EA200501774A1 - Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции - Google Patents

Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции

Info

Publication number
EA200501774A1
EA200501774A1 EA200501774A EA200501774A EA200501774A1 EA 200501774 A1 EA200501774 A1 EA 200501774A1 EA 200501774 A EA200501774 A EA 200501774A EA 200501774 A EA200501774 A EA 200501774A EA 200501774 A1 EA200501774 A1 EA 200501774A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cells
cultures
products
cultivation
increasing
Prior art date
Application number
EA200501774A
Other languages
English (en)
Other versions
EA008670B1 (ru
Inventor
Кристофер Адам Яллоп
Original Assignee
Круселл Холланд Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Круселл Холланд Б.В. filed Critical Круселл Холланд Б.В.
Publication of EA200501774A1 publication Critical patent/EA200501774A1/ru
Publication of EA008670B1 publication Critical patent/EA008670B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Ophthalmology & Optometry (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Toxicology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Reproductive Health (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Изобретение предлагает способы культивирования клеток, происходящих из эмбриональных клеток-ретинобластов, иммортализованных аденовирусными последовательностями E1, предпочтительно клеток PER.C6™, для улучшения выхода продуктов таких клеток. Предлагаются стратегии питания таких клеток и культур при высоких плотностях, дающие высокий выход продуктов, таких как рекомбинантные антитела.
EA200501774A 2003-05-09 2004-05-06 Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции EA008670B1 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP0350155 2003-05-09
EP0350390 2003-09-01
EP0350940 2003-12-04
EP2004050061 2004-01-30
PCT/EP2004/050724 WO2004099396A1 (en) 2003-05-09 2004-05-06 Cultures of e1-immortalized cells and processes for culturing the same to increase product yields therefrom

Publications (2)

Publication Number Publication Date
EA200501774A1 true EA200501774A1 (ru) 2006-04-28
EA008670B1 EA008670B1 (ru) 2007-06-29

Family

ID=33437116

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200501774A EA008670B1 (ru) 2003-05-09 2004-05-06 Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции

Country Status (15)

