EA200200057A1 - Ген streptomyces avermitilis, контролирующий соотношение авермектинов b2:b1 - Google Patents

Ген streptomyces avermitilis, контролирующий соотношение авермектинов b2:b1

Info

Publication number
EA200200057A1
EA200200057A1 EA200200057A EA200200057A EA200200057A1 EA 200200057 A1 EA200200057 A1 EA 200200057A1 EA 200200057 A EA200200057 A EA 200200057A EA 200200057 A EA200200057 A EA 200200057A EA 200200057 A1 EA200200057 A1 EA 200200057A1
Authority
EA
Eurasian Patent Office
Prior art keywords
avermectines
monitoring
relation
streptomyces avermitilis
avermitilis
Prior art date
Application number
EA200200057A
Other languages
English (en)
Other versions
EA004569B1 (ru
Inventor
Ким Джонелл Статзман-Энгволл
Original Assignee
Пфайзер Продактс Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Пфайзер Продактс Инк. filed Critical Пфайзер Продактс Инк.
Publication of EA200200057A1 publication Critical patent/EA200200057A1/ru
Publication of EA004569B1 publication Critical patent/EA004569B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C12P19/623—Avermectin; Milbemycin; Ivermectin; C-076

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Настоящее изобретение относится к полинуклеотидным молекулам, содержащим нуклеотидные последовательности, кодирующие продукт гена aveC, причем эти полинуклеотидные молекулы можно использовать для изменения соотношения или количества авермектинов классов 2:1, продуцируемых в ферментационных культурах S. avermitilis. Кроме того, настоящее изобретение относится к векторам, клеткам-хозяевам и мутантным штаммам S. avermitilis, в которых ген aveC инактивирован или мутирован таким образом, чтобы изменить соотношение или количество продуцируемых авермектинов классов 2:1.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200200057A 1999-08-12 2000-07-19 Ген streptomyces avermitilis, контролирующий соотношение авермектинов b2:b1 EA004569B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/372,934 US6248579B1 (en) 1998-02-13 1999-08-12 Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
PCT/IB2000/000996 WO2001012822A1 (en) 1999-08-12 2000-07-19 Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins

Publications (2)

Publication Number Publication Date
EA200200057A1 true EA200200057A1 (ru) 2002-06-27
EA004569B1 EA004569B1 (ru) 2004-06-24

Family

ID=23470234

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200200057A EA004569B1 (ru) 1999-08-12 2000-07-19 Ген streptomyces avermitilis, контролирующий соотношение авермектинов b2:b1

Country Status (37)

Country Link
US (8) US6248579B1 (ru)
EP (1) EP1200605B1 (ru)
JP (2) JP3688640B2 (ru)
KR (1) KR100486006B1 (ru)
CN (1) CN1186447C (ru)
AP (2) AP2002002419A0 (ru)
AR (1) AR021450A1 (ru)
AT (1) ATE283363T1 (ru)
AU (1) AU779756B2 (ru)
BG (1) BG106478A (ru)
BR (1) BR0013093A (ru)
CA (1) CA2381427A1 (ru)
CO (1) CO5290296A1 (ru)
CZ (1) CZ2002297A3 (ru)
DE (1) DE60016231T2 (ru)
EA (1) EA004569B1 (ru)
ES (1) ES2231214T3 (ru)
GT (2) GT200000138CA (ru)
HK (1) HK1046536B (ru)
HR (1) HRP20020131A2 (ru)
HU (1) HUP0202517A2 (ru)
IL (1) IL147562A0 (ru)
IS (1) IS6249A (ru)
MA (1) MA26811A1 (ru)
MX (1) MXPA02001514A (ru)
NO (1) NO20020665L (ru)
OA (1) OA11997A (ru)
PA (1) PA8499201A1 (ru)
PE (1) PE20010697A1 (ru)
PL (1) PL353770A1 (ru)
PT (1) PT1200605E (ru)
SK (1) SK1382002A3 (ru)
TN (1) TNSN00170A1 (ru)
UY (1) UY26287A1 (ru)
WO (1) WO2001012822A1 (ru)
YU (1) YU8402A (ru)
ZA (1) ZA200201071B (ru)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2380872C (en) * 1999-08-12 2007-11-27 Yan Chen Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins
ATE373644T1 (de) 2002-02-05 2007-10-15 Bristol Myers Squibb Co N-substituierte 3-hydroxy-4-pyridinone und diese enthaltende pharmazeutika
DK1476539T3 (da) 2002-02-12 2009-05-11 Pfizer Prod Inc Streptomyces avermitilis gen dirigerende forholdet af B2:B1 avermectiner
US7943160B2 (en) * 2002-05-09 2011-05-17 Scimetrics Limited Corp. Pest control methods
EP1597271B1 (de) * 2003-01-31 2013-03-06 Max-Delbrück-Centrum Für Molekulare Medizin Peptide gegen kälteunverträglichkeit hervorrufende autoantikörper und ihre verwendung
KR100759130B1 (ko) 2005-02-12 2007-09-19 휴메드 주식회사 항 인테그린 항체 코팅스텐트 및 그의 제조방법
CN106148216B (zh) * 2015-03-27 2019-06-04 浙江海正药业股份有限公司 一种链霉菌及其生产米尔贝霉素a3的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5238848A (en) 1987-01-23 1993-08-24 Pfizer Inc Cultures for production of avermectins
US5234831A (en) 1987-01-23 1993-08-10 Pfizer Inc Cultures for production of B avermectins
US5525506A (en) 1987-01-23 1996-06-11 Pfizer Inc. Process for production of avermectins and cultures therefor
US5240850A (en) 1987-10-23 1993-08-31 Pfizer Inc. Cultures for production of avermectin aglycones
ES2054822T3 (es) 1987-10-23 1994-08-16 Pfizer Procedimiento para la produccion de agliconas de avermectinas y sus cultivos.
EP0391594B1 (en) 1989-03-31 1995-10-18 Merck & Co. Inc. Cloning genes from streptomyces avermitilis for avermectin biosynthesis and the methods for their use
US5252474A (en) 1989-03-31 1993-10-12 Merck & Co., Inc. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use
PL181916B1 (en) 1993-07-30 2001-10-31 Pfizer Method of obtaining natural avermectin
JP2761296B2 (ja) * 1993-10-05 1998-06-04 フアイザー・インコーポレイテツド ドラメクチンの製造方法および駆虫性中間体
FI942725A7 (fi) 1993-12-16 1995-06-17 Pfizer Haaraketjuista alfaketohappodenydrogenaasikompleksia koodaavia geenejä Streptomyces avermitiliksesta
EA003905B1 (ru) * 1998-02-13 2003-10-30 Пфайзер Продактс Инк. Ген streptomyces avermitilis, контролирующий соотношение авермектинов b2:b1

