DK9787A - Bakterielle enzymer - Google Patents

Bakterielle enzymer

Info

Publication number
DK9787A
DK9787A DK009787A DK9787A DK9787A DK 9787 A DK9787 A DK 9787A DK 009787 A DK009787 A DK 009787A DK 9787 A DK9787 A DK 9787A DK 9787 A DK9787 A DK 9787A
Authority
DK
Denmark
Prior art keywords
enzymes
serratia species
nuclease
bacterial enzymes
organism
Prior art date
Application number
DK009787A
Other languages
English (en)
Other versions
DK9787D0 (da
DK173445B1 (da
Inventor
Soeren Molin
Michael Givskov
Erik Riise
Original Assignee
Benzon As Alfred
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK210085A external-priority patent/DK210085D0/da
Priority claimed from DK606085A external-priority patent/DK606085D0/da
Application filed by Benzon As Alfred filed Critical Benzon As Alfred
Priority to DK198700097A priority Critical patent/DK173445B1/da
Publication of DK9787D0 publication Critical patent/DK9787D0/da
Publication of DK9787A publication Critical patent/DK9787A/da
Application granted granted Critical
Publication of DK173445B1 publication Critical patent/DK173445B1/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Compounds Of Unknown Constitution (AREA)
DK198700097A 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba DK173445B1 (da)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK198700097A DK173445B1 (da) 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
DK210085 1985-05-10
DK210085A DK210085D0 (da) 1985-05-10 1985-05-10 Fremgangsmaade til fremstilling af bakterielle enzymer
DK606085A DK606085D0 (da) 1985-12-23 1985-12-23 Fremgangsmaade til fremstilling af et bakterielt enzym
DK606085 1985-12-23
DK8600051 1986-05-09
PCT/DK1986/000051 WO1986006743A1 (en) 1985-05-10 1986-05-09 Bacterial enzymes
DK9787 1987-01-09
DK198700097A DK173445B1 (da) 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Publications (3)

Publication Number Publication Date
DK9787D0 DK9787D0 (da) 1987-01-09
DK9787A true DK9787A (da) 1987-01-09
DK173445B1 DK173445B1 (da) 2000-11-06

Family

ID=26066359

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198700097A DK173445B1 (da) 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Country Status (13)

