EP0368926A4 - Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same - Google Patents

Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same

Info

Publication number
EP0368926A4
EP0368926A4 EP19880907466 EP88907466A EP0368926A4 EP 0368926 A4 EP0368926 A4 EP 0368926A4 EP 19880907466 EP19880907466 EP 19880907466 EP 88907466 A EP88907466 A EP 88907466A EP 0368926 A4 EP0368926 A4 EP 0368926A4
Authority
EP
European Patent Office
Prior art keywords
obtaining
methods
same
transfected cells
cells containing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19880907466
Other languages
English (en)
Other versions
EP0368926A1 (fr
Inventor
Linda M. Cashion
Kathi A. Begley
Wendy W. Colby
Michael John Morser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Berlex Laboratories Inc
Original Assignee
Codon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codon filed Critical Codon
Publication of EP0368926A1 publication Critical patent/EP0368926A1/fr
Publication of EP0368926A4 publication Critical patent/EP0368926A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP19880907466 1987-07-16 1988-07-15 Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same Withdrawn EP0368926A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7408387A 1987-07-16 1987-07-16
US74083 1993-06-08

Publications (2)

Publication Number Publication Date
EP0368926A1 EP0368926A1 (fr) 1990-05-23
EP0368926A4 true EP0368926A4 (en) 1991-11-27

Family

ID=22117617

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19880907466 Withdrawn EP0368926A4 (en) 1987-07-16 1988-07-15 Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same

Country Status (3)

Country Link
EP (1) EP0368926A4 (fr)
AU (1) AU2263288A (fr)
WO (1) WO1989000605A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3744595A1 (de) * 1987-12-31 1989-07-13 Andreas Dr Plueckthun Verfahren zur gentechnischen herstellung von antikoerpern
DE4410188A1 (de) * 1994-03-24 1995-09-28 Boehringer Mannheim Gmbh Gentherapeutisches Verfahren unter Verwendung von antibiotikaresistenzgenfreien DNA-Vektoren
GB9603803D0 (en) * 1996-02-22 1996-04-24 Zeneca Ltd Production of proteins, plasmids coding therefor and organisms containing such plasmids
EP1308517A1 (fr) * 2001-10-31 2003-05-07 Aventis Pharmacueticals Products Inc. Vecteurs pour l'expression de multiples transgenes
PT2592148T (pt) 2007-10-12 2018-11-12 Hoffmann La Roche Expressão proteica de múltiplos ácidos nucleicos
JP7021245B2 (ja) 2017-03-10 2022-02-16 エフ．ホフマン－ラ ロシュ アーゲー 多重特異性抗体を生産するための方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0048202A2 (fr) * 1980-09-11 1982-03-24 Merck & Co. Inc. Méthode de préparation d'une lignée cellulaire d'hépatome exempte de mycoplasmes
EP0113319A2 (fr) * 1982-12-30 1984-07-11 Elaine Lynette Dr. Wilson Lignées cellulaires et leur utilisation dans la production de protéines
EP0211260A1 (fr) * 1985-07-10 1987-02-25 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Séquence d'ADN chromosomique, vecteur d'expression pour facteur activant le plasminogène du tissu humain, cellules cultivées transfectées avec ledit facteur et méthode de production dudit facteur activant
US4663281A (en) * 1984-03-22 1987-05-05 Mass Institute Of Technology Enhanced production of proteinaceous materials in eucaryotic cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0192658A4 (fr) * 1984-07-30 1987-07-13 Salk Inst For Biological Studi Vecteurs retroviraux de transfert genetique.
EP0216846B2 (fr) * 1985-04-01 1995-04-26 Celltech Limited Lignee cellulaire de myelomes transformee et procede d'expression d'un gene codant un polypeptide eucaryotique employant cette lignee

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0048202A2 (fr) * 1980-09-11 1982-03-24 Merck & Co. Inc. Méthode de préparation d'une lignée cellulaire d'hépatome exempte de mycoplasmes
EP0113319A2 (fr) * 1982-12-30 1984-07-11 Elaine Lynette Dr. Wilson Lignées cellulaires et leur utilisation dans la production de protéines
US4663281A (en) * 1984-03-22 1987-05-05 Mass Institute Of Technology Enhanced production of proteinaceous materials in eucaryotic cells
EP0211260A1 (fr) * 1985-07-10 1987-02-25 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Séquence d'ADN chromosomique, vecteur d'expression pour facteur activant le plasminogène du tissu humain, cellules cultivées transfectées avec ledit facteur et méthode de production dudit facteur activant

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GENE, vol. 49, no. 1, 1986, pages 1-8, Elsevier, Amsterdam, NL; M. DREANO et al.: "High level, heatregulated synthesis of proteins in eukaryotic cells" *
GENE, vol. 64, no. 1, 15h April 1988, pages 43-51, Elsevier, Amsterdam, NL; M.B. HENDRICKS et al.: "A high-efficiency vector for expression of foreign genes in myeloma cells" *
J. NATL. CANCER INST., vol. 59, no. 1, July 1977, pages 221-226; J. FOGH et al.: "One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice" *
MOLECULAR AND CELLULAR BIOLOGY, vol. 5, no. 12, December 1985, pages 3403-3409, American Society for Microbiology; S. CHAKRABARTI et al.: "Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques" *
See also references of WO8900605A1 *

Also Published As

Publication number Publication date
EP0368926A1 (fr) 1990-05-23
WO1989000605A1 (fr) 1989-01-26
AU2263288A (en) 1989-02-13

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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Effective date: 19900111

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Owner name: BERLEX LABORATORIES, INC.

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Effective date: 19911011

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Effective date: 19930923