EP0047149A1 - Une polypeptide purifiée, un procédé de sa préparation, son utilisation et compositions pharmaceutiques la contenant - Google Patents

Une polypeptide purifiée, un procédé de sa préparation, son utilisation et compositions pharmaceutiques la contenant Download PDF

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Publication number
EP0047149A1
EP0047149A1 EP81303944A EP81303944A EP0047149A1 EP 0047149 A1 EP0047149 A1 EP 0047149A1 EP 81303944 A EP81303944 A EP 81303944A EP 81303944 A EP81303944 A EP 81303944A EP 0047149 A1 EP0047149 A1 EP 0047149A1
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EP
European Patent Office
Prior art keywords
grpp
water
purified polypeptide
acid
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
EP81303944A
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German (de)
English (en)
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EP0047149B1 (fr
Inventor
Alister James Moody
Lars Thim
Karin Damm Jorgensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Novo Industri AS
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Priority to AT81303944T priority Critical patent/ATE6639T1/de
Publication of EP0047149A1 publication Critical patent/EP0047149A1/fr
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Publication of EP0047149B1 publication Critical patent/EP0047149B1/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Definitions

  • the present invention relates to a purified peptide (herein designated GRPP) and salts thereof, to a process for preparing GRPP and salts thereof, to the use of GRPP and physiologically acceptable salts thereof as a medicament and to pharmaceutical compositions containing GRPP or physiologically acceptable salts thereof.
  • GRPP purified peptide
  • GRPP and salts thereof show interesting pharmacological properties, for example an inhibition of the gastric acid secretion.
  • GRPP is a peptide having the following amino acid sequence:
  • the molecular weight of GRPP as calculated from the amino acid content is 3445 and the isoelectric point of GRPP is 4.0 + 0.2.
  • GRPP may be extracted from porcine pancreas glands.
  • GRPP is isolated from the mother liquor from the .so-called insulin salt cake obtained in the production of insulin.
  • the insulin salt cake may be prepared as follows:
  • the mother liquor from the insulin salt cake may be used as starting material for the isolation of GRPP.
  • the insulin salt cake, and consequently the mother liquor may be prepared in many other ways, vide for example Ind.Eng.Chem. 32 (1940), 908 - 910. Different methods are used by different insulin manufacturers, however, in all cases GRPP will probably be present in the mother liquor from the insulin salt cake.
  • the isolation and purification of GRPP from the above mother liquor is performed by the use of chromatography, for example anion and cation exchange chromatography, and gel filtration, and said operations are performed in a manner known per se.
  • the first step in the isolation may be a desalting process, for example ultrafiltration or reverse osmosis.
  • a desalting process for example ultrafiltration or reverse osmosis.
  • an extensive dilution of the mother liquor with water for example between about 10 and 50 times, may be performed.
  • the chromatography is preferably carried out in an aqueous medium, however, water-miscible solvents such as lower alkanols may be added.
  • cation exchange chromatography In order to carry out cation exchange chromatography, it is desirable to use an eluent having a pH in the range of from about 2 to 6.
  • cation exchangers carboxymethylcellulose; carboxymethyl-Sephadex and SP Sephadex may be mentioned.
  • anion exchange chromatography In order to carry out anion exchange chromatography, it is desired to use an eluent having a pH in the range from about 4 to 9.
  • anion exchangers DEAE cellulose, DEAE Sephadex and QAE Sephadex may be mentioned.