Country Link
US (4) US7291484B2 (ru)
EP (1) EP1623023B1 (ru)
JP (1) JP4865539B2 (ru)
KR (1) KR101183770B1 (ru)
CN (2) CN100383238C (ru)
AT (1) ATE414144T1 (ru)
AU (1) AU2004236440B2 (ru)
BR (1) BRPI0409895B8 (ru)
CA (1) CA2520891C (ru)
DE (1) DE602004017709D1 (ru)
DK (1) DK1623023T3 (ru)
EA (1) EA008670B1 (ru)
IL (2) IL171680A (ru)
NZ (1) NZ542585A (ru)
WO (1) WO2004099396A1 (ru)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6783980B2 (en) * 1995-06-15 2004-08-31 Crucell Holland B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy
SI0833934T2 (sl) 1995-06-15 2013-04-30 Crucell Holland B.V. Pakirni sistemi za humani rekombinantni adenovirus za uporabo v genski terapiji
US6855544B1 (en) 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
US7604960B2 (en) 1999-04-15 2009-10-20 Crucell Holland B.V. Transient protein expression methods
US8236561B2 (en) 1999-04-15 2012-08-07 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US7297680B2 (en) 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US7192759B1 (en) 1999-11-26 2007-03-20 Crucell Holland B.V. Production of vaccines
US7527961B2 (en) 1999-11-26 2009-05-05 Crucell Holland B.V. Production of vaccines
US7521220B2 (en) 1999-11-26 2009-04-21 Crucell Holland B.V. Production of vaccines
KR100979025B1 (ko) 2001-12-07 2010-08-30 크루셀 홀란드 비.브이. 바이러스, 바이러스 단리물 및 백신의 생산
CN100383238C (zh) 2003-05-09 2008-04-23 克鲁塞尔荷兰公司 E1-永生化的细胞培养物及培养所述细胞以提高从中获取的产物产量的方法
CA2557046A1 (en) 2004-03-05 2005-10-13 John Crowley Process for cell culturing by continuous perfusion and alternating tangential flow
JP4818936B2 (ja) 2005-01-05 2011-11-16 中外製薬株式会社 細胞の培養方法およびその利用
DE102005054628A1 (de) 2005-11-16 2007-05-24 Cevec Pharmaceuticals Gmbh Verfahren zur Herstellung von permanenten humanen Zelllinien
US11992507B2 (en) 2006-01-23 2024-05-28 Abt Holding Company MAPC therapeutics without adjunctive immunosuppressive treatment
EP1870450A1 (en) * 2006-06-21 2007-12-26 DSMIP Assets B.V. Process for the culturing of E1-immortalized HER cells
SI2041259T1 (sl) 2006-07-14 2016-04-29 Dpx Holdings B.V. Izboljšan postopek kultiviranja celic
AU2011211462C1 (en) * 2006-07-14 2015-01-22 Dpx Holdings B.V. Improved process for the culturing of cells
CN101678079B (zh) 2006-11-28 2013-12-25 韩诺生物制约株式会社 修饰的促红细胞生成素多肽及其治疗用途
TWI601823B (zh) 2007-04-26 2017-10-11 中外製藥股份有限公司 Cell culture methods using media containing high concentrations of amino acids
WO2009023562A2 (en) * 2007-08-09 2009-02-19 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
RU2539786C2 (ru) 2008-09-02 2015-01-27 Плуристем Лтд. Выращенные в прикрепленной к субстрату культуре клетки из ткани плаценты и их использование при лечении
AU2010244592B2 (en) 2009-04-23 2016-05-19 Janssen Vaccines & Prevention B.V. Recombinant human alpha1-antitrypsin
AP3140A (en) * 2009-07-16 2015-02-28 Crucell Holland Bv Production of polio virus at high titers for vaccine production
CN102575233B (zh) 2009-10-15 2014-07-16 克鲁塞尔荷兰公司 从高细胞密度培养物纯化腺病毒的方法
EA028623B1 (ru) 2009-10-15 2017-12-29 Янссен Вэксинс Энд Превеншн Б.В. Способ очистки аденовирусных частиц
WO2012030512A1 (en) 2010-09-03 2012-03-08 Percivia Llc. Flow-through protein purification process
CN107190034A (zh) 2010-10-05 2017-09-22 诺沃—诺迪斯克保健股份有限公司 生产蛋白质的方法
PL2702164T3 (pl) 2011-04-29 2016-06-30 Biocon Res Limited Sposób obniżania heterogeniczności przeciwciał i sposób ich wytwarzania
CN102994547B (zh) * 2011-09-08 2015-05-13 哈药集团技术中心 重组人促红素-ctp融合蛋白生产工艺及应用
WO2014054744A1 (ja) * 2012-10-03 2014-04-10 協和発酵キリン株式会社 培養液にアミノ酸を添加することによるポリペプチドの還元防止方法
KR102245547B1 (ko) 2013-09-30 2021-04-28 얀센 백신스 앤드 프리벤션 비.브이. 고밀도의 미정제 세포 배양 수거물의 정화를 위한 방법
AU2015294014B2 (en) 2014-07-24 2018-08-23 Janssen Vaccines & Prevention B.V. Process for the purification of poliovirus from cell cultures
RU2672318C1 (ru) * 2017-09-19 2018-11-13 Общество с ограниченной ответственностью "Международный Биотехнологический Центр "Генериум" Способ получения моноклональных антител терапевтического назначения с помощью непрерывного культивирования клеток сно
CN113924355B (zh) 2019-05-28 2024-04-02 上海药明生物技术有限公司 用于监测和自动控制灌流细胞培养的拉曼光谱集成灌流细胞培养系统
CN115369069B (zh) * 2022-08-22 2023-12-19 上海健士拜生物科技有限公司 293细胞补料培养基及其制备和应用

