EA200200057A1 - Ген streptomyces avermitilis, контролирующий соотношение авермектинов b2:b1 - Google Patents

Ген streptomyces avermitilis, контролирующий соотношение авермектинов b2:b1

Info

Publication number
EA200200057A1
EA200200057A1 EA200200057A EA200200057A EA200200057A1 EA 200200057 A1 EA200200057 A1 EA 200200057A1 EA 200200057 A EA200200057 A EA 200200057A EA 200200057 A EA200200057 A EA 200200057A EA 200200057 A1 EA200200057 A1 EA 200200057A1
Authority
EA
Eurasian Patent Office
Prior art keywords
avermectines
monitoring
relation
streptomyces avermitilis
avermitilis
Prior art date
Application number
EA200200057A
Other languages
English (en)
Other versions
EA004569B1 (ru
Inventor
Ким Джонелл Статзман-Энгволл
Original Assignee
Пфайзер Продактс Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Пфайзер Продактс Инк. filed Critical Пфайзер Продактс Инк.
Publication of EA200200057A1 publication Critical patent/EA200200057A1/ru
Publication of EA004569B1 publication Critical patent/EA004569B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C12P19/623—Avermectin; Milbemycin; Ivermectin; C-076

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Настоящее изобретение относится к полинуклеотидным молекулам, содержащим нуклеотидные последовательности, кодирующие продукт гена aveC, причем эти полинуклеотидные молекулы можно использовать для изменения соотношения или количества авермектинов классов 2:1, продуцируемых в ферментационных культурах S. avermitilis. Кроме того, настоящее изобретение относится к векторам, клеткам-хозяевам и мутантным штаммам S. avermitilis, в которых ген aveC инактивирован или мутирован таким образом, чтобы изменить соотношение или количество продуцируемых авермектинов классов 2:1.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200200057A 1999-08-12 2000-07-19 Ген streptomyces avermitilis, контролирующий соотношение авермектинов b2:b1 EA004569B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/372,934 US6248579B1 (en) 1998-02-13 1999-08-12 Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
PCT/IB2000/000996 WO2001012822A1 (en) 1999-08-12 2000-07-19 Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins

Publications (2)

Publication Number Publication Date
EA200200057A1 true EA200200057A1 (ru) 2002-06-27
EA004569B1 EA004569B1 (ru) 2004-06-24

Family

ID=23470234

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200200057A EA004569B1 (ru) 1999-08-12 2000-07-19 Ген streptomyces avermitilis, контролирующий соотношение авермектинов b2:b1

Country Status (37)

