ECSP982590A - POLLEN-BASED TRANSFORMATION SYSTEM USING SOLID MEDIA - Google Patents
POLLEN-BASED TRANSFORMATION SYSTEM USING SOLID MEDIAInfo
- Publication number
- ECSP982590A ECSP982590A ECSP982590A ECSP982590A EC SP982590 A ECSP982590 A EC SP982590A EC SP982590 A ECSP982590 A EC SP982590A EC SP982590 A ECSP982590 A EC SP982590A
- Authority
- EC
- Ecuador
- Prior art keywords
- pollen
- plant
- agrobacteria
- medium
- applying
- Prior art date
Links
- 239000007787 solid Substances 0.000 title abstract 3
- 230000009466 transformation Effects 0.000 title abstract 3
- 239000002609 medium Substances 0.000 abstract 3
- 230000009261 transgenic effect Effects 0.000 abstract 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 229920000936 Agarose Polymers 0.000 abstract 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 1
- 229930006000 Sucrose Natural products 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 230000035784 germination Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 1
- 235000019341 magnesium sulphate Nutrition 0.000 abstract 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 abstract 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 239000005720 sucrose Substances 0.000 abstract 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Esta invención describe un medio que soporta la germinación de polen y el crecimiento de tubos polínicos en presencia de Agrobacteria, el mismo que comprende agarosa, sucorosa, NO3, MnSO4, H3B03, MgSO4 y ácido hiberélico. Se describe un método para la transformación genética de plantas y lineas por medio de una transformación basada en polen, regulada con Agrobacteria. El método comprende los pasos para la obtención de polen de una primera planta, la aplicación de una capa de agrobacterias a un medio sólido de cultivos de polen. La Agrobacteria contiene por lo menos una secuencia heterogénea de genes capaz de ser transferida a una célula vegetal, aplicando al polen al medio sólido y permitiendo que el polen germine y crezca en el medio, por lo que la Agrobacteria se adhiere al tubo polínico o regula la transferencia de la secuencia heterogénea de genes de polen que está germinando para obtener el polen transgénico, aplicando este último al estigma de una segunda planta que puede ser fertilizada por el polen de la primera planta, fertilizando asi la segunda planta y germinando la semilla transgénica para obtener una planta transgénica. El método es especialmente adecuado para el uso con el llamado polen de la estigma seco.This invention describes a medium that supports the germination of pollen and the growth of pollen tubes in the presence of Agrobacteria, which comprises agarose, sucrose, NO3, MnSO4, H3B03, MgSO4 and hibernic acid. A method for the genetic transformation of plants and lines is described by means of a pollen-based transformation, regulated with Agrobacteria. The method comprises the steps for obtaining pollen from a first plant, applying a layer of agrobacteria to a solid pollen culture medium. Agrobacteria contains at least one heterogeneous gene sequence capable of being transferred to a plant cell, applying pollen to the solid medium and allowing pollen to germinate and grow in the medium, so that Agrobacteria adhere to the pollen tube or regulate the transfer of the heterogeneous pollen gene sequence that is germinating to obtain the transgenic pollen, applying the latter to the stigma of a second plant that can be fertilized by the pollen of the first plant, thus fertilizing the second plant and germinating the transgenic seed to obtain a transgenic plant. The method is especially suitable for use with so-called dry stigma pollen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ECSP982590 ECSP982590A (en) | 1998-07-14 | 1998-07-14 | POLLEN-BASED TRANSFORMATION SYSTEM USING SOLID MEDIA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ECSP982590 ECSP982590A (en) | 1998-07-14 | 1998-07-14 | POLLEN-BASED TRANSFORMATION SYSTEM USING SOLID MEDIA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ECSP982590A true ECSP982590A (en) | 1999-03-02 |
Family
ID=42043510
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ECSP982590 ECSP982590A (en) | 1998-07-14 | 1998-07-14 | POLLEN-BASED TRANSFORMATION SYSTEM USING SOLID MEDIA |
Country Status (1)
| Country | Link |
|---|---|
| EC (1) | ECSP982590A (en) |
-
1998
- 1998-07-14 EC ECSP982590 patent/ECSP982590A/en unknown
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