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4703008A (en) 1983-12-13 1987-10-27 Kiren-Amgen, Inc. DNA sequences encoding erythropoietin
NZ210501A (en) 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
FR2573436B1 (fr) 1984-11-20 1989-02-17 Pasteur Institut Adn recombinant comportant une sequence nucleotidique codant pour un polypeptide determine sous le controle d'un promoteur d'adenovirus, vecteurs contenant cet adn recombinant, cellules eucaryotes transformees par cet adn recombinant, produits d'excretion de ces cellules transformees et leurs applications, notamment a la constitution de vaccins
IL77081A (en) 1984-12-04 1999-10-28 Genetics Inst Dna sequence encoding human erythropoietin process for the preparation thereof and a pharmaceutical composition of human erythropoietin
US5681718A (en) 1986-03-14 1997-10-28 Celltech Limited Methods for enhanced production of tissue plasminogen activator in cell culture using alkanoic acids or salts thereof
US4835260A (en) 1987-03-20 1989-05-30 Genetics Institute, Inc. Erythropoietin composition
US5192539A (en) 1988-07-21 1993-03-09 Akzo N.V. Infectious bursal disease virus production in continuous cell lines
US5047335A (en) 1988-12-21 1991-09-10 The Regents Of The University Of Calif. Process for controlling intracellular glycosylation of proteins
GB8900483D0 (en) 1989-01-10 1989-03-08 Celltech Ltd Recombinant dna method
DE3923963A1 (de) 1989-07-20 1991-01-31 Behringwerke Ag Muteine des menschlichen erythropoetins, ihre herstellung und ihre verwendung
US5856298A (en) 1989-10-13 1999-01-05 Amgen Inc. Erythropoietin isoforms
ATE150486T1 (de) 1991-08-01 1997-04-15 Fond Nat Transfusion Sanguine Expression in nicht-tumorbildenden menschlichen lymphoblasten zellinien mit einem integrierenden vektor
US5384249A (en) 1991-12-17 1995-01-24 Kyowa Hakko Kogyo Co., Ltd. α2→3 sialyltransferase
CN1057534C (zh) 1993-08-17 2000-10-18 柯瑞英-艾格公司 促红细胞生成素类似物
US5733782A (en) 1993-10-25 1998-03-31 Creative Biomolecules, Inc. Methods and compositions for high protein production from non-native DNA
US5773569A (en) 1993-11-19 1998-06-30 Affymax Technologies N.V. Compounds and peptides that bind to the erythropoietin receptor
US5830851A (en) 1993-11-19 1998-11-03 Affymax Technologies N.V. Methods of administering peptides that bind to the erythropoietin receptor
US5789247A (en) 1994-04-01 1998-08-04 Ballay; Annick Expression in non-tumoral human lymphoblastoid lines with an integrative vector
US5610043A (en) 1994-04-28 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services Human prostatic cell lines immortalized by adenovirus 12-simian virus 40 (AD12/SV40) hybrid virus
US5767078A (en) 1995-06-07 1998-06-16 Johnson; Dana L. Agonist peptide dimers
US6265212B1 (en) 1995-06-15 2001-07-24 Introgene B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy
SI0833934T2 (sl) 1995-06-15 2013-04-30 Crucell Holland B.V. Pakirni sistemi za humani rekombinantni adenovirus za uporabo v genski terapiji
US5989805A (en) 1995-10-27 1999-11-23 Board Of Trustees Operating Michigan State University Immortal avian cell line to grow avian and animal viruses to produce vaccines
EA004928B1 (ru) 1995-11-13 2004-10-28 Такара Сузо Ко., Лтд Полипептиды, повышающие эффективность генного переноса в клетки-мишени с помощью ретровируса, и гены, их кодирующие
US5681597A (en) * 1996-02-06 1997-10-28 Liquid Container L.P. Vacuum conveyor picker for blow bottle container
US5856292A (en) 1996-04-08 1999-01-05 Colgate Palmolive Company Light duty liquid cleaning compositions
US5835382A (en) 1996-04-26 1998-11-10 The Scripps Research Institute Small molecule mimetics of erythropoietin
BR9712675A (pt) 1996-10-25 1999-10-19 Searle & Co Agonistas do receptor de eritropoietina circularmente permutados.
US7732129B1 (en) * 1998-12-01 2010-06-08 Crucell Holland B.V. Method for the production and purification of adenoviral vectors
CA2283253A1 (en) 1997-03-04 1998-09-11 Baxter International Inc. Adenovirus e1-complementing cell lines
WO1999066041A1 (en) 1998-06-16 1999-12-23 Human Genome Sciences, Inc. 94 human secreted proteins