  • the ion exchange chromatography is carried out by the collection of the fractions containing the majority of G RPP .
  • the anion and cation exchange chromatography are carried out in any order. If more than one cation or anion chromatography is carried out the two operations are preferably performed with eluents having different pH-values.
  • the gel filtration is preferably carried out in volatile buffers such as weak acetic acid, ammonium bicarbonate or ammonium formate.
  • volatile buffers such as weak acetic acid, ammonium bicarbonate or ammonium formate.
  • the final product may be recovered by lyophilization.
  • GRPP may be isolated directly from the aqueous, acidic extract of porcine pancreas glands containing a water-miscible, organic solvent by the use of ion exchange chromatography in analogy with the above processes.
  • salts of GRPP are salts with cations such as sodium, potassium, magnesium, calcium and zinc, and acid addition salts with organic or inorganic acids such as formic, methansulfonic, hydrochloric and sulphuric acid.
  • Physiologically acceptable salts of GRPP are preferred.
  • the salts may be prepared from GRPP and the cation or acid in question in any suitable manner. Such methods are known to the skilled art worker.
  • GRPP inhibits pentagastrin stimulated gastric acid secretion in rats and in cats in vivo.
  • 5 pg/kg pentagastrin plus 16 ⁇ g/kg glucagon gave an increase of 185 ⁇ Eq acid.
  • GRPP can be used to control the gastric acid secretion in mammals, for example in man. This means that GRPP may be used in the treatment of gastroduodenal ulcer diseases in man.
  • the dosage rates of GRPP and salts thereof can be adjusted according to the magnitude of desired response and with other factors generally taken into consideration in establishing prescription of a particular dosage.
  • a dosage range from 1 to 500 pg, preferably from 10 to 50 p g, per kg body weight is suitable, although a lower or higher dosage may be administered safely.
  • GRPP and salts thereof are converted into pharmaceutical preparations and administered, preferably to humans, in analogy with known methods.
  • GRPP and salts thereof may be administered intraveneously, intramuscularly or subcutaneously. Furthermore, GRPP and salts thereof may be administered by the nasal or rectal route. GRPP and salts thereof may possibly be administered orally.
  • GRPP or salts thereof may be dissolved in distilled water and the pH is adjusted to about 6 to 8.
  • lactose may be added to the solution.
  • the solution is sterile filtered and filled in vials. Thereafter, the solutions are lyophilized and the vials are sealed under aseptic conditions.
  • a solution in a nasal spraying device or nebulisator may be used.
  • GRPP or a salt thereof is dissolved in distilled water, the pH is adjusted to about 6 to 8 by adding, for example, sodium phosphate and citric acid as buffer.
  • Sodium chloride, sorbitol and glycerol may be used to obtain an isotonic solution with a suitable viscosity.
  • the solution is administered by the use of a suitable nebulisator or plastic spray.
  • the solution may be preserved by the use of known preservatives and a known surfactant may be added.
  • GRPP may be mixed with suitable constituents and a mixture of volatile halogencarbons, for example monofluorotrichloromethane, difluorodichloromethane and tetrafluorodichloroethane in order to obtain a mixture with a vapour pressure producing a well defined single dose when the mixture is administered by the use of a dose aerosol spray.
  • suitable constituents for example monofluorotrichloromethane, difluorodichloromethane and tetrafluorodichloroethane
  • suppositories are produced by admixing GRPP or a salt thereof with an inactive constituent such as cocoa butter or with a base such as Polysorbate 85, propylene glycol monostearate and white bee's wax.
