ECSP17025497A - Marcadores de polimorfismo unico de nucleotide (snp) para stevia - Google Patents

Marcadores de polimorfismo unico de nucleotide (snp) para stevia

Info

Publication number
ECSP17025497A
ECSP17025497A ECIEPI201725497A ECPI201725497A ECSP17025497A EC SP17025497 A ECSP17025497 A EC SP17025497A EC IEPI201725497 A ECIEPI201725497 A EC IEPI201725497A EC PI201725497 A ECPI201725497 A EC PI201725497A EC SP17025497 A ECSP17025497 A EC SP17025497A
Authority
EC
Ecuador
Prior art keywords
stevia
markers
snp
rebm
rebd
Prior art date
Application number
ECIEPI201725497A
Other languages
English (en)
Inventor
Seong Siang Ong
jian ning Chen
Avetik Markosyan
Yu Cheng Bu
Yeen Yee Wong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201510037435.8A external-priority patent/CN105850727B/zh
Priority claimed from CN201510036580.4A external-priority patent/CN105850709B/zh
Priority claimed from CN201510036668.6A external-priority patent/CN105850750B/zh
Application filed filed Critical
Publication of ECSP17025497A publication Critical patent/ECSP17025497A/es

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12Leaves
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • A23L27/33Artificial sweetening agents containing sugars or derivatives
    • A23L27/36Terpene glycosides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04Plant cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Se divulgan variedades de estevia con un alto contenido de RebD, un alto contenido de RebM y un alto contenido de RebD y RebM, con varios marcadores SNP e isoformas UGT. Igualmente se divulgan métodos para filtrar los SNPs y para utilizarlos en reproducción mediante el uso de marcadores. Asimismo, se describen los métodos para la introgresión de los SNPs divulgados, relacionados con alto RebD y alto RebM en plantas de estevia, seleccionando plantas que contengan uno o más SNPs y cruzándolas para obtener los fenotipos agronómicos deseados en la progenie de las plantas.
ECIEPI201725497A 2014-09-26 2017-04-25 Marcadores de polimorfismo unico de nucleotide (snp) para stevia ECSP17025497A (es)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201462071567P 2014-09-26 2014-09-26
US201462071568P 2014-09-26 2014-09-26
US201462071566P 2014-09-26 2014-09-26
US201462064601P 2014-10-16 2014-10-16
CN201510037435.8A CN105850727B (zh) 2015-01-23 2015-01-23 甜叶菊新品种及高rd、rm含量甜菊糖苷的制备
CN201510036580.4A CN105850709B (zh) 2015-01-23 2015-01-23 甜叶菊新品种817096谱星5号及高rd含量甜菊糖苷的制备
CN201510036668.6A CN105850750B (zh) 2015-01-23 2015-01-23 甜叶菊新品种814011谱星3号及高rm含量甜菊糖苷的制备
US201562116893P 2015-02-16 2015-02-16

Publications (1)

Publication Number Publication Date
ECSP17025497A true ECSP17025497A (es) 2018-05-31

Family

ID=55582096

Family Applications (1)

Application Number Title Priority Date Filing Date
ECIEPI201725497A ECSP17025497A (es) 2014-09-26 2017-04-25 Marcadores de polimorfismo unico de nucleotide (snp) para stevia

Country Status (5)

Country Link
US (3) US10370673B2 (es)
EP (1) EP3197269A1 (es)
CN (2) CN107205353B (es)
EC (1) ECSP17025497A (es)
WO (1) WO2016049531A1 (es)

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AR117508A1 (es) * 2018-12-28 2021-08-11 Suntory Holdings Ltd Planta de stevia con bajo contenido de tiramina
PE20212081A1 (es) * 2019-04-11 2021-10-28 Suntory Holdings Ltd Planta de stevia con baja formacion de polen
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BR112022022853A2 (pt) * 2020-05-12 2022-12-20 Suntory Holdings Ltd Planta de estévia, semente, tecido, folha seca, cultura de tecidos ou célula, métodos para produzir uma planta de estévia, um extrato, rebaudiosídeo d, rebaudiosídeo m, rebaudiosídeo n e alimento ou bebida, extrato da planta, método de triagem para a planta de estévia, e, kit de triagem
PY2137798A (es) * 2020-05-12 2022-02-17 Suntory Holdings Ltd Planta de stevia con alto contenido de rebaudiósido e
CN115552039A (zh) * 2020-05-12 2022-12-30 三得利控股株式会社 瑞鲍迪苷m的含有比高的甜菊植物及其筛选方法
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CN111793660A (zh) * 2020-09-02 2020-10-20 安徽大学 一种具有降血糖作用的黄大茶多糖
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