法 |
| CN115820802A (zh) * | 2022-10-31 | 2023-03-21 | 北京百迈客生物科技有限公司 | 一种单细胞全长转录组文库的构建方法及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9174216B2 (en) * | 2013-03-13 | 2015-11-03 | DeNovo Science, Inc. | System for capturing and analyzing cells |
| US9304065B2 (en) * | 2012-02-29 | 2016-04-05 | Fluidigm Corporation | Methods, systems and devices for multiple single-cell capturing and processing using microfluidics |
| US20160122753A1 (en) * | 2013-06-12 | 2016-05-05 | Tarjei Mikkelsen | High-throughput rna-seq |
| US10975371B2 (en) * | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| US10017759B2 (en) * | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
| CN104032377A (zh) * | 2014-06-30 | 2014-09-10 | 北京诺禾致源生物信息科技有限公司 | 单细胞转录组测序文库的构建方法及其应用 |
| US10059990B2 (en) * | 2015-04-14 | 2018-08-28 | Massachusetts Institute Of Technology | In situ nucleic acid sequencing of expanded biological samples |
| CN106498040B (zh) * | 2016-10-12 | 2018-10-23 | 浙江大学 | 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法 |
| CN106754904B (zh) * | 2016-12-21 | 2019-03-15 | 南京诺唯赞生物科技有限公司 | 一种cDNA的特异性分子标签及其应用 |
| CN107502607A (zh) * | 2017-06-20 | 2017-12-22 | 浙江大学 | 一种大量组织、细胞样本mRNA的分子条形码标记、文库构建、测序的方法 |
-
2018
- 2018-12-27 WO PCT/CN2018/124293 patent/WO2019129133A1/zh not_active Ceased
- 2018-12-27 EP EP18896527.1A patent/EP3733865B1/en active Active
- 2018-12-27 CN CN201880083669.8A patent/CN111801428B/zh active Active
- 2018-12-27 DK DK18896527.1T patent/DK3733865T3/da active
-
2020
- 2020-06-23 US US16/909,366 patent/US11718872B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3733865A4 (en) | 2021-09-08 |
| EP3733865A1 (en) | 2020-11-04 |
| CN111801428A (zh) | 2020-10-20 |
| EP3733865B1 (en) | 2025-10-15 |
| CN111801428B (zh) | 2023-05-23 |
| US20200318181A1 (en) | 2020-10-08 |
| US11718872B2 (en) | 2023-08-08 |
| WO2019129133A1 (zh) | 2019-07-04 |
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