  • the present invention also relates to a pharmaceutical composition
  • a pharmaceutical composition comprising purified GRPP or a salt thereof in pharmaceutically acceptable carrier(s), diluent(s) or exhi- pient(s) thereto.
  • aqueous sodium chloride can be mentioned.
  • a convenient preparation of GRPP may be a sterile aqueous solution of GRPP or a salt thereof containing about 0.9% sodium chloride and optionally an effective amount of a preservative, such as methyl or propyl p-hydroxybenzoate or phenol.
  • the aqueous solution may contain, for example from 10 to 100 p g of GRPP or a salt thereof per ml.
  • a starting material for preparing GRPP preparations which has a purity of at least 50%, preferably a purity of at least 90% of GRPP or a salt thereof.
  • the distribution of GRPP during fractionation was measured with the following radio immuno assay.
  • Antiglicentin serum R-64 (which reacts with glicentin 12-30 ) was used in the radio immuno assay.
  • the immuno assay consisted of incubating 100 pl of standards of porcine glicentin, or of unknowns, with equal volumes of antiserum diluted (1:65,000) in a 1:200 dilution of non-immune rabbit serum for 72 hours.
  • (125-I)glicentin 100 ⁇ l of a solution containing 60 pg (125-I)glicentin/ml was then added and the incubation continued for 24 hours.
  • the free and antibody bound (125-I)glicentin was separated by centrifugation 24 hours after the addition of 100 ⁇ l anti-rabbit IgG serum (DAKO, Copenhagen). Under these conditions R-64 did not react significantly with extracts of rat or human pancreas or with VIP, glucagon and GIP.
  • step a To the mother liquor from step a) (162 litres) 34 kg of solid ammonium sulphate was added. After ' 2 hours' stirring at 25°C, the precipitate (223 g wet weight) was collected by centrifugation.
  • the sucrose gradient was prepared with a "LKB” gradient mixer (No. 8122).
  • the GRPP-peak was pooled and lyophilized.
  • the peptide isolated by the above method represents a crude non-homogeneous preparation of GRPP.
  • a final purification of GRPP was obtained in point f).
  • the electrophoresis buffer was 10% (v/v) pyridine/ 0.3% (v/v) acetic acid (pH 6.5).
  • the solvent in the ascending chromatography in the second dimension was n-butanol/acetic acid/water/pyridine (15:3:12:10 by vol.).
  • GRPP was localized by spraying with a solution of 0.1% ninhydrin/ 3% 2,4,6-collidine/10% acetic acid in ethanol.
  • the cellulose layer corresponding to GRPP (R f value in chromatography: 0.43) was scraped off and GRPP was extracted with 2 x 300 pl of 50% acetic acid, and lyophilized.
  • the product obtained represented highly purified GRPP.
  • the words "Avicel” and "Shandon" are Trade Marks.
  • Example 1 The lyophilized material from step d) in Example 1 was dissolved in 1 ml of water and 0.5 ml thereof was added to 0.25 ml of 0.058 M TRIS/0.05 M hydrochloric acid/0.18 M sodium chloride buffer (pH 7.4). The mixture was applied to a "QAE-Sephadex A-25" column (0.7 x 9 cm) which was equilibrated with the buffer added. The column was eluted with equilibrating buffer, whereby GRPP eluted in 5.5 column volumes.
  • the purity of the product was approximately equal to the purity of the product obtained from the "QAE column"described in Example 1 e).
  • Example 1 e The product was desalted on a "Bio-gel P-30" column, vide Example 1 e), and may be further purified, vide the process of Example 1 f).
  • Example 1 100 litres of mother liquor corresponding to 140 kg of porcine pancreas, vide step a) of Example 1, was concentrated to 45 litres by ultrafiltration (DDS-800 membrane, supplied from De Danske Sukkerfabrikker A/S, Copenhagen, Denmark). 45 litres of water was added and the 0047149 tration was continued until a volume of 2.6 litre was reached. This volume was kept constant by the addition of water and the ultrafiltration was continued until the conductivity of the concentrate was 3.3 mS.