Country Link
US (8) US6248579B1 (ru)
EP (1) EP1200605B1 (ru)
JP (2) JP3688640B2 (ru)
KR (1) KR100486006B1 (ru)
CN (1) CN1186447C (ru)
AP (2) AP2002002417A0 (ru)
AR (1) AR021450A1 (ru)
AT (1) ATE283363T1 (ru)
AU (1) AU779756B2 (ru)
BG (1) BG106478A (ru)
BR (1) BR0013093A (ru)
CA (1) CA2381427A1 (ru)
CO (1) CO5290296A1 (ru)
CZ (1) CZ2002297A3 (ru)
DE (1) DE60016231T2 (ru)
EA (1) EA004569B1 (ru)
ES (1) ES2231214T3 (ru)
GT (2) GT200000138A (ru)
HK (1) HK1046536B (ru)
HR (1) HRP20020131A2 (ru)
HU (1) HUP0202517A2 (ru)
IL (1) IL147562A0 (ru)
IS (1) IS6249A (ru)
MA (1) MA26811A1 (ru)
MX (1) MXPA02001514A (ru)
NO (1) NO20020665L (ru)
OA (1) OA11997A (ru)
PA (1) PA8499201A1 (ru)
PE (1) PE20010697A1 (ru)
PL (1) PL353770A1 (ru)
PT (1) PT1200605E (ru)
SK (1) SK1382002A3 (ru)
TN (1) TNSN00170A1 (ru)
UY (1) UY26287A1 (ru)
WO (1) WO2001012822A1 (ru)
YU (1) YU8402A (ru)
ZA (1) ZA200201071B (ru)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0013119B8 (pt) * 1999-08-12 2021-05-25 Pah Usa 15 Llc molécula polinucleotídica isolada, vetor recombinante purificado, célula hospedeira procariótica e célula de streptomyces avermitilis mutada, método para obtenção de uma nova cepa de s. avermitilis e processo para produção de avermectinas
JP2005516987A (ja) 2002-02-05 2005-06-09 ブリストル−マイヤーズ　スクイブ　カンパニー Ｎ−置換された３−ヒドロキシ−４−ピリジノン化合物およびそれを含む医薬
PT1476539E (pt) 2002-02-12 2009-04-09 Pfizer Prod Inc Gene streptomyces avermitilis que dirige a proporção de avermectinas b2:b1
US7943160B2 (en) * 2002-05-09 2011-05-17 Scimetrics Limited Corp. Pest control methods
US8440609B2 (en) * 2003-01-31 2013-05-14 Gerd Wallukat Peptides against autoantibodies causing intolerance to cold and use thereof
KR100759130B1 (ko) 2005-02-12 2007-09-19 휴메드 주식회사 항 인테그린 항체 코팅스텐트 및 그의 제조방법
CN106148216B (zh) 2015-03-27 2019-06-04 浙江海正药业股份有限公司 一种链霉菌及其生产米尔贝霉素a3的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234831A (en) 1987-01-23 1993-08-10 Pfizer Inc Cultures for production of B avermectins
US5525506A (en) 1987-01-23 1996-06-11 Pfizer Inc. Process for production of avermectins and cultures therefor
US5238848A (en) 1987-01-23 1993-08-24 Pfizer Inc Cultures for production of avermectins
DE3881147T2 (de) 1987-10-23 1993-09-02 Pfizer Verfahren zur herstellung von aglykonen von avermectin und sie enthaltende kulturen.
US5240850A (en) 1987-10-23 1993-08-31 Pfizer Inc. Cultures for production of avermectin aglycones
ES2079436T3 (es) 1989-03-31 1996-01-16 Merck & Co Inc Clonacion de genes de streptomyces avermitilis para biosintesis de avermectinas y procedimientos para su uso.
US5252474A (en) 1989-03-31 1993-10-12 Merck & Co., Inc. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use
JP2807348B2 (ja) 1993-07-30 1998-10-08 ファイザー・インコーポレーテッド ストレプトミセス・アベルミティリスからの分岐状鎖α−ケト酸デヒドロゲナーゼ複合体をコードする遺伝子
RU2120943C1 (ru) * 1993-10-05 1998-10-27 Пфайзер Инк. Производные дорамектина, способы их получения, способы лечения
FI942725L (fi) 1993-12-16 1995-06-17 Pfizer Haaraketjuista alfaketohappodenydrogenaasikompleksia koodaavia geenejä Streptomyces avermitiliksesta
CN1521180A (zh) * 1998-02-13 2004-08-18 �Ʒ� 介导除虫菌素b2：b1比例的除虫链霉菌基因

Also Published As

Publication number Publication date
HK1046536A1 (en) 2003-01-17
DE60016231T2 (de) 2005-12-08
US20050196777A1 (en) 2005-09-08
ES2231214T3 (es) 2005-05-16
AU779756B2 (en) 2005-02-10
CN1186447C (zh) 2005-01-26
AP2002002419A0 (en) 2002-03-31
NO20020665D0 (no) 2002-02-11
US7029887B2 (en) 2006-04-18
KR100486006B1 (ko) 2005-05-03
US20010016350A1 (en) 2001-08-23
HRP20020131A2 (en) 2003-12-31
AR021450A1 (es) 2002-07-17
UY26287A1 (es) 2001-03-16
HK1046536B (zh) 2005-05-13
US20030134313A1 (en) 2003-07-17
CA2381427A1 (en) 2001-02-22
JP3688640B2 (ja) 2005-08-31
EP1200605B1 (en) 2004-11-24
CO5290296A1 (es) 2003-06-27
US20030129636A1 (en) 2003-07-10
PE20010697A1 (es) 2001-07-05
OA11997A (en) 2006-04-18
GT200000138A (es) 2002-02-02
US6833263B2 (en) 2004-12-21
PT1200605E (pt) 2005-04-29
PA8499201A1 (es) 2001-12-14
AP2002002417A0 (en) 2002-03-31
EA004569B1 (ru) 2004-06-24
US6248579B1 (en) 2001-06-19
IS6249A (is) 2002-01-25
BR0013093A (pt) 2002-08-13
IL147562A0 (en) 2002-08-14
US6509159B2 (en) 2003-01-21
US6511841B2 (en) 2003-01-28
TNSN00170A1 (fr) 2005-11-10
HUP0202517A2 (hu) 2002-11-28
US20030124607A1 (en) 2003-07-03
JP2005198658A (ja) 2005-07-28
JP2003507018A (ja) 2003-02-25
CN1370239A (zh) 2002-09-18
ATE283363T1 (de) 2004-12-15
US7259241B2 (en) 2007-08-21
DE60016231D1 (de) 2004-12-30
KR20020022809A (ko) 2002-03-27
WO2001012822A1 (en) 2001-02-22
EP1200605A1 (en) 2002-05-02
SK1382002A3 (en) 2002-11-06
MA26811A1 (fr) 2004-12-20
MXPA02001514A (es) 2002-07-02
NO20020665L (no) 2002-02-11
BG106478A (bg) 2004-02-27
GT200000138CA (es) 2002-02-02
ZA200201071B (en) 2003-06-25
AU5702100A (en) 2001-03-13
CZ2002297A3 (cs) 2002-08-14
PL353770A1 (en) 2003-12-01
US20010024818A1 (en) 2001-09-27
YU8402A (sh) 2006-03-03
US20030129635A1 (en) 2003-07-10

