DK3856928T3 - Fremgangsmåde til karakterisering af modifikationer forårsaget af anvendelsen af designernukleaser - Google Patents

Fremgangsmåde til karakterisering af modifikationer forårsaget af anvendelsen af designernukleaser Download PDF

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Publication number
DK3856928T3
DK3856928T3 DK19769176.9T DK19769176T DK3856928T3 DK 3856928 T3 DK3856928 T3 DK 3856928T3 DK 19769176 T DK19769176 T DK 19769176T DK 3856928 T3 DK3856928 T3 DK 3856928T3
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DK
Denmark
Prior art keywords
procedure
modifications caused
designer nucleases
characterizing
characterizing modifications
Prior art date
Application number
DK19769176.9T
Other languages
English (en)
Inventor
Gianni Monaco
Melanie Börries
Geoffroy Andrieux
Toni Cathomen
Giandomenico Turchiano
Georges Blattner
Original Assignee
Univ Freiburg Albert Ludwigs
Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts
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Application filed by Univ Freiburg Albert Ludwigs, Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts filed Critical Univ Freiburg Albert Ludwigs
Application granted granted Critical
Publication of DK3856928T3 publication Critical patent/DK3856928T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/30Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301Endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/119Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pens And Brushes (AREA)
  • Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)
  • Enzymes And Modification Thereof (AREA)
DK19769176.9T 2018-09-25 2019-09-19 Fremgangsmåde til karakterisering af modifikationer forårsaget af anvendelsen af designernukleaser DK3856928T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18196438.8A EP3628748A1 (en) 2018-09-25 2018-09-25 Method for characterization of modifications caused by the use of designer nucleases
PCT/EP2019/075101 WO2020064478A1 (en) 2018-09-25 2019-09-19 Method for characterization of modifications caused by the use of designer nucleases

Publications (1)

Publication Number Publication Date
DK3856928T3 true DK3856928T3 (da) 2023-01-30

Family

ID=63794292

Family Applications (1)

Application Number Title Priority Date Filing Date
DK19769176.9T DK3856928T3 (da) 2018-09-25 2019-09-19 Fremgangsmåde til karakterisering af modifikationer forårsaget af anvendelsen af designernukleaser

Country Status (8)

Country Link
US (1) US11319580B2 (da)
EP (2) EP3628748A1 (da)
JP (2) JP2022512530A (da)
KR (1) KR102899840B1 (da)
DK (1) DK3856928T3 (da)
ES (1) ES2937986T3 (da)
LT (1) LT3856928T (da)
WO (1) WO2020064478A1 (da)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL298058A (en) * 2020-05-11 2023-01-01 Univ Bar Ilan Method and system for determining effect of nucleic acid editing
CN112708662B (zh) * 2020-12-28 2021-08-31 阅尔基因技术(苏州)有限公司 抑制生物dna样本中非目标区域的核苷酸组合物及其应用
CN114032292B (zh) * 2021-12-17 2024-04-12 北京大学 一种绝对定量细胞内dna双链断裂数量的方法及应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107109486B (zh) 2014-11-14 2021-08-13 基础科学研究院 用于检测基因组中遗传剪刀的脱靶位点的方法
EP3221813A4 (en) * 2014-11-20 2018-04-18 Children's Medical Center Corporation Methods relating to the detection of recurrent and non-specific double strand breaks in the genome
WO2018091971A1 (en) * 2016-11-15 2018-05-24 Genomic Vision Method for the monitoring of modified nucleases induced-gene editing events by molecular combing
JP7229923B2 (ja) 2017-01-06 2023-02-28 エディタス・メディシン、インコーポレイテッド ヌクレアーゼ切断を評価する方法

Also Published As

Publication number Publication date
JP2024113001A (ja) 2024-08-21
LT3856928T (lt) 2023-02-27
EP3856928A1 (en) 2021-08-04
US11319580B2 (en) 2022-05-03
EP3856928B1 (en) 2022-11-09
JP2022512530A (ja) 2022-02-07
ES2937986T3 (es) 2023-04-03
EP3628748A1 (en) 2020-04-01
KR102899840B1 (ko) 2025-12-11
US20210317514A1 (en) 2021-10-14
KR20210065085A (ko) 2021-06-03
WO2020064478A1 (en) 2020-04-02

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