Country Link
EP (1) EP0229866B1 (da)
KR (1) KR930002887B1 (da)
CN (1) CN1008377B (da)
AT (1) ATE83264T1 (da)
AU (2) AU594062B2 (da)
CA (1) CA1327328C (da)
DE (2) DE3687266T2 (da)
DK (1) DK173445B1 (da)
ES (2) ES8800346A1 (da)
FI (1) FI870052L (da)
IE (1) IE59296B1 (da)
IL (1) IL78703A (da)
WO (1) WO1986006743A1 (da)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3939771A1 (de) * 1989-12-01 1991-06-06 Behringwerke Ag Verfahren zur biologischen inaktivierung von nukleinsaeuren
IL103846A0 (en) * 1991-11-25 1993-04-04 Tanabe Seiyaku Co Gene coding for esterase and novel microorganism containing said gene
US5834233A (en) * 1992-04-06 1998-11-10 Gx Biosystems A/S Method of limiting the survival of genetically engineered microorganisms in their enivronment
CH682541A5 (de) * 1992-06-23 1993-10-15 Urs Viktor Dr Wirth Biochemische Inaktivierung von RNA-Viren.
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
US5968777A (en) * 1996-04-18 1999-10-19 Smithkline Beecham Corporation DNA encoding seryl t-RNA synthetase
SI1539798T1 (sl) 2002-09-06 2011-03-31 Genentech Inc Postopek za proteinsko ekstrakcijo
WO2008108006A1 (ja) 2007-03-07 2008-09-12 On-Chip Cellomics Consortium 細胞分離方法ならびに細胞検査方法とその試薬キット
EP2108956A1 (en) 2008-04-07 2009-10-14 Erasmus University Medical Center Rotterdam Improved methods and kits for detecting tumor-specific fusion proteins
WO2009153563A1 (en) 2008-06-18 2009-12-23 Oxford Biomedica (Uk) Limited Virus purification
DK2462224T3 (da) 2009-08-03 2017-09-04 C-Lecta Gmbh Fremgangsmåde til fremstilling af nukleaser af et gram negativt bakterie under udnyttelse af en gram positiv ekspressionsvært
AU2013329110B2 (en) 2012-10-12 2017-05-04 Sage Science, Inc. Side-eluting molecular fractionator
CN103205448B (zh) * 2013-02-18 2016-03-23 徐东 一种裂解时自动降解核酸的大肠杆菌制备的方法
CN104099310B (zh) * 2013-04-12 2021-06-18 杭州俊丰生物工程有限公司 一种重组核酸酶及其制备方法
US10131901B2 (en) 2014-10-15 2018-11-20 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
EP3286324B1 (en) 2015-04-20 2020-05-20 Qiagen GmbH Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample
AU2016357319B2 (en) 2015-11-20 2022-03-10 Sage Science, Inc. Preparative electrophoretic method for targeted purification of genomic DNA fragments
WO2017139669A1 (en) * 2016-02-11 2017-08-17 Sage Science, Inc. Workflows for enzymatic nucleic acid modification
WO2018187779A1 (en) 2017-04-07 2018-10-11 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic dna purification
MX2020012295A (es) 2018-05-15 2021-03-31 Flagship Pioneering Innovations V Inc Composiciones de fusosoma y usos de estas.
WO2020014209A1 (en) 2018-07-09 2020-01-16 Flagship Pioneering Innovations V, Inc. Fusosome compositions and uses thereof
CA3120082A1 (en) 2018-11-14 2020-05-22 Flagship Pioneering Innovations V, Inc. Fusosome compositions for cns delivery
WO2020102485A1 (en) 2018-11-14 2020-05-22 Flagship Pioneering Innovations V, Inc. Fusosome compositions for hematopoietic stem cell delivery
WO2020102503A2 (en) 2018-11-14 2020-05-22 Flagship Pioneering Innovations V, Inc. Fusosome compositions for t cell delivery
US11926853B2 (en) 2018-12-21 2024-03-12 The Research Foundation For Microbial Diseases Of Osaka University Botulinum toxin producing method
EP3926039A1 (en) 2020-06-17 2021-12-22 AB Enzymes GmbH Use of a nuclease for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5315491A (en) * 1976-07-23 1978-02-13 Rikagaku Kenkyusho Preparation of novel nucleicacidase
US4174400A (en) * 1978-09-13 1979-11-13 Merck & Co., Inc. Anthelmintic benzimidazoles
GB2121054B (en) * 1982-05-25 1986-02-26 Lilly Co Eli Cloning vectors for expression of exogenous protein
JPS59183688A (ja) * 1983-03-31 1984-10-18 Tanabe Seiyaku Co Ltd L−アスパラギン酸の製法
AU3107684A (en) * 1983-07-01 1985-02-07 Celltech Limited Proteins, pharmaceutical compositions, genes, vectors, host organisms and processes for their production
DE3334847A1 (de) * 1983-09-27 1985-04-04 Hoechst Ag, 6230 Frankfurt Immobilisierte 5'-phosphodiesterase, verfahren zu ihrer herstellung und ihre verwendung

