EP0344163A1 - Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii - Google Patents

Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii

Info

Publication number
EP0344163A1
EP0344163A1 EP88900367A EP88900367A EP0344163A1 EP 0344163 A1 EP0344163 A1 EP 0344163A1 EP 88900367 A EP88900367 A EP 88900367A EP 88900367 A EP88900367 A EP 88900367A EP 0344163 A1 EP0344163 A1 EP 0344163A1
Authority
EP
European Patent Office
Prior art keywords
xylose
xylitol
medium
yeast
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP88900367A
Other languages
German (de)
English (en)
Inventor
Heikki Ojamo
Lasse Ylinen
Matti Linko
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Farmos Yhtyma Oy
Original Assignee
Farmos Yhtyma Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Farmos Yhtyma Oy filed Critical Farmos Yhtyma Oy
Publication of EP0344163A1 publication Critical patent/EP0344163A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida

Definitions

  • the object of the present invention is a microbiological process for the preparation of xylitol from xylose con ⁇ taining mixtures using the yeast strain Candida guillier ⁇ mondii VTT-C-71006.
  • Xylitol is conventionally prepared on a technical scale by hydrolysing hemicellulose containing substances, for example birch wood, whereby a pentose rich hydrolysate is obtained.
  • the xylose of the hydrolysate can thereafter be hydrogenated catalytically to form xylitol.
  • microbiological methods for the production of xylitol are prior known.
  • H. Onishi & T. Suzuki "The Production of Xylitol, L-Arabinitol and Ribitol by Yeasts", Agr. Biol. Chem., Vol. 30, No. 11, p. 1139 to 1144, 1966, is disclosed the microbiological production of xylitol from a xylose containing solution by the yeast species Candida guilliermondii.
  • the initial xylose con ⁇ centration of the solution used as raw material was 10 % by weight.
  • the yield of xylitol was 40.3 %.
  • the strain Candida guilliermondii VTT-C-71006 is deposited in the strain collection at National Collection of Yeast Cultures (NCYC), Norwich, England, on 17th December 1986 under no NCYC-1644.
  • Candida guilliermondii VTT-C-71006 exhibits the following morphological and physiological properties.
  • cultivation is carried out by cultivating the strain Candida guilliermon- dii VTT-C-71006 preferably under aerated conditions in a xylose containing medium having a xylose concentration va ⁇ rying from 5 to 50 % by weight at a temperature of 20 to 40°C for a period ranging from 20 hours to 10 days.
  • a xylose containing medium having a xylose concentration va ⁇ rying from 5 to 50 % by weight at a temperature of 20 to 40°C for a period ranging from 20 hours to 10 days.
  • Suitable raw materials are any xylose containing mixtures, for example the xylose rich mixtures obtained from the hydrolysis of hemicellulose containing materials.
  • the fermentation solution as the N-source for the yeast different ammonium salts may be used and as a growth fac ⁇ tor source for example yeast extract, yeast produced in the process or extract of plant origin. Essential is the restriction of the oxygen supply to the yeast.
  • xylitol is obtained at an amount as high as 78 %.
  • the pro ⁇ cess is slowed down at very high initial xylose concentra ⁇ tions.
  • the process may, however, be accelerated compared to a normal batch process even by 40 % by using so called fed-batch cultivation, which means that during the pro ⁇ cess, nutrients are added to the fermenter.
  • the cultivation was carried out using different xylose concentrations and different aeration efficiences in both a fermenter and in agitated cultures.
  • composition of the medium was xylose 40 to 300 g/1 yeast extract 10 g/1
  • furfural favourably affected the yield of xylitol.
  • the presence of furfural in microbiological processes has been considered purely disadvantageous as furfural inhibits the growth of yeast.
  • the inhibition of the growth of the yeast is as such desirable in processes of this kind, as the growth of yeast consumes raw materi ⁇ als which is manifested in a decrease of the amount of xy ⁇ litol.
  • furfural did not completely inhibit the action of the yeast, but acted as a factor promoting the production of xylitol.
  • furfural usually disturbs both the growth and the fermentation process (for example N. Banerjee et al., European J. Appl. Microbiol. Biotechnol. 1981, 11:226-228)) .
  • furfural for the promotion of microbiological processes is not limited to the production of xylitol from xylose by means of the process described above. It is evi ⁇ dent that this observation may be applied also to other microbiological processes, the object of which is not the production of the microbe as such but the production of substances independent of the microbial growth, for examp ⁇ le in biotransformations.
  • the yeast concentration was deter ⁇ mined by measuring the turbidity, the xylose concentration by determining the concentration of reducing substances and the formation of xylitol was determined gualitatively by using thin layer chromatography and quantitatively by using liquid chromatography or an enzymatic method.
  • the process according to the invention exhibits many ad ⁇ vantages with respect to the known processes. Of these may be mentioned for example: - High xylitol yield, 50 to 78 %, depending on the xylo ⁇ se concentration.
  • the process mentioned earlier and be ⁇ longing to the state of art gave a xylitol yield of on ⁇ ly 40.3 % although the initial xylose concentration was only 10 %.
  • the yield obtained was 74 %.
  • the xylitol produced with this method is pure and is thus quite suitable for use in foodstuffs.
  • Xylitol pro ⁇ may contain residues of toxic catalyst.
  • the method according to the invention is also more economical, as the catalysts are expensive and are consumed in the process.
  • the strain Candida guilliermondii VTT-C-71006 is grown aseptically while agitating in a solution volume of 100 ml in a 250 ml vessel.
  • the growth temperature is 28°C.
  • the medium contains 80 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone.
  • the pH of the medium was adjusted to a value of 5.0.
  • the xylose was determined as reducing sugars and the xyli ⁇ tol using liquid chromatograph . The xylitol yield was thus 62.5 %.
  • Example 2 The method according to the Example 1 was repeated, the initial xylose concentration being 300 g/1. After 6 days the xylose was consumed completely and the xylitol con ⁇ centration was 150 g/1, which corresponds to a yield of 50 %.
  • Example 5 The strain mentioned in the Example 1 is grown aseptically in a fermenter in a medium containing 100 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone. The pH of the medium was adjusted to a value of 5.0 and the stirring speed to 400 rp and the aeration to about 0.5 1/min. Af ⁇ ter 50 hours of cultivation the xylose was totally consu ⁇ med and the xylitol concentration was 74 g/1 (yield 74 %) .
  • Example 5 Example 5
  • Example 4 The process of Example 4 was repeated but using 250 g/1 as the initial xylose concentration. After cultivating for 160 hours, the xylose was completely consumed and the xy ⁇ litol concentration was 138 g/1 (yield 55 %) .
  • the strain mentioned in the previous example is grown in a fermenter as a fed-batch-culture.
  • the initial volume of the culture solution was 60 % of the end volume and its composition was 100 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone. Cultivation was started as in the Example 4, but when the xylose concentration had decreased to a value of 40 g/1, the introduction of a xylose solut ⁇ ion at a concentration of 350 g/1 was begun. By means of the addition the xylose concentration in the fermenter was kept at 40 g/1. When the final volume was reached the ad ⁇ dition was stopped. After cultivating for 120 hours the xylose was completely consumed and the xylitol concentrat ⁇ ion was 164 g/1, which corresponds to a xylitol yield of