ATE290091T1 (de) 1997-03-27 2005-03-15 Univ British Columbia Insekten-expressionsvektoren
CA2221819A1 (en) 1997-03-27 1998-09-27 Thomas A. Gigliatti Insect expression vectors
US5997128A (en) * 1997-05-30 1999-12-07 Hewlett-Packard Company Translational service station for imaging inkjet printheads
EP0986644B1 (de) 1997-07-23 2006-10-04 Boehringer Mannheim GmbH Herstellung von erythropoietin durch endogene genaktivierung mit viralen promotoren
ES2523599T3 (es) 1997-12-03 2014-11-27 Roche Diagnostics Gmbh Eritropoyetina de gran actividad específica
US6670188B1 (en) 1998-04-24 2003-12-30 Crucell Holland B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy
AU784550B2 (en) 1999-04-13 2006-05-04 Kenneth S. Warren Institute, Inc., The Modulation of excitable tissue function by peripherally administered erythropoietin
US7297680B2 (en) * 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US20050164386A1 (en) * 1999-04-15 2005-07-28 Uytdehaag Alphonsus G. Overexpression of enzymes involved in post-translational protein modifications in human cells
CN100457914C (zh) * 1999-04-15 2009-02-04 荷兰克鲁塞尔公司 用编码腺病毒e1蛋白的序列在人体细胞中生产重组蛋白
WO2003048197A1 (en) 2001-12-07 2003-06-12 Crucell Holland B.V. Production and of viruses, viral isolates and vaccines
US6855544B1 (en) 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
IL145893A0 (en) 1999-04-26 2002-07-25 Genentech Inc Cell culture process for glycoproteins
US6215499B1 (en) * 1999-05-06 2001-04-10 Phillips Petroleum Company Method and apparatus for interactive curved surface seismic interpretation and visualization
US6492169B1 (en) 1999-05-18 2002-12-10 Crucell Holland, B.V. Complementing cell lines
US6558948B1 (en) 1999-11-23 2003-05-06 Stefan Kochanek Permanent amniocytic cell line, its production and use for the production of gene transfer vectors
EP1103610A1 (en) * 1999-11-26 2001-05-30 Introgene B.V. Production of vaccines from immortalised mammalian cell lines
EP1184458A1 (en) 2000-08-28 2002-03-06 U-BISys B.V. Differentially expressed CD46 epitopes, proteinaceous molecules capable of binding thereto, and uses thereof
PA8536201A1 (es) 2000-12-29 2002-08-29 Kenneth S Warren Inst Inc Protección y mejoramiento de células, tejidos y órganos que responden a la eritropoyetina
AU2002316230A1 (en) * 2001-06-13 2002-12-23 Genentech, Inc. Methods of culturing animal cells and polypeptide production in animal cells
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
EP2390331B1 (en) * 2001-09-28 2018-04-25 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. MicroRNA molecules
KR100979025B1 (ko) 2001-12-07 2010-08-30 크루셀 홀란드 비.브이. 바이러스, 바이러스 단리물 및 백신의 생산
WO2003051927A2 (en) 2001-12-17 2003-06-26 Crucell Holland B.V. Production of f(ab')2 fragments in mammalian cells
WO2003093441A2 (en) * 2002-05-03 2003-11-13 Duke University A method of regulating gene expression
CA2491567A1 (en) 2002-07-01 2004-01-08 The Kenneth S. Warren Institute, Inc. Recombinant tissue protective cytokines and encoding nucleic acids thereof for protection, restoration, and enhancement of responsive cells, tissues, and organs
US20040268441A1 (en) * 2002-07-19 2004-12-30 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
US8524477B2 (en) 2002-10-29 2013-09-03 Crucell Holland B.V. Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus E1A protein, and proteins obtained thereby
DE60335024D1 (de) * 2002-12-23 2010-12-30 Bristol Myers Squibb Co Produktqualitätsverbesserung in säugerzellkulturverfahrenzur proteinproduktion
CN100383238C (zh) 2003-05-09 2008-04-23 克鲁塞尔荷兰公司 E1-永生化的细胞培养物及培养所述细胞以提高从中获取的产物产量的方法
US20050144669A1 (en) * 2003-07-01 2005-06-30 Whitehead Institute For Biomedical Research MicroRNAs in plants
US20050120415A1 (en) * 2003-10-09 2005-06-02 E.I. Du Pont De Nemours And Company Gene silencing
US7642078B2 (en) 2005-12-28 2010-01-05 Crucell Holland B.V. Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus E1A protein, and proteins obtained thereby
EP2081589B1 (en) 2006-11-16 2010-10-06 Crucell Holland B.V. Complementation of factor xi deficiency by factor v mutants

