EA200501774A1 - Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции - Google Patents

Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции

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Publication number
EA200501774A1
EA200501774A1 EA200501774A EA200501774A EA200501774A1 EA 200501774 A1 EA200501774 A1 EA 200501774A1 EA 200501774 A EA200501774 A EA 200501774A EA 200501774 A EA200501774 A EA 200501774A EA 200501774 A1 EA200501774 A1 EA 200501774A1
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EA
Eurasian Patent Office
Prior art keywords
cells
cultures
products
cultivation
increasing
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EA200501774A
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English (en)
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EA008670B1 (ru
Inventor
Кристофер Адам Яллоп
Original Assignee
Круселл Холланд Б.В.
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Application filed by Круселл Холланд Б.В. filed Critical Круселл Холланд Б.В.
Publication of EA200501774A1 publication Critical patent/EA200501774A1/ru
Publication of EA008670B1 publication Critical patent/EA008670B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Neurology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Toxicology (AREA)
  • Neurosurgery (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Изобретение предлагает способы культивирования клеток, происходящих из эмбриональных клеток-ретинобластов, иммортализованных аденовирусными последовательностями E1, предпочтительно клеток PER.C6™, для улучшения выхода продуктов таких клеток. Предлагаются стратегии питания таких клеток и культур при высоких плотностях, дающие высокий выход продуктов, таких как рекомбинантные антитела.
EA200501774A 2003-05-09 2004-05-06 Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции EA008670B1 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP0350155 2003-05-09
EP0350390 2003-09-01
EP0350940 2003-12-04
EP2004050061 2004-01-30
PCT/EP2004/050724 WO2004099396A1 (en) 2003-05-09 2004-05-06 Cultures of e1-immortalized cells and processes for culturing the same to increase product yields therefrom

Publications (2)

Publication Number Publication Date
EA200501774A1 true EA200501774A1 (ru) 2006-04-28
EA008670B1 EA008670B1 (ru) 2007-06-29

Family

ID=33437116

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200501774A EA008670B1 (ru) 2003-05-09 2004-05-06 Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции

Country Status (15)

Country Link
US (4) US7291484B2 (ru)
EP (1) EP1623023B1 (ru)
JP (1) JP4865539B2 (ru)
KR (1) KR101183770B1 (ru)
CN (2) CN101328473B (ru)
AT (1) ATE414144T1 (ru)
AU (1) AU2004236440B2 (ru)
BR (1) BRPI0409895B8 (ru)
CA (1) CA2520891C (ru)
DE (1) DE602004017709D1 (ru)
DK (1) DK1623023T3 (ru)
EA (1) EA008670B1 (ru)
IL (2) IL171680A (ru)
NZ (1) NZ542585A (ru)
WO (1) WO2004099396A1 (ru)

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JP4865539B2 (ja) 2012-02-01
CN101328473A (zh) 2008-12-24
KR101183770B1 (ko) 2012-09-17
CA2520891C (en) 2014-07-08
US20100311160A1 (en) 2010-12-09
CN100383238C (zh) 2008-04-23
AU2004236440A1 (en) 2004-11-18
HK1080115A1 (en) 2006-04-21
WO2004099396A1 (en) 2004-11-18
JP2006525800A (ja) 2006-11-16
US7608431B2 (en) 2009-10-27
EA008670B1 (ru) 2007-06-29
CA2520891A1 (en) 2004-11-18
CN1784489A (zh) 2006-06-07
BRPI0409895A (pt) 2006-05-23
US20070031932A1 (en) 2007-02-08
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ATE414144T1 (de) 2008-11-15
EP1623023B1 (en) 2008-11-12
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KR20060003081A (ko) 2006-01-09
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NZ542585A (en) 2007-11-30
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US8008043B2 (en) 2011-08-30
DE602004017709D1 (de) 2008-12-24

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