Similar Documents

Publication Publication Date Title
BR9712878A (pt) Variante de enzima subtilase, processos para a identificação de uma variante de protease apresentando estabilidade autoproteolìtica e paraq a produção de uma enzima subtilase mutante e de uma variante de subtilase, sequência de dna, vetor, célula hospedeira microbiana, composição e uso de uma variante de subtilase.
Foran et al. Functional characterization of a novel “ulvan utilization loci” found in Alteromonas sp. LOR genome
BR9811248B1 (pt) variante de enzima subtilase derivada de uma subtilase originária selecionada a partir do sub-grupo i-s1 ou do sub-grupo i-s2, dita variante tendo melhorado desempenho de lavagem em detergentes em comparação com a subtilase originária, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processo para produzir uma variante, composição, uso de uma variante de subtilase.
BG93814A (bg) Мутантни микробиални алфа-амилази с повишена термична,киселинна и/или алкална устойчивост
AU2002321094A1 (en) A novel group of alpha-amylases and a method for identification and production of novel alpha-amylases
BR9814236A (pt) Enzima subtilase, sua variante, sequência de dna isolada, vetor de expressão, célula microbiana hospedeira, processo para produzir uma subtilase ou uma variante de subtilase, composição, e, uso de uma subtilase ou de uma variante de subtilase.
Stackebrandt et al. Deoxyribonucleic acid homologies and ribosomal ribonucleic acid similarities among sporeforming members of the order Actinomycetales
Hesse et al. Functional and biochemical analysis of Chlamydia trachomatis MurC, an enzyme displaying UDP-N-acetylmuramate: amino acid ligase activity
Colby et al. Insertional inactivation of the Streptococcus mutans dexA (dextranase) gene results in altered adherence and dextran catabolism
MXPA02003133A (es) Moleculas de acido nucleico para la deteccion de bacterias y unidades filogeneticas de bacterias.
BR9911162B1 (pt) vetor, microorganismo transgênico, e, uso de uma seqüência de ácido nucleico.
Wu et al. Emended descriptions of Prevotella denticola, Prevotella loescheii, Prevotella veroralis, and Prevotella melaninogenica
EA200000758A1 (ru) Ген streptomyces avermitilis, изменяющий соотношение в2:в1
BR9811412A (pt) Variante de enzima subtilase tendo melhorado desempenho de lavagem em detergentes, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processos para produzir uma variante e para identificar uma variante de protease, composição, e, uso de uma variante de subtilase
YU8402A (sh) Gen streptomyces avermitilis koji usmerava odnos b2:b1 avermektina
EA200200056A1 (ru) Ген streptomyces avermitilis, направляющий соотношение авермектинов b2:b1
CY1112491T1 (el) Γονιδιο streptomyces avermitilis που ελεγχει την αναλογια αβερμεκτινων β2:β1
Yamamura et al. Cellulomonas algicola sp. nov., an actinobacterium isolated from a freshwater alga
Popoff et al. Taxonomic position of lecithinase-negative strains of Clostridium sordellii
Han et al. Allohahella marinimesophila gen. nov., sp. nov., isolated from seawater and reclassification of Hahella antarctica as Allohahella antarctica comb. nov.
EP1283868A4 (en) ISOLATED GUM OPERON MADE OF XYLLELA FASTIDIOSA, ISOLATED NUCLEIC ACID MOLECULES AND THEIR USE
Callahan et al. Toxicity of Pseudomonas aeruginosa slime
BR9811396A (pt) Variante de enzima subtilase tendo melhorado desempenho de lavagem em detergentes, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processos para produzir uma variante e para identificar uma variante de protease, composição, e, uso de uma variante de subtilase
WO2002086067A3 (en) Mycobacterial sulfation pathway proteins and methods of use thereof
Berber et al. Numerical analysis of SDS-PAGE protein patterns of facultative alkaliphilic Bacillus species isolated from Lake Van, Turkey

Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU

NF4A Restoration of lapsed right to a eurasian patent

Designated state(s): AM AZ BY KZ KG MD TJ TM RU

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU