EP0176014A2 - Procédé et dispositif pour mélanger un échantillon d'un liquide à analyser - Google Patents

Procédé et dispositif pour mélanger un échantillon d'un liquide à analyser Download PDF

Info

Publication number: EP0176014A2
Authority: EP; European Patent Office
Prior art keywords: air; sample; liquid; cannulas; mixing
Prior art date: 1984-09-22
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

EP85111601A

Other languages

German (de)

English (en)

Other versions

EP0176014B1 (fr

EP0176014A3 (en

Inventor

Peter Scheffler

Wilfried Mauser

Thomas Grazianski

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Eppendorf SE

Original Assignee

Eppendorf Netheler Hinz GmbH

Eppendorf Geraetebau Netheler and Hinz GmbH

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-09-22

Filing date

1985-09-13

Publication date

1986-04-02

1985-09-13 Application filed by Eppendorf Netheler Hinz GmbH, Eppendorf Geraetebau Netheler and Hinz GmbH filed Critical Eppendorf Netheler Hinz GmbH

1985-09-13 Priority to AT85111601T priority Critical patent/ATE44888T1/de

1986-04-02 Publication of EP0176014A2 publication Critical patent/EP0176014A2/fr

1987-04-29 Publication of EP0176014A3 publication Critical patent/EP0176014A3/de

1989-07-26 Application granted granted Critical

1989-07-26 Publication of EP0176014B1 publication Critical patent/EP0176014B1/fr

Status Expired legal-status Critical Current

Links

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Classifications

- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/407—Mixers using gas or liquid agitation, e.g. with air supply tubes by blowing gas on the material from above

Definitions

the invention relates to a method for mixing a liquid sample to be analyzed, in which the liquid sample is introduced into a sample container, in particular into a cuvette, and mixed by periodic air movement.
the invention further relates to a device for mixing a liquid sample to be analyzed contained in a sample container, in particular a cuvette, in which an air movement can be generated above the sample surface.
a method of the type mentioned at the outset is designed according to the invention in such a way that air jets which shift the respective sample surface areas and generate turbulence in the liquid sample are directed alternately to different areas of the sample surface, the air jets preferably having a higher temperature than the sample liquid.
the movement and thus the mixing of the sample liquid takes place solely by alternately directing air jets onto different surface areas of the samples and thereby causing the sample liquid to move in a wave-like manner and thus to generate turbulence which results in rapid mixing of the sample .
the sample container which can be a standard vessel with a single column of liquid, and / or to make essential parts of the device with which the air jets are directed onto the sample surface in contact with the sample container or the sample bring, so that an effective and quick mixing of the liquid sample is achieved in a simple manner.
the evaporation of sample liquid when carrying out the mixing process can be further reduced by using air with a humidity of about 100% for the air jets, so that this saturated air does not absorb any moisture from the sample liquid.
the object on which the invention is based is also achieved with a device of the type mentioned at the outset by at least two air cannulas with their outlet openings above the sample container, which are connected to a pump device which alternately applies air pulses.
the mixing of a liquid sample to be analyzed can be carried out very easily without the opening of the sample container having to be sealed and without there being any contact between parts of the device and the sample container and / or the liquid sample must come.
the air to be supplied to the air cannulas can be conducted through a temperature chamber which can be heated in a controlled manner.
