EP0109416A1 - Procede et appareil de distribution, pipetage et dilution de fluides. - Google Patents

Procede et appareil de distribution, pipetage et dilution de fluides.

Info

Publication number
EP0109416A1
EP0109416A1 EP83901609A EP83901609A EP0109416A1 EP 0109416 A1 EP0109416 A1 EP 0109416A1 EP 83901609 A EP83901609 A EP 83901609A EP 83901609 A EP83901609 A EP 83901609A EP 0109416 A1 EP0109416 A1 EP 0109416A1
Authority
EP
European Patent Office
Prior art keywords
pump
dispensing
tube
pipetting
fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP83901609A
Other languages
German (de)
English (en)
Other versions
EP0109416A4 (fr
EP0109416B1 (fr
Inventor
Gizella Balas
Janos Csikos
Gabor Kemeny
Zsolt Szabo
Ferenc Vadasz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
"LABOR" MUSZERIPARI MUVEK
MUESZERIPARI MUEVEK LAB
Original Assignee
"LABOR" MUSZERIPARI MUVEK
MUESZERIPARI MUEVEK LAB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by "LABOR" MUSZERIPARI MUVEK, MUESZERIPARI MUEVEK LAB filed Critical "LABOR" MUSZERIPARI MUVEK
Publication of EP0109416A1 publication Critical patent/EP0109416A1/fr
Publication of EP0109416A4 publication Critical patent/EP0109416A4/fr
Application granted granted Critical
Publication of EP0109416B1 publication Critical patent/EP0109416B1/fr
Expired legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Definitions

  • the invention relates to a method and apparatus to dispense, pipet and, afterwards, to dilute fluids, primarily for analytical laboratory use.
  • Patent 4141469 The rare peristaltic pump type dispensers require a separately operated pump head for each dispensing channel. In order to dispense more than one reagent, precision syringe and displacement mechanism is needed for each additional channel. The cost requirement in this latter case can be multiple compared to the price of the multichannel pristaltic pumps.
  • the main object of this invention is to eliminate the defficiencies of the known solutions and to provide a method and an apparatus, with which a fluid can be dispensed, pipetted and diluted in a more convenient and precise way.
  • a method was sought that enables a multichannel peristaltic pump to carry out laboratory fluid dispensing, pipetting and diluting by means of its functionally independent channels.
  • a constant stream of the fluid is established and maintained in a part of a tube by means of a peristaltic pump, and a predetermined amount of the fluid is sipped or dispensed by opening a suction or pressure side of the tube for an adequate time.
  • each channel of the peristaltic pump sips one reagent solution and returns it to the adequate container, with a constant flow rate determined by the measurements of the flexible pump tubing and the velocity of the pump rollers.
  • This said handling procedure of the fluid can be carried out by switching these constant streams by controlled valves.
  • An essential feature of the invention is in that the timing signal for the control unit is provided by a chopper fastened to the pump drive axis, thus an additional pump motor speed control is no more necessary.
  • the pulsation of the peristaltic pump is not a bar, since the pulsation can be synchronized with the timing signal by appropriate selection of the chopper. timing.
  • the well known recycling of the reagent is here not applied for separating the channels of a peristaltic pump, nor for fluid dispensing through this means.
  • the connection into the sipping side of the recycling ring and the pipetting function by this means are also novel. As the pipetting volume in most cases is smaller by orders of magnitude than the diluent volume, it is necessary to combine the pipetting and diluting functions.
  • Fig. 1 is a schematic block diagram of one embodiment in this invention
  • Fig. 2 shows another embodiment
  • Fig. 3 illustrates a further embodiment of the invention.
  • the apparatus shown in Fig. 1 has a peristaltic pump 1, a fluid container 2, a controlled valve 3a, tubings 4b, 5a, 5b, 6 and a control unit 7. With this system, fluid dispensing can be carried out according to this invention.
  • FIG. 2 An apparatus capable to carry out pipetting is shown in Fig. 2, where an additional controlled switch-valve 3b is built into the sipping side of the pump 1, i.e. into tube 4a.
  • Fig. 3 the embodiment of this invention for pipetting and consecutively diluting is shown.
  • Tubes 4d and 5d are connected to another channel of the peristaltic pump 1.
  • the peristaltic pump 1 delivers the contents of container 2 through tubes 4a, 5a towards controlled valve 3a, which is closed towards tube 6 and open towards tube 5b before the dispensing cycle.
  • controlled valve 3a which is closed towards tube 6 and open towards tube 5b before the dispensing cycle.
  • the valve 3 is switched over for a certain period of time determined by the control unit 7 the selected amount of liquid leaves the dispensing tube 6.
  • the timing signal for the control unit 7 is given by a chooper fixed to the axis of the peristaltic pump 1.
  • the frequency of the impulses is preferably unit multiple of the pulsation frequency of the pump, which is known to emanate from the rollers that lift off from the tubes one after the other.
  • the exact time of the valve switching has. to be selected in such a manner that it occurs in all cases at the minimum point of the pulsation.
  • the minimum dispensable volume for each tube diameter of the pump is given by the distance of the rollers that is the "length" of the pressure pulses.
  • the frequency of the signal chopper and, thus, the minimum dispensable volume is limited only by the general technical parameters.
  • the channels of a multichannel peristaltic pump can independently be equipped with controlled valves as it shown in the Figure.
  • each channel can be used for dispensing independently from each others without disturbing the even rotation of the pump.
  • a switch-valve 3b connected into the suction side of the pump 1.
  • Switch-valve 3b connects the suction side tube 4a either through tube 4b to the fluid container 2 or, in the other setting of the valve 3b, through tube 6b to the pipet and outlet tube 6c.
  • the timing signal arrives from the pump 1 and serves as a basis for the control of both valves.
  • the pipetting cycle of the system filled already with the fluid is as follows:
  • the pump 1 is circulating the fluid through tubes 4a, 4b, 5a, 5b.
  • the pipet tip 6c which contains some microliter air gap (see last step of the cycle) is immersed into the fluid sample to be pipetted.
  • the control unit 7 switches over valve 3b for a short time, or for an appropriate number of pulses and, thus, the desired amount of sample will flow into tube 6c.
  • the sipping is finished and the pump works through tubes 4b and 5b again.
  • the pipet tip 6c is placed above the appropriate vessel and dispensing can be started. This occurs by switching over the valve 3a in the same manner as described with Fig. 1.
  • the active time of the valves 3a and 3b is equal.
  • the pipet tip 6c can be flushed by a small amount of fluid from inside.
  • the flush fluid if required serves for the dilution of the sample.
  • a little amount of air can be sucked by switching over valve 3b for a short time, while the tip is hold in the air.
  • the total pipetting cycle time may be too long whtn using the arrangement in Fig.2.
  • the embodiment of Fig. 3, built up in a different manner, while functionally is equiva- lent to that of Fig. 2.
  • the rate of outflow is determined by the cross section of the pump tube 4a, 5a, whereas the inflow sipping rate is determined by the delivery rate of the pump tube 4d, 5d.
  • the method according to the present invention makes it possible to utilize a multichannel peristaltic pump and more controlled valves to build different systems and establish further additional operational functions. Thus, automatic titration will be possible with the system on
  • Fig. 1 if an end point indicator is connected to the control unit.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Reciprocating Pumps (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Procédé et appareil permettant de distribuer, pipeter et ensuite diluer des fluides, destinés essentiellement à un usage analytique en laboratoire. Dans le procédé de cette invention, un courant constant du fluide est établi et maintenu dans une partie d'un tube à l'aide d'une pompe péristaltique, et une quantité prédéterminée de fluide est fournie ou distribuée en ouvrant un côté aspiration ou pression du tube pendant une période de temps appropriée. Avec l'appareil, des tubes côté pression de la pompe sont connectés par l'intermédiaire de soupapes commandées par commutateur à un conteneur de fluide ou à un tube de distribution, et une unité de commande est fixée aux soupapes à commutateur et est connectée électriquement à un détecteur d'un hacheur fixé à un axe de la pompe.
EP19830901609 1982-05-19 1983-05-19 Procede et appareil de distribution, pipetage et dilution de fluides Expired EP0109416B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU159082A HU183961B (en) 1982-05-19 1982-05-19 Method and apparatus for measuring, pipetting and diluting following that fluid quantities
HU159082 1982-05-19