Also Published As

Publication number Publication date
BG106478A (bg) 2004-02-27
PA8499201A1 (es) 2001-12-14
US20010016350A1 (en) 2001-08-23
JP2003507018A (ja) 2003-02-25
ATE283363T1 (de) 2004-12-15
US7029887B2 (en) 2006-04-18
US6511841B2 (en) 2003-01-28
US7259241B2 (en) 2007-08-21
ZA200201071B (en) 2003-06-25
JP3688640B2 (ja) 2005-08-31
US6248579B1 (en) 2001-06-19
US20030129636A1 (en) 2003-07-10
HK1046536A1 (en) 2003-01-17
EP1200605B1 (en) 2004-11-24
NO20020665D0 (no) 2002-02-11
MXPA02001514A (es) 2002-07-02
NO20020665L (no) 2002-02-11
WO2001012822A1 (en) 2001-02-22
US20030124607A1 (en) 2003-07-03
US20030134313A1 (en) 2003-07-17
UY26287A1 (es) 2001-03-16
CN1370239A (zh) 2002-09-18
US20010024818A1 (en) 2001-09-27
BR0013093A (pt) 2002-08-13
MA26811A1 (fr) 2004-12-20
KR20020022809A (ko) 2002-03-27
GT200000138A (es) 2002-02-02
PE20010697A1 (es) 2001-07-05
CZ2002297A3 (cs) 2002-08-14
HUP0202517A2 (hu) 2002-11-28
US20050196777A1 (en) 2005-09-08
JP2005198658A (ja) 2005-07-28
OA11997A (en) 2006-04-18
PL353770A1 (en) 2003-12-01
HRP20020131A2 (en) 2003-12-31
CO5290296A1 (es) 2003-06-27
GT200000138CA (es) 2002-02-02
DE60016231D1 (de) 2004-12-30
TNSN00170A1 (fr) 2005-11-10
AP2002002417A0 (en) 2002-03-31
AP2002002419A0 (en) 2002-03-31
KR100486006B1 (ko) 2005-05-03
ES2231214T3 (es) 2005-05-16
IL147562A0 (en) 2002-08-14
AR021450A1 (es) 2002-07-17
IS6249A (is) 2002-01-25
CN1186447C (zh) 2005-01-26
US20030129635A1 (en) 2003-07-10
US6833263B2 (en) 2004-12-21
EA004569B1 (ru) 2004-06-24
PT1200605E (pt) 2005-04-29
US6509159B2 (en) 2003-01-21
DE60016231T2 (de) 2005-12-08
SK1382002A3 (en) 2002-11-06
CA2381427A1 (en) 2001-02-22
EP1200605A1 (en) 2002-05-02
AU779756B2 (en) 2005-02-10
AU5702100A (en) 2001-03-13
HK1046536B (zh) 2005-05-13
YU8402A (sh) 2006-03-03

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