the air to be supplied to the air cannulas can be conducted through a humidification device, which can consist, for example, of a water bowl provided at the bottom of the temperature control chamber, through which the air is conducted.
a humidification device which can consist, for example, of a water bowl provided at the bottom of the temperature control chamber, through which the air is conducted.
the length and the clear cross section of the air cannulas can be chosen so that laminar air flows emerge from them.
the sample container can be covered with an air passage opening for the passage of the air jets, for example in the form of a film attached to the sample container or a resilient cover held on the device.
the device shown contains a temperature control chamber 3 and a pump 12 driven by a drive 20, which is connected to the temperature control chamber 3 via connecting hoses 10 and 11.
the pump 12 has two pump chambers 18, 19, each of which is closed with a membrane 16, 17.
This eccentric is seated on the shaft 13 driven by the drive 20 (not described in more detail) and is rotated in the direction of the arrow shown in FIG.
the connecting hoses 10 and 11 are each connected to one of the chambers 18, 19 and lead in the tempering chamber 3 in subchambers 6, 7, and at a short distance above their bottoms. Air cannulas 4, 5 extend downward through these floors from the temperature control chamber 3, while the upper ends of the air cannulas 4, 5 end relatively far above the connections for the connecting hoses 10 and 11 in the subchambers 6 and 7.
the temperature control chamber 3 is surrounded by an electric heating jacket 26, and in its wall there is a temperature sensor 27, by means of which the heating of the temperature control chamber is selected via a schematically illustrated temperature controller 8 so that the desired temperature is maintained in the subchambers 6, 7 .
the air cannulas 4, 5 are led out downward from the temperature control chamber 3 in such a way that when a sample vessel 1 is positioned below the outlet openings of the air cannulas 4 and 5 they are in the area of the clear opening cross section of the sample container 1, but at the greatest possible distance from one another, while this Sample vessel 1 is covered by a cover 22, which is resiliently held on the temperature control chamber 3 and has through openings 23 for the air jets.
the drive 20 drives the shaft 13 and thereby rotates the eccentric 14, as a result of which air is compressed periodically in the chambers 18 and 19 (FIG. 2) and thereby displaced from it and pressed into the subchambers 6 and 7 via the connecting hoses 10 and 11 .
Water can be located at the bottom of these subchambers, so that the air supplied to the subchambers flows over this water and is humidified, while the air in the subchambers 6 and 7 is also heated to a temperature which is above the temperature of the sample to be mixed .
the dimensions of the air cannulas 4 and 5 are preferably chosen so that laminar flows emerge from them.
air cannulas with a length of 40 mm and a clear inner diameter of 0.8 were used for an exit velocity of the air jets of 1 m / sec to 2 m / sec with a volume of 5 cm 3 / sec to 10 cm 3 / sec mm used.
the laminar flow produced in this way resulted in effective mixing if the outlet ends of the air cannulas 4, 5 were 21 mm to 30 mm above the sample surface.
a sample container 1 for example a cuvette, containing a liquid sample 2 to be mixed is placed under the air cannulas 4 and 5, as is indicated in FIGS. 1 and 3.
an air jet indicated by an arrow in FIG. 1
this air jet in the laterally offset, 1 'designated sample container, which is identical to the sample container 1, causes a deformation of the surface of the liquid sample designated 2', as indicated in Figure 1.
the surface of the liquid is thus alternately deformed test in connection with turbulence indicated by curved arrows in the manner indicated in Figure 1, which leads to a quick and effective mixing of the liquid sample, whereby due to the increased temperature and the high humidity of the air jets neither a temperature change nor a loss of liquid in the liquid sample is to be feared is.

