ECSP910731A - BACTERIAL VECTORS - Google Patents
BACTERIAL VECTORSInfo
- Publication number
- ECSP910731A ECSP910731A ECSP910731A ECSP910731A EC SP910731 A ECSP910731 A EC SP910731A EC SP910731 A ECSP910731 A EC SP910731A EC SP910731 A ECSP910731 A EC SP910731A
- Authority
- EC
- Ecuador
- Prior art keywords
- spec
- novel
- lactococous
- production
- msp
- Prior art date
Links
- 239000013598 vector Substances 0.000 title abstract 2
- 230000001580 bacterial effect Effects 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 239000000047 product Substances 0.000 abstract 2
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
- 102100032467 Transmembrane protease serine 13 Human genes 0.000 abstract 1
- 239000012228 culture supernatant Substances 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 101150103899 msp gene Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 239000013605 shuttle vector Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La invención se refiere a ingeniería genética y provee novedosas moléculas de ADN que comprenden orígenes multifuncionales de replicación y/o una secuencia de ADN que codifica para la proteína más abundante en el sobrenadante de cultivos de Lactococous spec., denominado Producto de Soreción Mayor (MSP), para el péptido de señal y/o el promotor del gen de MSP. Las novedosas moléculas de ADN son usadas para la producción de novedosos vectores en lanzadera para clonar ADN en al menos E.coli y Lactococous spec., o novedosos vectores para la expresión de genes homólogos o heterólogos y la producción de productos de genes segregados en bacterias gram-positivas como Lactococous spec. y Bacillus Spec.The invention relates to genetic engineering and provides novel DNA molecules comprising multifunctional origins of replication and / or a DNA sequence encoding the most abundant protein in the Lactococous spec. Culture supernatant, called the Major Sorption Product (MSP ), for the signal peptide and / or the promoter of the MSP gene. The novel DNA molecules are used for the production of novel shuttle vectors to clone DNA into at least E.coli and Lactococous spec., Or novel vectors for the expression of homologous or heterologous genes and the production of secreted gene products in bacteria. gram-positive like Lactococous spec. and Bacillus Spec.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ECSP910731 ECSP910731A (en) | 1991-03-15 | 1991-03-15 | BACTERIAL VECTORS |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ECSP910731 ECSP910731A (en) | 1991-03-15 | 1991-03-15 | BACTERIAL VECTORS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ECSP910731A true ECSP910731A (en) | 1992-02-14 |
Family
ID=42045520
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ECSP910731 ECSP910731A (en) | 1991-03-15 | 1991-03-15 | BACTERIAL VECTORS |
Country Status (1)
| Country | Link |
|---|---|
| EC (1) | ECSP910731A (en) |
-
1991
- 1991-03-15 EC ECSP910731 patent/ECSP910731A/en unknown
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