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procédé microbiologique de préparation de xylitol à partir de mélanges contenant du xylose. La souche de levure Candida guilliermondii VTT-C-71006 est cultivée, de préférence dans des conditions d'aération limitée, dans un milieu riche en xylose. On obtient une excellente production de xylitol en ajoutant au milieu du furfural en une quantité comprise entre environ 0,2 et 1,0 g/l. Pendant la fermentation, on utilise soit un procédé par lots conventionnel soit un procédé par lots à alimentation.
EP88900367A 1987-01-15 1987-12-08 Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii Withdrawn EP0344163A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI870147A FI76377C (fi) 1987-01-15 1987-01-15 Mikrobiologisk framstaellningsmetod.
FI870147 1987-01-15

Publications (1)

Publication Number Publication Date
EP0344163A1 true EP0344163A1 (fr) 1989-12-06

Family

ID=8523778

Family Applications (1)

Application Number Title Priority Date Filing Date
EP88900367A Withdrawn EP0344163A1 (fr) 1987-01-15 1987-12-08 Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii

Country Status (4)

Country Link
EP (1) EP0344163A1 (fr)
JP (1) JPH02502333A (fr)
FI (1) FI76377C (fr)
WO (1) WO1988005467A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641545A1 (fr) * 1989-01-10 1990-07-13 Agrocinq Procede de biosynthese du xylitol
ZA90254B (en) * 1989-01-17 1990-11-28 Cultor Oy Method for the production of xylitol
FI86440C (fi) 1990-01-15 1992-08-25 Cultor Oy Foerfarande foer samtidig framstaellning av xylitol och etanol.
US7109005B2 (en) 1990-01-15 2006-09-19 Danisco Sweeteners Oy Process for the simultaneous production of xylitol and ethanol
FI913197A0 (fi) 1991-07-01 1991-07-01 Xyrofin Oy Nya jaeststammar med reducerad foermaoga att metabolisera xylitol, foerfarande foer bildande av dessa och deras anvaendning vid framstaellning av xylitol.
US7226761B2 (en) 1992-11-05 2007-06-05 Danisco Sweeteners Oy Manufacture of five-carbon sugars and sugar alcohols
EP0672161B1 (fr) * 1992-11-05 1999-09-22 Xyrofin Oy Production de xylitol par recombinaison et hote utilise pour ce procede
US6723540B1 (en) 1992-11-05 2004-04-20 Xyrofin Oy Manufacture of xylitol using recombinant microbial hosts
FR2720406B1 (fr) * 1994-05-27 1996-08-14 Agronomique Inst Nat Rech Composition de microorganismes et procédé pour la production de xylitol.
US6224776B1 (en) 1996-05-24 2001-05-01 Cultor Corporation Method for fractionating a solution
FI20010977L (fi) 2001-05-09 2002-11-10 Danisco Sweeteners Oy Kromatografinen erotusmenetelmä
FI20011889A7 (fi) 2001-09-26 2003-03-27 Xyrofin Oy Menetelmä ksylitolin valmistamiseksi
JP2010104361A (ja) * 2008-10-02 2010-05-13 Musashino Chemical Laboratory Ltd リグノセルロース系バイオマスを用いた糖化液の製造方法
CN110382684A (zh) 2016-12-21 2019-10-25 创新生物科学公司 产生木糖醇的梅奇酵母种

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3619369A (en) * 1968-10-31 1971-11-09 Noda Inst For Scientific Res Process for producing xylitol by fermentation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO8805467A1 *

Also Published As

Publication number Publication date
WO1988005467A1 (fr) 1988-07-28
FI76377B (fi) 1988-06-30
FI76377C (fi) 1989-05-10
FI870147A0 (fi) 1987-01-15
JPH02502333A (ja) 1990-08-02

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