Landscapes

Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Sampling And Sample Adjustment (AREA)
Automatic Analysis And Handling Materials Therefor (AREA)
Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)

EP85111601A 1984-09-22 1985-09-13 Procédé et dispositif pour mélanger un échantillon d'un liquide à analyser Expired EP0176014B1 (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
AT85111601T ATE44888T1 (de)	1984-09-22	1985-09-13	Verfahren und vorrichtung zum mischen einer zu analysierenden fluessigkeitsprobe.

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE3434931		1984-09-22
DE19843434931 DE3434931A1 (de)	1984-09-22	1984-09-22	Verfahren und vorrichtung zum mischen einer zu analysierenden fluessigkeitsprobe

Publications (3)

Publication Number	Publication Date
EP0176014A2 true EP0176014A2 (fr)	1986-04-02
EP0176014A3 EP0176014A3 (en)	1987-04-29
EP0176014B1 EP0176014B1 (fr)	1989-07-26

Family

ID=6246151

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP85111601A Expired EP0176014B1 (fr)	1984-09-22	1985-09-13	Procédé et dispositif pour mélanger un échantillon d'un liquide à analyser

Country Status (5)

Country	Link
US (1)	US4664526A (fr)
EP (1)	EP0176014B1 (fr)
JP (1)	JPS6180026A (fr)
AT (1)	ATE44888T1 (fr)
DE (2)	DE3434931A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0281958A3 (fr) *	1987-03-07	1989-03-22	Hoechst Aktiengesellschaft	Dispositif de régulation de température et de mélange du contenu d'une plaque de micro titration
EP0589363A1 (fr) *	1992-09-25	1994-03-30	Boehringer Mannheim Gmbh	Procédé et dispositif de mélange automatique sans contact d'un mélange de réaction dans un dispositif d'analyse
WO1994019097A1 (fr) *	1993-02-26	1994-09-01	MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V.	Procede et dispositif permettant de melanger des liquides dans des plaques de microtitrage
US9298397B2 (en)	2013-11-12	2016-03-29	Globalfoundries Inc.	Nonvolatile storage thresholding for ultra-SSD, SSD, and HDD drive intermix

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH04506729A (ja) *	1989-04-28	1992-11-19	ファースト　パシフィック　ネットワークス，インコーポレイテッド	時間及び周波数多重化を使用した分散型インテリジェンスネットワーク
US5595707A (en)	1990-03-02	1997-01-21	Ventana Medical Systems, Inc.	Automated biological reaction apparatus
US6565533B1 (en) *	2000-01-21	2003-05-20	Novus International, Inc.	Inoculation apparatus and method
US8323984B2 (en) *	2002-12-19	2012-12-04	Beckman Coulter, Inc.	Method and apparatus for mixing blood samples for cell analysis
US7648678B2 (en)	2002-12-20	2010-01-19	Dako Denmark A/S	Method and system for pretreatment of tissue slides
DE102004028303A1 (de) *	2004-06-11	2005-12-29	Roche Diagnostics Gmbh	Verfahren und Vorrichtung zum Nachweis von Analyten
DE102005034175A1 (de) *	2005-07-21	2007-01-25	Roche Diagnostics Gmbh	Verfahren und Vorrichtung zur Erzeugung eines Feuchtluftstromes mit definierter relativer Feuchte
EP2838650B1 (fr)	2012-04-18	2019-09-25	Life Technologies Corporation	Procédés et appareil pour transfert de masse entre un flux de gaz et un liquide
DE102012023900A1 (de)	2012-12-07	2014-06-12	Meiko Maschinenbau Gmbh & Co. Kg	Förderaggregat

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US2171399A (en) *	1934-10-24	1939-08-29	Louis Peters	Method and apparatus for homogenizing powdered mixtures
US2193002A (en) *	1939-09-07	1940-03-12	Ish-Shalom Samu-El	Convertible mixing apparatus
US3087840A (en) *	1958-06-16	1963-04-30	Macrosonic Process Corp	Methods and means for producing physical, chemical and physicochemical effects by large-amplitude sound waves
US3398935A (en) *	1964-03-25	1968-08-27	Bausch & Lomb	Mixing means
CH478410A (de) *	1966-10-11	1969-09-15	Greiner Electronic Ag	Verfahren zur Bestimmung der Blutgerinnung
JPS4820078U (fr) *	1971-07-15	1973-03-07
BE788877A (fr) *	1971-09-17	1973-01-02	Vickers Ltd	Agitation d'echantillons liquides pour l'obtention de melanges homogenes
JPS58137758A (ja) *	1982-02-10	1983-08-16	Omron Tateisi Electronics Co	血液撹拌装置
DE3367336D1 (en) *	1982-07-01	1986-12-11	Eppendorf Geraetebau Netheler	Method for mixing fluid samples to be analysed
DE3578454D1 (de) *	1984-02-01	1990-08-02	Baxter Int	Klinisches analysesystem und verfahren.

1984
- 1984-09-22 DE DE19843434931 patent/DE3434931A1/de active Granted
1985
- 1985-09-13 JP JP60203218A patent/JPS6180026A/ja active Granted
- 1985-09-13 AT AT85111601T patent/ATE44888T1/de active
- 1985-09-13 EP EP85111601A patent/EP0176014B1/fr not_active Expired
- 1985-09-13 DE DE8585111601T patent/DE3571768D1/de not_active Expired
- 1985-09-18 US US06/777,334 patent/US4664526A/en not_active Expired - Fee Related