Also Published As

Publication number Publication date
DK9787D0 (da) 1987-01-09
DE3687266T2 (de) 1993-04-15
IL78703A0 (en) 1986-08-31
DE229866T1 (de) 1988-01-14
ATE83264T1 (de) 1992-12-15
KR880700069A (ko) 1988-02-15
AU5910086A (en) 1986-12-04
FI870052A7 (fi) 1987-01-07
EP0229866A1 (en) 1987-07-29
EP0229866B1 (en) 1992-12-09
IL78703A (en) 1993-02-21
ES8801946A1 (es) 1988-03-01
KR930002887B1 (ko) 1993-04-12
FI870052A0 (fi) 1987-01-07
CN1008377B (zh) 1990-06-13
DE3687266D1 (de) 1993-01-21
CN86104387A (zh) 1987-04-22
WO1986006743A1 (en) 1986-11-20
IE861236L (en) 1986-11-10
ES8800346A1 (es) 1987-11-01
DK173445B1 (da) 2000-11-06
ES557687A0 (es) 1988-03-01
CA1327328C (en) 1994-03-01
AU594062B2 (en) 1990-03-01
FI870052L (fi) 1987-01-07
ES554856A0 (es) 1987-11-01
AU3679389A (en) 1989-11-23
IE59296B1 (en) 1994-02-09

Similar Documents

Publication Publication Date Title
DK9787D0 (da) Bakterielle enzymer
DE69435030D1 (de) Methode zur Detektion eines DNA Zielmoleküls
FI883708A0 (fi) Menetelmä emäksisten proteiinien eristämiseksi proteiiniseoksista, jotka sisältävät tällaisia emäksisiä proteiineja
DK239383D0 (da) Mikrobiologisk fremstillede alfa-og beta-interferoner, dna-sekvenser, der koder for disse interferoner, mikroorganismer, som indeholder denne genetiske information, og fremgangsmade til deres fremstilling
AU524856B2 (en) Method of making a selected protein
FI843529A0 (fi) Foerfarande foer detektering, identifiering och kvantitering av organismer och virus.
NO874979L (no) Fremgangsmaate for isolering og rensing av nukleinsyrer fra biologiske proever.
ES2097115T3 (es) Procedimiento para la integracion de un gen elegido en el cromosoma de una bacteria y bacteria obtenida por dicho procedimiento.
NZ208806A (en) Genetic engineering of industrial microorganism species: readily-transformable host used as intermediate in transfer of dna to the industrial species; plasmids
AU7225591A (en) Nucleic acid enzymes for cleaving dna
DE3689572D1 (de) Verfahren zur Isolierung von Genen zur Biosynthese von Antibiotika.
MX9203379A (es) Proteinas producidas por los linfocitos humanos, secuencia de adn que codifica para estas proteinas y composiciones farmaceuticas que las contienen.
AU1941092A (en) Dna sequences to target proteins to the mammary gland for efficient secretion
FI885504A0 (fi) Vaskulaera antikoagulerande proteiner, dessa koderande dna, deras framstaellningsfoerfarande och deras anvaendning.
BR8205954A (pt) Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
ATE89318T1 (de) Verfahren zur gentechnologischengewinnung von proteinen unter verwendung gramnegativer wirtzellen.
ATE89317T1 (de) Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten.
FI102544B1 (fi) Menetelmä E.coli-kannan valmistamiseksi
AU3107684A (en) Proteins, pharmaceutical compositions, genes, vectors, host organisms and processes for their production
DK560686A (da) Fremgangsmaade til fremstilling af proteiner ved hjaelp af vaertsorganismer, fortrinsvis maelkesyrebakterier
DE3673436D1 (de) Gene fuer biologisch aktive proteine.
IT8119180A0 (it) Polipeptide prodotto microbiologicamente con la sequenza di aminoacidi della proinsulina di primati, dna e plasmidi che codificano per questa sequenza, microorganismi che contengono queste informazioni genetiche e processo per la loro preparazione.
ATE339186T1 (de) Methode zur herstellung von mikropartikeln enthaltend biologisches material
GB8828024D0 (en) Dna having genetic information of l-a-glycerophosphate oxidase & application thereof
ES8702925A1 (es) Un procedimiento para preparar un polipeptido que tiene ac- tividad de factor de crecimiento

Legal Events

Date Code Title Description
B1 Patent granted (law 1993)
PUP Patent expired