  • DDS-800 membrane supplied from De Danske Sukkerfabrikker A/S, Copenhagen, Denmark
  • the pH of the concentrate was adjusted to 3.15 with 15 ml of formic acid and 0.4 litres of the following eluant was added: 100 mM formic acid/20 mM sodium hydroxide buffer (pH 3.15).
  • the sample was applied to a "SP-Sephadex C-25" column (10 x 80 cm) equilibrated with the eluant at a flow rate of 600 ml/hour.
  • the column was eluted with 10 litres of the eluant and then with a linear gradient of sodium chloride (0 - 0.4 M) in the eluant.
  • the total volume of the gradient was 60 litres and fractions of 870 ml were collected.
  • GRPP was eluted with the gradient at a molarity of sodium chloride of about 0.28 M. Fractions Nos. 47 - 52 were pooled.
  • the pooled fractions from step b) were dialyzed 4 hours against 25 litres of water and then 18 hours against the following eluant: 57.7 mM TRIS/50 mM hydrochloric acid buffer (pH 7.4). The pH was adjusted to 7.4 with 110 ml of 2 M TRIS and 1 litre of water was added. The solution, which had a conductivity of 4.8 mS, was applied to a "QAE-Sephadex A-25" column (2.5 x 50 cm) equilibrated with the above eluant at a flow rate of 50 ml/hour.
  • the lyophilized powder was dissolved in 100 ml of water and dialyzed 16 hours against water.
  • the turbid solution was filtered and fractionated on a 440 ml "LKB" isoelectric focusing column with a mixture of ampholines (pH 2.5 - 5.0), vide Acta Chem.Scand., supra.
  • the dense, light, anode and cathode solutions were as described in Example 1 d. Fractions of 3.8 ml were collected and fractions Nos. 77 - 87 were pooled.
  • GRPP focused as a peak with an isoelectric pH of 4.0.
  • step e The pooled fractions from step e) were lyophilized and the powder dissolved in 3 ml of 50 mM ammonium hydrogencarbonate (pH 7.8). The solution was gel filtered on a "Bio-gel P-10" column (1.5 x 97 cm) equilibrated with 50 mM ammonium hydrogencarbonate. The flow rate was 5 ml/hour and fractions of 1.67 ml were collected. Fractions Nos. 43 - 48 were pooled and lyophilized.
  • a single porcine pancreas (62 g) was homogenized in 372 ml of 66% ethanol containing 0.36% phosphoric acid. After 1 hour's mixing at room temperature, the extract was centrifuged. The supernatant was collected and the tissue re-extracted with 100 ml of 60% ethanol containing 0.3% phosphoric acid. The pooled supernatants (400 ml) were clarified by passage through "Hi-Flo" (trade mark). GRPP was adsorbed from the extract by adding 1.3 g of dry "SP C-25 Sephadex" to the extract and stirring. After 1 hour a further 1.3 g of "SP C-25 Sephadex" were added.
  • the "SP Sephadex” was removed from the extract by filtration and washed on the filter successively with 30 ml 60% ethanol/0.3% phosphoric acid and with 30 ml 60% ethanol/60 ml water and then sucked dry.
  • the dry "SP Sephadex” was suspended in 2 x 15 ml of ammonium chloride solution (1 mol/I), pH 8.0, and the suspension filtered to collect the GRPP-rich filtrate.
  • the "SP Sephadex” was washed once on the filter with 10 ml of ammonium chloride solution (1 mol/1), pH 8.5, and the filtrates combined.
  • GRPP was precipitated by adding 19 g of ammonium sulphate to the filtrate (38 ml) and centrifuging after 1 hour's standing at room temperature. The yield of GRPP was 49 ug.
  • This material may be further purified, for example, by the process described in Example 1 or 3.
  • a preparation for parenteral administration containing 1 mg of GRPP per ml may be prepared as follows:
  • the preparation in any of the vials is to be dissolved in 1.0. ml of sterile, distilled water. To humans 1 ml of said solution may be administered.
  • a preparation for parenteral administration containing 10 mg of GRPP per ml may be prepared as follows:
  • Rectal suppositories are prepared by admixing 1 mg of GRPP with 4 g of cocoa butter.
  • a solution for administration by a nasal plastic spray may be prepared as follows:

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pyrane Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
EP81303944A 1980-08-28 1981-08-27 Une polypeptide purifiée, un procédé de sa préparation, son utilisation et compositions pharmaceutiques la contenant Expired EP0047149B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT81303944T ATE6639T1 (de) 1980-08-28 1981-08-27 Ein gereinigtes polypeptid, ein verfahren zu dessen herstellung, dessen anwendung und das enthaltende pharmazeutische praeparat.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK366380A DK366380A (da) 1980-08-28 1980-08-28 Anvendelsen af grp og salte deraf
DK3663/80 1980-08-28

Publications (2)

Publication Number Publication Date
EP0047149A1 true EP0047149A1 (fr) 1982-03-10
EP0047149B1 EP0047149B1 (fr) 1984-03-14

Family

ID=8125416

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Application Number Title Priority Date Filing Date
EP81303944A Expired EP0047149B1 (fr) 1980-08-28 1981-08-27 Une polypeptide purifiée, un procédé de sa préparation, son utilisation et compositions pharmaceutiques la contenant

Country Status (13)

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US (1) US4405608A (fr)
EP (1) EP0047149B1 (fr)
JP (1) JPS5775960A (fr)
AT (1) ATE6639T1 (fr)
AU (1) AU542310B2 (fr)
CA (1) CA1174972A (fr)
DE (1) DE3162674D1 (fr)
DK (1) DK366380A (fr)
ES (1) ES8204713A1 (fr)
GR (1) GR74351B (fr)
NZ (1) NZ198184A (fr)
PT (1) PT73587B (fr)
ZA (1) ZA815094B (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6019719A (ja) * 1983-07-15 1985-01-31 Asahi Chem Ind Co Ltd 抗腫瘍活性を有する蛋白質
WO2009033819A2 (fr) * 2007-09-11 2009-03-19 Mondobiotech Laboratories Ag Utilisation d'un peptide en tant qu'agent thérapeutique
JP6769643B1 (ja) * 2020-04-30 2020-10-14 東海物産株式会社 イミダゾールジペプチドの精製方法
US20240010562A1 (en) * 2022-07-08 2024-01-11 Bmic Llc Rubber-containing asphalt compositions and related methods

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB931954A (en) * 1959-09-14 1963-07-24 Armour Pharma Improvements in or relating to the preparation of crystalline insulin
US3720657A (en) * 1968-11-20 1973-03-13 Organon Process for extracting pancreas glands
US3878186A (en) * 1973-05-09 1975-04-15 Lilly Co Eli Process for purifying insulin
US3907676A (en) * 1970-07-28 1975-09-23 Novo Terapeutisk Labor As Process for purifying insulin
EP0000439A1 (fr) * 1977-07-08 1979-01-24 Laboratorios Leo S.A. Procédé de purification d'un extrait éthanolique aqueux brut contenant de l'insuline obtenu à partir de glandes pancréatiques
GB2038340A (en) * 1978-12-26 1980-07-23 Lilly Co Eli Process for purifying insulin

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US81681A (en) * 1868-09-01 Pcters
US2115418A (en) * 1936-06-03 1938-04-26 Lester R Dragstedt Pancreas-derived product and process of producing it
US3803305A (en) * 1962-12-28 1974-04-09 Rolland A Lab Process for obtaining extracts from pancreas
US3493399A (en) * 1967-04-11 1970-02-03 Ezra Levin Pancreatic food product

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB931954A (en) * 1959-09-14 1963-07-24 Armour Pharma Improvements in or relating to the preparation of crystalline insulin
US3720657A (en) * 1968-11-20 1973-03-13 Organon Process for extracting pancreas glands
US3907676A (en) * 1970-07-28 1975-09-23 Novo Terapeutisk Labor As Process for purifying insulin
US3878186A (en) * 1973-05-09 1975-04-15 Lilly Co Eli Process for purifying insulin
EP0000439A1 (fr) * 1977-07-08 1979-01-24 Laboratorios Leo S.A. Procédé de purification d'un extrait éthanolique aqueux brut contenant de l'insuline obtenu à partir de glandes pancréatiques
GB2038340A (en) * 1978-12-26 1980-07-23 Lilly Co Eli Process for purifying insulin

Also Published As

Publication number Publication date
AU7470681A (en) 1982-03-04
US4405608A (en) 1983-09-20
ATE6639T1 (de) 1984-03-15
JPS5775960A (en) 1982-05-12
ES504996A0 (es) 1982-05-16
DK366380A (da) 1982-03-01
AU542310B2 (en) 1985-02-14
PT73587B (en) 1982-11-10
PT73587A (en) 1981-09-01
ZA815094B (en) 1982-08-25
NZ198184A (en) 1985-08-16
DE3162674D1 (en) 1984-04-19
CA1174972A (fr) 1984-09-25
GR74351B (fr) 1984-06-26
EP0047149B1 (fr) 1984-03-14
ES8204713A1 (es) 1982-05-16

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