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0281958A3 (fr) *	1987-03-07	1989-03-22	Hoechst Aktiengesellschaft	Dispositif de régulation de température et de mélange du contenu d'une plaque de micro titration
EP0589363A1 (fr) *	1992-09-25	1994-03-30	Boehringer Mannheim Gmbh	Procédé et dispositif de mélange automatique sans contact d'un mélange de réaction dans un dispositif d'analyse
US5362147A (en) *	1992-09-25	1994-11-08	Boehringer Mannheim Gmbh	Method and apparatus for the contactless automatic mixing of a reaction mixture in an analysis unit
WO1994019097A1 (fr) *	1993-02-26	1994-09-01	MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V.	Procede et dispositif permettant de melanger des liquides dans des plaques de microtitrage
US9298397B2 (en)	2013-11-12	2016-03-29	Globalfoundries Inc.	Nonvolatile storage thresholding for ultra-SSD, SSD, and HDD drive intermix

Also Published As

Publication number	Publication date
ATE44888T1 (de)	1989-08-15
DE3434931C2 (fr)	1987-10-08
JPH0578779B2 (fr)	1993-10-29
DE3571768D1 (en)	1989-08-31
DE3434931A1 (de)	1986-03-27
EP0176014B1 (fr)	1989-07-26
US4664526A (en)	1987-05-12
JPS6180026A (ja)	1986-04-23
EP0176014A3 (en)	1987-04-29

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1986-02-14	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
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1988-11-23	RAP1	Party data changed (applicant data changed or rights of an application transferred)	Owner name: EPPENDORF-NETHELER-HINZ GMBH
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Publication	Publication Date	Title
EP0176014B1 (fr)	1989-07-26	Procédé et dispositif pour mélanger un échantillon d'un liquide à analyser
DE970926C (de)	1958-11-13	Vorrichtung zum Mischen, Ruehren usw. von Fluessigkeiten
DE69014423T2 (de)	1995-09-07	Verarbeitungsgerät für ein Gefäss für chemische Reaktionen.
DE2245711A1 (de)	1973-03-29	Verfahren und vorrichtung zum ruehren eines probenmaterials
DE1673103C3 (fr)	1975-10-23
DE2251409A1 (de)	1973-04-26	Verfahren und vorrichtung zur fluessigkeitsentfernung aus behaeltern
CH463024A (de)	1968-09-30	Verfahren und Vorrichtung zum Desinfizieren von Geräten und Instrumenten
DE2612568A1 (de)	1976-09-30	Vorrichtung zum verduennen und zum umruehren einer fluessigkeit
DE2552883C2 (fr)	1987-08-20
DE2200730C3 (de)	1978-06-22	Einrichtung zum Abmessen und Verteilen einer Vielzahl von kleinen Flüssigkeitsmengen
DE3333600C2 (de)	1984-07-26	Wasserheizvorrichtung der Wärmepumpenart
DE2329348B2 (de)	1976-07-01	Verfahren und vorrichtung zum aufteilen einer reihe fluessiger proben in einer fluidleitungsanordnung zur analyse der proben
DE2537606C3 (de)	1980-11-27	Anordnung zum automatischen Transportieren und Injizieren einer Flüssigkeitsprobe
DE1902200B1 (de)	1970-11-26	Vorrichtung zum Mischen von Stoffen
DE3879731T2 (de)	1993-10-14	Vorrichtung zum tropfenweisen Aufsaugen und Abgeben einer Flüssigkeit.
DE102006007542B3 (de)	2007-09-20	Verfahren und Vorrichtung zum Ansaugen eines Flüssigkeitsvolumens, insbesondere zur Entnahme einer Probe zur Analyse mittels einer Flüssigkeitschromatographievorrichtung
CH674317A5 (fr)	1990-05-31
DE2552833B2 (de)	1979-02-22	Optischer Schnellanalysator vom Drehküvettentyp
EP0367917A2 (fr)	1990-05-16	Appareil pour l'application au choix d'une aqua puncture ou d'une hydro-ionisation
DE2329914A1 (de)	1974-01-17	Heizvorrichtung
CH615356A5 (en)	1980-01-31	Apparatus for uniform spraying of thin-layer plates with reagents for quantitative thin-layer chromatography
DE3803981C2 (fr)	1989-11-16
DE2703755A1 (de)	1978-08-03	Vorrichtung zum mischen von mehrkomponentenkunststoffen, insbesondere polyurethan
DE2208226A1 (de)	1973-09-13	Mischer mit oberflaechenwechselwirkung
DE2208362B2 (de)	1974-06-06	Verfahren und Einrichtung zum Aufbringen eines strichförmigen Auftrags auf einem Trägermaterial