Publications (3)

Publication Number Publication Date
EP0109416A1 true EP0109416A1 (fr) 1984-05-30
EP0109416A4 EP0109416A4 (fr) 1984-09-14
EP0109416B1 EP0109416B1 (fr) 1988-05-18

Family

ID=10955183

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19830901609 Expired EP0109416B1 (fr) 1982-05-19 1983-05-19 Procede et appareil de distribution, pipetage et dilution de fluides

Country Status (5)

Country Link
EP (1) EP0109416B1 (fr)
DE (1) DE3376680D1 (fr)
HU (1) HU183961B (fr)
SU (1) SU1276257A3 (fr)
WO (1) WO1983004015A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU659153B2 (en) * 1991-05-20 1995-05-11 Langoulant, Bruce Le Ber Beverage dispensing apparatus
DE19616869A1 (de) * 1996-04-26 1997-11-06 Hubert Goederle Vorrichtung zur automatisierten Ausgabe von fließfähigen Medien, insbesondere von Getränken
DE19616868A1 (de) * 1996-04-26 1997-11-06 Hubert Goederle Vorrichtung zur automatisierten Ausgabe von fließfähigen Medien, insbesondere von Getränken
ATE552507T1 (de) 2009-04-30 2012-04-15 Hoffmann La Roche System und verfahren zum pipettieren von fluiden, verfahren zum kalibrieren des systems
WO2022044827A1 (fr) * 2020-08-27 2022-03-03 ソニーグループ株式会社 Dispositif d'évacuation de liquide, procédé de commande d'évacuation de liquide, et procédé de remplissage

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3419358A (en) * 1965-10-19 1968-12-31 Technicon Corp Automatic analysis apparatus and method
US3900289A (en) * 1971-04-12 1975-08-19 Abbott Lab Apparatus and method for filling a compartment
US3982667A (en) * 1975-11-24 1976-09-28 Hyperion Incorporated Diluting liquid samples

Also Published As

Publication number Publication date
SU1276257A3 (ru) 1986-12-07
EP0109416A4 (fr) 1984-09-14
HU183961B (en) 1984-06-28
WO1983004015A1 (fr) 1983-11-24
DE3376680D1 (en) 1988-06-23
EP0109416B1 (fr) 1988-05-18

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