EP0455043A2 - Dispositif de pipettage - Google Patents

Dispositif de pipettage Download PDF

Info

Publication number
EP0455043A2
EP0455043A2 EP91106075A EP91106075A EP0455043A2 EP 0455043 A2 EP0455043 A2 EP 0455043A2 EP 91106075 A EP91106075 A EP 91106075A EP 91106075 A EP91106075 A EP 91106075A EP 0455043 A2 EP0455043 A2 EP 0455043A2
Authority
EP
European Patent Office
Prior art keywords
pipetting device
jacket
piston
carrier
cylinder
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP91106075A
Other languages
German (de)
English (en)
Other versions
EP0455043B1 (fr
EP0455043A3 (en
Inventor
Horst Sabloewski
Ingo Cordes
Jochen Beese
Klaus Schürbrock
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf Netheler Hinz GmbH
Eppendorf Geraetebau Netheler and Hinz GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Netheler Hinz GmbH, Eppendorf Geraetebau Netheler and Hinz GmbH filed Critical Eppendorf Netheler Hinz GmbH
Publication of EP0455043A2 publication Critical patent/EP0455043A2/fr
Publication of EP0455043A3 publication Critical patent/EP0455043A3/de
Application granted granted Critical
Publication of EP0455043B1 publication Critical patent/EP0455043B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops

Definitions

  • the invention relates to a pipetting device according to the preamble of claim 1.
  • the first-mentioned version of the pipetting device has the task of creating an improvement in that a one-hand operation is possible not only without having to change the piston movement in accordance with the calibrated pipetting volume, an additional blow-out movement and also for moving the housing sleeve for the purpose of ejecting a pipette tip, but also The load on the hand holding the device is reduced to a minimum, but in order to increase the operating safety, signs of reaching certain movement or functional distances can also be perceived.
  • a piston is continuously rod-shaped, that is to say solid, and is arranged in such a way that it enters tightly into a cylinder which leads tubularly to a connecting cone for an attachable pipette tip.
  • This is a rod-shaped piston for pipetting very small volumes, the piston diameter being practically only about 1 to 5 mm.
  • a piston rod is connected to the piston via a connecting component, which in this embodiment is also rod-shaped and has a small cross-section, so that it can easily get into the lower connecting piece.
  • a connecting component which in this embodiment is also rod-shaped and has a small cross-section, so that it can easily get into the lower connecting piece.
  • the invention relates to a pipetting device in which the piston has a larger cross section than the tubular connecting opening to the connecting cone for a plug-on pipette tip.
  • the present invention is based on the assumption that different pipetting volumes are to be processed and, for this purpose, a special cylinder space, which is referred to above as a cylinder, is present above the tubular connecting channel to the connecting cone, which can already be referred to as a cylinder for the smallest volumes and which is arranged with a larger cross section. Accordingly, the piston has a larger cross section than the tubular connection.
  • the plunger itself is adjustable in the cylinder to adapt to different pipetting volumes, ie when it is displaced a smaller amount of air in the sense of a smaller pipetting volume, the piston is advanced a corresponding distance into the cylinder space by adjusting the actuating element, so that it is then moved over the remaining axial length of the cylinder space when the pipetting stroke is carried out.
  • a pipetting device of the type mentioned at the outset is known from DE-AS 1 952 774.
  • the piston of this pipette is provided with an extension covered by a sleeve made of a fluorocarbon, which is operatively connected to an O-ring for sealing against the cylinder wall.
  • the piston extension is firmly overlaid by the fluorocarbon sleeve and is apparently glued or pressed onto it. This requires a relatively complex manufacturing process and does not preclude relative changes in the position of the sleeve and extension with prolonged use with a concomitant reduction in pipetting accuracy with the desirable safety. Vibrations or shocks can affect the intake volume via the actuating element.
  • the piston cannot be guided sealingly on a cylinder running surface with reasonable effort, so that the O-ring should also serve to compensate for tolerances. Due to the rubber O-ring or another suitable sealing material, the sensitivity of the pipette to wear is relatively high. Another disadvantage is that the material of the piston does not match those to be processed Substances can be adapted and the pipette must be replaced as a whole in the event of unacceptable wear on the piston running surface.
  • the object of the invention is to improve a pipetting device of the type mentioned at the outset in such a way that it is easier to manufacture, the pipetting accuracy remains the same even under changing operating conditions and interferences, the wear sensitivity is reduced with improved guidance and sealing, and an adaptation to that processing substances or a compensation of wear effects is possible without changing the entire pipetting device.
  • a hollow piston with a precision jacket is an advantageous embodiment in several respects, because the outer piston running material can be reduced and then a very low thickness is present if, for example, a steel jacket is used.
  • the inner carrier there is a compensating or deviating influencing variable with regard to heat conduction, so that with a particularly thin version of the tubular Jacket made of a good heat-conducting material nevertheless creates thermal insulation between the handle part of the pipetting device and its end of work.
  • the training as a hollow piston also allows the exchange of the piston jacket depending on the substances to be processed.
  • the ring seals create a structure that not only enables the tubular jacket to be replaced in a favorable manner, but also makes assembly easy, the ring seals also allowing adjustments when the piston is guided or run into a narrow cylinder.
  • the jacket according to claim 4 is made of ceramic. This is advantageous or important for special applications. It can be assumed here that liquids to be processed can emit aggressive gases, especially if acids or other aggressive liquids with a higher vapor pressure are processed.
  • the interchangeable design of the carrier is an expedient embodiment, wherein an adaptation is also possible with different jacket thicknesses using otherwise the same parts. This is a real easier installation.
  • a Claim 9 contains a particularly advantageous embodiment, which enables simple implementation of the invention, including some of the features mentioned above.
  • the disc-like element mentioned above can be removable.
  • claim 12 results in an advantageous solution for independent adjustment of the piston guide.
  • claim 13 contains favorable features.
  • claim 14 provides an inexpensive solution, especially for constant guidance and easy assembly, because parts are also saved, namely separate seals.
  • the pipetting device shown in the union of FIGS. 1 to 3 has a housing 1, which can be composed of several parts, but is designated 1 in the entire area.
  • an actuating element 2 designed as a push button is arranged at the upper end, which can be moved against a spring 3, but can also be rotated, so that the volume of the pipetting device can be changed via the setting and drive means 4 specified thereby.
  • a spindle 6 is rotated by the connection inserts 5 shown, so that a linkage 7 is thereby advanced.
  • a carrier 9 for a piston 10 is arranged by means of a connecting piece 8.
  • This piston can be displaced into a cylinder 11 by which leads a tubular connection 12 to the connecting cone 13.
  • the purpose of the spring 14 shown in Fig. 1 is to return the actuator.
  • the spring 15 has the purpose of returning the piston to its starting position and, above all, of maintaining the connection between a holding device, for example formed by a magnet 16 and a movable counter-magnetic part 17.
  • the spring 18 merely replaces a sleeve in order to press a lower guide bush 19 for the piston 10 against an inner conical inclined surface 21 of the housing 1 by means of a flexible annular seal 20.
  • FIG. 3 shows a solution which is not the subject of the present invention, for example has an axially displaceable housing sleeve 22, by means of which plug-on vessels can be thrown off.
  • the piston 10 has the configuration according to the invention as a hollow piston. It has the outer tubular jacket 23 and the inner carrier 9, which is made of a heat-insulating plastic, and has two annular grooves 24, 25, in which annular seals 26, 27 are arranged are. Depending on the material to be processed, these can be rubber seals or resistant elastic plastic seals. These guide the tubular jacket 23, which in the advantageous embodiment is arranged with a sliding fit on the inner carrier 9.
  • the carrier is connected by means of extension 28 to the connecting piece 8 by screwing or plug connection.
  • This connector 8 consists in particular of heat-insulating material. It can be elastic and has an outwardly directed flange as an abutment 29, against which the upper edge of the tubular jacket 23 abuts.
  • the connector has its functional features not only in the material, but also in the type of easy suspension and assembly, ie that the support 9 for the piston 10 should also be interchangeable.
  • the connecting piece has an upwardly directed bushing 40 which surrounds the rod 7 and which, with slots distributed around the circumference, has inwardly directed axial webs which adapt themselves to the rod and, in accordance with the guidance of the piston 10, have an adaptable one Form a connection.
  • the cohesion is in the area the socket is secured by an outer, in particular metallic bandage 41, which can be detachably attached if the interchangeability of the plug connection between the attachment 28 and a downwardly directed cylinder web 42 of the connecting piece is sufficient on the one hand for securing the connection but on the other hand for releasable cohesion.
  • the lower edge of the tubular jacket is pressed by a particularly disc-like element 30, as a crosspiece on the support 9, by means of the support connection to the connecting piece 8. This is connected to the linkage 7 upwards.
  • the tubular jacket 23 has a strength that it can be made of ceramic.
  • the carrier 9 When the carrier 9 is releasably used, it can be exchanged by means of the releasable extension 28, the division of the housing 1 into the upper handle part 31 and a lower part 32, which are joined together by a threaded connection 33, in particular being accommodated.
  • the cylinder 11 has an inner space with a diameter 34.
  • the piston 10 has an outer diameter 35. It is also included in relation to the tubular connection 12 that the difference between the diameters 34 and 35 is very small, advantageously of the order of four Is tenths of a millimeter.
  • the disc-like element 30 with the carrier 9, but also its passage in one piece to the linkage 7 can be seen.
  • the support 9 in this embodiment also has the annular seals 26 and 27. If the support for the linkage 7 passes in one piece in this way, then special precautions are provided for the upper abutment for the piston skirt, which ultimately must be movable with the linkage. From this point of view, the linkage can have a section 36 with a thread 37 onto which a flange 38 is screwed. The thread also allows an appropriate setting.
  • FIG. 4 also shows a special configuration in the area of the seal 26, in the area of which the carrier 9 is shown in sections in section. It can be seen that the seal 26 is formed in one piece on the support 9 or linkage 7. The same can also apply to the seal 27.
  • linkage 7 and support 9 consist of a plastic suitable for seals, which has sufficient strength for the intended use.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Catching Or Destruction (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Pipe Accessories (AREA)
  • Endoscopes (AREA)
EP91106075A 1990-05-04 1991-04-17 Dispositif de pipettage Expired - Lifetime EP0455043B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4014333 1990-05-04
DE4014333A DE4014333A1 (de) 1990-05-04 1990-05-04 Pipettiervorrichtung

Publications (3)

Publication Number Publication Date
EP0455043A2 true EP0455043A2 (fr) 1991-11-06
EP0455043A3 EP0455043A3 (en) 1992-02-26
EP0455043B1 EP0455043B1 (fr) 1995-05-24

Family

ID=6405711

Family Applications (1)

Application Number Title Priority Date Filing Date
EP91106075A Expired - Lifetime EP0455043B1 (fr) 1990-05-04 1991-04-17 Dispositif de pipettage

Country Status (5)

Country Link
US (1) US5511433A (fr)
EP (1) EP0455043B1 (fr)
JP (1) JPH0655278B2 (fr)
AT (1) ATE122930T1 (fr)
DE (2) DE4014333A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0562358A3 (en) * 1992-03-25 1994-05-18 Eppendorf Geraetebau Netheler Pipetting system
DE4335863C1 (de) * 1993-10-21 1995-02-02 Eppendorf Geraetebau Netheler Kolbenhubpipette
US5454268A (en) * 1993-11-15 1995-10-03 Kim; Young S. Double-plunger liquid displacement syringe pipet
EP2626134A3 (fr) * 2012-02-07 2013-11-06 Eppendorf AG Pipette
CN117619468A (zh) * 2022-08-30 2024-03-01 艾本德欧洲股份有限公司 柱塞杆和用于制作这种柱塞杆的方法

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI88775C (fi) * 1992-02-11 1993-07-12 Biohit Oy Pipett
FI109407B (fi) * 2001-02-09 2002-07-31 Biohit Oyj Imulaite
JP4148894B2 (ja) 2001-10-16 2008-09-10 マトリックス・テクノロジイズ・コーポレーション 手持ち式ピペット
US20060027033A1 (en) * 2002-10-16 2006-02-09 Richard Cote Hand-held pipette employing voice recognition control
US7284454B2 (en) * 2004-05-27 2007-10-23 Matrix Technologies Corporation Hand held pipette
USD510629S1 (en) 2004-01-16 2005-10-11 Heathrow Scientific Llc Pipette device with pivotable nozzle assembly
US7381371B2 (en) * 2004-01-16 2008-06-03 Heathrow Scientific Llc Pipette device with pivotable nozzle assembly
US20080199361A1 (en) * 2007-02-16 2008-08-21 Francis Gomes Hand-held pipetting device
US20090010809A1 (en) * 2007-07-03 2009-01-08 Hadjis Peter T Manual pipette filler
US20090007701A1 (en) * 2007-07-03 2009-01-08 Hadjis Peter T Pivoting pipette device
US8337782B2 (en) * 2007-10-16 2012-12-25 Ivek Corporation Coupling system for use with fluid displacement apparatus
PL214640B1 (pl) * 2009-04-30 2013-08-30 Pz Htl Spolka Akcyjna Zespól trzonu do zamontowania w pipecie mechanicznej lub elektronicznej
US9322750B2 (en) * 2012-03-09 2016-04-26 Torion Technologies, Inc. Needle trap for use with a gas chromatograph-mass spectrometer for field sampling and analysis
US9358538B2 (en) 2012-04-30 2016-06-07 The Regents Of The University Of Michigan High resolution pipette
JP2016500549A (ja) 2012-11-05 2016-01-14 オースティン バイオイノベーション インスティテュート イン アクロンＡｕｓｔｅｎ Ｂｉｏｉｎｎｏｖａｔｉｏｎ Ｉｎｓｔｉｔｕｔｅ Ｉｎ Ａｋｒｏｎ 低容量シリンジピペット
US10076750B2 (en) * 2014-02-04 2018-09-18 Austen Bioinnovation Institute In Akron Plunger for low-volume syringe pipette
FI3932557T3 (fi) * 2020-07-02 2023-12-28 Eppendorf Se Monikanavainen pipettipää

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE858148C (de) * 1943-03-27 1952-12-04 Josef Gries Vorrichtung zum Ansaugen einer fuer die Bestimmung der Blutsenkung erforderlichen Blutmenge
US3506164A (en) * 1968-05-31 1970-04-14 Sherwood Medical Ind Inc Automatic pipette
US3646817A (en) * 1968-10-25 1972-03-07 Bio Dynamics Inc Pipette
US3732735A (en) * 1971-06-17 1973-05-15 Medical Laboratory Automation Pipettes
US3853012A (en) * 1971-10-04 1974-12-10 Medical Laboratory Automation Pipettes
DE2319175A1 (de) * 1973-04-16 1974-10-31 Oxford Lab Detail-kolbenpipette
US3901413A (en) * 1974-03-01 1975-08-26 Precision Sampling Corp High pressure syringe
SE381577B (sv) * 1974-06-24 1975-12-15 Mediplast Ab Anordning for overforing av en vetska, sasom blod fran en cylindrisk behallare till en pipett
SU542547A1 (ru) * 1974-09-12 1977-01-15 Институт Биохимии И Физиологии Микроорганизмов Ан Ссср Пипетка
DE2549477C3 (de) * 1975-11-05 1982-01-07 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Pipettiervorrichtung
FR2351402A1 (fr) * 1976-05-10 1977-12-09 Marteau Dautry Eric Diluteur reglable
DE2651333C3 (de) * 1976-11-10 1980-10-16 Walter Sarstedt Kunststoff-Spritzgusswerk, 5223 Nuembrecht Saugpipette
JPS5919736B2 (ja) * 1981-02-09 1984-05-08 株式会社ニチリヨ− ピペット装置
US4616514A (en) * 1983-06-06 1986-10-14 Rainin Instrument Co., Inc. Replaceable tip assembly for pipette
JPS60222571A (ja) * 1984-04-18 1985-11-07 Toshiba Corp シリンダ−ポンプ
DE3875117D1 (de) * 1988-10-21 1992-11-05 Eppendorf Geraetebau Netheler Pipettiervorrichtung.

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0562358A3 (en) * 1992-03-25 1994-05-18 Eppendorf Geraetebau Netheler Pipetting system
DE4335863C1 (de) * 1993-10-21 1995-02-02 Eppendorf Geraetebau Netheler Kolbenhubpipette
US5454268A (en) * 1993-11-15 1995-10-03 Kim; Young S. Double-plunger liquid displacement syringe pipet
EP2626134A3 (fr) * 2012-02-07 2013-11-06 Eppendorf AG Pipette
CN117619468A (zh) * 2022-08-30 2024-03-01 艾本德欧洲股份有限公司 柱塞杆和用于制作这种柱塞杆的方法
EP4331725A1 (fr) * 2022-08-30 2024-03-06 Eppendorf SE Tige de piston et procédé de fabrication d'une telle tige de piston
US12594549B2 (en) 2022-08-30 2026-04-07 Eppendorf Se Plunger rod and method for producing such a plunger rod

Also Published As

Publication number Publication date
US5511433A (en) 1996-04-30
ATE122930T1 (de) 1995-06-15
EP0455043B1 (fr) 1995-05-24
JPH04227859A (ja) 1992-08-17
JPH0655278B2 (ja) 1994-07-27
DE4014333A1 (de) 1991-11-28
DE59105556D1 (de) 1995-06-29
EP0455043A3 (en) 1992-02-26
DE4014333C2 (fr) 1992-07-02

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