Also Published As

Publication number Publication date
AU2004236440B2 (en) 2008-10-09
US20060240513A1 (en) 2006-10-26
EP1623023A1 (en) 2006-02-08
CN1784489A (zh) 2006-06-07
BRPI0409895B8 (pt) 2021-05-25
EP1623023B1 (en) 2008-11-12
US20070031932A1 (en) 2007-02-08
EA008670B1 (ru) 2007-06-29
BRPI0409895A (pt) 2006-05-23
CN101328473A (zh) 2008-12-24
US7608431B2 (en) 2009-10-27
US8008043B2 (en) 2011-08-30
ATE414144T1 (de) 2008-11-15
CA2520891A1 (en) 2004-11-18
BRPI0409895B1 (pt) 2018-09-18
IL171680A (en) 2010-05-31
CA2520891C (en) 2014-07-08
CN101328473B (zh) 2012-05-02
CN100383238C (zh) 2008-04-23
NZ542585A (en) 2007-11-30
WO2004099396A1 (en) 2004-11-18
US7291484B2 (en) 2007-11-06
DE602004017709D1 (de) 2008-12-24
DK1623023T3 (da) 2009-03-02
US20060121611A1 (en) 2006-06-08
KR101183770B1 (ko) 2012-09-17
JP2006525800A (ja) 2006-11-16
JP4865539B2 (ja) 2012-02-01
IL198861A (en) 2011-12-29
HK1080115A1 (en) 2006-04-21
KR20060003081A (ko) 2006-01-09
AU2004236440A1 (en) 2004-11-18
US20100311160A1 (en) 2010-12-09

Similar Documents

Publication Publication Date Title
EA200501774A1 (ru) Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции
ATE488600T1 (de) Produktqualitätsverbesserung in säugerzellkulturverfahrenzur proteinproduktion
EA201490631A1 (ru) Способы культивирования клеток млекопитающих для получения белка
MX2007005132A (es) Medios libres de proteina animal para cultivo de celulas.
MA39867A (fr) Procédés d'isolement, de culture et de manipulation génétique de populations de cellules immunitaires pour une thérapie adoptive
MX2009011363A (es) Metodo de produccion de proteinas utilizando compuestos anti-senectud.
TW200512297A (en) Mammalian cell culture processes for protein production
EA201171018A1 (ru) Биореактор для культивирования клеток млекопитающих
NO20081622L (no) Fremgangsmater for produksjon av proteiner ved anvendelse av anti-senescence forbindelser
NO20072719L (no) Serumfritt cellekulturmedium for pattedyrceller
WO2007016485A3 (en) Use of a gsk-3 inhibitor to maintain potency of cultured cells
BRPI0516572A (pt) métodos e composições para melhorar a produção de proteìnas recombinantes
WO2011091350A3 (en) Methods & compositions for improving protein production
NZ597334A (en) Cell culture improvements
RU2009143635A (ru) Улучшение титра полипептида фактора viii в клеточных культурах
WO2002101019A3 (en) Methods of culturing animal cells and polypeptide production in animal cells
EP2588594A4 (en) PROCESS FOR CULTIVATING UNDIFFERENCED CELLS USING DELAYED RELATED COMPOSITION COMPOSITIONS
MX2009007354A (es) Proceso para producir biomasa y proteinas mediante microalgas.
NO20064379L (no) Anvendelse av et serumfritt cellekulturmedium for produsjonen av IL-18BP i pattedyrceller
EA201170025A1 (ru) Способы повышения жизнеспособности и продукции культуры клеток
CA2796599A1 (en) Method for producing induced pluripotent stem cells
NZ599479A (en) Improved reverse genetics methods for virus rescue
EA201000377A1 (ru) Способ получения фибробластоподобных клеток из пупочного канатика новорожденного
WO2007056062A3 (en) Methods for adapting mammalian cells
EA200701000A1 (ru) Культуральная среда для клеток млекопитающих, не содержащая сыворотки

Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ KZ KG MD TJ TM